Rh2AG207500

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001788
Physical Location & Seq
Reverse (-)
21075029 .. 21084920
9892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG207500.1

Sequence Viewer

Length: 1089 bp
ATGAAGTTTCTCGTAATATTGGTTCTCTTCTTTTTCTTTGCTAAATTTGATGCAATTAGATATGATCTACAAGGTGATGTGTCGAGAACAATGACTGTCAGTAAGAATGGTCGAGGGCAATTTAGCACTGTACAACAAGCCATTGATTCCATACCATCAAATAATGCATGGTGGATCCGGATCCATATTAACCATGGTGTCTACTATGAAAAGGTTACAGTCCCTCAAGACAAACCGTACATTATTCTTGAAGGGAACCCTAATCATCTTACAACAATCGAATTTGGAGATGCTGGCAGTGTTGTTGACAGTCCTACATTCAAGGCACATGCAGATAATTTTGTGGCAAAAAATATAGTATTCAAGAATACTTACGACCATCTTATCACATTGGAGCCGAATGGGAGGAAAACTACTTGGGCACCTGCTGCTGCAATAGATGGAGACAAAGCAAGTTTCTATAACTGCACTTTCATTAGCTTGCAAGATACACTGTCTGATGCCCAAGGTCGACACTATTTCTATGACTGCAACATTCAAGGTGCTATAGACTTCATTTGGGGAAATGGCCAGTCAATTTACGAGAAATGTCAGATAACTTCGTTAGTCCACAAACTAGGCCAGAATGGTGGTTATATCACTGCACAGGGTCGCCAAAGACCTGAGGAAACTACTGGGTTTGTATTCAAAGATTGCCATGTACGTGGATCAGGCCCTATAATGCTAGGAAGAGCATACAGAGCATACGCAAGAGTATTGTTTTCTGGGACATATATGGAAAACATTGTTACACCCCAGGGCTGGTCAGAATGGCAAGTCAAACCAGTGGATTCAATCACATTCTCAGAGACGAAATGCAGTGGACCTGGAGCGGACATGTCTCATCGGATTCCATGGGAGAAGAAGTTATCGGATGAAGAAATCGAACAGTTAACAAATACAGCTTCTTTTATAAATCAAGATGGATGGGATTCAATCACATTCTCAGAGGCGAAATGCAGGATTCAATGGGAGAAGAAGTTATCGAATGATGAAGTCATGAGTTTAGCAATTACAGCTTCTTTTATAAATCAAGATGGACGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

362

Amino Acids

40.87

Weight (kDa)

