RchiOBHm_Chr2g0109011

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001788
Physical Location & Seq
Reverse (-)
20411149 .. 20413141
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48287

Sequence Viewer

Length: 1029 bp
ATGGAGTCTCTCATATCAATAATACCATTATTGGCTCTCTTCTATTTCTTCTCTAAATTGGGTACATGTTCTAATGCAATGAAATTTGATCTAAATGATGAGGTATCACATACCATTACAGTGGATATGAAGGGTCGAGGAGATTTTAGAACAGTTCAAGAAGCCATTGATTCCATACCCTCAAATAACGCTTGGTGGATTCGAATCCAACTTAACAATGGTCTCTACTTTGAAAAAGTGCTGATTCCTGAAGACAAACCATACATTATTCTTGAAGGTCACCCTAAATATCTCACTGCAATAGAATTTGGAGACTTTGGCAGTGTTTCAAAGAGTCCTACATTTACGTTACTTGCAGATAATTTCGTAGCTCGGAATATTCTATTCAAGAATTCATATGACCATCTTATCAAAGTGGATCCTAATAACCGGAGAACTACTTGGGCAGTTGCGGCTAAAATTGTTGGAGACAAAGCAAGTTTTTATCATTGTGGTTTCATTAGCTTGCAAGATACACTGTGGGATGCCAAAGGTCGTCACTATTTCTATGATTGCGATGTTCGTGGTGCTATAGATTTTATTTGGGGCAATGGCCAGTCAATTTATGAGAAATGTCAGATAATGTCTTTGAGCAAATTAATTGGCACTACTGGCTATATTACAGCACAAGGTCGTGAAGGTCCAGACGAAACTACTGGTTTTGTATTCAAAGATTGTTATGTATGTGGATCGGGCACTGCATTTCTTGGGAGAGCATACAGAGACTACTCAAGAGTCTTGTTTTCAGGGACGTATATGGAAAATGTTATTACCCCGGAGGGTTGGTCCCTTTGGACACCGCCACAAATTCAACATGCGGATGTGATCACATTCTCAGAGGCGAATTGCAGAGGACCTGGAGCAGATACGTCCCGTCGTATTTGGTGGGAGAAGAAGCTATCAGATGAGGAAGTGATTAATTTAACAAATACAGCTTCTTTCATAGACCAAGATGGCTGGTTGGGAAAGCAACCGCGCGCATTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

342

Amino Acids

38.69

Weight (kDa)

5.59

Isoelectric Point (pI)

