Rh2BG579200

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000989
Physical Location & Seq
Forward (+)
79657892 .. 79661373
3482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG579200.1

Sequence Viewer

Length: 1536 bp
ATGTTGACTGCGAGAGGACCGGAGAATTTTGGTATTGAGAGGAAGGTTGCGGAGAAGGTGGCTGTCGATGGAAAGGAGAACTCTGTTGATCAAAAGATTGAGGCCAGCCTGAATTCAACACAAAATCCTGAGGAGGGACAAGTCAAGAAAACAAAGAGGAAAGGATTGAAGGAAATCCGTAATGGCAGCAGGGATGATCCTAAAGTCTCCGAGGATGGCAGCAGGGATGATCCTAAAGTCTCCGAGAATGGCAGCAGGGATGATCCTAAAGTCCCCGAGGATGGCAGCAGGGATGATCCTAAAGTCTCCAAGGATGTTGCTGTAAAAGAAGTGAAATCAAGAAAGAGAAAAAATAAAGATCGAAGAGAAGAAGCAGATGATGCGAAACTGCAAGGTGAAGAAGTTCAAAATGTTGTGAACAACAATGATGTCAAGAGGAAGAAAAAGGCCAAGGACAAGGTCAAGAGTAGCAAAGAGACTTGCAGAGTTGAGGAATGCACCAAGAATATTCTGAACAAAAAAGTTGAGCCTGAGCTTCCTTTGCCTGAGGGCATTCCCTTAACAAGTGTCTTGGGAATTGAACTGCCTCCTGAGGATGCTGGAAATGCTCTGCAATTTATGGAATTTTGTTCAGCTTTTGGAGAGGTTCTCAAATTCAAAAAAGCACAGGCTGAATCTATAATTGGTGAATTACTGCGCCAACGAAGTGGGCGAGCTGGCCGCCCTGGACAATACTCCTCACTTATTCGGTTTCACATGCAACTATTATCTCTACTACAGGAGGATATGGGAGAGGAGCCTTCATCTATAGATGAAATGAGCCACAAAAATTCATGGTTCCAAGATCTGGGTAAATGCATCTCTGAATCTGAGTCTGAACACCTATTGAAGGAACTGCCTACAGATTGTTTCAGTAGAGGTGGTGCTGGATATGATATCTTAGACCTCTCTCAGAAGCTCAGACTCTTAACTTACCTTTGTGATGAGGTGATTTGCACTTCTAAACTGAGGAGCTGGATAGAAGAACAGCATGAAAAGATTGTTGAAAGCGAAAAGGAAGCAAAAGGAAAAGTTAATGCAGCCAAAGATAAGGAAAAACTCCTCAAGCGGAAGTTGCAAGATGAGGTGGCGAAGCTTATTATTGCAAAAAATGGTGCTCCTCTGTCAATTTCAGAACATGAAGCTCTTGTTTCACAAATAAAAAGTGAAGCAGCTCAAGCTCATGCTGAGTTGCTTGAAGCAGAGGGCATAGTGCCTAAAAAGAAACATAGACGTGATGCTTTAAGAACGGAGCCTTATCGTGTGGATGTTGATGGCCGCATCTTTTTGAAATTAAAGGGTTACAACAATGGAGAAGATATCGTGCTTCAAGATTTGGGGGCCTGGGATGCAGTTGTATCTAAAGAAGAATGGTTTGTTTATGGTGCGGAGACAAAGGAAGCAATTGAAAATTACTGTTCTTCTTTAAGAAGAAAAAAGTCTAGTGGAACCATGTCGCAAACTGTTCCTCGTGAAAGTGATGAAGAAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

511

Amino Acids

57.4

Weight (kDa)