6.0

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 31 - 319 8e-59 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 953, 1067
AarI CACCTGC 1 cut(s) 433
Acc36I ACCTGC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 421
AccBSI CCGCTC 1 cut(s) 872
AccI GTMKAC 2 cut(s) 201, 511
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 1 cut(s) 872
AclWI GGATC 5 cut(s) 169, 175, 182, 188, 715
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 2 cut(s) 44, 281
AfaI GTAC 3 cut(s) 132, 239, 702
AfiI CCNNNNNNNGG 1 cut(s) 801
AflIII ACRYGT 1 cut(s) 876
AgsI TTSAA 8 cut(s) 251, 322, 364, 539, 688, 834, 975, 1007
AjnI CCWGG 2 cut(s) 795, 865
AluBI AGCT 3 cut(s) 480, 944, 1058
AluI AGCT 3 cut(s) 480, 944, 1058
Alw26I GTCTC 3 cut(s) 438, 842, 885
AlwI GGATC 5 cut(s) 169, 175, 182, 188, 715
Aor13HI TCCGGA 1 cut(s) 177
AoxI GGCC 3 cut(s) 568, 619, 712
ApeKI GCWGC 2 cut(s) 428, 431
ApoI RAATTY 2 cut(s) 44, 281
AspS9I GGNCC 2 cut(s) 713, 863
AsuHPI GGTGA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 863
AxyI CCTNAGG 1 cut(s) 663
BaeGI GKGCMC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 570
BamHI GGATCC 2 cut(s) 174, 180
BanI GGYRCC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 415, 418
BccI CCATC 6 cut(s) 163, 387, 434, 956, 960, 1070
BciT130I CCWGG 2 cut(s) 797, 867
BcoDI GTCTC 3 cut(s) 438, 842, 885
BfaI CTAG 2 cut(s) 617, 725
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 433
BisI GCNGC 2 cut(s) 429, 432
BlsI GCNGC 2 cut(s) 430, 433
Bme1390I CCNGG 2 cut(s) 797, 867
Bme18I GGWCC 1 cut(s) 863
BmgT120I GGNCC 2 cut(s) 713, 863
BmiI GGNNCC 5 cut(s) 176, 182, 257, 396, 423
BmrFI CCNGG 2 cut(s) 797, 867
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 3 cut(s) 40, 280, 490
BmuI ACTGGG 1 cut(s) 684
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 704
BsaBI GATNNNNATC 1 cut(s) 179
BsaJI CCNNGG 5 cut(s) 193, 505, 795, 796, 893
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 4 cut(s) 890, 920, 1004, 1034
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse1I ACTGG 3 cut(s) 571, 679, 824
Bse21I CCTNAGG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 179
BseAI TCCGGA 1 cut(s) 177
BseBI CCWGG 2 cut(s) 797, 867
BseDI CCNNGG 5 cut(s) 193, 505, 795, 796, 893
BseGI GGATG 2 cut(s) 919, 971
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 801
BseMII CTCAG 3 cut(s) 654, 858, 999
BseNI ACTGG 3 cut(s) 571, 679, 824
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 2 cut(s) 415, 418
BsgI GTGCAG 2 cut(s) 451, 627
BshFI GGCC 3 cut(s) 570, 621, 714
BshNI GGYRCC 1 cut(s) 421
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 2 cut(s) 206, 781
BslI CCNNNNNNNGG 1 cut(s) 801
BsmAI GTCTC 3 cut(s) 438, 842, 885
BsmBI CGTCTC 1 cut(s) 842
BsmFI GGGAC 2 cut(s) 206, 781
BsnI GGCC 3 cut(s) 570, 621, 714
Bsp1286I GDGCHC 1 cut(s) 424
Bsp13I TCCGGA 1 cut(s) 177
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 4 cut(s) 64, 174, 180, 707
Bsp19I CCATGG 2 cut(s) 193, 893
BspACI CCGC 1 cut(s) 872
BspANI GGCC 3 cut(s) 570, 621, 714
BspCNI CTCAG 3 cut(s) 655, 857, 998
BspEI TCCGGA 1 cut(s) 177
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 5 cut(s) 176, 182, 257, 396, 423
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 5 cut(s) 169, 175, 182, 188, 715
