AT2G19150

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001788
Physical Location & Seq
Forward (+)
8305100 .. 8307580
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G19150.1

Sequence Viewer

Length: 1020 bp
ATGAAGGGGGTGACGATTCACAATTTTTGTTACTCTTACTTCAAGGTGTGTCTACTTGTGATGTCATTGGCATACGGATCCGCCGAATGGGATGGAAGCTCCAGCCAAATAGCTAAAACTATCATCGTCAATCCAAACGATGCTAGATATTTCAAAACTGTCCAAAGTGCAATCGACTCTATCCCTCTGCAAAACCAAGATTGGATCCGTATCCTCATAAGTAACGGTATTTACAGTGAGAAAGTAACAATACCTAGGGGCAAAGGATACATATACATGCAAGGCGGAGGAATTGAAAAGACGATAATAGCTTACGGTGATCATCAACTCACCAATACTAGCGCCACTTTCACGTCCTATCCTAGCAATATCATCATTACTGGAATCACCTTCAAAAATAAATATAATATTGCATCGAGTAGCTCACCAACCAAACCAGCGGTGGCTGCGATGATGCTTGGAGATAAATATGCAATAATCGATTCAAGTTTTGATGGTTTTCAAGATACGCTTTACGACGATTACGGCAGACACTACTACAAACGATGTGTTATCTCCGGTGGCATAGATTTCATTTTTGGCGGTGCCCAATCCATTTTTGAGGGATGTACGTTGAAACTAAGGGTTGGAATCTACCCACCGAATGAAGTGTATGGAACGATAACGGCACAAGGAAGGGACTCGCCGACGGATAAGGGAGGGTTCGTGTTTAAGGACTGCACTGTTATGGGAAGCGGCAAGGCGTTACTGGGACGAGCGTGGAAATCTTATTCACGTGTTATCTTTTACCGTTCTATGTTTAGCGACAATATTTTGCCAATTGGTTGGGATGCTTGGAAAGCAAAAGGCCAAGAGGGGCATATAACGTTTGTGGAGTTTGGTTGCACTGGAGTTGGAGCAGACACGTCAAAGAGAGTGCCATGGCTCACGAAGGCAAGTGAAAAAGACGTTCTTCAGTTTACGAATCTTACCTTTATTGACGAAGAAGGTTGGCTCAGTAGGCTTCCCATCAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

339

Amino Acids

37.63

Weight (kDa)