27.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 39 - 329 2.3e-56 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1015, 1017
AciI CCGC 4 cut(s) 452, 839, 857, 1013
AclWI GGATC 3 cut(s) 413, 426, 736
AcoI YGGCCR 1 cut(s) 592
AcsI RAATTY 4 cut(s) 83, 305, 391, 846
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 1 cut(s) 64
AflIII ACRYGT 1 cut(s) 65
AgsI TTSAA 7 cut(s) 158, 233, 275, 330, 388, 709, 851
AjnI CCWGG 1 cut(s) 895
AluBI AGCT 4 cut(s) 371, 504, 937, 974
AluI AGCT 4 cut(s) 371, 504, 937, 974
Alw26I GTCTC 5 cut(s) 12, 227, 306, 462, 756
AlwI GGATC 3 cut(s) 413, 426, 736
AoxI GGCC 1 cut(s) 592
ApoI RAATTY 4 cut(s) 83, 305, 391, 846
AseI ATTAAT 2 cut(s) 638, 957
AspLEI GCGC 2 cut(s) 1017, 1019
AspS9I GGNCC 3 cut(s) 680, 825, 893
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 1 cut(s) 272
AsuII TTCGAA 1 cut(s) 202
AvaII GGWCC 3 cut(s) 680, 825, 893
BaeGI GKGCMC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 594
BamHI GGATCC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 258
BccI CCATC 2 cut(s) 411, 986
BciT130I CCWGG 1 cut(s) 897
BclI TGATCA 1 cut(s) 864
BcnI CCSGG 1 cut(s) 815
BcoDI GTCTC 5 cut(s) 12, 227, 306, 462, 756
BfmI CTRYAG 1 cut(s) 570
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 2 cut(s) 815, 897
Bme18I GGWCC 3 cut(s) 680, 825, 893
BmgT120I GGNCC 3 cut(s) 680, 825, 893
BmiI GGNNCC 2 cut(s) 420, 827
BmrFI CCNGG 2 cut(s) 815, 897
BmsI GCATC 1 cut(s) 514
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 918
Bpu14I TTCGAA 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 754
BpuMI CCSGG 1 cut(s) 815
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 813
BsaWI WCCGGW 1 cut(s) 429
BsaXI ACNNNNNCTCC 2 cut(s) 809, 839
Bse1I ACTGG 3 cut(s) 595, 655, 700
Bse3DI GCAATG 2 cut(s) 84, 595
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 1 cut(s) 897
BseDI CCNNGG 1 cut(s) 813
BseGI GGATG 2 cut(s) 529, 865
BseJI GATNNNNATC 1 cut(s) 203
BseMI GCAATG 2 cut(s) 84, 595
BseMII CTCAG 1 cut(s) 888
BseNI ACTGG 3 cut(s) 595, 655, 700
BsePI GCGCGC 1 cut(s) 1015
BseRI GAGGAG 1 cut(s) 153
BseSI GKGCMC 1 cut(s) 737
Bsh1236I CGCG 2 cut(s) 1015, 1017
BshFI GGCC 1 cut(s) 594
BsiSI CCGG 2 cut(s) 430, 815
BslFI GGGAC 3 cut(s) 802, 811, 895
BsmAI GTCTC 5 cut(s) 12, 227, 306, 462, 756
BsmFI GGGAC 3 cut(s) 802, 811, 895
BsnI GGCC 1 cut(s) 594
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 737
Bsp143I GATC 4 cut(s) 88, 418, 728, 864
BspACI CCGC 4 cut(s) 452, 839, 857, 1013
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 1 cut(s) 887
BspFNI CGCG 2 cut(s) 1015, 1017
BspLI GGNNCC 2 cut(s) 420, 827
BspPI GGATC 3 cut(s) 413, 426, 736
BspT104I TTCGAA 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 2 cut(s) 84, 595
BsrI ACTGG 3 cut(s) 595, 655, 700
BssECI CCNNGG 1 cut(s) 813
BssHII GCGCGC 1 cut(s) 1015
BssMI GATC 4 cut(s) 88, 418, 728, 864
Bst2UI CCWGG 1 cut(s) 897
Bst4CI ACNGT 3 cut(s) 121, 154, 519
Bst6I CTCTTC 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 506, 1017
BstDEI CTNAG 1 cut(s) 874
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 2 cut(s) 529, 865
BstFNI CGCG 2 cut(s) 1015, 1017
BstHHI GCGC 2 cut(s) 1017, 1019
BstKTI GATC 4 cut(s) 91, 421, 731, 867
BstMAI GTCTC 5 cut(s) 12, 227, 306, 462, 756
BstMBI GATC 4 cut(s) 88, 418, 728, 864
BstMWI GCNNNNNNNGC 2 cut(s) 452, 651
BstNI CCWGG 1 cut(s) 897
BstNSI RCATGY 2 cut(s) 69, 857
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 813, 895
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 1 cut(s) 737
BstUI CGCG 2 cut(s) 1015, 1017
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 1 cut(s) 418
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 1 cut(s) 594
BtgZI GCGATG 1 cut(s) 570
BtsCI GGATG 2 cut(s) 529, 865
BtsI GCAGTG 3 cut(s) 294, 328, 735
BtsIMutI CAGTG 5 cut(s) 126, 294, 328, 515, 735
Cac8I GCNNGC 2 cut(s) 506, 1017
CfoI GCGC 2 cut(s) 1017, 1019
Cfr13I GGNCC 3 cut(s) 680, 825, 893
Csp6I GTAC 1 cut(s) 63
CviAII CATG 2 cut(s) 66, 854
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 1 cut(s) 874
DpnI GATC 4 cut(s) 90, 420, 730, 866
DpnII GATC 4 cut(s) 88, 418, 728, 864
EaeI YGGCCR 1 cut(s) 592
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 3 cut(s) 680, 825, 893
