Rh3DG083100

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001788
Physical Location & Seq
Reverse (-)
6436238 .. 6436726
489 bp
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UTR
Exon/CDS
Intron
Rh3DG083100.1

Sequence Viewer

Length: 399 bp
ATGAAGTCTCTTATTCTTATTATTTCAGTGATATTGGCTCTTTTCTCTTTTCTGAATGGATTGGATGCATTCCGTCCACTAGACCAAGTTTCAACTACCATCGTAGTTGATCAGAATGGTAAAGGACAATTTACCACTGTTCAACAAGCCGTCGACTCTATACCACCAAATAACCCGTGGACTCTAATTCACCTCAACTCCGGTGTCTACAATGAAAAAGTTGAGATCAAGAAGCCATATATTATTCTTGAAGGAGACCGCGATCAGCAAACTGTGATTCAATATGGAGATGGCGGAAATGTTGTTACCAGTCCAACATTTAAGTTGAATGCGGATAATTTTGTGGCACGATATATTGTATTCAAGGTTAAGCGCCAAACTCAAGTTTTATTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

132

Amino Acids

14.8

Weight (kDa)

7.96

Isoelectric Point (pI)

10.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 34 - 122 3.2e-13 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 153, 207
AccII CGCG 1 cut(s) 261
AciI CCGC 3 cut(s) 259, 294, 332
AgsI TTSAA 6 cut(s) 93, 143, 251, 281, 328, 364
Alw26I GTCTC 2 cut(s) 12, 249
AspLEI GCGC 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 182
BccI CCATC 2 cut(s) 107, 284
BceAI ACGGC 1 cut(s) 134
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 12, 249
BfaI CTAG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 376
BmsI GCATC 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 176
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bse1I ACTGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 1 cut(s) 70
BseNI ACTGG 1 cut(s) 309
Bsh1236I CGCG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 201
BsmAI GTCTC 2 cut(s) 12, 249
BsmI GAATGC 2 cut(s) 68, 334
Bso31I GGTCTC 1 cut(s) 249
Bsp143I GATC 3 cut(s) 109, 225, 262
BspACI CCGC 3 cut(s) 259, 294, 332
BspFNI CGCG 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 249
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 3 cut(s) 109, 225, 262
Bst4CI ACNGT 2 cut(s) 139, 274
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 70
BstFNI CGCG 1 cut(s) 261
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 3 cut(s) 112, 228, 265
BstMAI GTCTC 2 cut(s) 12, 249
BstMBI GATC 3 cut(s) 109, 225, 262
BstUI CGCG 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 176
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 33, 135
CfoI GCGC 1 cut(s) 375
CviJI RGCY 3 cut(s) 38, 149, 235
CviKI_1 RGCY 3 cut(s) 38, 149, 235
DpnI GATC 3 cut(s) 111, 227, 264
DpnII GATC 3 cut(s) 109, 225, 262
EciI GGCGGA 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 70
FaiI YATR 5 cut(s) 161, 238, 240, 285, 354
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 2 cut(s) 153, 207
FokI GGATG 1 cut(s) 77
FspBI CTAG 1 cut(s) 80
GlaI GCGC 1 cut(s) 374
HaeII RGCGCY 1 cut(s) 376
HapII CCGG 1 cut(s) 201
HhaI GCGC 1 cut(s) 375
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 3 cut(s) 155, 181, 277
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 4 cut(s) 77, 154, 180, 208
Hpy188I TCNGA 2 cut(s) 54, 114
Hpy188III TCNNGA 2 cut(s) 229, 248
Hpy8I GTNNAC 4 cut(s) 77, 154, 180, 208
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 2 cut(s) 139, 274
HpyCH4V TGCA 1 cut(s) 68
HspAI GCGC 1 cut(s) 373
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 3 cut(s) 109, 225, 262
LpnPI CCDG 2 cut(s) 214, 322
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 80
MaeIII GTNAC 1 cut(s) 304
MalI GATC 3 cut(s) 111, 227, 264
MboI GATC 3 cut(s) 109, 225, 262
MluCI AATT 3 cut(s) 128, 186, 337
MlyI GAGTC 2 cut(s) 149, 175
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 1 cut(s) 203
Mph1103I ATGCAT 1 cut(s) 70
MseI TTAA 3 cut(s) 321, 369, 397
MspI CCGG 1 cut(s) 201
Mva1269I GAATGC 2 cut(s) 68, 334
MvnI CGCG 1 cut(s) 261
NdeII GATC 3 cut(s) 109, 225, 262
NsiI ATGCAT 1 cut(s) 70
PctI GAATGC 2 cut(s) 68, 334
PfeI GAWTC 1 cut(s) 277
PleI GAGTC 2 cut(s) 149, 175
PpsI GAGTC 2 cut(s) 149, 175
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 3 cut(s) 321, 369, 397
Sau3AI GATC 3 cut(s) 109, 225, 262
SchI GAGTC 2 cut(s) 149, 175
SetI ASST 2 cut(s) 195, 369
SfaNI GCATC 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 381
SmoI CTYRAG 1 cut(s) 381
Sse9I AATT 3 cut(s) 128, 186, 337
SsiI CCGC 3 cut(s) 259, 294, 332
SspMI CTAG 1 cut(s) 80
TaaI ACNGT 2 cut(s) 139, 274
TaqI TCGA 1 cut(s) 153
TasI AATT 3 cut(s) 128, 186, 337
TfiI GAWTC 1 cut(s) 277
Tru1I TTAA 3 cut(s) 321, 369, 397
Tru9I TTAA 3 cut(s) 321, 369, 397
TscAI CASTG 2 cut(s) 33, 142
TspDTI ATGAA 2 cut(s) 17, 228
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 2 cut(s) 33, 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XmiI GTMKAC 2 cut(s) 153, 207
XspI CTAG 1 cut(s) 80
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.