BspQI GCTCTTC 1 cut(s) 724
BspT107I GGYRCC 1 cut(s) 421
BsrBI CCGCTC 1 cut(s) 872
BsrGI TGTACA 1 cut(s) 130
BsrI ACTGG 3 cut(s) 571, 679, 824
BssECI CCNNGG 5 cut(s) 193, 505, 795, 796, 893
BssMI GATC 4 cut(s) 64, 174, 180, 707
BssT1I CCWWGG 3 cut(s) 193, 505, 893
Bst2UI CCWGG 2 cut(s) 797, 867
Bst4CI ACNGT 7 cut(s) 97, 130, 220, 237, 311, 495, 930
Bst6I CTCTTC 2 cut(s) 32, 724
BstAPI GCANNNNNTGC 1 cut(s) 428
BstAUI TGTACA 1 cut(s) 130
BstBAI YACGTR 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 295, 482
BstDEI CTNAG 3 cut(s) 663, 844, 985
BstDSI CCRYGG 2 cut(s) 193, 893
BstF5I GGATG 2 cut(s) 919, 971
BstKTI GATC 4 cut(s) 67, 177, 183, 710
BstMAI GTCTC 3 cut(s) 438, 842, 885
BstMBI GATC 4 cut(s) 64, 174, 180, 707
BstMWI GCNNNNNNNGC 3 cut(s) 428, 740, 1055
BstNI CCWGG 2 cut(s) 797, 867
BstNSI RCATGY 2 cut(s) 332, 880
BstSCI CCNGG 2 cut(s) 795, 865
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 415, 418
BstX2I RGATCY 2 cut(s) 174, 180
BstXI CCANNNNNNTGG 2 cut(s) 629, 704
BstYI RGATCY 2 cut(s) 174, 180
Bsu36I CCTNAGG 1 cut(s) 663
BsuRI GGCC 3 cut(s) 570, 621, 714
BtgI CCRYGG 2 cut(s) 193, 893
BtsCI GGATG 2 cut(s) 919, 971
BtsI GCAGTG 3 cut(s) 304, 639, 865
BtsIMutI CAGTG 6 cut(s) 126, 304, 491, 639, 831, 865
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 2 cut(s) 295, 482
CciI TCATGA 1 cut(s) 1038
Cfr13I GGNCC 2 cut(s) 713, 863
Csp6I GTAC 3 cut(s) 131, 238, 701
CviAII CATG 7 cut(s) 168, 194, 329, 698, 877, 894, 1039
CviJI RGCY 9 cut(s) 140, 397, 480, 570, 621, 714, 801, 944, 1058
CviKI_1 RGCY 9 cut(s) 140, 397, 480, 570, 621, 714, 801, 944, 1058
CviQI GTAC 3 cut(s) 131, 238, 701
DdeI CTNAG 3 cut(s) 663, 844, 985
DpnI GATC 4 cut(s) 66, 176, 182, 709
DpnII GATC 4 cut(s) 64, 174, 180, 707
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 2 cut(s) 32, 724
EarI CTCTTC 2 cut(s) 32, 724
Eco130I CCWWGG 3 cut(s) 193, 505, 893
Eco47I GGWCC 1 cut(s) 863
Eco81I CCTNAGG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 795, 865
EcoT14I CCWWGG 3 cut(s) 193, 505, 893
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 3 cut(s) 193, 505, 893
Esp3I CGTCTC 1 cut(s) 842
FaeI CATG 7 cut(s) 171, 197, 332, 701, 880, 897, 1042
FaqI GGGAC 2 cut(s) 206, 781
FatI CATG 7 cut(s) 167, 193, 328, 697, 876, 893, 1038
FblI GTMKAC 2 cut(s) 201, 511
Fnu4HI GCNGC 2 cut(s) 429, 432
FokI GGATG 2 cut(s) 926, 978
Fsp4HI GCNGC 2 cut(s) 429, 432
FspBI CTAG 2 cut(s) 617, 725
GluI GCNGC 2 cut(s) 429, 432
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 3 cut(s) 570, 621, 714
HapII CCGG 1 cut(s) 178
Hin1II CATG 7 cut(s) 171, 197, 332, 701, 880, 897, 1042
HincII GTYRAC 3 cut(s) 307, 512, 933
HindII GTYRAC 3 cut(s) 307, 512, 933
HinfI GANTC 5 cut(s) 146, 830, 889, 971, 1003
HpaI GTTAAC 1 cut(s) 933
HpaII CCGG 1 cut(s) 178
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 6 cut(s) 202, 307, 512, 610, 863, 933
Hpy188I TCNGA 7 cut(s) 499, 594, 808, 847, 888, 913, 988
Hpy188III TCNNGA 8 cut(s) 84, 178, 227, 248, 364, 959, 1039, 1073
Hpy8I GTNNAC 6 cut(s) 202, 307, 512, 610, 863, 933
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 7 cut(s) 97, 130, 220, 237, 311, 495, 930
HpyCH4IV ACGT 1 cut(s) 703
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 740, 1055