8.7

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 41 - 328 4.1e-60 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 52
AciI CCGC 5 cut(s) 81, 285, 440, 582, 735
AclI AACGTT 1 cut(s) 866
AclWI GGATC 4 cut(s) 72, 85, 199, 212
AcsI RAATTY 1 cut(s) 1013
AcuI CTGAAG 1 cut(s) 938
AcvI CACGTG 1 cut(s) 776
AfaI GTAC 1 cut(s) 610
AfiI CCNNNNNNNGG 1 cut(s) 87
AflIII ACRYGT 2 cut(s) 775, 903
AgsI TTSAA 7 cut(s) 43, 154, 296, 394, 486, 503, 616
AjiI CACGTC 2 cut(s) 354, 906
AluBI AGCT 4 cut(s) 99, 113, 311, 423
AluI AGCT 4 cut(s) 99, 113, 311, 423
AlwI GGATC 4 cut(s) 72, 85, 199, 212
AoxI GGCC 1 cut(s) 847
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 1013
AspA2I CCTAGG 1 cut(s) 254
AspLEI GCGC 1 cut(s) 344
AsuHPI GGTGA 5 cut(s) 22, 322, 329, 379, 417
AvrII CCTAGG 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 589
BamHI GGATCC 2 cut(s) 77, 204
BanI GGYRCC 1 cut(s) 584
BbrPI CACGTG 1 cut(s) 776
BbvI GCAGC 1 cut(s) 433
BccI CCATC 3 cut(s) 86, 488, 1016
BceAI ACGGC 2 cut(s) 541, 681
BciVI GTATCC 2 cut(s) 221, 260
BclI TGATCA 1 cut(s) 319
BfaI CTAG 4 cut(s) 144, 255, 339, 363
BfoI RGCGCY 1 cut(s) 345
BfuI GTATCC 2 cut(s) 221, 260
BisI GCNGC 2 cut(s) 447, 736
BlnI CCTAGG 1 cut(s) 254
BlsI GCNGC 2 cut(s) 448, 737
BmgBI CACGTC 2 cut(s) 354, 906
BmiI GGNNCC 3 cut(s) 79, 206, 586
BmrI ACTGGG 1 cut(s) 758
BmsI GCATC 4 cut(s) 130, 422, 444, 820
BmuI ACTGGG 1 cut(s) 758
BpmI CTGGAG 2 cut(s) 85, 909
Bsa29I ATCGAT 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 776
BsaBI GATNNNNATC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 254, 920
BsaWI WCCGGW 1 cut(s) 557
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 87
Bse1I ACTGG 3 cut(s) 385, 753, 892
Bse8I GATNNNNATC 1 cut(s) 209
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 254, 920
BseGI GGATG 3 cut(s) 97, 611, 835
BseJI GATNNNNATC 1 cut(s) 209
BseLI CCNNNNNNNGG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 1009
BseNI ACTGG 3 cut(s) 385, 753, 892
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 1 cut(s) 433
BsgI GTGCAG 1 cut(s) 703
BshFI GGCC 1 cut(s) 849
BshNI GGYRCC 1 cut(s) 584
BshVI ATCGAT 1 cut(s) 480
BsiSI CCGG 1 cut(s) 558
BslFI GGGAC 2 cut(s) 692, 765
BslI CCNNNNNNNGG 1 cut(s) 87
BsmFI GGGAC 2 cut(s) 692, 765
BsnI GGCC 1 cut(s) 849
Bsp1286I GDGCHC 1 cut(s) 589
Bsp143I GATC 3 cut(s) 77, 204, 319
Bsp19I CCATGG 1 cut(s) 920
BspACI CCGC 5 cut(s) 81, 285, 440, 582, 735
BspANI GGCC 1 cut(s) 849
BspCNI CTCAG 1 cut(s) 1008
BspDI ATCGAT 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 79, 206, 586
BspPI GGATC 4 cut(s) 72, 85, 199, 212
BspT107I GGYRCC 1 cut(s) 584
BsrI ACTGG 3 cut(s) 385, 753, 892
BssECI CCNNGG 2 cut(s) 254, 920
BssMI GATC 3 cut(s) 77, 204, 319
BssT1I CCWWGG 2 cut(s) 254, 920
Bst4CI ACNGT 6 cut(s) 160, 227, 236, 317, 724, 791
BstBAI YACGTR 1 cut(s) 776
BstDEI CTNAG 2 cut(s) 620, 995
BstDSI CCRYGG 1 cut(s) 920
BstF5I GGATG 3 cut(s) 97, 611, 835
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 3 cut(s) 80, 207, 322
BstMBI GATC 3 cut(s) 77, 204, 319
BstMWI GCNNNNNNNGC 3 cut(s) 446, 839, 1000
BstNSI RCATGY 1 cut(s) 280
BstSLI GKGCMC 1 cut(s) 589
BstV1I GCAGC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 77, 204
BstXI CCANNNNNNTGG 1 cut(s) 825
BstYI RGATCY 2 cut(s) 77, 204
Bsu15I ATCGAT 1 cut(s) 480
BsuI GTATCC 2 cut(s) 221, 260
BsuRI GGCC 1 cut(s) 849
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 920
BtgZI GCGATG 1 cut(s) 464
BtrI CACGTC 2 cut(s) 354, 906
BtsCI GGATG 3 cut(s) 97, 611, 835
BtsIMutI CAGTG 3 cut(s) 241, 720, 885
CfoI GCGC 1 cut(s) 344
ClaI ATCGAT 1 cut(s) 480
Csp6I GTAC 1 cut(s) 609
CviAII CATG 2 cut(s) 277, 921
CviQI GTAC 1 cut(s) 609
DdeI CTNAG 2 cut(s) 620, 995
DpnI GATC 3 cut(s) 79, 206, 321
DpnII GATC 3 cut(s) 77, 204, 319
EciI GGCGGA 2 cut(s) 70, 300
Eco130I CCWWGG 2 cut(s) 254, 920
Eco57I CTGAAG 1 cut(s) 938
Eco72I CACGTG 1 cut(s) 776
EcoT14I CCWWGG 2 cut(s) 254, 920
