MD12G1205100.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001788
Physical Location & Seq
Forward (+)
28570488 .. 28573604
3117 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1205100.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGCAGTTTCTAATTATATCTTCAACATTGGCTCTCTTCTTTTTATTGGGTGCAGTGGATTTAAGGTATGTCCCAGGGATCTATAATCTTCAAGACAATGTTATAAGTACCATCATAGTCGATCCGAATGGCCTAGGAAATTTTACCTCGGTGCAACAAGCCATTGATTCCGTAGCACCAAATAACACTGCGTGGACTCGAATTCACATCAACTACGGTATCTACACTGAAAAAGTTCAGATCGCAGTAGACAAACCATACATCTATATTGAAGGAGACTCTGATCGCCAACCTGTGATCCAATATGCGGACGGTGGAAGTATTCTCACTAGTCCTACATTTAAAGCACGTGCAGATAATTTTGTGGCACGAAATATAATCTTCAAGAATACATACGATAATATTGGTACTGACCTATTGGATGCCAATGGAAAAAGGAAGACCACTACATGGGCAGTTGCCCTAGATGTATCTGGAGATAAAGCAAGTTTTTATCACTGTAGCTTTTCTAGCTTGCAAGACACATTGAGTGATGCCAAAGGTCGTCACTATTTCTACGATTGCACCATTGAAGGACTTATAGACTTCATTTGGGGCCATGGCCAATCAATTTATGAGAAATGCACACTAATTTCACGAACGGACGTAATAGGGGGAACTGCCTATATTACAGCCAATGGTCGAGGTGGAGCAGACGACCCCTCTGGGTTTGTATTCAAAGATTGCCATATTACTGGAAGTGGCCCTGCTAATCTGGGAAGGGCATATGGGACTCATTCAAGAGTATTGTTTTACCGGACAGATATGGACAATATTATCACCCCACAGGGTTGGGGTAATCCATGGAATGCAGGCCATGAGGGTACAATTGAGTTTGCTGAGGTGGAATGCAAAGGAGATGGAGCAAACAGGGAACACCGTATCAACTGGGAGAAGAACCAAACGGCTCAGGAAGTGAGGATTTTAACAGATACAGCTTCTTTCATAAACCAAGATGGATGGCTAGAAAAGCACCCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

341

Amino Acids

37.69

Weight (kDa)

