Rorug02G0500600

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000989
Physical Location & Seq
Forward (+)
63318843 .. 63319394
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0500600.1

Sequence Viewer

Length: 552 bp
ATGAAGTTTGATGGGGAAACGGTTCTTTTGGATTTGACAGGGTCGTTGGATCGCGGTGGTGGTTCGCAATGCAATGATGGAGGGGCCTCGCTTCTGAGAGGCTGGAAAGTTGGAATCGGGGTTCTGCGTTCTGTTGGTTTGCCGCCGGCTTGGGCTATGCGAAGTAGTGGCTTTGATGGTGTTGCTGGGCCGTTAGAAGTGGTCGCCGGCGTCGATCGGTCGTCTCAGGATTTCATGGAGTCAGTCGCTTTCCAATGGAGCAGGATAGATGGTGTGGGTGGTGGGGGCCACGGTGGAGGCTCCTCTCTGACTGCGGTGGTGTTAGAGGTGGAGATGAGGACGTGTAAAGCTGCGGTGACTGGTTGGAAGGTCTACCAATTCCTGTTGAACCCTTGCTTGGTTGGGCTAGGGTTTCTCTTGGATTCTGTTGATCTGGGGTGGGCTTGGCTGCTTTTGGCCCACTGGAAATTTAGTTTTATGTTTGTTACTTTAGTTTTGGTCATTATTCCTTTATTCTATAAAAGGAATATGACTAGTTCTATTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

183

Amino Acids

19.63

Weight (kDa)