6.04

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 847
AciI CCGC 5 cut(s) 50, 721, 1108, 1318, 1427
AclWI GGATC 4 cut(s) 191, 224, 257, 290
AcoI YGGCCR 2 cut(s) 718, 1315
AcsI RAATTY 5 cut(s) 25, 112, 623, 653, 829
AfiI CCNNNNNNNGG 4 cut(s) 134, 281, 847, 889
AflIII ACRYGT 1 cut(s) 1530
AjiI CACGTC 1 cut(s) 1274
AjnI CCWGG 2 cut(s) 724, 1382
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 9 cut(s) 535, 635, 716, 958, 1014, 1135, 1184, 1214, 1220
AluI AGCT 9 cut(s) 535, 635, 716, 958, 1014, 1135, 1184, 1214, 1220
Alw21I GWGCWC 1 cut(s) 1159
Alw26I GTCTC 5 cut(s) 211, 244, 310, 470, 1424
AlwI GGATC 4 cut(s) 191, 224, 257, 290
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 5 cut(s) 102, 447, 718, 1315, 1380
ApeKI GCWGC 6 cut(s) 186, 219, 252, 285, 1079, 1211
ApoI RAATTY 5 cut(s) 25, 112, 623, 653, 829
Asp700I GAANNNNTTC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 699
AspS9I GGNCC 2 cut(s) 17, 1380
AsuHPI GGTGA 3 cut(s) 407, 698, 1000
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 17
AxyI CCTNAGG 3 cut(s) 129, 546, 591
BauI CACGAG 1 cut(s) 1509
Bbv12I GWGCWC 1 cut(s) 1159
BbvI GCAGC 6 cut(s) 198, 231, 264, 297, 1091, 1223
BccI CCATC 4 cut(s) 62, 209, 275, 1307
BciT130I CCWGG 2 cut(s) 726, 1384
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 5 cut(s) 211, 244, 310, 470, 1424
BfaI CTAG 1 cut(s) 1482
BfmI CTRYAG 3 cut(s) 776, 807, 900
BglII AGATCT 1 cut(s) 844
BisI GCNGC 8 cut(s) 187, 220, 253, 286, 721, 1080, 1212, 1318
BlsI GCNGC 8 cut(s) 188, 221, 254, 287, 722, 1081, 1213, 1319
Bme1390I CCNGG 2 cut(s) 726, 1384
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 275
BmgBI CACGTC 1 cut(s) 1274
BmgT120I GGNCC 2 cut(s) 17, 1380
BmiI GGNNCC 5 cut(s) 798, 839, 1293, 1381, 1489
BmrFI CCNGG 2 cut(s) 726, 1384
BmsI GCATC 6 cut(s) 370, 586, 867, 1267, 1329, 1378
BplI GAGNNNNNCTC 2 cut(s) 633, 665
Bpu10I CCTNAGC 1 cut(s) 531
BpuEI CTTGAG 2 cut(s) 1088, 1200
BsaJI CCNNGG 6 cut(s) 210, 276, 309, 450, 724, 1383
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 281, 847, 889
Bse21I CCTNAGG 3 cut(s) 129, 546, 591
BseBI CCWGG 2 cut(s) 726, 1384
BseDI CCNNGG 6 cut(s) 210, 276, 309, 450, 724, 1383
BseLI CCNNNNNNNGG 4 cut(s) 134, 281, 847, 889
BseMII CTCAG 9 cut(s) 120, 522, 537, 582, 861, 965, 973, 998, 1218
BseRI GAGGAG 6 cut(s) 146, 727, 809, 1024, 1091, 1149
BseXI GCAGC 6 cut(s) 198, 231, 264, 297, 1091, 1223
BshFI GGCC 5 cut(s) 104, 449, 720, 1317, 1382
BsiHKAI GWGCWC 1 cut(s) 1159
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 2 cut(s) 150, 257
BslI CCNNNNNNNGG 4 cut(s) 134, 281, 847, 889
BsmAI GTCTC 5 cut(s) 211, 244, 310, 470, 1424
BsmFI GGGAC 2 cut(s) 150, 257
BsmI GAATGC 2 cut(s) 500, 552
BsnI GGCC 5 cut(s) 104, 449, 720, 1317, 1382
BsoBI CYCGRG 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 1159
Bsp143I GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
BspACI CCGC 5 cut(s) 50, 721, 1108, 1318, 1427
BspANI GGCC 5 cut(s) 104, 449, 720, 1317, 1382
BspCNI CTCAG 9 cut(s) 121, 523, 538, 583, 862, 964, 972, 999, 1219
BspLI GGNNCC 5 cut(s) 798, 839, 1293, 1381, 1489
