MD09G1085300.v1.1

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000989
Physical Location & Seq
Reverse (-)
6096517 .. 6100400
3884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1085300.v1.1.491

Sequence Viewer

Length: 1827 bp
ATGGTGGTGGAGGAGAGCAACAAGGTTGAGGATACTCTGGTGGTCGGGAAGCAACGCGTTCGGGCCGTCGCTGGCCAGATCTATGACTCCAAGAAAGGAGTTTCCTGCCATCAGTGCCGTCAGAAGATGACGGAAAAGTTGACAGCTTTGGCGGCCTCCTGTAAGAACGTCAAAGCGAAAGGCATTTGCCCGATTCAGTTTTGCCATAAGTGTCTGTGGAACAGGTATGGATTGAAAGCTGAACAAGTGGAACTCGAGGAGGATTGGAAATGTCCGAAATGTCAAGGCAATTGCAATTGCAGTATATGCTTGAAGAAAAACGGTTTCCAACCCACTGGTCTTCTCGTGTACGAAGCCAAGGCGAGAGGGTTTCAATCTGTTTCTGATATGCTGACTATGTACAAGCCAAAAAAGCAAGGGGAGGAGGATGATAAAAGCAAGGATGGCTCGCCACAAAAGCGTGTTGCTTCAGACAAGGCAATTGTGGTTAGTTCACCAAGAAAACGAGGGAAGGAGAACACTCTCGATCGAAGCAAAGATATGAACTTGGATGCAGCTCTGAACTCTGGTGAGCAGGAAACTAAGAAAGCAAAGCGGGAAGAGTTGACGGAAGTTTGTAATGGCAGCAGAGATGGTGGTGCTGCTGTGAGTAATAAGGATGCCAAGCCCAAGGCCAAGGAAGTCAGAAGGCATCGGAAAGTAAAGGAATGCCCCGTGAATATTGAGAATCAAAAGGTTCAACTTGACCTTCCCCTGCCACAGGGCAATCCCTTGACATCTGTAGCTGGTGTTGAATTGGCTGCTGAGGATGTTGGAAATGCTCTGCAGTTTCTGAAATTTTGCGAAACTTTTGGAGAGTTTCTTAAAATCCAAAAATGCCAAGCTGAATCTGTACTTAGAGAACTATCACGTCGAGGAAGTGGGCGAAGGGGACGGCCTGGACAATACTCCTCAGTGATTCGATTTCATAACTATATGCTCTCCTTCATACAGAAGGGTTCCTTAGAGGAGTCTTCTTCTTTAGATCAATCAAGCCACAATAAGTCCTGGTTCCAAGATTTGGGGGAACTTATCTCTGCATCCGGTGTCTCTAAGGTCGATCCTGTATTAAAGGAACTGTCCCCAGAGTGTTTTAGCAAAGGCGGGGATGGATATGATATGTTGAACATCACTCAAAAGCTCAGGATTTTGAATTTCCTTTGTGATGAGGCTCTTGACACCGATATGCTAAGTGGATGGATAGAGGAACAAAATGAAAAATTGGTCCAACGAAAAAGGGACGCCAAAGAAAAAGTTGCTAAAGCAAAGGATAAGGAGAGAGATCTCAAAAAGAAGTTGCAGGAGGAGATGGCAAAGAGGATAATTTCAAACAATGGTTATCCTGTCCGAATTTCAGAACGTAACTCTATTGTTTCACAAATCACAAGCAAGGCAGCTAAAGCTCACACTGAGGTGGTTGAAGCAATGGCCATGTTACCTAAAAGCAATGAAATATGTGAGGCTGTTAGAATAGAGGCTTATGTTTTGGATGTTCATGGCCGTGCATTTTGGAAACTGAACGGTGATTTTGGTGAAGATATTTTGCTTCAAGATATGGGAACGTGGGATGGAGCTCCTTCTGTTGAAACCTGGTTCGTTTATGGTGCTGAAATGAAAGAGCCAATCGACAAATACCTCTCTTTTCTGAGTCTAAAACAAGAACGGGCCAGGGGCCAGAGGCCATTCAAAACTCGGTTTGCAAGCAATGAAGAAGACTTGCAGGTTCCTCAGACTCCTGAAAGCAATGAAGAAAACCCTCCGGTTTCCGACACCTTCTCAAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