5.23

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 38 - 317 1.3e-51 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 450
AccI GTMKAC 1 cut(s) 249
AciI CCGC 1 cut(s) 308
AclWI GGATC 3 cut(s) 86, 116, 292
AcoI YGGCCR 1 cut(s) 601
AcsI RAATTY 2 cut(s) 139, 201
AcvI CACGTG 1 cut(s) 350
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 3 cut(s) 109, 409, 865
AfiI CCNNNNNNNGG 2 cut(s) 307, 450
AgsI TTSAA 7 cut(s) 24, 92, 272, 385, 572, 718, 780
AhlI ACTAGT 1 cut(s) 329
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 3 cut(s) 504, 513, 977
AluI AGCT 3 cut(s) 504, 513, 977
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 3 cut(s) 86, 116, 292
AoxI GGCC 5 cut(s) 130, 595, 601, 742, 853
ApoI RAATTY 2 cut(s) 139, 201
Asp700I GAANNNNTTC 1 cut(s) 234
AspA2I CCTAGG 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 595, 743
AsuHPI GGTGA 1 cut(s) 811
AvrII CCTAGG 1 cut(s) 133
BaeI ACNNNNGTAYC 2 cut(s) 202, 235
BalI TGGCCA 1 cut(s) 603
BbrPI CACGTG 1 cut(s) 350
BbsI GAAGAC 1 cut(s) 446
BbvCI CCTCAGC 1 cut(s) 879
BccI CCATC 4 cut(s) 119, 893, 989, 993
BceAI ACGGC 1 cut(s) 960
BciT130I CCWGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 5 cut(s) 134, 330, 464, 510, 1004
BfmI CTRYAG 1 cut(s) 499
BlnI CCTAGG 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 75
BmgT120I GGNCC 2 cut(s) 595, 743
BmiI GGNNCC 1 cut(s) 596
BmrFI CCNGG 1 cut(s) 75
BmrI ACTGGG 1 cut(s) 937
BmsI GCATC 2 cut(s) 412, 523
BmuI ACTGGG 1 cut(s) 937
BpiI GAAGAC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 2 cut(s) 879, 948
BsaAI YACGTR 1 cut(s) 350
BsaJI CCNNGG 6 cut(s) 73, 74, 133, 147, 598, 842
BsaWI WCCGGW 1 cut(s) 795
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 450
Bse1I ACTGG 2 cut(s) 739, 932
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 6 cut(s) 73, 74, 133, 147, 598, 842
BseGI GGATG 2 cut(s) 427, 1004
BseLI CCNNNNNNNGG 2 cut(s) 307, 450
BseMII CTCAG 2 cut(s) 870, 962
BseNI ACTGG 2 cut(s) 739, 932
BsgI GTGCAG 2 cut(s) 72, 372
BshFI GGCC 5 cut(s) 132, 597, 603, 744, 855
BsiSI CCGG 1 cut(s) 796
BslFI GGGAC 2 cut(s) 56, 784
BslI CCNNNNNNNGG 2 cut(s) 307, 450
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 2 cut(s) 56, 784
BsmI GAATGC 2 cut(s) 853, 893
BsnI GGCC 5 cut(s) 132, 597, 603, 744, 855
Bsp143I GATC 5 cut(s) 78, 121, 240, 283, 297
Bsp19I CCATGG 2 cut(s) 598, 842
BspACI CCGC 1 cut(s) 308
BspANI GGCC 5 cut(s) 132, 597, 603, 744, 855
BspCNI CTCAG 2 cut(s) 871, 961
BspLI GGNNCC 1 cut(s) 596
BspPI GGATC 3 cut(s) 86, 116, 292
BsrI ACTGG 2 cut(s) 739, 932
BssECI CCNNGG 6 cut(s) 73, 74, 133, 147, 598, 842
BssMI GATC 5 cut(s) 78, 121, 240, 283, 297
BssT1I CCWWGG 3 cut(s) 133, 598, 842
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 4 cut(s) 218, 314, 500, 920
Bst6I CTCTTC 1 cut(s) 41
BstBAI YACGTR 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 515, 853
BstDEI CTNAG 2 cut(s) 879, 948
BstDSI CCRYGG 2 cut(s) 598, 842
BstF5I GGATG 2 cut(s) 427, 1004
BstKTI GATC 5 cut(s) 81, 124, 243, 286, 300
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 5 cut(s) 78, 121, 240, 283, 297
BstMWI GCNNNNNNNGC 2 cut(s) 510, 1009
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 78
BstXI CCANNNNNNTGG 2 cut(s) 734, 831
BstYI RGATCY 1 cut(s) 78
BsuRI GGCC 5 cut(s) 132, 597, 603, 744, 855
BtgI CCRYGG 2 cut(s) 598, 842
BtsCI GGATG 2 cut(s) 427, 1004
BtsI GCAGTG 2 cut(s) 60, 186
BtsIMutI CAGTG 4 cut(s) 60, 186, 225, 496
Cac8I GCNNGC 2 cut(s) 515, 853
Cfr13I GGNCC 2 cut(s) 595, 743
Csp6I GTAC 3 cut(s) 108, 408, 864
CviAII CATG 4 cut(s) 450, 599, 843, 857
CviQI GTAC 3 cut(s) 108, 408, 864
DdeI CTNAG 2 cut(s) 879, 948
DpnI GATC 5 cut(s) 80, 123, 242, 285, 299
DpnII GATC 5 cut(s) 78, 121, 240, 283, 297
DraI TTTAAA 1 cut(s) 343
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 3 cut(s) 133, 598, 842
Eco72I CACGTG 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 201
