RLG00000017700

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001788
Physical Location & Seq
Reverse (-)
20188003 .. 20189896
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017700

Sequence Viewer

Length: 792 bp
ATGTCAAGTGAAAAGGTTACAATCCCTCCAGACAAACCGTGCATTATTCTTGAAGGAAACCCTAATCATCTTACGACAATCGAATTTGGAGATGCTGGCAGTGTTGTTGACAGTCCTACATTCAAGGCACATGCAGATAATTTTGTGGCAAAAAATATAGTATTCAAGAATACATACGACCACCTTATCACATTGGAGCCGAATGGGAGGAAAACTACTTGGGCACCTGCTGCTGCAATAGATGGAGACAAAGCAAGTTTCTATAACTGCACTTTCATTAGCTTGCAAGATACACTGTCTGATGCCCAAGGTCGACACTATTTCTATGACTGCAACATTCAAGGTGCTATAGACTTTATTTGGGGAAATGGCCAGTCAATTTACGAGAAATGTCAGATAACTTCGTTGGCCCACAAACTAGGCCAGAATGGTGGTTACATCACTGCACAGGGTCGCGAAAGACCTGAGGAAACTACTGGGTTTGTATTCAAAGATTGCCATGTATGTGGATCAGGACCTATAATGCTAGGAAGAGCATACAGAGCATACGCAAGAGTATTGTTTTCTGGGACATATATGGAAAACATTATTACACCCCAGGGCTGGTCAGAGTGGCAAGTCAAACCAGTGGATTCAATCACATTCTCAGAGGCGAAATGCAGTGGACCTGGAGCGGACATGTCTCATCGGATTCCATGGGAGAAGAAGTTATCGGATGAAGAAGTCGAACAGTTAACAAATACATCTTCTTTTATAAATCAAGATGGATGGTTAGAAAAGCAACCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

264

Amino Acids

29.26

Weight (kDa)

