MD17G1074500.v1.1

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000989
Physical Location & Seq
Reverse (-)
6045587 .. 6049491
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1074500.v1.1.491

Sequence Viewer

Length: 1875 bp
ATGGCAATAGATTCGAGCTCTGCTGTTGAGAGGATGGATGTGGAGGTCAACTATCTTGCGGAGAGCAACGTGTTTGTGCAGGACCATGACGGCCCGGCCGTCGGAGGCCAGATCGCCGGTTCTAAGAACGGAACCTGTCACCAGTGCCGTCAGAGGAAGACGGAATTCGCAGCGTCGTGTACAAACGTCAAAGCGAAAGGCCCTTGCACGGTTAAGCTATGCCATAGTTGTCTGAAGAACAGGTATACATTGACGGCTGAGCAAGTGGCAGAGAAATGTCCGAAATGTCGAGGCGAATGTAATTGCAGTATATGCCATAAGAAAGCCGGTTTAATGCCCACTGGTATTCGCACACACGAAGCCAAGAGGAAAGGGTATCAATCTGTTGCCGATATGTTGATTGTGGAGAAGCCGCAAAAGAAGGAAAATGGCGACAGCAAGGGTGGTAACGGGCAGGAGCGTGTGGCTTCAGACAAGGCAACGGGTGTTGATTCACCTAGCAAACGAGGGAAGGAGGATGCCCTTGATCAGAGTGAAGATGTGAACTCGGATGCAGCAAAAGAGGATGAGTTGATAGAAACTTGTGATGACGGCAGAGATGGTGGTGCTGCTGTGAATGACAATGATGCGAAGGCCAAGGGCAAGGCTGCAAGAGGGCGTCACAGGGTCAAGGAACATTGCATGGAGAGCAGAGTGGTTGTTCCTGAGGTTCCCCTACCTCAGGGCACCCCATTGACAACTGTAGCGGGTGTCGACTTAGCTGCAGAGGATGTGGGAAATGCTCTCCAATTCTTGAAATTTTGTGAAACTTTTAGAGAGTTTTTTAAGATTGAAAAGCCCCAAGCTGAGTCTGTTCTTCGGGAACTATCGCGTGGAGGAAGTGGGCGAAGTGGACGGCCTGGACAGTACTCCTCTGTAGTTCGATTTCATAGCTATATGCTCTTCTTCATACAGAAGGGTTTATTACAGGAGTCTCTTGAGCAAGCAAGCACCAACTGCTCATGGTTCCAAGATTTGGCGAACCTGGTGTCTGCATCCGGGGTCTCTAAGGTCGACCCTGTGATGAAGGAGCTGCCTACAGAGTGTTTTATTGAAGGCGGGGAGGGATACGATGCCTTAAACTTCTCCCAAAAGCTTCAGCTTCTGATTTTCCTCTGCGATGAGGCTCTTAACACAAAGACGCTGAGGGGATGGATTGAGGAGCAAAATGAAAGATCTGTCCAACGTAAAAGGGACATGAAAGCAAAAGTTGCTCTGGCAAAGGATAAGGAGAGAGATCTCCAAGCAAAGTTGCAAGAGCAGATGGCTAAGAGGACCACTGCAGAGAATAGCGATGCTGTCCCAATTTCAGAGTCGAGCATCCTGATTTCAGAGTCGGACTCCATTGCTTCAGTACTCAGAAGTGAGTTAGCTCAAGCTCACATGGAGGTGGTTGAAGCAATGGCCATGGTACCCGAAAGCAATGAAATATGTGAGGCTGTTAGAATAGAGCCCTACATTGTGGATGCTGATGGCCATGCCTTCTGGAAACTGAGAGGCGATTTTGATGAAGATATATTGCTTCAAGGTCTGGGATCCTGGGATGGAACTCCATCTGGTGAACAATGGTTCGTCTGTGGTGCTGAAATGAAAGAGCCAATTGACAAATACCGCTCTTCTCTGGCTCTAAAGGAAAAACAGACCAAGAGGGAGCAAAGGCGTTACAGAACTTCAAGCAATGATGAAAAATTGCAGCTTTCCCAAACTTCTTCGGAAAGCAATGAAGAAAACGCGCAGGGTTCTCAGACTCCTGATAGCAATGGCGAAAGCGTGCAGGCATCCCGAGCTCCTGAATGCAATGAAGAGAACATGGAGCTCGGTCCTTCGGCGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

625

Amino Acids

68.58

Weight (kDa)

5.26

