AT2G47280

pectinesterase 66

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001788
Physical Location & Seq
Forward (+)
19416775 .. 19418383
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G47280.1

Sequence Viewer

Length: 1011 bp
ATGAAGAGGGTAGCATCTTACAATTACAAAATTGTGTGTATGGTCGTAATGACGTTGTTTGTGTACGGATATTCAGCCGCAGCCGAACAAATAGCTTACACCATAACTGTTGATCTTAACGGTGGCGGAAATTTTACGACCGTCCAAAGTGCAATCGATTCAATCTCTCCTCCCAACCATAATTGGATTCGTGTCTTCACACAAAATGGAATTTACCGGGAGAAAGTAACGATACCAAAAGAAAAAGGATTCATATATTTGCAAGGTAAAGGAATTGAACAAACGGTAATTGAGTACGATGATCATCAAGCCACCGATATCAGCGCAACGTTCACTGCTTTTGCTGATGATATCGTCATCTCCGGTATAACTTTCAAGAACACGTACAATATTGTGCCAAATAACAAACGGGAGATTGTACCGGCGGTGGCGGCGAGGATGCTTGGAGATAGATATGTGGTGACCGATTCTAGTTTCGTCGGACTTCAAGATACTTTATTTGACGGAAAAGGCAGACATTATTACAAGCGATGTATAATTTCTGGCGGGATCGATTTCATATTTGGTTATGGTCAATCACTATTCAAGGAATGTACATTGAATATGACGCTGGGGATATATGCACCGGATAACCCGTATGGAACGATAACTGCGCATCAAAGGCCGTCACCGTCAGATGAGGGAGGATTTGTGTTCAGTGACTGTACCGTTACGGGAGTCGGGAAAACGTTGTTGGGACGAGCATGGGGATCAAACGCACGTGTCATCTTCGACCGTTCACGATTATCCGACGTCGTTCTTCCTATTGGTTGGGATGCTTGGCGTGCAAAAGGCAACGAGAGAGACTTAACGTTTGTGGAGGCTGGTTGCACCGGAGCTGGAGCGGACACGTCACAGAGAGTGCCGTGGCTTAAGAAGCTGAGTCTAAGTGAAGTTGATGGATTCGCCAGCGTTTCCTTCATTGACCAAGATGGTTGGATCTCTAGGTTCCCCATTGAGGGTGGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

336

Amino Acids

37.02

Weight (kDa)

