FvH4_6g07550

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001788
Physical Location & Seq
Reverse (-)
4527316 .. 4528557
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07550.t1

Sequence Viewer

Length: 846 bp
ATGGAGTCCTTTATTATTTCTGTTACATTGGCTCTTTTCTTTTTACTGAATGGATTGGATGCAAGGAAATATGGTCTAGTACTAGACCAAGAATCAGCTGCCATTGTAGTCGATAAGAATGGTAAAGGACAATTTACCACTGTTCAACAAGCCGTCGATTCTATACCATCAAATAATTCGGCATGGACTCAAATTTACATTAACCCCGGTGTTTACAATGAAAAAGTTGAGATCAAGAAACCATATATCATTCTCAAAGGAGACTACCACAACCGACCTGTGATTGAATTTGGAGATAGCGGAAGTGTTGTTGACAGTCCTACATTTAAGTTGAATGCTGATAATTTCGTGGCACGAAATATTGTATTCAAGAATACCTATGATCATCTTCTTCCTTCTAAATTAGAAGCCAACAGGGTCAACACTACTTGGGCACCTGCAGCTGCAATTGCCGGAGACAAAGCAAGTTTTTACGATTGCAACTTCATCAGCTTGCAGGACACATTGACTGATGCCACCGGTCGGCATTACTTCCGCCACTGCAACATTTCCGGCGCCATAGACTTCATTTGGGGAAATGGACAATCAATCTACCAGAATTGCGATATTAATTCATTGGCATCAAAAATAGCTCCAGTGGCGGGTTATATTACAGCACAGGCTCGTGAAAAGCCAGAGGAAACCACTGGTTTTGTATTCAACAATTGTCATGTAACTGGATCTGGTCGTATAAATCTAGGAAGAGCTCATACAAAATACGCAAGAGTATTGTTTGCTGGGACAATAATGAACAATATCATCACCCCACAAGGTTGGGACGCAGCAAGCAGTCAGGGTTCCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

282

Amino Acids

30.86

Weight (kDa)

6.3

Isoelectric Point (pI)