6.07

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 54
AciI CCGC 4 cut(s) 54, 143, 314, 353
AclWI GGATC 1 cut(s) 57
AcsI RAATTY 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 210
AfiI CCNNNNNNNGG 1 cut(s) 397
AflIII ACRYGT 1 cut(s) 341
AgsI TTSAA 1 cut(s) 388
AhlI ACTAGT 1 cut(s) 533
AjiI CACGTC 1 cut(s) 342
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 1 cut(s) 350
AluI AGCT 1 cut(s) 350
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 57
AoxI GGCC 4 cut(s) 84, 188, 286, 456
ApeKI GCWGC 2 cut(s) 350, 448
ApoI RAATTY 1 cut(s) 467
Asp700I GAANNNNTTC 1 cut(s) 21
AspS9I GGNCC 4 cut(s) 84, 188, 286, 457
AsuHPI GGTGA 1 cut(s) 367
BbvI GCAGC 2 cut(s) 337, 435
BccI CCATC 4 cut(s) 5, 71, 170, 263
BceAI ACGGC 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 228
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 2 cut(s) 407, 534
BisI GCNGC 3 cut(s) 143, 351, 449
BlsI GCNGC 3 cut(s) 144, 352, 450
BmgBI CACGTC 1 cut(s) 342
BmgT120I GGNCC 4 cut(s) 84, 188, 286, 457
BmiI GGNNCC 3 cut(s) 85, 287, 301
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse118I RCCGGY 2 cut(s) 145, 206
Bse1I ACTGG 2 cut(s) 364, 467
Bse3DI GCAATG 2 cut(s) 74, 79
BseDI CCNNGG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMI GCAATG 2 cut(s) 74, 79
BseMII CTCAG 2 cut(s) 86, 239
BseNI ACTGG 2 cut(s) 364, 467
BseRI GAGGAG 1 cut(s) 292
BseXI GCAGC 2 cut(s) 337, 435
BseYI CCCAGC 1 cut(s) 185
Bsh1236I CGCG 1 cut(s) 54
Bsh1285I CGRYCG 2 cut(s) 217, 221
BshFI GGCC 4 cut(s) 86, 190, 288, 458
BsiEI CGRYCG 2 cut(s) 217, 221
BsiSI CCGG 2 cut(s) 146, 207
BslI CCNNNNNNNGG 1 cut(s) 397
BsmAI GTCTC 1 cut(s) 228
BsmBI CGTCTC 1 cut(s) 228
BsnI GGCC 4 cut(s) 86, 190, 288, 458
Bsp143I GATC 3 cut(s) 49, 214, 430
BspACI CCGC 4 cut(s) 54, 143, 314, 353
BspANI GGCC 4 cut(s) 86, 190, 288, 458
BspCNI CTCAG 2 cut(s) 87, 238
BspFNI CGCG 1 cut(s) 54
BspLI GGNNCC 3 cut(s) 85, 287, 301
BspPI GGATC 1 cut(s) 57
BsrDI GCAATG 2 cut(s) 74, 79
BsrFI RCCGGY 2 cut(s) 145, 206
BsrI ACTGG 2 cut(s) 364, 467
BssAI RCCGGY 2 cut(s) 145, 206
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 3 cut(s) 49, 214, 430
BssNI GRCGYC 1 cut(s) 210
Bst4CI ACNGT 2 cut(s) 22, 293
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 147, 208
BstDEI CTNAG 2 cut(s) 95, 225
BstDSI CCRYGG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 3 cut(s) 52, 217, 433
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 3 cut(s) 49, 214, 430
BstMCI CGRYCG 2 cut(s) 217, 221
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 337, 435
BsuRI GGCC 4 cut(s) 86, 190, 288, 458
BtgI CCRYGG 1 cut(s) 289
BtrI CACGTC 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 2 cut(s) 147, 208
Cfr10I RCCGGY 2 cut(s) 145, 206
Cfr13I GGNCC 4 cut(s) 84, 188, 286, 457
CseI GACGC 1 cut(s) 199
CviAII CATG 1 cut(s) 235
DdeI CTNAG 2 cut(s) 95, 225
DpnI GATC 3 cut(s) 51, 216, 432
DpnII GATC 3 cut(s) 49, 214, 430
EcoO109I RGGNCCY 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 1 cut(s) 238
FaiI YATR 5 cut(s) 158, 236, 479, 519, 530
FatI CATG 1 cut(s) 234
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 3 cut(s) 143, 351, 449
Fsp4HI GCNGC 3 cut(s) 143, 351, 449
FspBI CTAG 2 cut(s) 407, 534
GluI GCNGC 3 cut(s) 143, 351, 449
GsaI CCCAGC 1 cut(s) 189
HaeIII GGCC 4 cut(s) 86, 190, 288, 458
HapII CCGG 2 cut(s) 146, 207
HgaI GACGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 1 cut(s) 238
HinfI GANTC 3 cut(s) 114, 239, 422
HpaII CCGG 2 cut(s) 146, 207
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 96, 309
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 2 cut(s) 22, 293
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 1 cut(s) 72
HpyF3I CTNAG 2 cut(s) 95, 225
HpySE526I ACGT 1 cut(s) 341
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 1 cut(s) 238
KroI GCCGGC 2 cut(s) 145, 206
KroNI GCCGGC 2 cut(s) 147, 208
Kzo9I GATC 3 cut(s) 49, 214, 430
LmnI GCTCC 2 cut(s) 258, 305
Lsp1109I GCAGC 2 cut(s) 337, 435
MaeI CTAG 2 cut(s) 407, 534
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 2 cut(s) 355, 484
MalI GATC 3 cut(s) 51, 216, 432
MboI GATC 3 cut(s) 49, 214, 430
MluCI AATT 2 cut(s) 377, 467
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 3 cut(s) 27, 91, 344
MnlI CCTC 7 cut(s) 74, 92, 97, 290, 313, 319, 330
MreI CGCCGGCG 1 cut(s) 206
MroNI GCCGGC 2 cut(s) 145, 206
MroXI GAANNNNTTC 1 cut(s) 21
MspI CCGG 2 cut(s) 146, 207
MvnI CGCG 1 cut(s) 54
NaeI GCCGGC 2 cut(s) 147, 208
NdeII GATC 3 cut(s) 49, 214, 430
NgoMIV GCCGGC 2 cut(s) 145, 206
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 3 cut(s) 85, 287, 301
NmuCI GTSAC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdiI GCCGGC 2 cut(s) 147, 208
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 2 cut(s) 114, 422
PflFI GACNNNGTC 1 cut(s) 40
PkrI GCNGC 3 cut(s) 144, 352, 450
Ple19I CGATCG 1 cut(s) 217
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 185
PspN4I GGNNCC 3 cut(s) 85, 287, 301
PspPI GGNCC 4 cut(s) 84, 188, 286, 457
PsyI GACNNNGTC 1 cut(s) 40
PvuI CGATCG 1 cut(s) 217
SatI GCNGC 3 cut(s) 143, 351, 449
Sau3AI GATC 3 cut(s) 49, 214, 430
Sau96I GGNCC 4 cut(s) 84, 188, 286, 457
SchI GAGTC 1 cut(s) 248
SetI ASST 4 cut(s) 330, 344, 352, 372
SgrAI CRCCGGYG 1 cut(s) 206
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 2 cut(s) 377, 467
SsiI CCGC 4 cut(s) 54, 143, 314, 353
SspMI CTAG 2 cut(s) 407, 534
TaaI ACNGT 2 cut(s) 22, 293
TaiI ACGT 1 cut(s) 344
TaqI TCGA 1 cut(s) 213
TaqII GACCGA 1 cut(s) 207
TasI AATT 2 cut(s) 377, 467
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 2 cut(s) 114, 422
TscAI CASTG 1 cut(s) 467
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 2 cut(s) 350, 448
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 2 cut(s) 17, 223
TspRI CASTG 1 cut(s) 467
Tth111I GACNNNGTC 1 cut(s) 40
XapI RAATTY 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 21
XspI CTAG 2 cut(s) 407, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.