5.86

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 35 - 323 2.7e-51 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 795
Acc16I TGCGCA 1 cut(s) 654
AccBSI CCGCTC 1 cut(s) 884
AciI CCGC 6 cut(s) 78, 126, 425, 431, 546, 884
AclI AACGTT 3 cut(s) 329, 728, 851
AclWI GGATC 3 cut(s) 557, 757, 986
AcsI RAATTY 2 cut(s) 130, 210
AcvI CACGTG 1 cut(s) 761
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 6 cut(s) 65, 296, 386, 420, 595, 706
AfiI CCNNNNNNNGG 2 cut(s) 997, 998
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 3 cut(s) 381, 760, 888
AgsI TTSAA 6 cut(s) 162, 278, 376, 488, 586, 601
AjiI CACGTC 1 cut(s) 891
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AluBI AGCT 3 cut(s) 95, 878, 919
AluI AGCT 3 cut(s) 95, 878, 919
Alw26I GTCTC 1 cut(s) 837
AlwI GGATC 3 cut(s) 557, 757, 986
AlwNI CAGNNNCTG 1 cut(s) 702
AoxI GGCC 2 cut(s) 662, 1004
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 2 cut(s) 130, 210
ArsI GACNNNNNNTTYG 2 cut(s) 836, 868
AspLEI GCGC 2 cut(s) 326, 655
AspS9I GGNCC 1 cut(s) 1004
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 472, 660
BbrPI CACGTG 1 cut(s) 761
BbsI GAAGAC 1 cut(s) 187
BbvI GCAGC 1 cut(s) 92
BccI CCATC 2 cut(s) 932, 965
BceAI ACGGC 2 cut(s) 649, 889
BclI TGATCA 1 cut(s) 301
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 1 cut(s) 837
BfaI CTAG 2 cut(s) 471, 984
BfrI CTTAAG 1 cut(s) 911
BisI GCNGC 3 cut(s) 78, 81, 432
BlsI GCNGC 3 cut(s) 79, 82, 433
Bme1390I CCNGG 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 891
BmgT120I GGNCC 1 cut(s) 1004
BmiI GGNNCC 1 cut(s) 989
BmrFI CCNGG 1 cut(s) 218
BmsI GCATC 4 cut(s) 23, 429, 664, 805
BpiI GAAGAC 1 cut(s) 187
BpmI CTGGAG 1 cut(s) 900
BpuMI CCSGG 1 cut(s) 218
Bsa29I ATCGAT 2 cut(s) 156, 552
BsaAI YACGTR 2 cut(s) 384, 761
BsaBI GATNNNNATC 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 1 cut(s) 905
BsaWI WCCGGW 3 cut(s) 362, 625, 872
BsaXI ACNNNNNCTCC 2 cut(s) 675, 705
Bsc4I CCNNNNNNNGG 2 cut(s) 997, 998
Bse118I RCCGGY 1 cut(s) 421
Bse8I GATNNNNATC 1 cut(s) 303
BseCI ATCGAT 2 cut(s) 156, 552
BseDI CCNNGG 1 cut(s) 905
BseGI GGATG 2 cut(s) 444, 820
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 2 cut(s) 997, 998
BseMII CTCAG 1 cut(s) 911
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 92
BseYI CCCAGC 1 cut(s) 610
Bsh1285I CGRYCG 2 cut(s) 141, 775
BshFI GGCC 2 cut(s) 664, 1006
BshVI ATCGAT 2 cut(s) 156, 552
BsiEI CGRYCG 2 cut(s) 141, 775
BsiSI CCGG 5 cut(s) 217, 363, 422, 626, 873
BslFI GGGAC 1 cut(s) 750
BslI CCNNNNNNNGG 2 cut(s) 997, 998
BsmAI GTCTC 1 cut(s) 837
BsmFI GGGAC 1 cut(s) 750
BsnI GGCC 2 cut(s) 664, 1006
Bsp1407I TGTACA 1 cut(s) 593
Bsp143I GATC 5 cut(s) 112, 301, 549, 749, 978
BspACI CCGC 6 cut(s) 78, 126, 425, 431, 546, 884
BspANI GGCC 2 cut(s) 664, 1006
BspCNI CTCAG 1 cut(s) 912
BspDI ATCGAT 2 cut(s) 156, 552
BspLI GGNNCC 1 cut(s) 989
BspPI GGATC 3 cut(s) 557, 757, 986
BspTI CTTAAG 1 cut(s) 911
BsrBI CCGCTC 1 cut(s) 884
BsrFI RCCGGY 1 cut(s) 421
BsrGI TGTACA 1 cut(s) 593
