FvH4_1g18330

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001788
Physical Location & Seq
Reverse (-)
10665733 .. 10668302
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18330.t1

Sequence Viewer

Length: 1041 bp
ATGGAGTCTCTCATAACAACAATACTACTCTCATTGCTGGCTCTCTTCTATTTCTTGTCTAAATTGGGTTCATGCTCTCATGCAATGAAATTTGATCTAACTGATGAGGTATCACGCACCTTTAAAGTGGATATGAAGGGCCACGGAGATTTTAGAACAGTGCAAGAAGCCATTGATTCCATCCCTTCAAATAACTCTTGGTGGACTCGAATCATTCTTAACCATGGTCTTTACTTTGAAAAAGTGGTAATTCCTCAAGAGAAACCATACATTATTCTTGAAGGACACCCTAAGTTTCTTACTGCAATTGAATTTGGGGATTTCGGTAGTGTTGGAAAGAGTCCTACATTTACGTTACTTGCTGATAATTTTGTGGCTCGGAATATATTATTCAAGAATTCATATGACCATCTTATTAAAGAGGATCCCAACCGGAGAACTACTTGGGCAGTTGCTGCTAAAATTGTTGGAGACAAAGCTAGTTTTTATCACTGTGGCTTCATTAGCTTGCAAGATACACTCTGGGATGCCAGAGGTCGTCACTATTTTTATGATTGTGATGTTCGTGGTGCTATAGACTTTATCTGGGGCAATGGCCAGTCAATCTATGAGAAATGTCAGATAATGTCTTTGAGCAAAATAATTGGCACTACTGGTTATATCACAGCTCAAGGTCGTGAAGGTCCAGATGAAACTACTGGGTTTGTATTCAAAGATTGTTACGTATGCGGATCAGGCACTGCTTTTCTTGGAAGAGCATACAGAAACTACTCAAGAGTCTTGTTCTCAGGGACGTATATGGAAAATGTTATTACCCCGGAGGGTTGGTCCCTTTGGACGCCACCCCAAATTAGTTATGCGGATGTGATCACATTCTCGGAAGCGAATTGCCGAGGACCTGGAGCAGATTTGTCCCGTCGTATTTCATGGGAGAAGAAGCTATCGGATGAGGAAGTGATTAGTTTAACAAATACAGCTTCTTTCATAGACCAAGATGGCTGGTTGGGAAAGCAACCCCGCGCGTTAAGTCGACCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

347

Amino Acids

39.08

Weight (kDa)