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 3 cut(s) 133, 598, 842
ErhI CCWWGG 3 cut(s) 133, 598, 842
FaeI CATG 4 cut(s) 453, 602, 846, 860
FaqI GGGAC 2 cut(s) 56, 784
FatI CATG 4 cut(s) 449, 598, 842, 856
FauNDI CATATG 1 cut(s) 766
FblI GTMKAC 1 cut(s) 249
FokI GGATG 2 cut(s) 434, 1011
FspBI CTAG 5 cut(s) 134, 330, 464, 510, 1004
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 5 cut(s) 132, 597, 603, 744, 855
HapII CCGG 1 cut(s) 796
Hin1II CATG 4 cut(s) 453, 602, 846, 860
HinfI GANTC 4 cut(s) 167, 196, 278, 772
HpaII CCGG 1 cut(s) 796
HphI GGTGA 1 cut(s) 811
Hpy166II GTNNAC 2 cut(s) 195, 250
Hpy188I TCNGA 3 cut(s) 126, 240, 283
Hpy188III TCNNGA 6 cut(s) 92, 385, 474, 636, 780, 950
Hpy8I GTNNAC 2 cut(s) 195, 250
HpyAV CCTTC 3 cut(s) 266, 566, 753
HpyCH4III ACNGT 4 cut(s) 218, 314, 500, 920
HpyCH4IV ACGT 2 cut(s) 349, 645
HpyCH4V TGCA 9 cut(s) 4, 53, 154, 353, 517, 564, 624, 851, 891
HpyF10VI GCNNNNNNNGC 2 cut(s) 510, 1009
HpyF3I CTNAG 2 cut(s) 879, 948
HpySE526I ACGT 2 cut(s) 349, 645
Hsp92II CATG 4 cut(s) 453, 602, 846, 860
Kzo9I GATC 5 cut(s) 78, 121, 240, 283, 297
LmnI GCTCC 2 cut(s) 689, 902
LweI GCATC 2 cut(s) 412, 523
MaeI CTAG 5 cut(s) 134, 330, 464, 510, 1004
MaeII ACGT 2 cut(s) 349, 645
MaeIII GTNAC 1 cut(s) 545
MalI GATC 5 cut(s) 80, 123, 242, 285, 299
MboI GATC 5 cut(s) 78, 121, 240, 283, 297
MboII GAAGA 6 cut(s) 12, 28, 80, 373, 451, 946
MfeI CAATTG 1 cut(s) 867
MflI RGATCY 1 cut(s) 78
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 8 cut(s) 12, 139, 201, 358, 609, 630, 867, 1018
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 3 cut(s) 190, 272, 766
MnlI CCTC 6 cut(s) 157, 677, 712, 853, 874, 951
Mox20I TGGCCA 1 cut(s) 603
MroXI GAANNNNTTC 1 cut(s) 234
MscI TGGCCA 1 cut(s) 603
MseI TTAA 4 cut(s) 62, 342, 965, 1021
Msp20I TGGCCA 1 cut(s) 603
MspI CCGG 1 cut(s) 796
MspR9I CCNGG 1 cut(s) 75
MunI CAATTG 1 cut(s) 867
Mva1269I GAATGC 2 cut(s) 853, 893
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 510, 1009
NcoI CCATGG 2 cut(s) 598, 842
NdeI CATATG 1 cut(s) 766
NdeII GATC 5 cut(s) 78, 121, 240, 283, 297
NlaIII CATG 4 cut(s) 453, 602, 846, 860
NlaIV GGNNCC 1 cut(s) 596
NmuCI GTSAC 1 cut(s) 545
PasI CCCWGGG 1 cut(s) 74
PctI GAATGC 2 cut(s) 853, 893
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 450
PleI GAGTC 3 cut(s) 190, 272, 766
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
PpsI GAGTC 3 cut(s) 190, 272, 766
Ppu21I YACGTR 1 cut(s) 350
PsiI TTATAA 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 73
PspCI CACGTG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 596
PspPI GGNCC 2 cut(s) 595, 743
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 3 cut(s) 109, 409, 865
RsaNI GTAC 3 cut(s) 108, 408, 864
SaqAI TTAA 4 cut(s) 62, 342, 965, 1021
Sau3AI GATC 5 cut(s) 78, 121, 240, 283, 297
Sau96I GGNCC 2 cut(s) 595, 743
SchI GAGTC 3 cut(s) 190, 272, 766
ScrFI CCNGG 1 cut(s) 75
SfaNI GCATC 2 cut(s) 412, 523
SfcI CTRYAG 1 cut(s) 499
SpeI ACTAGT 1 cut(s) 329
Sse9I AATT 8 cut(s) 12, 139, 201, 358, 609, 630, 867, 1018
SsiI CCGC 1 cut(s) 308
SspI AATATT 2 cut(s) 403, 814
SspMI CTAG 5 cut(s) 134, 330, 464, 510, 1004
StyD4I CCNGG 1 cut(s) 73
StyI CCWWGG 3 cut(s) 133, 598, 842
TaaI ACNGT 4 cut(s) 218, 314, 500, 920
TaiI ACGT 2 cut(s) 352, 648
TaqI TCGA 3 cut(s) 120, 199, 682
TasI AATT 8 cut(s) 12, 139, 201, 358, 609, 630, 867, 1018
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 4 cut(s) 62, 342, 965, 1021
Tru9I TTAA 4 cut(s) 62, 342, 965, 1021
TscAI CASTG 4 cut(s) 60, 193, 232, 503
TseFI GTSAC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 2 cut(s) 577, 973
TspGWI ACGGA 2 cut(s) 160, 656
TspRI CASTG 4 cut(s) 60, 193, 232, 503
Van91I CCANNNNNTGG 1 cut(s) 450
XapI RAATTY 2 cut(s) 139, 201
XmaJI CCTAGG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 5 cut(s) 134, 330, 464, 510, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.