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 43 - 133 8.3e-23 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1450
AccB1I GGYRCC 2 cut(s) 725, 1450
AccB7I CCANNNNNTGG 1 cut(s) 1015
AccBSI CCGCTC 1 cut(s) 1653
AccI GTMKAC 3 cut(s) 245, 753, 1053
AccII CGCG 2 cut(s) 871, 1772
AciI CCGC 5 cut(s) 59, 413, 746, 1098, 1651
AclWI GGATC 2 cut(s) 1569, 1582
AcoI YGGCCR 3 cut(s) 96, 1443, 1513
AcsI RAATTY 2 cut(s) 164, 797
AcuI CTGAAG 4 cut(s) 254, 453, 1121, 1374
AcyI GRCGYC 1 cut(s) 658
AfaI GTAC 4 cut(s) 181, 908, 1395, 1452
AfiI CCNNNNNNNGG 4 cut(s) 101, 208, 721, 1015
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 6 cut(s) 796, 833, 1094, 1436, 1565, 1713
AjnI CCWGG 3 cut(s) 898, 1023, 1577
Alw21I GWGCWC 3 cut(s) 20, 1828, 1857
Alw26I GTCTC 2 cut(s) 978, 1048
AlwI GGATC 2 cut(s) 1569, 1582
AlwNI CAGNNNCTG 1 cut(s) 1144
Ama87I CYCGRG 1 cut(s) 1821
AoxI GGCC 8 cut(s) 91, 96, 106, 199, 633, 896, 1443, 1513
ApeKI GCWGC 7 cut(s) 170, 554, 608, 647, 761, 1072, 1732
ApoI RAATTY 2 cut(s) 164, 797
ArsI GACNNNNNNTTYG 2 cut(s) 1013, 1045
Asp718I GGTACC 1 cut(s) 1450
AspLEI GCGC 1 cut(s) 1774
AspS9I GGNCC 5 cut(s) 82, 92, 200, 1314, 1859
AsuC2I CCSGG 2 cut(s) 95, 1039
AsuHPI GGTGA 3 cut(s) 131, 486, 1610
AvaI CYCGRG 1 cut(s) 1821
AvaII GGWCC 3 cut(s) 82, 1314, 1859
AxyI CCTNAGG 2 cut(s) 705, 720
BaeGI GKGCMC 1 cut(s) 728
BalI TGGCCA 2 cut(s) 1445, 1515
BamHI GGATCC 1 cut(s) 1574
BanI GGYRCC 2 cut(s) 725, 1450
BanII GRGCYC 4 cut(s) 20, 1494, 1828, 1857
BbsI GAAGAC 1 cut(s) 164
Bbv12I GWGCWC 3 cut(s) 20, 1828, 1857
BbvCI CCTCAGC 1 cut(s) 1184
BbvI GCAGC 7 cut(s) 182, 566, 595, 634, 748, 1059, 1744
BccI CCATC 7 cut(s) 28, 593, 1185, 1297, 1505, 1577, 1600
BceAI ACGGC 6 cut(s) 83, 106, 132, 270, 607, 911
BcgI CGANNNNNNTGC 3 cut(s) 28, 743, 777
BciT130I CCWGG 3 cut(s) 900, 1025, 1579
BciVI GTATCC 1 cut(s) 1100
BclI TGATCA 1 cut(s) 526
BcnI CCSGG 2 cut(s) 95, 1039
BcoDI GTCTC 2 cut(s) 978, 1048
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 5 cut(s) 741, 762, 915, 1077, 1320
BfuI GTATCC 1 cut(s) 1100
BglII AGATCT 2 cut(s) 1214, 1276
BisI GCNGC 8 cut(s) 171, 413, 555, 609, 648, 762, 1073, 1733
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 8 cut(s) 172, 414, 556, 610, 649, 763, 1074, 1734
BmcAI AGTACT 2 cut(s) 908, 1395
Bme1390I CCNGG 5 cut(s) 95, 900, 1025, 1039, 1579