609

Amino Acids

68.27

Weight (kDa)

8.66

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 28 - 131 2.3e-31 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1750
AccB7I CCANNNNNTGG 1 cut(s) 1060
AccII CGCG 1 cut(s) 57
AciI CCGC 3 cut(s) 152, 595, 1143
AclWI GGATC 1 cut(s) 1094
AcoI YGGCCR 3 cut(s) 73, 1467, 1537
AcsI RAATTY 3 cut(s) 836, 1192, 1389
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 1281
AfaI GTAC 3 cut(s) 350, 401, 894
AfiI CCNNNNNNNGG 2 cut(s) 760, 1060
AflIII ACRYGT 1 cut(s) 55
AjiI CACGTC 1 cut(s) 911
AjnI CCWGG 4 cut(s) 937, 1046, 1628, 1706
AleI CACNNNNGTG 1 cut(s) 1451
AluBI AGCT 9 cut(s) 146, 239, 557, 785, 884, 1180, 1436, 1442, 1613
AluI AGCT 9 cut(s) 146, 239, 557, 785, 884, 1180, 1436, 1442, 1613
Alw21I GWGCWC 1 cut(s) 1615
Alw26I GTCTC 1 cut(s) 1093
AlwI GGATC 1 cut(s) 1094
AlwNI CAGNNNCTG 1 cut(s) 832
Ama87I CYCGRG 1 cut(s) 254
ApeKI GCWGC 5 cut(s) 554, 624, 641, 800, 1433
ApoI RAATTY 3 cut(s) 836, 1192, 1389
AspS9I GGNCC 4 cut(s) 63, 1264, 1704, 1711
AsuHPI GGTGA 4 cut(s) 486, 581, 1574, 1583
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 1264
BaeI ACNNNNGTAYC 2 cut(s) 24, 57
BalI TGGCCA 2 cut(s) 75, 1469
BanII GRGCYC 1 cut(s) 1615
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 3 cut(s) 332, 1005, 1758
Bbv12I GWGCWC 1 cut(s) 1615
BbvCI CCTCAGC 1 cut(s) 804
BbvI GCAGC 5 cut(s) 566, 628, 636, 787, 1445
BccI CCATC 7 cut(s) 117, 437, 626, 1142, 1230, 1342, 1601
BceAI ACGGC 4 cut(s) 50, 102, 950, 1524
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciT130I CCWGG 4 cut(s) 939, 1048, 1630, 1708
BciVI GTATCC 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 1093
BfmI CTRYAG 2 cut(s) 780, 824
BfuAI ACCTGC 1 cut(s) 1750
BfuI GTATCC 1 cut(s) 25
BglII AGATCT 2 cut(s) 78, 1321
BisI GCNGC 6 cut(s) 153, 555, 625, 642, 801, 1434
BlsI GCNGC 6 cut(s) 154, 556, 626, 643, 802, 1435
Bme1390I CCNGG 4 cut(s) 939, 1048, 1630, 1708
Bme18I GGWCC 1 cut(s) 1264
BmeT110I CYCGRG 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 911
BmgT120I GGNCC 4 cut(s) 63, 1264, 1704, 1711
BmiI GGNNCC 4 cut(s) 1000, 1052, 1712, 1764
BmrFI CCNGG 4 cut(s) 939, 1048, 1630, 1708
BmsI GCATC 4 cut(s) 541, 649, 700, 1088
BpiI GAAGAC 3 cut(s) 332, 1005, 1758
Bpu10I CCTNAGC 2 cut(s) 804, 1181
BsaHI GRCGYC 1 cut(s) 1281
BsaJI CCNNGG 4 cut(s) 357, 669, 675, 1707
BsaWI WCCGGW 2 cut(s) 1082, 1798
Bsc4I CCNNNNNNNGG 2 cut(s) 760, 1060
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 4 cut(s) 1470, 1492, 1750, 1789
BseBI CCWGG 4 cut(s) 939, 1048, 1630, 1708
BseDI CCNNGG 4 cut(s) 357, 669, 675, 1707
BseLI CCNNNNNNNGG 2 cut(s) 760, 1060
BseMI GCAATG 4 cut(s) 1470, 1492, 1750, 1789