Eco57I CTGAAG 1 cut(s) 270
Eco91I GGTNACC 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 893
EcoO65I GGTNACC 1 cut(s) 278
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 895
FaeI CATG 2 cut(s) 69, 857
FaqI GGGAC 3 cut(s) 802, 811, 895
FatI CATG 2 cut(s) 65, 853
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 864
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 2 cut(s) 536, 872
Fsp4HI GCNGC 1 cut(s) 453
GlaI GCGC 2 cut(s) 1016, 1018
GluI GCNGC 1 cut(s) 453
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 1 cut(s) 594
HapII CCGG 2 cut(s) 430, 815
HhaI GCGC 2 cut(s) 1017, 1019
Hin1II CATG 2 cut(s) 69, 857
Hin6I GCGC 2 cut(s) 1015, 1017
HinP1I GCGC 2 cut(s) 1015, 1017
HinfI GANTC 7 cut(s) 5, 170, 199, 204, 244, 334, 774
HpaII CCGG 2 cut(s) 430, 815
HphI GGTGA 1 cut(s) 272
Hpy188I TCNGA 4 cut(s) 375, 618, 877, 943
Hpy188III TCNNGA 7 cut(s) 158, 248, 272, 388, 674, 683, 771
Hpy99I CGWCG 1 cut(s) 918
HpyAV CCTTC 3 cut(s) 124, 269, 671
HpyCH4III ACNGT 3 cut(s) 121, 154, 519
HpyCH4IV ACGT 3 cut(s) 347, 791, 908
HpyCH4V TGCA 6 cut(s) 77, 299, 356, 508, 740, 888
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 651
HpyF3I CTNAG 1 cut(s) 874
HpySE526I ACGT 3 cut(s) 347, 791, 908
Hsp92II CATG 2 cut(s) 69, 857
HspAI GCGC 2 cut(s) 1015, 1017
Ksp22I TGATCA 1 cut(s) 864
Kzo9I GATC 4 cut(s) 88, 418, 728, 864
LmnI GCTCC 1 cut(s) 899
LweI GCATC 1 cut(s) 514
MaeII ACGT 3 cut(s) 347, 791, 908
MaeIII GTNAC 3 cut(s) 278, 348, 536
MalI GATC 4 cut(s) 90, 420, 730, 866
MboI GATC 4 cut(s) 88, 418, 728, 864
MboII GAAGA 4 cut(s) 31, 40, 263, 943
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 1 cut(s) 737
MlsI TGGCCA 1 cut(s) 594
MluNI TGGCCA 1 cut(s) 594
MlyI GAGTC 3 cut(s) 14, 343, 783
MmeI TCCRAC 2 cut(s) 232, 445
MnlI CCTC 7 cut(s) 94, 131, 190, 811, 871, 884, 940
Mox20I TGGCCA 1 cut(s) 594
MscI TGGCCA 1 cut(s) 594
MseI TTAA 5 cut(s) 213, 638, 957, 962, 1022
MslI CAYNNNNRTG 2 cut(s) 119, 858
Msp20I TGGCCA 1 cut(s) 594
MspI CCGG 2 cut(s) 430, 815
MspR9I CCNGG 2 cut(s) 815, 897
MvaI CCWGG 1 cut(s) 897
MvnI CGCG 2 cut(s) 1015, 1017
MwoI GCNNNNNNNGC 2 cut(s) 452, 651
NciI CCSGG 1 cut(s) 815
NdeI CATATG 1 cut(s) 397
NdeII GATC 4 cut(s) 88, 418, 728, 864
NlaIII CATG 2 cut(s) 69, 857
NlaIV GGNNCC 2 cut(s) 420, 827
NmuCI GTSAC 2 cut(s) 278, 536
NspI RCATGY 2 cut(s) 69, 857
NspV TTCGAA 1 cut(s) 202
PauI GCGCGC 1 cut(s) 1015
PciI ACATGT 1 cut(s) 65
PfeI GAWTC 4 cut(s) 170, 199, 204, 244
PkrI GCNGC 1 cut(s) 454
PleI GAGTC 3 cut(s) 13, 342, 782
PpsI GAGTC 3 cut(s) 13, 342, 782
PpuMI RGGWCCY 1 cut(s) 893
PscI ACATGT 1 cut(s) 65
PshBI ATTAAT 2 cut(s) 638, 957
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 1 cut(s) 895
PspEI GGTNACC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 895
PspN4I GGNNCC 2 cut(s) 420, 827
PspPI GGNCC 3 cut(s) 680, 825, 893
PspPPI RGGWCCY 1 cut(s) 893
PsuI RGATCY 1 cut(s) 418
PteI GCGCGC 1 cut(s) 1015
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
RseI CAYNNNNRTG 2 cut(s) 119, 858
SaqAI TTAA 5 cut(s) 213, 638, 957, 962, 1022
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 4 cut(s) 88, 418, 728, 864
Sau96I GGNCC 3 cut(s) 680, 825, 893
SchI GAGTC 3 cut(s) 14, 343, 783
ScrFI CCNGG 2 cut(s) 815, 897
SduI GDGCHC 1 cut(s) 737
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 570
SfuI TTCGAA 1 cut(s) 202
SinI GGWCC 3 cut(s) 680, 825, 893
SmiMI CAYNNNNRTG 2 cut(s) 119, 858
SmlI CTYRAG 1 cut(s) 769
SmoI CTYRAG 1 cut(s) 769
SsiI CCGC 4 cut(s) 452, 839, 857, 1013
SspI AATATT 1 cut(s) 379
StyD4I CCNGG 2 cut(s) 813, 895
TaaI ACNGT 3 cut(s) 121, 154, 519
TaiI ACGT 3 cut(s) 350, 794, 911
TaqI TCGA 2 cut(s) 136, 202
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 4 cut(s) 170, 199, 204, 244
Tru1I TTAA 5 cut(s) 213, 638, 957, 962, 1022
Tru9I TTAA 5 cut(s) 213, 638, 957, 962, 1022
TscAI CASTG 5 cut(s) 126, 301, 328, 522, 742
TseFI GTSAC 2 cut(s) 278, 536
Tsp45I GTSAC 2 cut(s) 278, 536
TspDTI ATGAA 5 cut(s) 95, 143, 384, 487, 970
TspRI CASTG 5 cut(s) 126, 301, 328, 522, 742
VpaK11BI GGWCC 3 cut(s) 680, 825, 893
VspI ATTAAT 2 cut(s) 638, 957
XapI RAATTY 4 cut(s) 83, 305, 391, 846
XceI RCATGY 2 cut(s) 69, 857
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.