Bme18I GGWCC 3 cut(s) 82, 1314, 1859
BmeT110I CYCGRG 1 cut(s) 1821
BmgT120I GGNCC 5 cut(s) 82, 92, 200, 1314, 1859
BmiI GGNNCC 6 cut(s) 133, 711, 727, 1007, 1452, 1576
BmrFI CCNGG 5 cut(s) 95, 900, 1025, 1039, 1579
BmsI GCATC 9 cut(s) 508, 541, 616, 1043, 1102, 1324, 1368, 1495, 1826
BpiI GAAGAC 1 cut(s) 164
BplI GAGNNNNNCTC 2 cut(s) 1364, 1396
Bpu10I CCTNAGC 1 cut(s) 1184
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 2 cut(s) 998, 1398
BpuMI CCSGG 2 cut(s) 95, 1039
BsaHI GRCGYC 1 cut(s) 658
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 4 cut(s) 636, 1038, 1446, 1578
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 208, 721, 1015
Bse118I RCCGGY 2 cut(s) 116, 326
Bse1I ACTGG 2 cut(s) 142, 346
Bse21I CCTNAGG 2 cut(s) 705, 720
Bse3DI GCAATG 8 cut(s) 676, 1383, 1446, 1468, 1723, 1765, 1804, 1843
BseBI CCWGG 3 cut(s) 900, 1025, 1579
BseDI CCNNGG 4 cut(s) 636, 1038, 1446, 1578
BseLI CCNNNNNNNGG 4 cut(s) 101, 208, 721, 1015
BseMI GCAATG 8 cut(s) 676, 1383, 1446, 1468, 1723, 1765, 1804, 1843
BseMII CTCAG 8 cut(s) 249, 696, 734, 837, 1175, 1411, 1523, 1796
BseNI ACTGG 2 cut(s) 142, 346
BseRI GAGGAG 2 cut(s) 901, 1214
BseSI GKGCMC 1 cut(s) 728
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 7 cut(s) 182, 566, 595, 634, 748, 1059, 1744
BsgI GTGCAG 2 cut(s) 98, 1832
Bsh1236I CGCG 2 cut(s) 871, 1772
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 8 cut(s) 93, 98, 108, 201, 635, 898, 1445, 1515
BshNI GGYRCC 2 cut(s) 725, 1450
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 3 cut(s) 20, 1828, 1857
BsiHKCI CYCGRG 1 cut(s) 1821
BsiSI CCGG 4 cut(s) 95, 117, 327, 1038
BslFI GGGAC 2 cut(s) 1247, 1325
BslI CCNNNNNNNGG 4 cut(s) 101, 208, 721, 1015
BsmAI GTCTC 2 cut(s) 978, 1048
BsmFI GGGAC 2 cut(s) 1247, 1325
BsmI GAATGC 1 cut(s) 1838
BsnI GGCC 8 cut(s) 93, 98, 108, 201, 635, 898, 1445, 1515
Bso31I GGTCTC 1 cut(s) 1048
BsoBI CYCGRG 1 cut(s) 1821
Bsp1286I GDGCHC 5 cut(s) 20, 728, 1494, 1828, 1857
Bsp1407I TGTACA 1 cut(s) 179
Bsp143I GATC 5 cut(s) 111, 526, 1214, 1276, 1574
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 1446
BspACI CCGC 5 cut(s) 59, 413, 746, 1098, 1651
BspANI GGCC 8 cut(s) 93, 98, 108, 201, 635, 898, 1445, 1515
BspCNI CTCAG 8 cut(s) 250, 697, 733, 838, 1176, 1410, 1524, 1795