HpyF3I CTNAG 3 cut(s) 663, 844, 985
HpySE526I ACGT 1 cut(s) 703
Hsp92II CATG 7 cut(s) 171, 197, 332, 701, 880, 897, 1042
Kpn2I TCCGGA 1 cut(s) 177
KspAI GTTAAC 1 cut(s) 933
Kzo9I GATC 4 cut(s) 64, 174, 180, 707
LguI GCTCTTC 1 cut(s) 724
LmnI GCTCC 2 cut(s) 394, 869
Lsp1109I GCAGC 2 cut(s) 415, 418
LweI GCATC 3 cut(s) 40, 280, 490
MaeI CTAG 2 cut(s) 617, 725
MaeII ACGT 1 cut(s) 703
MaeIII GTNAC 2 cut(s) 214, 787
MalI GATC 4 cut(s) 66, 176, 182, 709
MbiI CCGCTC 1 cut(s) 872
MboI GATC 4 cut(s) 64, 174, 180, 707
MboII GAAGA 5 cut(s) 19, 741, 913, 929, 1027
MflI RGATCY 2 cut(s) 174, 180
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 7 cut(s) 44, 54, 119, 281, 337, 576, 1050
MluNI TGGCCA 1 cut(s) 570
MnlI CCTC 5 cut(s) 107, 234, 399, 658, 982
Mox20I TGGCCA 1 cut(s) 570
Mph1103I ATGCAT 1 cut(s) 169
MroI TCCGGA 1 cut(s) 177
MscI TGGCCA 1 cut(s) 570
MseI TTAA 2 cut(s) 189, 932
MslI CAYNNNNRTG 1 cut(s) 702
Msp20I TGGCCA 1 cut(s) 570
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 2 cut(s) 797, 867
MvaI CCWGG 2 cut(s) 797, 867
MwoI GCNNNNNNNGC 3 cut(s) 428, 740, 1055
NcoI CCATGG 2 cut(s) 193, 893
NdeII GATC 4 cut(s) 64, 174, 180, 707
NlaIII CATG 7 cut(s) 171, 197, 332, 701, 880, 897, 1042
NlaIV GGNNCC 5 cut(s) 176, 182, 257, 396, 423
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 332, 880
PagI TCATGA 1 cut(s) 1038
PaqCI CACCTGC 1 cut(s) 433
PasI CCCWGGG 1 cut(s) 796
PciI ACATGT 1 cut(s) 876
PciSI GCTCTTC 1 cut(s) 724
PfeI GAWTC 5 cut(s) 146, 830, 889, 971, 1003
PkrI GCNGC 2 cut(s) 430, 433
Ppu21I YACGTR 1 cut(s) 704
PscI ACATGT 1 cut(s) 876
PsiI TTATAA 2 cut(s) 953, 1067
Psp6I CCWGG 2 cut(s) 795, 865
PspGI CCWGG 2 cut(s) 795, 865
PspN4I GGNNCC 5 cut(s) 176, 182, 257, 396, 423
PspPI GGNCC 2 cut(s) 713, 863
PsrI GAACNNNNNNTAC 1 cut(s) 38
PsuI RGATCY 2 cut(s) 174, 180
RsaI GTAC 3 cut(s) 132, 239, 702
RsaNI GTAC 3 cut(s) 131, 238, 701
RseI CAYNNNNRTG 1 cut(s) 702
SalI GTCGAC 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 724
SaqAI TTAA 2 cut(s) 189, 932
SatI GCNGC 2 cut(s) 429, 432
Sau3AI GATC 4 cut(s) 64, 174, 180, 707
Sau96I GGNCC 2 cut(s) 713, 863
ScrFI CCNGG 2 cut(s) 797, 867
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 3 cut(s) 40, 280, 490
SfcI CTRYAG 1 cut(s) 546
SinI GGWCC 1 cut(s) 863
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
Sse9I AATT 7 cut(s) 44, 54, 119, 281, 337, 576, 1050
SsiI CCGC 1 cut(s) 872
SspI AATATT 1 cut(s) 18
SspMI CTAG 2 cut(s) 617, 725
StyD4I CCNGG 2 cut(s) 795, 865
StyI CCWWGG 3 cut(s) 193, 505, 893
TaaI ACNGT 7 cut(s) 97, 130, 220, 237, 311, 495, 930
TaiI ACGT 1 cut(s) 706
TaqI TCGA 6 cut(s) 83, 112, 279, 511, 924, 1025
TasI AATT 7 cut(s) 44, 54, 119, 281, 337, 576, 1050
TatI WGTACW 1 cut(s) 130
TfiI GAWTC 5 cut(s) 146, 830, 889, 971, 1003
Tru1I TTAA 2 cut(s) 189, 932
Tru9I TTAA 2 cut(s) 189, 932
TscAI CASTG 6 cut(s) 133, 304, 498, 646, 831, 865
TseI GCWGC 2 cut(s) 428, 431
TspDTI ATGAA 6 cut(s) 17, 222, 463, 544, 930, 1047
TspRI CASTG 6 cut(s) 133, 304, 498, 646, 831, 865
VpaK11BI GGWCC 1 cut(s) 863
XapI RAATTY 2 cut(s) 44, 281
XceI RCATGY 2 cut(s) 332, 880
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
XmiI GTMKAC 2 cut(s) 201, 511
XspI CTAG 2 cut(s) 617, 725
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.