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 905
BssMI GATC 5 cut(s) 112, 301, 549, 749, 978
BssNI GRCGYC 1 cut(s) 792
Bst4CI ACNGT 8 cut(s) 109, 122, 142, 286, 672, 704, 709, 776
BstACI GRCGYC 1 cut(s) 792
BstAFI CTTAAG 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 593
BstBAI YACGTR 2 cut(s) 384, 761
BstC8I GCNNGC 2 cut(s) 825, 949
BstDEI CTNAG 2 cut(s) 920, 926
BstDSI CCRYGG 1 cut(s) 905
BstEII GGTNACC 1 cut(s) 460
BstF5I GGATG 2 cut(s) 444, 820
BstHHI GCGC 2 cut(s) 326, 655
BstKTI GATC 5 cut(s) 115, 304, 552, 752, 981
BstMAI GTCTC 1 cut(s) 837
BstMBI GATC 5 cut(s) 112, 301, 549, 749, 978
BstMCI CGRYCG 2 cut(s) 141, 775
BstMWI GCNNNNNNNGC 4 cut(s) 431, 661, 824, 916
BstPI GGTNACC 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 92
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 978
BstYI RGATCY 1 cut(s) 978
Bsu15I ATCGAT 2 cut(s) 156, 552
BsuRI GGCC 2 cut(s) 664, 1006
BsuTUI ATCGAT 2 cut(s) 156, 552
BtgI CCRYGG 1 cut(s) 905
BtgZI GCGATG 1 cut(s) 544
BtrI CACGTC 1 cut(s) 891
BtsCI GGATG 2 cut(s) 444, 820
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 2 cut(s) 333, 703
Cac8I GCNNGC 2 cut(s) 825, 949
CaiI CAGNNNCTG 1 cut(s) 702
CfoI GCGC 2 cut(s) 326, 655
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 1004
ClaI ATCGAT 2 cut(s) 156, 552
CseI GACGC 1 cut(s) 616
Csp6I GTAC 6 cut(s) 64, 295, 385, 419, 594, 705
CviAII CATG 1 cut(s) 744
CviQI GTAC 6 cut(s) 64, 295, 385, 419, 594, 705
DdeI CTNAG 2 cut(s) 920, 926
DpnI GATC 5 cut(s) 114, 303, 551, 751, 980
DpnII GATC 5 cut(s) 112, 301, 549, 749, 978
EciI GGCGGA 1 cut(s) 141
Eco32I GATATC 2 cut(s) 319, 352
Eco72I CACGTG 1 cut(s) 761
Eco91I GGTNACC 1 cut(s) 460
EcoO65I GGTNACC 1 cut(s) 460
EcoRV GATATC 2 cut(s) 319, 352
FaeI CATG 1 cut(s) 747
FaqI GGGAC 1 cut(s) 750
FatI CATG 1 cut(s) 743
FauI CCCGC 1 cut(s) 539
FbaI TGATCA 1 cut(s) 301
Fnu4HI GCNGC 3 cut(s) 78, 81, 432
FokI GGATG 2 cut(s) 451, 827
Fsp4HI GCNGC 3 cut(s) 78, 81, 432
FspBI CTAG 2 cut(s) 471, 984
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 2 cut(s) 325, 654
GluI GCNGC 3 cut(s) 78, 81, 432
GsaI CCCAGC 1 cut(s) 614
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 2 cut(s) 664, 1006
HapII CCGG 5 cut(s) 217, 363, 422, 626, 873
HgaI GACGC 1 cut(s) 616
HhaI GCGC 2 cut(s) 326, 655
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 1 cut(s) 747
Hin6I GCGC 2 cut(s) 324, 653
HinP1I GCGC 2 cut(s) 324, 653
HinfI GANTC 7 cut(s) 158, 187, 249, 467, 717, 922, 942
HpaII CCGG 5 cut(s) 217, 363, 422, 626, 873
HphI GGTGA 2 cut(s) 472, 660
Hpy166II GTNNAC 3 cut(s) 64, 333, 779
Hpy188I TCNGA 3 cut(s) 482, 676, 790
Hpy188III TCNNGA 4 cut(s) 376, 488, 721, 780
Hpy8I GTNNAC 3 cut(s) 64, 333, 779
Hpy99I CGWCG 3 cut(s) 482, 794, 797
HpyAV CCTTC 1 cut(s) 967
HpyCH4III ACNGT 8 cut(s) 109, 122, 142, 286, 672, 704, 709, 776
HpyCH4IV ACGT 8 cut(s) 53, 329, 383, 728, 760, 792, 851, 890
HpyCH4V TGCA 5 cut(s) 152, 262, 623, 827, 870
HpyF10VI GCNNNNNNNGC 4 cut(s) 431, 661, 824, 916
HpyF3I CTNAG 2 cut(s) 920, 926
HpySE526I ACGT 8 cut(s) 53, 329, 383, 728, 760, 792, 851, 890
