Rh2DG213900

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001788
Physical Location & Seq
Reverse (-)
20405379 .. 20420263
14885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG213900.1

Sequence Viewer

Length: 630 bp
ATGGAAAAAGAAACCCTAAAAATCATCCGAATTGGATTGAAGCCAGTTTTACTCCGATCACCACGATCTCCAAAGTCGTCATGCACATGGCAGTTCATTGTTCGTGCAACTCGTGCGTCTGGCAGTTACTCGTTTGTGCATCTGGTTCTTTCTCTTTCTAATTCATATGACCATCTTATCAAAGTGGATCCTAATAACCGGAGAACTACTTGGCCAGTTGCGGCTAAAATTGTTGGAGACAAAGCAAGTTTTTATCATTGTGGTTTCATTAGCTTGCAAGATACACTGTGGGATGCCAAAGGTCGTCACTATTTCTATGATTGCGATGTTCGTGGTGCTATAGATTTTATTTGGGGCAATGGCCAGTCAATTTATGAGAAATGTCAGATAATGTCTTTGAGCAAATTAATTGGCACTACTGGCTATATTACAGCACAAGGTCGTGAAGGTCCAGACGAAACTACTGGTTTTGTATTCAAAGATTGTTATGTATGTGGATCGGGCACTGCATTTCTTGGGAGAGCATACAGAGACTACTCAAGAGTCTTGTTTTCAGGGACGTATATGGAAAATGTTATTACCCCGGAGGGTTGGTCCCTTTGGACACCGCCACAAATTCAACATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

209

Amino Acids

23.62

Weight (kDa)