BspFNI CGCG 2 cut(s) 871, 1772
BspLI GGNNCC 6 cut(s) 133, 711, 727, 1007, 1452, 1576
BspMAI CTGCAG 2 cut(s) 766, 1324
BspPI GGATC 2 cut(s) 1569, 1582
BspQI GCTCTTC 2 cut(s) 947, 1660
BspT107I GGYRCC 2 cut(s) 725, 1450
BspTNI GGTCTC 1 cut(s) 1048
BsrBI CCGCTC 1 cut(s) 1653
BsrDI GCAATG 8 cut(s) 676, 1383, 1446, 1468, 1723, 1765, 1804, 1843
BsrFI RCCGGY 2 cut(s) 116, 326
BsrGI TGTACA 1 cut(s) 179
BsrI ACTGG 2 cut(s) 142, 346
BssAI RCCGGY 2 cut(s) 116, 326
BssECI CCNNGG 4 cut(s) 636, 1038, 1446, 1578
BssMI GATC 5 cut(s) 111, 526, 1214, 1276, 1574
BssNAI GTATAC 1 cut(s) 246
BssNI GRCGYC 1 cut(s) 658
BssT1I CCWWGG 2 cut(s) 636, 1446
Bst1107I GTATAC 1 cut(s) 246
Bst2UI CCWGG 3 cut(s) 900, 1025, 1579
Bst4CI ACNGT 3 cut(s) 211, 742, 906
Bst6I CTCTTC 3 cut(s) 947, 1660, 1836
BstACI GRCGYC 1 cut(s) 658
BstAPI GCANNNNNTGC 3 cut(s) 312, 996, 1250
BstAUI TGTACA 1 cut(s) 179
BstC8I GCNNGC 4 cut(s) 984, 988, 1811, 1815
BstDSI CCRYGG 1 cut(s) 1446
BstENI CCTNNNNNAGG 1 cut(s) 719
BstFNI CGCG 2 cut(s) 871, 1772
BstHHI GCGC 1 cut(s) 1774
BstKTI GATC 5 cut(s) 114, 529, 1217, 1279, 1577
BstMAI GTCTC 2 cut(s) 978, 1048
BstMBI GATC 5 cut(s) 111, 526, 1214, 1276, 1574
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 5 cut(s) 312, 687, 996, 1250, 1823
BstNI CCWGG 3 cut(s) 900, 1025, 1579
BstSCI CCNGG 5 cut(s) 93, 898, 1023, 1037, 1577
BstSFI CTRYAG 5 cut(s) 741, 762, 915, 1077, 1320
BstSLI GKGCMC 1 cut(s) 728
BstUI CGCG 2 cut(s) 871, 1772
BstV1I GCAGC 7 cut(s) 182, 566, 595, 634, 748, 1059, 1744
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 3 cut(s) 1214, 1276, 1574
BstYI RGATCY 3 cut(s) 1214, 1276, 1574
BstZ17I GTATAC 1 cut(s) 246
BstZI CGGCCG 1 cut(s) 96
Bsu36I CCTNAGG 2 cut(s) 705, 720
BsuI GTATCC 1 cut(s) 1100
BsuRI GGCC 8 cut(s) 93, 98, 108, 201, 635, 898, 1445, 1515
BtgI CCRYGG 1 cut(s) 1446
BtgZI GCGATG 2 cut(s) 1173, 1347
BtsI GCAGTG 1 cut(s) 1317
BtsIMutI CAGTG 3 cut(s) 149, 339, 1317
Cac8I GCNNGC 4 cut(s) 984, 988, 1811, 1815
CaiI CAGNNNCTG 1 cut(s) 1144
CfoI GCGC 1 cut(s) 1774
Cfr10I RCCGGY 2 cut(s) 116, 326
Cfr13I GGNCC 5 cut(s) 82, 92, 200, 1314, 1859
CseI GACGC 3 cut(s) 162, 647, 1189
CsiI ACCWGGT 1 cut(s) 1023
Csp6I GTAC 4 cut(s) 180, 907, 1394, 1451
CviAII CATG 8 cut(s) 86, 682, 1002, 1237, 1423, 1447, 1517, 1849