6.19

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 43 - 330 3.4e-55 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1039
AccI GTMKAC 1 cut(s) 1030
AccII CGCG 2 cut(s) 1020, 1022
AciI CCGC 3 cut(s) 729, 860, 1018
AclWI GGATC 3 cut(s) 419, 432, 739
AcoI YGGCCR 1 cut(s) 595
AcsI RAATTY 3 cut(s) 89, 311, 397
AcyI GRCGYC 1 cut(s) 839
AgsI TTSAA 6 cut(s) 189, 239, 281, 311, 394, 712
AjnI CCWGG 1 cut(s) 898
AluBI AGCT 5 cut(s) 479, 507, 668, 940, 977
AluI AGCT 5 cut(s) 479, 507, 668, 940, 977
Alw26I GTCTC 2 cut(s) 12, 465
AlwI GGATC 3 cut(s) 419, 432, 739
AlwNI CAGNNNCTG 2 cut(s) 455, 740
AoxI GGCC 2 cut(s) 139, 595
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 3 cut(s) 89, 311, 397
AspLEI GCGC 1 cut(s) 1022
AspS9I GGNCC 4 cut(s) 139, 683, 828, 896
AsuC2I CCSGG 1 cut(s) 818
AvaII GGWCC 3 cut(s) 683, 828, 896
BalI TGGCCA 1 cut(s) 597
BamHI GGATCC 1 cut(s) 424
BbvI GCAGC 1 cut(s) 442
BccI CCATC 3 cut(s) 188, 417, 989
BciT130I CCWGG 1 cut(s) 900
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 818
BcoDI GTCTC 2 cut(s) 12, 465
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 573
BisI GCNGC 1 cut(s) 456
BlsI GCNGC 1 cut(s) 457
Bme1390I CCNGG 2 cut(s) 818, 900
Bme18I GGWCC 3 cut(s) 683, 828, 896
BmgT120I GGNCC 4 cut(s) 139, 683, 828, 896
BmiI GGNNCC 2 cut(s) 426, 830
BmrFI CCNGG 2 cut(s) 818, 900
BmrI ACTGGG 1 cut(s) 708
BmsI GCATC 1 cut(s) 517
BmuI ACTGGG 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 921
BpuEI CTTGAG 3 cut(s) 240, 654, 757
BpuMI CCSGG 1 cut(s) 818
BsaAI YACGTR 1 cut(s) 724
BsaHI GRCGYC 1 cut(s) 839
BsaJI CCNNGG 4 cut(s) 142, 223, 816, 892
BsaWI WCCGGW 1 cut(s) 432
BsaXI ACNNNNNCTCC 2 cut(s) 812, 842
Bse1I ACTGG 3 cut(s) 598, 658, 703
Bse3DI GCAATG 3 cut(s) 32, 90, 598
BseBI CCWGG 1 cut(s) 900
BseDI CCNNGG 4 cut(s) 142, 223, 816, 892
BseGI GGATG 4 cut(s) 180, 532, 868, 952
BseMI GCAATG 3 cut(s) 32, 90, 598
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 3 cut(s) 598, 658, 703
BseXI GCAGC 1 cut(s) 442
Bsh1236I CGCG 2 cut(s) 1020, 1022
BshFI GGCC 2 cut(s) 141, 597
BsiSI CCGG 2 cut(s) 433, 818
BslFI GGGAC 3 cut(s) 805, 814, 898
BsmAI GTCTC 2 cut(s) 12, 465
BsmFI GGGAC 3 cut(s) 805, 814, 898
BsnI GGCC 2 cut(s) 141, 597
Bsp143I GATC 4 cut(s) 94, 424, 731, 867
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 3 cut(s) 729, 860, 1018
BspANI GGCC 2 cut(s) 141, 597
BspCNI CTCAG 1 cut(s) 800
BspFNI CGCG 2 cut(s) 1020, 1022
BspLI GGNNCC 2 cut(s) 426, 830
BspPI GGATC 3 cut(s) 419, 432, 739
BspQI GCTCTTC 1 cut(s) 748
BsrDI GCAATG 3 cut(s) 32, 90, 598
BsrI ACTGG 3 cut(s) 598, 658, 703
BssECI CCNNGG 4 cut(s) 142, 223, 816, 892
BssMI GATC 4 cut(s) 94, 424, 731, 867
BssNI GRCGYC 1 cut(s) 839
BssT1I CCWWGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 900
Bst4CI ACNGT 2 cut(s) 160, 494
Bst6I CTCTTC 2 cut(s) 50, 748
BstACI GRCGYC 1 cut(s) 839
