MD07G1040400.v1.1

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000989
Physical Location & Seq
Forward (+)
3400868 .. 3405091
4224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1040400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCCATGATACCGGAAAGCGATGGAATATGTGAGGCTGTTAGAATACAGCCTTATCTTGTCGATGCTGATGGACATTGCTTTTGGAAACTGAAAGGCGATTTTGGTGAAGATATATTGCTTCAAGATTTGGGAACCTGGGATGGAACTCCATCTGGTGAACAACGGTCCGTCTGCAGTGCTAAAGAGCCAATAGACAAATACCGCTCTTCCCCGGCTCTAAAGGAAAAACAGGCCAAGAGGGAGCGAAGGCATTCCAAATACTTGCAGCTTTCCCAAACTTCTTCGGAAAGCAATAATAAAAATGCGCAGGTTTCCCAGACTCCTGATAGCAATGGCAAAAAGGGGCAGGTTTCCCAAGCTCCTGAATGCAATCGAGAGAACATGGAGCTCGGTCCTGAGGTGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

137

Amino Acids

15.14

Weight (kDa)

5.44

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 309
Acc36I ACCTGC 2 cut(s) 301, 340
AccBSI CCGCTC 1 cut(s) 207
AciI CCGC 1 cut(s) 205
AcoI YGGCCR 1 cut(s) 3
AgsI TTSAA 1 cut(s) 125
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 3 cut(s) 271, 362, 391
AluI AGCT 3 cut(s) 271, 362, 391
Alw21I GWGCWC 1 cut(s) 393
AoxI GGCC 2 cut(s) 3, 234
ApeKI GCWGC 1 cut(s) 268
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
Asp700I GAANNNNTTC 1 cut(s) 253
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 2 cut(s) 168, 395
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 119, 170
AvaII GGWCC 2 cut(s) 168, 395
AxyI CCTNAGG 1 cut(s) 399
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 280
BccI CCATC 4 cut(s) 17, 65, 137, 160
BciT130I CCWGG 1 cut(s) 139
BcnI CCSGG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 175
BfuAI ACCTGC 2 cut(s) 301, 340
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 2 cut(s) 139, 215
Bme18I GGWCC 2 cut(s) 168, 395
BmgT120I GGNCC 2 cut(s) 168, 395
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 2 cut(s) 139, 215
BmsI GCATC 1 cut(s) 55
BpuMI CCSGG 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 138, 213
BsaWI WCCGGW 1 cut(s) 13
Bse21I CCTNAGG 1 cut(s) 399
Bse3DI GCAATG 2 cut(s) 76, 340
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 138, 213
BseGI GGATG 1 cut(s) 148
BseMI GCAATG 2 cut(s) 76, 340
BseMII CTCAG 1 cut(s) 390
BseXI GCAGC 1 cut(s) 280
BshFI GGCC 2 cut(s) 5, 236
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 2 cut(s) 14, 215
BsmI GAATGC 2 cut(s) 253, 374
BsnI GGCC 2 cut(s) 5, 236
Bsp1286I GDGCHC 1 cut(s) 393
BspACI CCGC 1 cut(s) 205
BspANI GGCC 2 cut(s) 5, 236
BspCNI CTCAG 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 136
BspMAI CTGCAG 1 cut(s) 179
BspMI ACCTGC 2 cut(s) 301, 340
BspQI GCTCTTC 1 cut(s) 214
BsrBI CCGCTC 1 cut(s) 207
BsrDI GCAATG 2 cut(s) 76, 340
BssECI CCNNGG 2 cut(s) 138, 213
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 168
Bst6I CTCTTC 1 cut(s) 214
BstDEI CTNAG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 148
BstHHI GCGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 137, 213
BstSFI CTRYAG 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 2 cut(s) 5, 236
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 1 cut(s) 148
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 184
BveI ACCTGC 2 cut(s) 301, 340
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 168, 395
CpoI CGGWCCG 1 cut(s) 168
CspI CGGWCCG 1 cut(s) 168
CviAII CATG 2 cut(s) 7, 385
CviJI RGCY 9 cut(s) 5, 38, 52, 190, 218, 236, 271, 362, 391
CviKI_1 RGCY 9 cut(s) 5, 38, 52, 190, 218, 236, 271, 362, 391
DdeI CTNAG 1 cut(s) 399
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 214
Ecl136II GAGCTC 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 393
Eco47I GGWCC 2 cut(s) 168, 395
Eco53kI GAGCTC 1 cut(s) 391
Eco81I CCTNAGG 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 393
FaeI CATG 2 cut(s) 10, 388
FaiI YATR 4 cut(s) 8, 31, 116, 386
FatI CATG 2 cut(s) 6, 384
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 1 cut(s) 269
FspI TGCGCA 1 cut(s) 309
GlaI GCGC 1 cut(s) 309
GluI GCNGC 1 cut(s) 269
HaeIII GGCC 2 cut(s) 5, 236
HapII CCGG 2 cut(s) 14, 215
HhaI GCGC 1 cut(s) 310
Hin1II CATG 2 cut(s) 10, 388
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HinfI GANTC 1 cut(s) 322
HpaII CCGG 2 cut(s) 14, 215
HphI GGTGA 2 cut(s) 119, 170
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 5 cut(s) 125, 326, 365, 377, 398
Hpy8I GTNNAC 1 cut(s) 161
HpyAV CCTTC 1 cut(s) 243
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4V TGCA 3 cut(s) 177, 268, 372
HpyF3I CTNAG 1 cut(s) 399
Hsp92II CATG 2 cut(s) 10, 388
HspAI GCGC 1 cut(s) 308
LguI GCTCTTC 1 cut(s) 214
LmnI GCTCC 3 cut(s) 244, 367, 388
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 1 cut(s) 55
MbiI CCGCTC 1 cut(s) 207
MboII GAAGA 3 cut(s) 122, 201, 276
MhlI GDGCHC 1 cut(s) 393
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 406
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 316
MnlI CCTC 3 cut(s) 28, 234, 394
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 253
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 409
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 14, 215
MspR9I CCNGG 2 cut(s) 139, 215
Mva1269I GAATGC 2 cut(s) 253, 374
MvaI CCWGG 1 cut(s) 139
NciI CCSGG 1 cut(s) 215
NlaIII CATG 2 cut(s) 10, 388
NlaIV GGNNCC 1 cut(s) 136
NsbI TGCGCA 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 214
PctI GAATGC 2 cut(s) 253, 374
PdmI GAANNNNTTC 1 cut(s) 253
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 2 cut(s) 168, 395
PstI CTGCAG 1 cut(s) 179
Rsr2I CGGWCCG 1 cut(s) 168
RsrII CGGWCCG 1 cut(s) 168
SacI GAGCTC 1 cut(s) 393
SapI GCTCTTC 1 cut(s) 214
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 1 cut(s) 269
Sau96I GGNCC 2 cut(s) 168, 395
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 139, 215
SduI GDGCHC 1 cut(s) 393
SetI ASST 7 cut(s) 140, 273, 315, 354, 364, 393, 405
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 175
SinI GGWCC 2 cut(s) 168, 395
Sse9I AATT 1 cut(s) 406
SsiI CCGC 1 cut(s) 205
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 2 cut(s) 137, 213
TaaI ACNGT 1 cut(s) 168
TaqI TCGA 2 cut(s) 63, 376
TaqII GACCGA 1 cut(s) 383
TasI AATT 1 cut(s) 406
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TscAI CASTG 1 cut(s) 184
TseI GCWGC 1 cut(s) 268
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 184
VpaK11BI GGWCC 2 cut(s) 168, 395
XmnI GAANNNNTTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.