CviQI GTAC 4 cut(s) 180, 907, 1394, 1451
DpnI GATC 5 cut(s) 113, 528, 1216, 1278, 1576
DpnII GATC 5 cut(s) 111, 526, 1214, 1276, 1574
EaeI YGGCCR 3 cut(s) 96, 1443, 1513
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 3 cut(s) 947, 1660, 1836
EarI CTCTTC 3 cut(s) 947, 1660, 1836
Ecl136II GAGCTC 3 cut(s) 18, 1826, 1855
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 636, 1446
Eco24I GRGCYC 4 cut(s) 20, 1494, 1828, 1857
Eco31I GGTCTC 1 cut(s) 1048
Eco47I GGWCC 3 cut(s) 82, 1314, 1859
Eco52I CGGCCG 1 cut(s) 96
Eco53kI GAGCTC 3 cut(s) 18, 1826, 1855
Eco57I CTGAAG 4 cut(s) 254, 453, 1121, 1374
Eco81I CCTNAGG 2 cut(s) 705, 720
Eco88I CYCGRG 1 cut(s) 1821
EcoICRI GAGCTC 3 cut(s) 18, 1826, 1855
EcoNI CCTNNNNNAGG 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 3 cut(s) 898, 1023, 1577
EcoT14I CCWWGG 2 cut(s) 636, 1446
EcoT38I GRGCYC 4 cut(s) 20, 1494, 1828, 1857
ErhI CCWWGG 2 cut(s) 636, 1446
FaeI CATG 8 cut(s) 89, 685, 1005, 1240, 1426, 1450, 1520, 1852
FaqI GGGAC 2 cut(s) 1247, 1325
FatI CATG 8 cut(s) 85, 681, 1001, 1236, 1422, 1446, 1516, 1848
FauI CCCGC 2 cut(s) 739, 1091
FbaI TGATCA 1 cut(s) 526
FblI GTMKAC 3 cut(s) 245, 753, 1053
Fnu4HI GCNGC 8 cut(s) 171, 413, 555, 609, 648, 762, 1073, 1733
FriOI GRGCYC 4 cut(s) 20, 1494, 1828, 1857
Fsp4HI GCNGC 8 cut(s) 171, 413, 555, 609, 648, 762, 1073, 1733
FspBI CTAG 1 cut(s) 498
GlaI GCGC 1 cut(s) 1773
GluI GCNGC 8 cut(s) 171, 413, 555, 609, 648, 762, 1073, 1733
HaeIII GGCC 8 cut(s) 93, 98, 108, 201, 635, 898, 1445, 1515
HapII CCGG 4 cut(s) 95, 117, 327, 1038
HgaI GACGC 3 cut(s) 162, 647, 1189
HhaI GCGC 1 cut(s) 1774
Hin1I GRCGYC 1 cut(s) 658
Hin1II CATG 8 cut(s) 89, 685, 1005, 1240, 1426, 1450, 1520, 1852
Hin6I GCGC 1 cut(s) 1772
HinP1I GCGC 1 cut(s) 1772
HincII GTYRAC 3 cut(s) 49, 754, 1054
HindII GTYRAC 3 cut(s) 49, 754, 1054
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 8 cut(s) 11, 491, 848, 971, 1352, 1373, 1379, 1786
HpaII CCGG 4 cut(s) 95, 117, 327, 1038
HphI GGTGA 3 cut(s) 131, 486, 1610
Hpy166II GTNNAC 8 cut(s) 49, 180, 246, 544, 754, 893, 1054, 1601
Hpy188III TCNNGA 9 cut(s) 704, 793, 860, 977, 1363, 1525, 1790, 1821, 1829
Hpy8I GTNNAC 8 cut(s) 49, 180, 246, 544, 754, 893, 1054, 1601
Hpy99I CGWCG 2 cut(s) 104, 178
HpyAV CCTTC 8 cut(s) 415, 505, 625, 949, 1060, 1088, 1531, 1872