5.35

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 3 - 253 1.8e-46 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 1 cut(s) 223
AccBSI CCGCTC 1 cut(s) 674
AccI GTMKAC 1 cut(s) 313
AccII CGCG 1 cut(s) 456
AciI CCGC 1 cut(s) 674
AclWI GGATC 1 cut(s) 517
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 603
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 6 cut(s) 53, 124, 166, 341, 490, 636
AjnI CCWGG 2 cut(s) 597, 667
AluBI AGCT 1 cut(s) 282
AluI AGCT 1 cut(s) 282
Alw26I GTCTC 2 cut(s) 240, 687
AlwI GGATC 1 cut(s) 517
AoxI GGCC 3 cut(s) 370, 408, 421
ApeKI GCWGC 2 cut(s) 230, 233
ApoI RAATTY 1 cut(s) 83
AspS9I GGNCC 3 cut(s) 409, 515, 665
AvaII GGWCC 2 cut(s) 515, 665
AxyI CCTNAGG 1 cut(s) 465
BaeGI GKGCMC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 372
BanI GGYRCC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 217, 220
BccI CCATC 3 cut(s) 236, 758, 762
BciT130I CCWGG 2 cut(s) 599, 669
BcoDI GTCTC 2 cut(s) 240, 687
BfaI CTAG 2 cut(s) 419, 527
BfmI CTRYAG 1 cut(s) 348
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 2 cut(s) 231, 234
BlsI GCNGC 2 cut(s) 232, 235
Bme1390I CCNGG 2 cut(s) 599, 669
Bme18I GGWCC 2 cut(s) 515, 665
BmgT120I GGNCC 3 cut(s) 409, 515, 665
BmiI GGNNCC 2 cut(s) 198, 225
BmrFI CCNGG 2 cut(s) 599, 669
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 82, 292
BmuI ACTGGG 1 cut(s) 486
BpmI CTGGAG 2 cut(s) 12, 690
BsaJI CCNNGG 4 cut(s) 307, 597, 598, 695
BsaXI ACNNNNNCTCC 4 cut(s) 10, 40, 692, 722
Bsc4I CCNNNNNNNGG 1 cut(s) 603
Bse1I ACTGG 3 cut(s) 373, 481, 626
Bse21I CCTNAGG 1 cut(s) 465
BseBI CCWGG 2 cut(s) 599, 669
BseDI CCNNGG 4 cut(s) 307, 597, 598, 695
BseGI GGATG 2 cut(s) 721, 773
BseLI CCNNNNNNNGG 1 cut(s) 603
BseMII CTCAG 2 cut(s) 456, 660
BseNI ACTGG 3 cut(s) 373, 481, 626
BseSI GKGCMC 1 cut(s) 226
BseXI GCAGC 2 cut(s) 217, 220
BsgI GTGCAG 2 cut(s) 253, 429
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 3 cut(s) 372, 410, 423
BshNI GGYRCC 1 cut(s) 223
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 1 cut(s) 603
BsmAI GTCTC 2 cut(s) 240, 687
BsmFI GGGAC 1 cut(s) 583
BsnI GGCC 3 cut(s) 372, 410, 423
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 1 cut(s) 509
Bsp19I CCATGG 1 cut(s) 695
Bsp68I TCGCGA 1 cut(s) 456
BspACI CCGC 1 cut(s) 674
BspANI GGCC 3 cut(s) 372, 410, 423
BspCNI CTCAG 2 cut(s) 457, 659
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 198, 225
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 1 cut(s) 517
BspQI GCTCTTC 1 cut(s) 526
BspT107I GGYRCC 1 cut(s) 223
BsrBI CCGCTC 1 cut(s) 674
BsrI ACTGG 3 cut(s) 373, 481, 626
BssECI CCNNGG 4 cut(s) 307, 597, 598, 695
BssMI GATC 1 cut(s) 509
BssT1I CCWWGG 2 cut(s) 307, 695
Bst2UI CCWGG 2 cut(s) 599, 669
Bst4CI ACNGT 4 cut(s) 39, 113, 297, 732
Bst6I CTCTTC 1 cut(s) 526
BstAPI GCANNNNNTGC 1 cut(s) 230
BstC8I GCNNGC 2 cut(s) 97, 284
BstDEI CTNAG 2 cut(s) 465, 646
BstDSI CCRYGG 1 cut(s) 695
BstF5I GGATG 2 cut(s) 721, 773
BstFNI CGCG 1 cut(s) 456
BstKTI GATC 1 cut(s) 512
BstMAI GTCTC 2 cut(s) 240, 687
BstMBI GATC 1 cut(s) 509
BstMWI GCNNNNNNNGC 2 cut(s) 230, 542
BstNI CCWGG 2 cut(s) 599, 669
BstNSI RCATGY 2 cut(s) 134, 682
BstSCI CCNGG 2 cut(s) 597, 667
BstSFI CTRYAG 1 cut(s) 348
BstSLI GKGCMC 1 cut(s) 226
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 217, 220
BstXI CCANNNNNNTGG 2 cut(s) 431, 506
Bsu36I CCTNAGG 1 cut(s) 465
BsuRI GGCC 3 cut(s) 372, 410, 423
BtgI CCRYGG 1 cut(s) 695
BtsCI GGATG 2 cut(s) 721, 773
BtsI GCAGTG 3 cut(s) 106, 441, 667
BtsIMutI CAGTG 5 cut(s) 106, 293, 441, 633, 667
BtuMI TCGCGA 1 cut(s) 456
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 97, 284
Cfr13I GGNCC 3 cut(s) 409, 515, 665
CviAII CATG 4 cut(s) 131, 500, 679, 696
CviJI RGCY 6 cut(s) 199, 282, 372, 410, 423, 603
CviKI_1 RGCY 6 cut(s) 199, 282, 372, 410, 423, 603
DdeI CTNAG 2 cut(s) 465, 646
DpnI GATC 1 cut(s) 511