BseMII CTCAG 6 cut(s) 795, 966, 1195, 1440, 1676, 1781
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 6 cut(s) 26, 272, 437, 940, 1022, 1358
BseXI GCAGC 5 cut(s) 566, 628, 636, 787, 1445
Bsh1236I CGCG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 529
BsiEI CGRYCG 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 1615
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 2 cut(s) 1083, 1799
BslFI GGGAC 3 cut(s) 945, 1105, 1292
BslI CCNNNNNNNGG 2 cut(s) 760, 1060
BsmAI GTCTC 1 cut(s) 1093
BsmFI GGGAC 3 cut(s) 945, 1105, 1292
BsmI GAATGC 1 cut(s) 713
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 1615
Bsp1407I TGTACA 1 cut(s) 399
Bsp143I GATC 5 cut(s) 78, 526, 1024, 1099, 1321
BspACI CCGC 3 cut(s) 152, 595, 1143
BspCNI CTCAG 6 cut(s) 796, 965, 1194, 1441, 1677, 1780
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 4 cut(s) 1000, 1052, 1712, 1764
BspMAI CTGCAG 1 cut(s) 828
BspMI ACCTGC 1 cut(s) 1750
BspPI GGATC 1 cut(s) 1094
BsrDI GCAATG 4 cut(s) 1470, 1492, 1750, 1789
BsrGI TGTACA 1 cut(s) 399
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 4 cut(s) 357, 669, 675, 1707
BssMI GATC 5 cut(s) 78, 526, 1024, 1099, 1321
BssNI GRCGYC 1 cut(s) 1281
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 3 cut(s) 357, 669, 675
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 4 cut(s) 939, 1048, 1630, 1708
Bst4CI ACNGT 3 cut(s) 323, 1119, 1562
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 1281
BstAPI GCANNNNNTGC 1 cut(s) 306
BstAUI TGTACA 1 cut(s) 399
BstC8I GCNNGC 3 cut(s) 73, 449, 1741
BstENI CCTNNNNNAGG 1 cut(s) 758
BstFNI CGCG 1 cut(s) 57
BstKTI GATC 5 cut(s) 81, 529, 1027, 1102, 1324
BstMAI GTCTC 1 cut(s) 1093
BstMBI GATC 5 cut(s) 78, 526, 1024, 1099, 1321
BstMCI CGRYCG 1 cut(s) 529
BstMWI GCNNNNNNNGC 7 cut(s) 114, 152, 306, 412, 444, 457, 1439
BstNI CCWGG 4 cut(s) 939, 1048, 1630, 1708
BstSCI CCNGG 4 cut(s) 937, 1046, 1628, 1706
BstSFI CTRYAG 2 cut(s) 780, 824
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 5 cut(s) 566, 628, 636, 787, 1445
BstV2I GAAGAC 3 cut(s) 332, 1005, 1758
BstX2I RGATCY 2 cut(s) 78, 1321
BstXI CCANNNNNNTGG 1 cut(s) 335
BstYI RGATCY 2 cut(s) 78, 1321
BsuI GTATCC 1 cut(s) 25
BtrI CACGTC 1 cut(s) 911
BtsIMutI CAGTG 4 cut(s) 119, 333, 960, 1446
BveI ACCTGC 1 cut(s) 1750
Cac8I GCNNGC 3 cut(s) 73, 449, 1741
CaiI CAGNNNCTG 1 cut(s) 832
Cfr13I GGNCC 4 cut(s) 63, 1264, 1704, 1711
CseI GACGC 1 cut(s) 1289
CsiI ACCWGGT 1 cut(s) 1628
Csp6I GTAC 3 cut(s) 349, 400, 893
CviAII CATG 2 cut(s) 1471, 1535
CviQI GTAC 3 cut(s) 349, 400, 893
DpnI GATC 5 cut(s) 80, 528, 1026, 1101, 1323
DpnII GATC 5 cut(s) 78, 526, 1024, 1099, 1321
EaeI YGGCCR 3 cut(s) 73, 1467, 1537