HpyCH4III ACNGT 3 cut(s) 211, 742, 906
HpyCH4IV ACGT 3 cut(s) 69, 186, 1225
HpyF10VI GCNNNNNNNGC 5 cut(s) 312, 687, 996, 1250, 1823
HpySE526I ACGT 3 cut(s) 69, 186, 1225
Hsp92I GRCGYC 1 cut(s) 658
Hsp92II CATG 8 cut(s) 89, 685, 1005, 1240, 1426, 1450, 1520, 1852
HspAI GCGC 1 cut(s) 1772
KpnI GGTACC 1 cut(s) 1454
Ksp22I TGATCA 1 cut(s) 526
Kzo9I GATC 5 cut(s) 111, 526, 1214, 1276, 1574
LguI GCTCTTC 2 cut(s) 947, 1660
LmnI GCTCC 6 cut(s) 457, 1069, 1201, 1690, 1831, 1852
Lsp1109I GCAGC 7 cut(s) 182, 566, 595, 634, 748, 1059, 1744
LweI GCATC 9 cut(s) 508, 541, 616, 1043, 1102, 1324, 1368, 1495, 1826
MabI ACCWGGT 1 cut(s) 1023
MaeI CTAG 1 cut(s) 498
MaeII ACGT 3 cut(s) 69, 186, 1225
MaeIII GTNAC 4 cut(s) 137, 446, 659, 1700
MalI GATC 5 cut(s) 113, 528, 1216, 1278, 1576
MbiI CCGCTC 1 cut(s) 1653
MboI GATC 5 cut(s) 111, 526, 1214, 1276, 1574
MfeI CAATTG 1 cut(s) 1638
MflI RGATCY 3 cut(s) 1214, 1276, 1574
MhlI GDGCHC 5 cut(s) 20, 728, 1494, 1828, 1857
MlsI TGGCCA 2 cut(s) 1445, 1515
MluCI AATT 8 cut(s) 164, 301, 788, 797, 1344, 1638, 1727, 1870
MluNI TGGCCA 2 cut(s) 1445, 1515
MlyI GAGTC 6 cut(s) 857, 980, 1361, 1373, 1382, 1780
MmeI TCCRAC 3 cut(s) 82, 1246, 1356
Mox20I TGGCCA 2 cut(s) 1445, 1515
MscI TGGCCA 2 cut(s) 1445, 1515
MseI TTAA 6 cut(s) 213, 332, 825, 1118, 1170, 1873
MslI CAYNNNNRTG 1 cut(s) 1427
Msp20I TGGCCA 2 cut(s) 1445, 1515
MspI CCGG 4 cut(s) 95, 117, 327, 1038
MspR9I CCNGG 5 cut(s) 95, 900, 1025, 1039, 1579
MunI CAATTG 1 cut(s) 1638
Mva1269I GAATGC 1 cut(s) 1838
MvaI CCWGG 3 cut(s) 900, 1025, 1579
MvnI CGCG 2 cut(s) 871, 1772
MwoI GCNNNNNNNGC 5 cut(s) 312, 687, 996, 1250, 1823
NciI CCSGG 2 cut(s) 95, 1039
NcoI CCATGG 1 cut(s) 1446
NdeII GATC 5 cut(s) 111, 526, 1214, 1276, 1574
NlaIII CATG 8 cut(s) 89, 685, 1005, 1240, 1426, 1450, 1520, 1852
NlaIV GGNNCC 6 cut(s) 133, 711, 727, 1007, 1452, 1576
NmuCI GTSAC 2 cut(s) 137, 659
PciSI GCTCTTC 2 cut(s) 947, 1660
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 1838
PfeI GAWTC 2 cut(s) 11, 491
PflMI CCANNNNNTGG 1 cut(s) 1015
PkrI GCNGC 8 cut(s) 172, 414, 556, 610, 649, 763, 1074, 1734
PleI GAGTC 6 cut(s) 856, 979, 1360, 1373, 1381, 1780
PpsI GAGTC 6 cut(s) 856, 979, 1360, 1373, 1381, 1780
Psp124BI GAGCTC 3 cut(s) 20, 1828, 1857