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 724
BstC8I GCNNGC 2 cut(s) 39, 509
BstDEI CTNAG 2 cut(s) 291, 787
BstDSI CCRYGG 2 cut(s) 142, 223
BstF5I GGATG 4 cut(s) 180, 532, 868, 952
BstFNI CGCG 2 cut(s) 1020, 1022
BstHHI GCGC 1 cut(s) 1022
BstKTI GATC 4 cut(s) 97, 427, 734, 870
BstMAI GTCTC 2 cut(s) 12, 465
BstMBI GATC 4 cut(s) 94, 424, 731, 867
BstMWI GCNNNNNNNGC 3 cut(s) 455, 504, 735
BstNI CCWGG 1 cut(s) 900
BstSCI CCNGG 2 cut(s) 816, 898
BstSFI CTRYAG 1 cut(s) 573
BstSNI TACGTA 1 cut(s) 724
BstUI CGCG 2 cut(s) 1020, 1022
BstV1I GCAGC 1 cut(s) 442
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
BsuRI GGCC 2 cut(s) 141, 597
BtgI CCRYGG 2 cut(s) 142, 223
BtsCI GGATG 4 cut(s) 180, 532, 868, 952
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 3 cut(s) 165, 490, 738
Cac8I GCNNGC 2 cut(s) 39, 509
CaiI CAGNNNCTG 2 cut(s) 455, 740
CfoI GCGC 1 cut(s) 1022
Cfr13I GGNCC 4 cut(s) 139, 683, 828, 896
CseI GACGC 1 cut(s) 847
CviAII CATG 4 cut(s) 72, 80, 224, 927
DdeI CTNAG 2 cut(s) 291, 787
DpnI GATC 4 cut(s) 96, 426, 733, 869
DpnII GATC 4 cut(s) 94, 424, 731, 867
DraI TTTAAA 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 595
Eam1104I CTCTTC 2 cut(s) 50, 748
EarI CTCTTC 2 cut(s) 50, 748
Eco105I TACGTA 1 cut(s) 724
Eco130I CCWWGG 1 cut(s) 223
Eco47I GGWCC 3 cut(s) 683, 828, 896
EcoO109I RGGNCCY 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 898
EcoT14I CCWWGG 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 4 cut(s) 75, 83, 227, 930
FalI AAGNNNNNCTT 1 cut(s) 1018
FaqI GGGAC 3 cut(s) 805, 814, 898
FatI CATG 4 cut(s) 71, 79, 223, 926
FauI CCCGC 1 cut(s) 1025
FauNDI CATATG 1 cut(s) 403
FbaI TGATCA 1 cut(s) 867
FblI GTMKAC 1 cut(s) 1030
Fnu4HI GCNGC 1 cut(s) 456
FokI GGATG 4 cut(s) 167, 539, 875, 959
Fsp4HI GCNGC 1 cut(s) 456
FspBI CTAG 1 cut(s) 480
GlaI GCGC 1 cut(s) 1021
GluI GCNGC 1 cut(s) 456
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 2 cut(s) 141, 597
HapII CCGG 2 cut(s) 433, 818
HgaI GACGC 1 cut(s) 847
HhaI GCGC 1 cut(s) 1022
Hin1I GRCGYC 1 cut(s) 839
Hin1II CATG 4 cut(s) 75, 83, 227, 930
Hin6I GCGC 1 cut(s) 1020
HinP1I GCGC 1 cut(s) 1020
HincII GTYRAC 1 cut(s) 1031
HindII GTYRAC 1 cut(s) 1031
HinfI GANTC 6 cut(s) 5, 176, 205, 210, 340, 777
HpaII CCGG 2 cut(s) 433, 818
Hpy166II GTNNAC 2 cut(s) 204, 1031
Hpy188I TCNGA 4 cut(s) 381, 621, 880, 946
Hpy188III TCNNGA 6 cut(s) 257, 278, 394, 677, 686, 774
Hpy8I GTNNAC 2 cut(s) 204, 1031
Hpy99I CGWCG 1 cut(s) 921
HpyAV CCTTC 4 cut(s) 130, 195, 275, 674
HpyCH4III ACNGT 2 cut(s) 160, 494
HpyCH4IV ACGT 3 cut(s) 353, 723, 794
HpyCH4V TGCA 4 cut(s) 83, 163, 305, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 504, 735
HpyF3I CTNAG 2 cut(s) 291, 787
HpySE526I ACGT 3 cut(s) 353, 723, 794
Hsp92I GRCGYC 1 cut(s) 839
Hsp92II CATG 4 cut(s) 75, 83, 227, 930
HspAI GCGC 1 cut(s) 1020