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 1613
Eco130I CCWWGG 3 cut(s) 357, 669, 675
Eco24I GRGCYC 1 cut(s) 1615
Eco47I GGWCC 1 cut(s) 1264
Eco53kI GAGCTC 1 cut(s) 1613
Eco57I CTGAAG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 1613
EcoNI CCTNNNNNAGG 1 cut(s) 758
EcoRII CCWGG 4 cut(s) 937, 1046, 1628, 1706
EcoT14I CCWWGG 3 cut(s) 357, 669, 675
EcoT38I GRGCYC 1 cut(s) 1615
ErhI CCWWGG 3 cut(s) 357, 669, 675
FaeI CATG 2 cut(s) 1474, 1538
FalI AAGNNNNNCTT 2 cut(s) 986, 1018
FaqI GGGAC 3 cut(s) 945, 1105, 1292
FatI CATG 2 cut(s) 1470, 1534
FauI CCCGC 2 cut(s) 588, 1136
Fnu4HI GCNGC 6 cut(s) 153, 555, 625, 642, 801, 1434
FriOI GRGCYC 1 cut(s) 1615
Fsp4HI GCNGC 6 cut(s) 153, 555, 625, 642, 801, 1434
GluI GCNGC 6 cut(s) 153, 555, 625, 642, 801, 1434
HapII CCGG 2 cut(s) 1083, 1799
HgaI GACGC 1 cut(s) 1289
Hin1I GRCGYC 1 cut(s) 1281
Hin1II CATG 2 cut(s) 1474, 1538
HincII GTYRAC 2 cut(s) 141, 606
HindII GTYRAC 2 cut(s) 141, 606
HinfI GANTC 8 cut(s) 86, 193, 727, 887, 958, 1010, 1687, 1771
HpaII CCGG 2 cut(s) 1083, 1799
HphI GGTGA 4 cut(s) 486, 581, 1574, 1583
Hpy166II GTNNAC 4 cut(s) 141, 349, 494, 606
Hpy188III TCNNGA 6 cut(s) 46, 524, 1183, 1214, 1589, 1775
Hpy8I GTNNAC 4 cut(s) 141, 349, 494, 606
Hpy99I CGWCG 2 cut(s) 71, 915
HpyAV CCTTC 8 cut(s) 505, 681, 758, 921, 988, 994, 1626, 1822
HpyCH4III ACNGT 3 cut(s) 323, 1119, 1562
HpyCH4IV ACGT 4 cut(s) 168, 910, 1399, 1601
HpyCH4V TGCA 9 cut(s) 294, 300, 554, 826, 1079, 1339, 1544, 1739, 1759
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 152, 306, 412, 444, 457, 1439
HpySE526I ACGT 4 cut(s) 168, 910, 1399, 1601
Hsp92I GRCGYC 1 cut(s) 1281
Hsp92II CATG 2 cut(s) 1474, 1538
Kzo9I GATC 5 cut(s) 78, 526, 1024, 1099, 1321
LmnI GCTCC 2 cut(s) 1610, 1618
Lsp1109I GCAGC 5 cut(s) 566, 628, 636, 787, 1445
LweI GCATC 4 cut(s) 541, 649, 700, 1088
MabI ACCWGGT 1 cut(s) 1628
MaeII ACGT 4 cut(s) 168, 910, 1399, 1601
MaeIII GTNAC 2 cut(s) 1400, 1473
MalI GATC 5 cut(s) 80, 528, 1026, 1101, 1323
MboI GATC 5 cut(s) 78, 526, 1024, 1099, 1321
MfeI CAATTG 3 cut(s) 289, 295, 480
MflI RGATCY 2 cut(s) 78, 1321
MhlI GDGCHC 1 cut(s) 1615
MlsI TGGCCA 2 cut(s) 75, 1469
MluCI AATT 9 cut(s) 289, 295, 480, 794, 836, 1192, 1259, 1362, 1389
MluI ACGCGT 1 cut(s) 55
MluNI TGGCCA 2 cut(s) 75, 1469
MlyI GAGTC 4 cut(s) 80, 1019, 1696, 1765
MmeI TCCRAC 3 cut(s) 352, 793, 1291
Mox20I TGGCCA 2 cut(s) 75, 1469
MscI TGGCCA 2 cut(s) 75, 1469
MseI TTAA 2 cut(s) 864, 1109
MslI CAYNNNNRTG 3 cut(s) 1223, 1451, 1539
Msp20I TGGCCA 2 cut(s) 75, 1469
MspI CCGG 2 cut(s) 1083, 1799
MspR9I CCNGG 4 cut(s) 939, 1048, 1630, 1708
MunI CAATTG 3 cut(s) 289, 295, 480