30.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 35 - 274 3.1e-47 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
Acc36I ACCTGC 1 cut(s) 445
AccB1I GGYRCC 2 cut(s) 433, 554
AciI CCGC 3 cut(s) 300, 535, 641
AclWI GGATC 1 cut(s) 727
AcsI RAATTY 2 cut(s) 192, 287
AcyI GRCGYC 1 cut(s) 555
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 522, 641
AgeI ACCGGT 1 cut(s) 518
AgsI TTSAA 5 cut(s) 146, 287, 334, 370, 700
AluBI AGCT 5 cut(s) 98, 443, 492, 632, 746
AluI AGCT 5 cut(s) 98, 443, 492, 632, 746
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 2 cut(s) 255, 450
AlwI GGATC 1 cut(s) 727
ApeKI GCWGC 4 cut(s) 98, 440, 443, 821
ApoI RAATTY 2 cut(s) 192, 287
AseI ATTAAT 1 cut(s) 609
AsiGI ACCGGT 1 cut(s) 518
AspLEI GCGC 1 cut(s) 557
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 2 cut(s) 433, 554
BanII GRGCYC 1 cut(s) 748
BarI GAAGNNNNNNTAC 2 cut(s) 733, 765
BauI CACGAG 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 4 cut(s) 85, 430, 452, 833
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 137
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 255, 450
BfaI CTAG 3 cut(s) 77, 83, 737
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 558
BfuAI ACCTGC 1 cut(s) 445
BisI GCNGC 4 cut(s) 99, 441, 444, 822
BlsI GCNGC 4 cut(s) 100, 442, 445, 823
BmcAI AGTACT 1 cut(s) 81
Bme1390I CCNGG 1 cut(s) 207
BmiI GGNNCC 3 cut(s) 435, 556, 838
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 3 cut(s) 49, 502, 629
BpmI CTGGAG 1 cut(s) 618
BpuMI CCSGG 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 555
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 1 cut(s) 518
Bsc4I CCNNNNNNNGG 2 cut(s) 522, 641
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 3 cut(s) 635, 691, 721
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 64
BseLI CCNNNNNNNGG 2 cut(s) 522, 641
BseNI ACTGG 3 cut(s) 635, 691, 721
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 4 cut(s) 85, 430, 452, 833
BseYI CCCAGC 1 cut(s) 776
Bsh1285I CGRYCG 1 cut(s) 523
BshNI GGYRCC 2 cut(s) 433, 554
BshTI ACCGGT 1 cut(s) 518
BsiEI CGRYCG 1 cut(s) 523
BsiHKAI GWGCWC 1 cut(s) 748
BsiSI CCGG 4 cut(s) 207, 453, 519, 552
BslFI GGGAC 2 cut(s) 793, 830
BslI CCNNNNNNNGG 2 cut(s) 522, 641
BsmAI GTCTC 2 cut(s) 255, 450
BsmFI GGGAC 2 cut(s) 793, 830
BsmI GAATGC 1 cut(s) 340
Bsp1286I GDGCHC 2 cut(s) 436, 748
Bsp143I GATC 3 cut(s) 231, 382, 719
BspACI CCGC 3 cut(s) 300, 535, 641
BspLI GGNNCC 3 cut(s) 435, 556, 838
BspMAI CTGCAG 1 cut(s) 442
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 1 cut(s) 727
BspQI GCTCTTC 1 cut(s) 736
BspT107I GGYRCC 2 cut(s) 433, 554
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 3 cut(s) 635, 691, 721
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 3 cut(s) 231, 382, 719
BssNI GRCGYC 1 cut(s) 555
BssSI CACGAG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 663
Bst4CI ACNGT 2 cut(s) 142, 317
Bst6I CTCTTC 1 cut(s) 736
BstACI GRCGYC 1 cut(s) 555
BstC8I GCNNGC 2 cut(s) 494, 826
BstF5I GGATG 1 cut(s) 64
BstH2I RGCGCY 1 cut(s) 558
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 3 cut(s) 234, 385, 722
BstMAI GTCTC 2 cut(s) 255, 450
BstMBI GATC 3 cut(s) 231, 382, 719
BstMCI CGRYCG 1 cut(s) 523
BstMWI GCNNNNNNNGC 3 cut(s) 440, 449, 638
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 4 cut(s) 85, 430, 452, 833
BstX2I RGATCY 1 cut(s) 719
BstXI CCANNNNNNTGG 1 cut(s) 813
BstYI RGATCY 1 cut(s) 719
BtsCI GGATG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 538
BtsIMutI CAGTG 4 cut(s) 138, 538, 642, 684
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 2 cut(s) 494, 826
CfoI GCGC 1 cut(s) 557
Cfr10I RCCGGY 1 cut(s) 518
CseI GACGC 1 cut(s) 827
Csp6I GTAC 1 cut(s) 80
CspAI ACCGGT 1 cut(s) 518
CspCI CAANNNNNGTGG 2 cut(s) 673, 708
CviAII CATG 2 cut(s) 183, 710
CviQI GTAC 1 cut(s) 80
DinI GGCGCC 1 cut(s) 556
DpnI GATC 3 cut(s) 233, 384, 721
DpnII GATC 3 cut(s) 231, 382, 719
Eam1104I CTCTTC 1 cut(s) 736
EarI CTCTTC 1 cut(s) 736
EciI GGCGGA 1 cut(s) 524
Ecl136II GAGCTC 1 cut(s) 746