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 4 cut(s) 94, 424, 731, 867
LguI GCTCTTC 1 cut(s) 748
LmnI GCTCC 1 cut(s) 902
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 1 cut(s) 517
MaeI CTAG 1 cut(s) 480
MaeII ACGT 3 cut(s) 353, 723, 794
MaeIII GTNAC 3 cut(s) 354, 539, 719
MalI GATC 4 cut(s) 96, 426, 733, 869
MboI GATC 4 cut(s) 94, 424, 731, 867
MboII GAAGA 3 cut(s) 37, 765, 946
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 597
MluNI TGGCCA 1 cut(s) 597
MlyI GAGTC 4 cut(s) 14, 199, 349, 786
MmeI TCCRAC 2 cut(s) 313, 448
MnlI CCTC 7 cut(s) 100, 264, 415, 527, 814, 887, 943
Mox20I TGGCCA 1 cut(s) 597
MscI TGGCCA 1 cut(s) 597
MseI TTAA 5 cut(s) 123, 219, 417, 965, 1025
Msp20I TGGCCA 1 cut(s) 597
MspI CCGG 2 cut(s) 433, 818
MspR9I CCNGG 2 cut(s) 818, 900
MunI CAATTG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 900
MvnI CGCG 2 cut(s) 1020, 1022
MwoI GCNNNNNNNGC 3 cut(s) 455, 504, 735
NciI CCSGG 1 cut(s) 818
NcoI CCATGG 1 cut(s) 223
NdeI CATATG 1 cut(s) 403
NdeII GATC 4 cut(s) 94, 424, 731, 867
NlaIII CATG 4 cut(s) 75, 83, 227, 930
NlaIV GGNNCC 2 cut(s) 426, 830
NmeAIII GCCGAG 1 cut(s) 917
NmuCI GTSAC 1 cut(s) 539
PciSI GCTCTTC 1 cut(s) 748
PfeI GAWTC 2 cut(s) 176, 210
PkrI GCNGC 1 cut(s) 457
PleI GAGTC 4 cut(s) 13, 199, 348, 785
PpsI GAGTC 4 cut(s) 13, 199, 348, 785
Ppu21I YACGTR 1 cut(s) 724
PpuMI RGGWCCY 1 cut(s) 896
PsiI TTATAA 1 cut(s) 1039
Psp5II RGGWCCY 1 cut(s) 896
Psp6I CCWGG 1 cut(s) 898
PspGI CCWGG 1 cut(s) 898
PspN4I GGNNCC 2 cut(s) 426, 830
PspPI GGNCC 4 cut(s) 139, 683, 828, 896
PspPPI RGGWCCY 1 cut(s) 896
PstNI CAGNNNCTG 2 cut(s) 455, 740
PsuI RGATCY 1 cut(s) 424
SalI GTCGAC 1 cut(s) 1029
SapI GCTCTTC 1 cut(s) 748
SaqAI TTAA 5 cut(s) 123, 219, 417, 965, 1025
SatI GCNGC 1 cut(s) 456
Sau3AI GATC 4 cut(s) 94, 424, 731, 867
Sau96I GGNCC 4 cut(s) 139, 683, 828, 896
SchI GAGTC 4 cut(s) 14, 199, 349, 786
ScrFI CCNGG 2 cut(s) 818, 900
SfaNI GCATC 1 cut(s) 517
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 3 cut(s) 683, 828, 896
SmlI CTYRAG 3 cut(s) 255, 669, 772
SmoI CTYRAG 3 cut(s) 255, 669, 772
SnaBI TACGTA 1 cut(s) 724
SsiI CCGC 3 cut(s) 729, 860, 1018
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 2 cut(s) 816, 898
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 2 cut(s) 160, 494
TaiI ACGT 3 cut(s) 356, 726, 797
TaqI TCGA 2 cut(s) 208, 1030
TfiI GAWTC 2 cut(s) 176, 210
Tru1I TTAA 5 cut(s) 123, 219, 417, 965, 1025
Tru9I TTAA 5 cut(s) 123, 219, 417, 965, 1025
TscAI CASTG 3 cut(s) 165, 497, 745
TseFI GTSAC 1 cut(s) 539
TseI GCWGC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 8 cut(s) 60, 101, 149, 390, 490, 705, 915, 973
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 3 cut(s) 165, 497, 745
VpaK11BI GGWCC 3 cut(s) 683, 828, 896
XapI RAATTY 3 cut(s) 89, 311, 397
XmiI GTMKAC 1 cut(s) 1030
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.