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 4 cut(s) 939, 1048, 1630, 1708
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 7 cut(s) 114, 152, 306, 412, 444, 457, 1439
NdeII GATC 5 cut(s) 78, 526, 1024, 1099, 1321
NlaIII CATG 2 cut(s) 1474, 1538
NlaIV GGNNCC 4 cut(s) 1000, 1052, 1712, 1764
OliI CACNNNNGTG 1 cut(s) 1451
PaeR7I CTCGAG 1 cut(s) 254
PctI GAATGC 1 cut(s) 713
PfeI GAWTC 4 cut(s) 193, 727, 887, 958
PflMI CCANNNNNTGG 1 cut(s) 1060
PkrI GCNGC 6 cut(s) 154, 556, 626, 643, 802, 1435
Ple19I CGATCG 1 cut(s) 529
PleI GAGTC 4 cut(s) 80, 1018, 1695, 1765
PpsI GAGTC 4 cut(s) 80, 1018, 1695, 1765
Psp124BI GAGCTC 1 cut(s) 1615
Psp6I CCWGG 4 cut(s) 937, 1046, 1628, 1706
PspGI CCWGG 4 cut(s) 937, 1046, 1628, 1706
PspN4I GGNNCC 4 cut(s) 1000, 1052, 1712, 1764
PspPI GGNCC 4 cut(s) 63, 1264, 1704, 1711
PspXI VCTCGAGB 1 cut(s) 254
PstI CTGCAG 1 cut(s) 828
PstNI CAGNNNCTG 1 cut(s) 832
PsuI RGATCY 2 cut(s) 78, 1321
PvuI CGATCG 1 cut(s) 529
RsaI GTAC 3 cut(s) 350, 401, 894
RsaNI GTAC 3 cut(s) 349, 400, 893
RseI CAYNNNNRTG 3 cut(s) 1223, 1451, 1539
SacI GAGCTC 1 cut(s) 1615
SaqAI TTAA 2 cut(s) 864, 1109
SatI GCNGC 6 cut(s) 153, 555, 625, 642, 801, 1434
Sau3AI GATC 5 cut(s) 78, 526, 1024, 1099, 1321
Sau96I GGNCC 4 cut(s) 63, 1264, 1704, 1711
SchI GAGTC 4 cut(s) 80, 1019, 1696, 1765
ScrFI CCNGG 4 cut(s) 939, 1048, 1630, 1708
SduI GDGCHC 1 cut(s) 1615
SexAI ACCWGGT 1 cut(s) 1628
SfaNI GCATC 4 cut(s) 541, 649, 700, 1088
SfcI CTRYAG 2 cut(s) 780, 824
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 1264
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 3 cut(s) 1223, 1451, 1539
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 9 cut(s) 289, 295, 480, 794, 836, 1192, 1259, 1362, 1389
SsiI CCGC 3 cut(s) 152, 595, 1143
SspI AATATT 1 cut(s) 721
SstI GAGCTC 1 cut(s) 1615
StyD4I CCNGG 4 cut(s) 937, 1046, 1628, 1706
StyI CCWWGG 3 cut(s) 357, 669, 675
TaaI ACNGT 3 cut(s) 323, 1119, 1562
TaiI ACGT 4 cut(s) 171, 913, 1402, 1604
TaqI TCGA 7 cut(s) 255, 525, 529, 913, 961, 1098, 1665
TasI AATT 9 cut(s) 289, 295, 480, 794, 836, 1192, 1259, 1362, 1389
TatI WGTACW 2 cut(s) 399, 892
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 4 cut(s) 193, 727, 887, 958
Tru1I TTAA 2 cut(s) 864, 1109
Tru9I TTAA 2 cut(s) 864, 1109
TscAI CASTG 4 cut(s) 119, 340, 960, 1453
TseI GCWGC 5 cut(s) 554, 624, 641, 800, 1433
TspDTI ATGAA 9 cut(s) 557, 956, 976, 1269, 1503, 1523, 1667, 1761, 1800
TspGWI ACGGA 2 cut(s) 146, 623
TspRI CASTG 4 cut(s) 119, 340, 960, 1453
Van91I CCANNNNNTGG 1 cut(s) 1060
VpaK11BI GGWCC 1 cut(s) 1264
XagI CCTNNNNNAGG 1 cut(s) 758
XapI RAATTY 3 cut(s) 836, 1192, 1389
XhoI CTCGAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.