BspPI GGATC 4 cut(s) 191, 224, 257, 290
BssECI CCNNGG 6 cut(s) 210, 276, 309, 450, 724, 1383
BssMI GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
BssSI CACGAG 1 cut(s) 1509
BssT1I CCWWGG 2 cut(s) 309, 450
Bst2BI CACGAG 1 cut(s) 1509
Bst2UI CCWGG 2 cut(s) 726, 1384
Bst4CI ACNGT 2 cut(s) 1457, 1504
Bst6I CTCTTC 1 cut(s) 358
BstAPI GCANNNNNTGC 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 106, 714, 718
BstENI CCTNNNNNAGG 1 cut(s) 887
BstHHI GCGC 1 cut(s) 699
BstKTI GATC 7 cut(s) 91, 199, 232, 265, 298, 361, 847
BstMAI GTCTC 5 cut(s) 211, 244, 310, 470, 1424
BstMBI GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
BstMWI GCNNNNNNNGC 6 cut(s) 380, 541, 605, 1114, 1217, 1388
BstNI CCWGG 2 cut(s) 726, 1384
BstNSI RCATGY 2 cut(s) 760, 1534
BstSCI CCNGG 2 cut(s) 724, 1382
BstSFI CTRYAG 3 cut(s) 776, 807, 900
BstV1I GCAGC 6 cut(s) 198, 231, 264, 297, 1091, 1223
BstX2I RGATCY 1 cut(s) 844
BstXI CCANNNNNNTGG 1 cut(s) 707
BstYI RGATCY 1 cut(s) 844
Bsu36I CCTNAGG 3 cut(s) 129, 546, 591
BsuRI GGCC 5 cut(s) 104, 449, 720, 1317, 1382
BtrI CACGTC 1 cut(s) 1274
Cac8I GCNNGC 3 cut(s) 106, 714, 718
CfoI GCGC 1 cut(s) 699
Cfr13I GGNCC 2 cut(s) 17, 1380
CviAII CATG 7 cut(s) 757, 834, 1031, 1178, 1223, 1492, 1531
DpnI GATC 7 cut(s) 90, 198, 231, 264, 297, 360, 846
DpnII GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
EaeI YGGCCR 2 cut(s) 718, 1315
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 309, 450
Eco32I GATATC 2 cut(s) 937, 1360
Eco47I GGWCC 1 cut(s) 17
Eco81I CCTNAGG 3 cut(s) 129, 546, 591
Eco88I CYCGRG 1 cut(s) 275
EcoNI CCTNNNNNAGG 1 cut(s) 887
EcoO109I RGGNCCY 1 cut(s) 1380
EcoRI GAATTC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 724, 1382
EcoRV GATATC 2 cut(s) 937, 1360
EcoT14I CCWWGG 2 cut(s) 309, 450
EcoT22I ATGCAT 1 cut(s) 860
ErhI CCWWGG 2 cut(s) 309, 450
FaeI CATG 7 cut(s) 760, 837, 1034, 1181, 1226, 1495, 1534
FaqI GGGAC 2 cut(s) 150, 257
FatI CATG 7 cut(s) 756, 833, 1030, 1177, 1222, 1491, 1530
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 8 cut(s) 187, 220, 253, 286, 721, 1080, 1212, 1318
Fsp4HI GCNGC 8 cut(s) 187, 220, 253, 286, 721, 1080, 1212, 1318
FspBI CTAG 1 cut(s) 1482
GlaI GCGC 1 cut(s) 698
GluI GCNGC 8 cut(s) 187, 220, 253, 286, 721, 1080, 1212, 1318
HaeIII GGCC 5 cut(s) 104, 449, 720, 1317, 1382
HapII CCGG 1 cut(s) 20
HhaI GCGC 1 cut(s) 699
Hin1II CATG 7 cut(s) 760, 837, 1034, 1181, 1226, 1495, 1534
Hin6I GCGC 1 cut(s) 697
HinP1I GCGC 1 cut(s) 697
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 4 cut(s) 674, 866, 872, 963
HpaII CCGG 1 cut(s) 20
HphI GGTGA 3 cut(s) 407, 698, 1000
Hpy166II GTNNAC 2 cut(s) 6, 418
Hpy188I TCNGA 9 cut(s) 211, 244, 513, 865, 871, 877, 954, 962, 1174
Hpy188III TCNNGA 8 cut(s) 128, 145, 339, 433, 463, 590, 1370, 1511
Hpy8I GTNNAC 2 cut(s) 6, 418
HpyAV CCTTC 5 cut(s) 37, 49, 163, 810, 883
HpyCH4III ACNGT 2 cut(s) 1457, 1504
HpyCH4IV ACGT 1 cut(s) 1273
HpyF10VI GCNNNNNNNGC 6 cut(s) 380, 541, 605, 1114, 1217, 1388
HpySE526I ACGT 1 cut(s) 1273
Hsp92II CATG 7 cut(s) 760, 837, 1034, 1181, 1226, 1495, 1534
HspAI GCGC 1 cut(s) 697