ErhI CCWWGG 2 cut(s) 254, 920
FaeI CATG 2 cut(s) 280, 924
FalI AAGNNNNNCTT 4 cut(s) 495, 527, 936, 968
FaqI GGGAC 2 cut(s) 692, 765
FatI CATG 2 cut(s) 276, 920
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 447, 736
FokI GGATG 3 cut(s) 104, 618, 842
Fsp4HI GCNGC 2 cut(s) 447, 736
FspBI CTAG 4 cut(s) 144, 255, 339, 363
GlaI GCGC 1 cut(s) 343
GluI GCNGC 2 cut(s) 447, 736
GsuI CTGGAG 2 cut(s) 85, 909
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 1 cut(s) 849
HapII CCGG 1 cut(s) 558
HhaI GCGC 1 cut(s) 344
Hin1II CATG 2 cut(s) 280, 924
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 7 cut(s) 16, 176, 384, 482, 630, 680, 964
HpaII CCGG 1 cut(s) 558
HphI GGTGA 5 cut(s) 22, 322, 329, 379, 417
Hpy166II GTNNAC 2 cut(s) 53, 960
Hpy188III TCNNGA 2 cut(s) 503, 928
Hpy8I GTNNAC 2 cut(s) 53, 960
Hpy99I CGWCG 2 cut(s) 521, 691
HpyAV CCTTC 4 cut(s) 400, 669, 925, 980
HpyCH4III ACNGT 6 cut(s) 160, 227, 236, 317, 724, 791
HpyCH4IV ACGT 6 cut(s) 353, 611, 775, 866, 905, 948
HpyCH4V TGCA 7 cut(s) 170, 190, 280, 413, 473, 720, 885
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 839, 1000
HpyF3I CTNAG 2 cut(s) 620, 995
HpySE526I ACGT 6 cut(s) 353, 611, 775, 866, 905, 948
Hsp92II CATG 2 cut(s) 280, 924
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 3 cut(s) 77, 204, 319
LmnI GCTCC 2 cut(s) 104, 896
LpnPI CCDG 6 cut(s) 115, 366, 450, 571, 734, 873
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 4 cut(s) 130, 422, 444, 820
MaeI CTAG 4 cut(s) 144, 255, 339, 363
MaeII ACGT 6 cut(s) 353, 611, 775, 866, 905, 948
MaeIII GTNAC 5 cut(s) 10, 29, 221, 244, 744
MalI GATC 3 cut(s) 79, 206, 321
MboI GATC 3 cut(s) 77, 204, 319
MboII GAAGA 2 cut(s) 944, 995
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 2 cut(s) 77, 204
MhlI GDGCHC 1 cut(s) 589
MluCI AATT 4 cut(s) 22, 291, 819, 1013
MlyI GAGTC 2 cut(s) 170, 674
MmeI TCCRAC 2 cut(s) 607, 874
MnlI CCTC 6 cut(s) 195, 224, 281, 595, 692, 847
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 2 cut(s) 275, 725
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 1 cut(s) 558
MunI CAATTG 1 cut(s) 819
MwoI GCNNNNNNNGC 3 cut(s) 446, 839, 1000
NcoI CCATGG 1 cut(s) 920
NdeII GATC 3 cut(s) 77, 204, 319
NlaIII CATG 2 cut(s) 280, 924
NlaIV GGNNCC 3 cut(s) 79, 206, 586
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 1 cut(s) 280
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 5 cut(s) 16, 384, 482, 630, 964
PkrI GCNGC 2 cut(s) 448, 737
PleI GAGTC 2 cut(s) 170, 674
PmaCI CACGTG 1 cut(s) 776
PmlI CACGTG 1 cut(s) 776
PpsI GAGTC 2 cut(s) 170, 674
Ppu21I YACGTR 1 cut(s) 776
Psp1406I AACGTT 1 cut(s) 866
PspCI CACGTG 1 cut(s) 776
PspN4I GGNNCC 3 cut(s) 79, 206, 586
PsuI RGATCY 2 cut(s) 77, 204
RsaI GTAC 1 cut(s) 610
RsaNI GTAC 1 cut(s) 609
RseI CAYNNNNRTG 2 cut(s) 275, 725
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 2 cut(s) 447, 736
Sau3AI GATC 3 cut(s) 77, 204, 319
SchI GAGTC 2 cut(s) 170, 674
SduI GDGCHC 1 cut(s) 589
SfaNI GCATC 4 cut(s) 130, 422, 444, 820
SmiMI CAYNNNNRTG 2 cut(s) 275, 725
Sse9I AATT 4 cut(s) 22, 291, 819, 1013
SsiI CCGC 5 cut(s) 81, 285, 440, 582, 735
SspI AATATT 2 cut(s) 409, 811
SspMI CTAG 4 cut(s) 144, 255, 339, 363
StyI CCWWGG 2 cut(s) 254, 920
TaaI ACNGT 6 cut(s) 160, 227, 236, 317, 724, 791
TaiI ACGT 6 cut(s) 356, 614, 778, 869, 908, 951
TaqI TCGA 3 cut(s) 174, 416, 480
TasI AATT 4 cut(s) 22, 291, 819, 1013
TauI GCSGC 1 cut(s) 738
TfiI GAWTC 5 cut(s) 16, 384, 482, 630, 964
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 3 cut(s) 241, 727, 892
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 17, 562, 660
TspGWI ACGGA 3 cut(s) 90, 197, 704
TspRI CASTG 3 cut(s) 241, 727, 892
XapI RAATTY 1 cut(s) 1013
XceI RCATGY 1 cut(s) 280
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XmaJI CCTAGG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 52
XspI CTAG 4 cut(s) 144, 255, 339, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.