Eco24I GRGCYC 1 cut(s) 748
Eco53kI GAGCTC 1 cut(s) 746
EcoICRI GAGCTC 1 cut(s) 746
EcoT38I GRGCYC 1 cut(s) 748
EgeI GGCGCC 1 cut(s) 556
EheI GGCGCC 1 cut(s) 556
FaeI CATG 2 cut(s) 186, 713
FaqI GGGAC 2 cut(s) 793, 830
FatI CATG 2 cut(s) 182, 709
FauI CCCGC 1 cut(s) 634
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 4 cut(s) 99, 441, 444, 822
FokI GGATG 1 cut(s) 71
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 4 cut(s) 99, 441, 444, 822
FspBI CTAG 3 cut(s) 77, 83, 737
GlaI GCGC 1 cut(s) 556
GluI GCNGC 4 cut(s) 99, 441, 444, 822
GsaI CCCAGC 1 cut(s) 780
GsuI CTGGAG 1 cut(s) 618
HaeII RGCGCY 1 cut(s) 558
HapII CCGG 4 cut(s) 207, 453, 519, 552
HgaI GACGC 1 cut(s) 827
HhaI GCGC 1 cut(s) 557
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 2 cut(s) 186, 713
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HincII GTYRAC 2 cut(s) 313, 421
HindII GTYRAC 2 cut(s) 313, 421
HinfI GANTC 4 cut(s) 5, 92, 158, 187
HpaII CCGG 4 cut(s) 207, 453, 519, 552
HphI GGTGA 1 cut(s) 793
Hpy166II GTNNAC 3 cut(s) 214, 313, 421
Hpy188I TCNGA 1 cut(s) 841
Hpy188III TCNNGA 3 cut(s) 235, 370, 665
Hpy8I GTNNAC 3 cut(s) 214, 313, 421
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 1 cut(s) 405
HpyCH4III ACNGT 2 cut(s) 142, 317
HpyCH4V TGCA 6 cut(s) 62, 440, 446, 480, 496, 543
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 449, 638
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 2 cut(s) 186, 713
HspAI GCGC 1 cut(s) 555
KasI GGCGCC 1 cut(s) 554
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 231, 382, 719
LguI GCTCTTC 1 cut(s) 736
LmnI GCTCC 1 cut(s) 637
Lsp1109I GCAGC 4 cut(s) 85, 430, 452, 833
LweI GCATC 3 cut(s) 49, 502, 629
MaeI CTAG 3 cut(s) 77, 83, 737
MaeIII GTNAC 2 cut(s) 22, 712
MalI GATC 3 cut(s) 233, 384, 721
MboI GATC 3 cut(s) 231, 382, 719
MboII GAAGA 3 cut(s) 380, 383, 753
MfeI CAATTG 2 cut(s) 447, 703
MflI RGATCY 1 cut(s) 719
MhlI GDGCHC 2 cut(s) 436, 748
Mly113I GGCGCC 1 cut(s) 555
MlyI GAGTC 2 cut(s) 14, 181
MnlI CCTC 1 cut(s) 670
MseI TTAA 3 cut(s) 201, 327, 609
MspA1I CMGCKG 2 cut(s) 98, 443
MspI CCGG 4 cut(s) 207, 453, 519, 552
MspR9I CCNGG 1 cut(s) 207
MunI CAATTG 2 cut(s) 447, 703
Mva1269I GAATGC 1 cut(s) 340
MwoI GCNNNNNNNGC 3 cut(s) 440, 449, 638
NarI GGCGCC 1 cut(s) 555
NciI CCSGG 1 cut(s) 207
NdeII GATC 3 cut(s) 231, 382, 719
NlaIII CATG 2 cut(s) 186, 713
NlaIV GGNNCC 3 cut(s) 435, 556, 838
PaqCI CACCTGC 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 736
PctI GAATGC 1 cut(s) 340
PfeI GAWTC 2 cut(s) 92, 158
PinAI ACCGGT 1 cut(s) 518
PkrI GCNGC 4 cut(s) 100, 442, 445, 823
PleI GAGTC 2 cut(s) 13, 181
PluTI GGCGCC 1 cut(s) 558
PpsI GAGTC 2 cut(s) 13, 181
PshBI ATTAAT 1 cut(s) 609
Psp124BI GAGCTC 1 cut(s) 748
PspFI CCCAGC 1 cut(s) 776
PspN4I GGNNCC 3 cut(s) 435, 556, 838
PstI CTGCAG 1 cut(s) 442
PsuI RGATCY 1 cut(s) 719
PvuII CAGCTG 2 cut(s) 98, 443
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SacI GAGCTC 1 cut(s) 748
SapI GCTCTTC 1 cut(s) 736
SaqAI TTAA 3 cut(s) 201, 327, 609
SatI GCNGC 4 cut(s) 99, 441, 444, 822
Sau3AI GATC 3 cut(s) 231, 382, 719
ScaI AGTACT 1 cut(s) 81
SchI GAGTC 2 cut(s) 14, 181
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 2 cut(s) 436, 748
SetI ASST 9 cut(s) 100, 280, 380, 439, 445, 494, 634, 748, 814
SfaNI GCATC 3 cut(s) 49, 502, 629
SfcI CTRYAG 1 cut(s) 438
SfoI GGCGCC 1 cut(s) 556
SsiI CCGC 3 cut(s) 300, 535, 641
SspDI GGCGCC 1 cut(s) 554
SspI AATATT 1 cut(s) 361
SspMI CTAG 3 cut(s) 77, 83, 737
SstI GAGCTC 1 cut(s) 748
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 142, 317
TaqI TCGA 2 cut(s) 111, 156
TatI WGTACW 1 cut(s) 79
TfiI GAWTC 2 cut(s) 92, 158
Tru1I TTAA 3 cut(s) 201, 327, 609
Tru9I TTAA 3 cut(s) 201, 327, 609
TscAI CASTG 4 cut(s) 145, 545, 642, 691
TseI GCWGC 4 cut(s) 98, 440, 443, 821
TspDTI ATGAA 5 cut(s) 234, 475, 556, 603, 803
TspRI CASTG 4 cut(s) 145, 545, 642, 691
VspI ATTAAT 1 cut(s) 609
XapI RAATTY 2 cut(s) 192, 287
XspI CTAG 3 cut(s) 77, 83, 737
ZrmI AGTACT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.