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
LmnI GCTCC 4 cut(s) 796, 1011, 1162, 1291
Lsp1109I GCAGC 6 cut(s) 198, 231, 264, 297, 1091, 1223
LweI GCATC 6 cut(s) 370, 586, 867, 1267, 1329, 1378
MaeI CTAG 1 cut(s) 1482
MaeII ACGT 1 cut(s) 1273
MaeIII GTNAC 1 cut(s) 1340
MalI GATC 7 cut(s) 90, 198, 231, 264, 297, 360, 846
MboI GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
MfeI CAATTG 1 cut(s) 1443
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 1 cut(s) 1159
MlyI GAGTC 2 cut(s) 881, 957
Mph1103I ATGCAT 1 cut(s) 860
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 6 cut(s) 560, 968, 1074, 1283, 1334, 1466
MslI CAYNNNNRTG 1 cut(s) 1272
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 726, 1384
MunI CAATTG 1 cut(s) 1443
Mva1269I GAATGC 2 cut(s) 500, 552
MvaI CCWGG 2 cut(s) 726, 1384
MwoI GCNNNNNNNGC 6 cut(s) 380, 541, 605, 1114, 1217, 1388
NdeII GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
NlaIII CATG 7 cut(s) 760, 837, 1034, 1181, 1226, 1495, 1534
NlaIV GGNNCC 5 cut(s) 798, 839, 1293, 1381, 1489
NsiI ATGCAT 1 cut(s) 860
NspI RCATGY 2 cut(s) 760, 1534
PciI ACATGT 1 cut(s) 1530
PcsI WCGNNNNNNNCGW 1 cut(s) 709
PctI GAATGC 2 cut(s) 500, 552
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 674, 866
PflFI GACNNNGTC 1 cut(s) 458
PflMI CCANNNNNTGG 1 cut(s) 847
PkrI GCNGC 8 cut(s) 188, 221, 254, 287, 722, 1081, 1213, 1319
PleI GAGTC 2 cut(s) 880, 957
PpsI GAGTC 2 cut(s) 880, 957
PscI ACATGT 1 cut(s) 1530
Psp6I CCWGG 2 cut(s) 724, 1382
PspGI CCWGG 2 cut(s) 724, 1382
PspN4I GGNNCC 5 cut(s) 798, 839, 1293, 1381, 1489
PspPI GGNCC 2 cut(s) 17, 1380
PsuI RGATCY 1 cut(s) 844
PsyI GACNNNGTC 1 cut(s) 458
RseI CAYNNNNRTG 1 cut(s) 1272
SaqAI TTAA 6 cut(s) 560, 968, 1074, 1283, 1334, 1466
SatI GCNGC 8 cut(s) 187, 220, 253, 286, 721, 1080, 1212, 1318
Sau3AI GATC 7 cut(s) 88, 196, 229, 262, 295, 358, 844
Sau96I GGNCC 2 cut(s) 17, 1380
SchI GAGTC 2 cut(s) 881, 957
ScrFI CCNGG 2 cut(s) 726, 1384
SduI GDGCHC 1 cut(s) 1159
SfaNI GCATC 6 cut(s) 370, 586, 867, 1267, 1329, 1378
SfcI CTRYAG 3 cut(s) 776, 807, 900
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 1272
SmlI CTYRAG 2 cut(s) 1103, 1215
SmoI CTYRAG 2 cut(s) 1103, 1215
SsiI CCGC 5 cut(s) 50, 721, 1108, 1318, 1427
SspI AATATT 1 cut(s) 508
SspMI CTAG 1 cut(s) 1482
StyD4I CCNGG 2 cut(s) 724, 1382
StyI CCWWGG 2 cut(s) 309, 450
TaaI ACNGT 2 cut(s) 1457, 1504
TaiI ACGT 1 cut(s) 1276
TaqI TCGA 2 cut(s) 66, 361
TauI GCSGC 2 cut(s) 723, 1320
TfiI GAWTC 2 cut(s) 674, 866
Tru1I TTAA 6 cut(s) 560, 968, 1074, 1283, 1334, 1466
Tru9I TTAA 6 cut(s) 560, 968, 1074, 1283, 1334, 1466
TseI GCWGC 6 cut(s) 186, 219, 252, 285, 1079, 1211
TspDTI ATGAA 6 cut(s) 792, 822, 828, 1047, 1194, 1536
TspGWI ACGGA 2 cut(s) 167, 1304
Tth111I GACNNNGTC 1 cut(s) 458
Van91I CCANNNNNTGG 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 17
XagI CCTNNNNNAGG 1 cut(s) 887
XapI RAATTY 5 cut(s) 25, 112, 623, 653, 829
XceI RCATGY 2 cut(s) 760, 1534
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 1 cut(s) 1482
Zsp2I ATGCAT 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.