Psp6I CCWGG 3 cut(s) 898, 1023, 1577
PspGI CCWGG 3 cut(s) 898, 1023, 1577
PspN4I GGNNCC 6 cut(s) 133, 711, 727, 1007, 1452, 1576
PspPI GGNCC 5 cut(s) 82, 92, 200, 1314, 1859
PstI CTGCAG 2 cut(s) 766, 1324
PstNI CAGNNNCTG 1 cut(s) 1144
PsuI RGATCY 3 cut(s) 1214, 1276, 1574
RsaI GTAC 4 cut(s) 181, 908, 1395, 1452
RsaNI GTAC 4 cut(s) 180, 907, 1394, 1451
RseI CAYNNNNRTG 1 cut(s) 1427
SacI GAGCTC 3 cut(s) 20, 1828, 1857
SalI GTCGAC 2 cut(s) 752, 1052
SapI GCTCTTC 2 cut(s) 947, 1660
SaqAI TTAA 6 cut(s) 213, 332, 825, 1118, 1170, 1873
SatI GCNGC 8 cut(s) 171, 413, 555, 609, 648, 762, 1073, 1733
Sau3AI GATC 5 cut(s) 111, 526, 1214, 1276, 1574
Sau96I GGNCC 5 cut(s) 82, 92, 200, 1314, 1859
ScaI AGTACT 2 cut(s) 908, 1395
SchI GAGTC 6 cut(s) 857, 980, 1361, 1373, 1382, 1780
ScrFI CCNGG 5 cut(s) 95, 900, 1025, 1039, 1579
SduI GDGCHC 5 cut(s) 20, 728, 1494, 1828, 1857
SexAI ACCWGGT 1 cut(s) 1023
SfaNI GCATC 9 cut(s) 508, 541, 616, 1043, 1102, 1324, 1368, 1495, 1826
SfcI CTRYAG 5 cut(s) 741, 762, 915, 1077, 1320
SinI GGWCC 3 cut(s) 82, 1314, 1859
SmiMI CAYNNNNRTG 1 cut(s) 1427
SmlI CTYRAG 2 cut(s) 977, 1413
SmoI CTYRAG 2 cut(s) 977, 1413
Sse9I AATT 8 cut(s) 164, 301, 788, 797, 1344, 1638, 1727, 1870
SsiI CCGC 5 cut(s) 59, 413, 746, 1098, 1651
SspMI CTAG 1 cut(s) 498
SstI GAGCTC 3 cut(s) 20, 1828, 1857
StyD4I CCNGG 5 cut(s) 93, 898, 1023, 1037, 1577
StyI CCWWGG 2 cut(s) 636, 1446
TaaI ACNGT 3 cut(s) 211, 742, 906
TaiI ACGT 3 cut(s) 72, 189, 1228
TaqI TCGA 6 cut(s) 14, 289, 753, 922, 1053, 1355
TaqII GACCGA 1 cut(s) 1847
TasI AATT 8 cut(s) 164, 301, 788, 797, 1344, 1638, 1727, 1870
TatI WGTACW 3 cut(s) 179, 906, 1393
TauI GCSGC 1 cut(s) 415
TfiI GAWTC 2 cut(s) 11, 491
Tru1I TTAA 6 cut(s) 213, 332, 825, 1118, 1170, 1873
Tru9I TTAA 6 cut(s) 213, 332, 825, 1118, 1170, 1873
TscAI CASTG 3 cut(s) 149, 346, 1324
TseFI GTSAC 2 cut(s) 137, 659
TseI GCWGC 7 cut(s) 170, 554, 608, 647, 761, 1072, 1732
Tsp45I GTSAC 2 cut(s) 137, 659
TspGWI ACGGA 2 cut(s) 144, 176
TspRI CASTG 3 cut(s) 149, 346, 1324
Van91I CCANNNNNTGG 1 cut(s) 1015
VpaK11BI GGWCC 3 cut(s) 82, 1314, 1859
XagI CCTNNNNNAGG 1 cut(s) 719
XapI RAATTY 2 cut(s) 164, 797
XmiI GTMKAC 3 cut(s) 245, 753, 1053
XspI CTAG 1 cut(s) 498
ZrmI AGTACT 2 cut(s) 908, 1395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.