DpnII GATC 1 cut(s) 509
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 526
Eco130I CCWWGG 2 cut(s) 307, 695
Eco47I GGWCC 2 cut(s) 515, 665
Eco81I CCTNAGG 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 597, 667
EcoT14I CCWWGG 2 cut(s) 307, 695
ErhI CCWWGG 2 cut(s) 307, 695
FaeI CATG 4 cut(s) 134, 503, 682, 699
FaqI GGGAC 1 cut(s) 583
FatI CATG 4 cut(s) 130, 499, 678, 695
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 231, 234
FokI GGATG 2 cut(s) 728, 780
Fsp4HI GCNGC 2 cut(s) 231, 234
FspBI CTAG 2 cut(s) 419, 527
GluI GCNGC 2 cut(s) 231, 234
GsuI CTGGAG 2 cut(s) 12, 690
HaeIII GGCC 3 cut(s) 372, 410, 423
Hin1II CATG 4 cut(s) 134, 503, 682, 699
HincII GTYRAC 3 cut(s) 109, 314, 735
HindII GTYRAC 3 cut(s) 109, 314, 735
HinfI GANTC 2 cut(s) 632, 691
HpaI GTTAAC 1 cut(s) 735
Hpy166II GTNNAC 4 cut(s) 109, 314, 665, 735
Hpy188I TCNGA 6 cut(s) 301, 396, 610, 649, 690, 715
Hpy188III TCNNGA 6 cut(s) 29, 50, 166, 455, 513, 761
Hpy8I GTNNAC 4 cut(s) 109, 314, 665, 735
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 4 cut(s) 39, 113, 297, 732
HpyCH4V TGCA 8 cut(s) 42, 134, 236, 270, 286, 333, 446, 660
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 542
HpyF3I CTNAG 2 cut(s) 465, 646
Hsp92II CATG 4 cut(s) 134, 503, 682, 699
KspAI GTTAAC 1 cut(s) 735
Kzo9I GATC 1 cut(s) 509
LguI GCTCTTC 1 cut(s) 526
LmnI GCTCC 2 cut(s) 196, 671
Lsp1109I GCAGC 2 cut(s) 217, 220
LweI GCATC 2 cut(s) 82, 292
MaeI CTAG 2 cut(s) 419, 527
MaeIII GTNAC 2 cut(s) 16, 434
MalI GATC 1 cut(s) 511
MbiI CCGCTC 1 cut(s) 674
MboI GATC 1 cut(s) 509
MboII GAAGA 4 cut(s) 543, 715, 731, 738
MhlI GDGCHC 1 cut(s) 226
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 3 cut(s) 83, 139, 378
MluNI TGGCCA 1 cut(s) 372
MnlI CCTC 4 cut(s) 36, 201, 460, 643
Mox20I TGGCCA 1 cut(s) 372
MscI TGGCCA 1 cut(s) 372
MseI TTAA 2 cut(s) 734, 790
MslI CAYNNNNRTG 1 cut(s) 504
Msp20I TGGCCA 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 599, 669
MvaI CCWGG 2 cut(s) 599, 669
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 230, 542
NcoI CCATGG 1 cut(s) 695
NdeII GATC 1 cut(s) 509
NlaIII CATG 4 cut(s) 134, 503, 682, 699
NlaIV GGNNCC 2 cut(s) 198, 225
NruI TCGCGA 1 cut(s) 456
NspI RCATGY 2 cut(s) 134, 682
PaqCI CACCTGC 1 cut(s) 235
PasI CCCWGGG 1 cut(s) 598
PciI ACATGT 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 632, 691
PkrI GCNGC 2 cut(s) 232, 235
PpuMI RGGWCCY 1 cut(s) 515
PscI ACATGT 1 cut(s) 678
PsiI TTATAA 1 cut(s) 755
Psp5II RGGWCCY 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 597, 667
PspGI CCWGG 2 cut(s) 597, 667
PspN4I GGNNCC 2 cut(s) 198, 225
PspPI GGNCC 3 cut(s) 409, 515, 665
PspPPI RGGWCCY 1 cut(s) 515
RruI TCGCGA 1 cut(s) 456
RseI CAYNNNNRTG 1 cut(s) 504
SalI GTCGAC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 526
SaqAI TTAA 2 cut(s) 734, 790
SatI GCNGC 2 cut(s) 231, 234
Sau3AI GATC 1 cut(s) 509
Sau96I GGNCC 3 cut(s) 409, 515, 665
ScrFI CCNGG 2 cut(s) 599, 669
SduI GDGCHC 1 cut(s) 226
SetI ASST 9 cut(s) 18, 186, 229, 284, 313, 346, 466, 520, 670
SfaNI GCATC 2 cut(s) 82, 292
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 2 cut(s) 515, 665
SmiMI CAYNNNNRTG 1 cut(s) 504
Sse9I AATT 3 cut(s) 83, 139, 378
SsiI CCGC 1 cut(s) 674
SspMI CTAG 2 cut(s) 419, 527
StyD4I CCNGG 2 cut(s) 597, 667
StyI CCWWGG 2 cut(s) 307, 695
TaaI ACNGT 4 cut(s) 39, 113, 297, 732
TaqI TCGA 3 cut(s) 81, 313, 726
TasI AATT 3 cut(s) 83, 139, 378
TfiI GAWTC 2 cut(s) 632, 691
Tru1I TTAA 2 cut(s) 734, 790
Tru9I TTAA 2 cut(s) 734, 790
TscAI CASTG 5 cut(s) 106, 300, 448, 633, 667
TseI GCWGC 2 cut(s) 230, 233
TspDTI ATGAA 2 cut(s) 265, 732
TspRI CASTG 5 cut(s) 106, 300, 448, 633, 667
VpaK11BI GGWCC 2 cut(s) 515, 665
XapI RAATTY 1 cut(s) 83
XceI RCATGY 2 cut(s) 134, 682
XmiI GTMKAC 1 cut(s) 313
XspI CTAG 2 cut(s) 419, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.