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 1 cut(s) 747
HspAI GCGC 2 cut(s) 324, 653
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 5 cut(s) 112, 301, 549, 749, 978
LmnI GCTCC 2 cut(s) 875, 881
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 4 cut(s) 23, 429, 664, 805
MaeI CTAG 2 cut(s) 471, 984
MaeII ACGT 8 cut(s) 53, 329, 383, 728, 760, 792, 851, 890
MaeIII GTNAC 6 cut(s) 226, 460, 666, 698, 709, 891
MalI GATC 5 cut(s) 114, 303, 551, 751, 980
MbiI CCGCTC 1 cut(s) 884
MboI GATC 5 cut(s) 112, 301, 549, 749, 978
MboII GAAGA 4 cut(s) 16, 187, 760, 791
MflI RGATCY 1 cut(s) 978
MluCI AATT 8 cut(s) 22, 30, 130, 181, 210, 273, 288, 537
MlyI GAGTC 2 cut(s) 726, 931
MmeI TCCRAC 3 cut(s) 460, 813, 956
MnlI CCTC 6 cut(s) 180, 429, 673, 677, 853, 991
MseI TTAA 4 cut(s) 117, 848, 912, 1009
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 5 cut(s) 217, 363, 422, 626, 873
MspR9I CCNGG 1 cut(s) 218
MwoI GCNNNNNNNGC 4 cut(s) 431, 661, 824, 916
NciI CCSGG 1 cut(s) 218
NdeII GATC 5 cut(s) 112, 301, 549, 749, 978
NlaIII CATG 1 cut(s) 747
NlaIV GGNNCC 1 cut(s) 989
NmuCI GTSAC 4 cut(s) 460, 666, 698, 891
NsbI TGCGCA 1 cut(s) 654
PfeI GAWTC 5 cut(s) 158, 187, 249, 467, 942
PkrI GCNGC 3 cut(s) 79, 82, 433
PleI GAGTC 2 cut(s) 725, 930
PmaCI CACGTG 1 cut(s) 761
PmlI CACGTG 1 cut(s) 761
PpsI GAGTC 2 cut(s) 725, 930
Ppu21I YACGTR 2 cut(s) 384, 761
Psp1406I AACGTT 3 cut(s) 329, 728, 851
PspCI CACGTG 1 cut(s) 761
PspEI GGTNACC 1 cut(s) 460
PspFI CCCAGC 1 cut(s) 610
PspN4I GGNNCC 1 cut(s) 989
PspPI GGNCC 1 cut(s) 1004
PstNI CAGNNNCTG 1 cut(s) 702
PsuI RGATCY 1 cut(s) 978
RsaI GTAC 6 cut(s) 65, 296, 386, 420, 595, 706
RsaNI GTAC 6 cut(s) 64, 295, 385, 419, 594, 705
SaqAI TTAA 4 cut(s) 117, 848, 912, 1009
SatI GCNGC 3 cut(s) 78, 81, 432
Sau3AI GATC 5 cut(s) 112, 301, 549, 749, 978
Sau96I GGNCC 1 cut(s) 1004
SchI GAGTC 2 cut(s) 726, 931
ScrFI CCNGG 1 cut(s) 218
SfaNI GCATC 4 cut(s) 23, 429, 664, 805
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
Sse9I AATT 8 cut(s) 22, 30, 130, 181, 210, 273, 288, 537
SsiI CCGC 6 cut(s) 78, 126, 425, 431, 546, 884
SspI AATATT 1 cut(s) 391
SspMI CTAG 2 cut(s) 471, 984
StyD4I CCNGG 1 cut(s) 216
TaaI ACNGT 8 cut(s) 109, 122, 142, 286, 672, 704, 709, 776
TaiI ACGT 8 cut(s) 56, 332, 386, 731, 763, 795, 854, 893
TaqI TCGA 3 cut(s) 156, 552, 771
TaqII GACCGA 1 cut(s) 479
TasI AATT 8 cut(s) 22, 30, 130, 181, 210, 273, 288, 537
TatI WGTACW 1 cut(s) 593
TauI GCSGC 2 cut(s) 80, 434
TfiI GAWTC 5 cut(s) 158, 187, 249, 467, 942
Tru1I TTAA 4 cut(s) 117, 848, 912, 1009
Tru9I TTAA 4 cut(s) 117, 848, 912, 1009
TscAI CASTG 2 cut(s) 340, 703
TseFI GTSAC 4 cut(s) 460, 666, 698, 891
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 4 cut(s) 460, 666, 698, 891
TspDTI ATGAA 4 cut(s) 17, 241, 547, 949
TspGWI ACGGA 2 cut(s) 81, 519
TspRI CASTG 2 cut(s) 340, 703
Vha464I CTTAAG 1 cut(s) 911
XapI RAATTY 2 cut(s) 130, 210
XspI CTAG 2 cut(s) 471, 984
ZraI GACGTC 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.