9.0

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 73 - 202 7.6e-34 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 221, 608
AclWI GGATC 3 cut(s) 182, 195, 505
AcoI YGGCCR 2 cut(s) 212, 361
AcsI RAATTY 1 cut(s) 615
AgsI TTSAA 3 cut(s) 40, 478, 620
AluBI AGCT 1 cut(s) 273
AluI AGCT 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 231, 525
AlwI GGATC 3 cut(s) 182, 195, 505
AoxI GGCC 2 cut(s) 212, 361
ApoI RAATTY 1 cut(s) 615
AseI ATTAAT 1 cut(s) 407
AspS9I GGNCC 2 cut(s) 449, 594
AsuC2I CCSGG 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 51
AvaII GGWCC 2 cut(s) 449, 594
BaeGI GKGCMC 1 cut(s) 506
BalI TGGCCA 2 cut(s) 214, 363
BamHI GGATCC 1 cut(s) 187
BauI CACGAG 1 cut(s) 111
BccI CCATC 1 cut(s) 180
BcnI CCSGG 1 cut(s) 584
BcoDI GTCTC 2 cut(s) 231, 525
BfmI CTRYAG 1 cut(s) 339
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 1 cut(s) 584
Bme18I GGWCC 2 cut(s) 449, 594
BmgT120I GGNCC 2 cut(s) 449, 594
BmiI GGNNCC 2 cut(s) 189, 596
BmrFI CCNGG 1 cut(s) 584
BmsI GCATC 2 cut(s) 148, 283
BpuEI CTTGAG 1 cut(s) 523
BpuMI CCSGG 1 cut(s) 584
BsaJI CCNNGG 1 cut(s) 582
BsaWI WCCGGW 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 578, 608
Bse1I ACTGG 5 cut(s) 44, 215, 364, 424, 469
Bse3DI GCAATG 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 2 cut(s) 24, 298
BseMI GCAATG 1 cut(s) 364
BseNI ACTGG 5 cut(s) 44, 215, 364, 424, 469
BseSI GKGCMC 1 cut(s) 506
BshFI GGCC 2 cut(s) 214, 363
BsiSI CCGG 2 cut(s) 199, 584
BslFI GGGAC 2 cut(s) 571, 580
BsmAI GTCTC 2 cut(s) 231, 525
BsmFI GGGAC 2 cut(s) 571, 580
BsnI GGCC 2 cut(s) 214, 363
Bsp1286I GDGCHC 1 cut(s) 506
Bsp143I GATC 4 cut(s) 56, 65, 187, 497
BspACI CCGC 2 cut(s) 221, 608
BspANI GGCC 2 cut(s) 214, 363
BspLI GGNNCC 2 cut(s) 189, 596
BspPI GGATC 3 cut(s) 182, 195, 505
BsrDI GCAATG 1 cut(s) 364
BsrI ACTGG 5 cut(s) 44, 215, 364, 424, 469
BssECI CCNNGG 1 cut(s) 582
BssMI GATC 4 cut(s) 56, 65, 187, 497
BssSI CACGAG 1 cut(s) 111
Bst2BI CACGAG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 288
BstAPI GCANNNNNTGC 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 275
BstF5I GGATG 2 cut(s) 24, 298
BstKTI GATC 4 cut(s) 59, 68, 190, 500
BstMAI GTCTC 2 cut(s) 231, 525
BstMBI GATC 4 cut(s) 56, 65, 187, 497
BstMWI GCNNNNNNNGC 2 cut(s) 113, 420
BstNSI RCATGY 1 cut(s) 626
BstSCI CCNGG 1 cut(s) 582
BstSFI CTRYAG 1 cut(s) 339
BstSLI GKGCMC 1 cut(s) 506
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 2 cut(s) 214, 363
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 24, 298
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 284, 504
Cac8I GCNNGC 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 449, 594
CseI GACGC 1 cut(s) 105
CviAII CATG 3 cut(s) 81, 87, 623
CviJI RGCY 6 cut(s) 43, 214, 224, 273, 363, 423
CviKI_1 RGCY 6 cut(s) 43, 214, 224, 273, 363, 423
DpnI GATC 4 cut(s) 58, 67, 189, 499
DpnII GATC 4 cut(s) 56, 65, 187, 497
EaeI YGGCCR 2 cut(s) 212, 361
Eco47I GGWCC 2 cut(s) 449, 594
FaeI CATG 3 cut(s) 84, 90, 626
FaqI GGGAC 2 cut(s) 571, 580
FatI CATG 3 cut(s) 80, 86, 622
FauNDI CATATG 1 cut(s) 166
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 2 cut(s) 11, 305
Fsp4HI GCNGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 222
HaeIII GGCC 2 cut(s) 214, 363
HapII CCGG 2 cut(s) 199, 584
HgaI GACGC 1 cut(s) 105
Hin1II CATG 3 cut(s) 84, 90, 626
HinfI GANTC 1 cut(s) 543
HpaII CCGG 2 cut(s) 199, 584
HphI GGTGA 1 cut(s) 51
Hpy188I TCNGA 3 cut(s) 29, 56, 387
Hpy188III TCNNGA 3 cut(s) 443, 452, 540
HpyAV CCTTC 1 cut(s) 440
HpyCH4III ACNGT 1 cut(s) 288
HpyCH4IV ACGT 1 cut(s) 560
HpyCH4V TGCA 5 cut(s) 84, 107, 139, 277, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 420
HpySE526I ACGT 1 cut(s) 560
Hsp92II CATG 3 cut(s) 84, 90, 626
Kzo9I GATC 4 cut(s) 56, 65, 187, 497
LweI GCATC 2 cut(s) 148, 283
MaeII ACGT 1 cut(s) 560
MaeIII GTNAC 2 cut(s) 125, 305
MalI GATC 4 cut(s) 58, 67, 189, 499
MboI GATC 4 cut(s) 56, 65, 187, 497
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 506
MlsI TGGCCA 2 cut(s) 214, 363
MluCI AATT 7 cut(s) 30, 160, 228, 369, 404, 408, 615
MluNI TGGCCA 2 cut(s) 214, 363
MlyI GAGTC 1 cut(s) 552
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 1 cut(s) 580
Mox20I TGGCCA 2 cut(s) 214, 363
MscI TGGCCA 2 cut(s) 214, 363
MseI TTAA 1 cut(s) 407
MslI CAYNNNNRTG 1 cut(s) 85
Msp20I TGGCCA 2 cut(s) 214, 363
MspI CCGG 2 cut(s) 199, 584
MspR9I CCNGG 1 cut(s) 584
MwoI GCNNNNNNNGC 2 cut(s) 113, 420
NciI CCSGG 1 cut(s) 584
NdeI CATATG 1 cut(s) 166
NdeII GATC 4 cut(s) 56, 65, 187, 497
NlaIII CATG 3 cut(s) 84, 90, 626
NlaIV GGNNCC 2 cut(s) 189, 596
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 1 cut(s) 626
PcsI WCGNNNNNNNCGW 1 cut(s) 109
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 1 cut(s) 551
PpsI GAGTC 1 cut(s) 551
PshBI ATTAAT 1 cut(s) 407
PspN4I GGNNCC 2 cut(s) 189, 596
PspPI GGNCC 2 cut(s) 449, 594
PsuI RGATCY 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 85
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 4 cut(s) 56, 65, 187, 497
Sau96I GGNCC 2 cut(s) 449, 594
SchI GAGTC 1 cut(s) 552
ScrFI CCNGG 1 cut(s) 584
SduI GDGCHC 1 cut(s) 506
SetI ASST 5 cut(s) 275, 304, 442, 451, 563
SfaNI GCATC 2 cut(s) 148, 283
SfcI CTRYAG 1 cut(s) 339
SinI GGWCC 2 cut(s) 449, 594
SmiMI CAYNNNNRTG 1 cut(s) 85
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
Sse9I AATT 7 cut(s) 30, 160, 228, 369, 404, 408, 615
SsiI CCGC 2 cut(s) 221, 608
StyD4I CCNGG 1 cut(s) 582
TaaI ACNGT 1 cut(s) 288
TaiI ACGT 1 cut(s) 563
TasI AATT 7 cut(s) 30, 160, 228, 369, 404, 408, 615
TauI GCSGC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TscAI CASTG 2 cut(s) 291, 511
TseFI GTSAC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 3 cut(s) 85, 153, 256
TspRI CASTG 2 cut(s) 291, 511
VpaK11BI GGWCC 2 cut(s) 449, 594
VspI ATTAAT 1 cut(s) 407
XapI RAATTY 1 cut(s) 615
XceI RCATGY 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.