Rroxscaffold_6G00422090

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001788
Physical Location & Seq
Forward (+)
43091834 .. 43092932
1099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422090.1

Sequence Viewer

Length: 618 bp
ATGCAGTCGTTTATATCAATAACGTTGGCCCTCTTCTTTGTCTTTGGTGCATTGGATGCTCTCCCTTATCCACTTAAATATGATCTTCCACAAGTTGTTTCAAGAACCATTATAGTTGATAAGCATGGCCGAGGTGATTTTGCCTCGGTGCAACAAGCCATTGATTCTATAATCAACTCCGGCGTCTACGTGTTATACGGTGGCAATGGAAGGAAAGTAGCCCAAGCACCCGCAGCTGCAATATATGGAGACAAAGCAAGTTTTTATCGGTGTAGTTTCCACAGCGTGCAAGATACACTGGCCGATATTCTAGGTCGTCACTATTTTTATAATTGCTATATTGAAGGTCATGTAGACTTTATTTGGGGCTTCGGCCAGTCCATTTATGAGAAATGTCAATTAAATTCACTTGGTTCCGGCTATATCACAGCGCAGGGTCGTGATGGCGCAAACAACCCTACTGGTTTCGTATTCAAAGATTGTCATGTATCCGGATCAGGTCCTACACTGCTGGGAAGAGCATACAGGCCATACGCTAGAGTGTTGTTTGTTGGAATATATATGGAAGATATGATTAAGCCAGAAGGTTGGGACGCACCATGGAACAAGGGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

205

Amino Acids

22.71

Weight (kDa)

7.67

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 70 - 203 1.3e-27 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
AccI GTMKAC 2 cut(s) 186, 354
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 1 cut(s) 231
AclI AACGTT 1 cut(s) 23
AclWI GGATC 1 cut(s) 502
AcoI YGGCCR 3 cut(s) 127, 300, 373
AcsI RAATTY 1 cut(s) 403
AcyI GRCGYC 1 cut(s) 183
AdeI CACNNNGTG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 3 cut(s) 102, 344, 475
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 502
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 5 cut(s) 27, 127, 300, 373, 527
ApeKI GCWGC 2 cut(s) 233, 236
ApoI RAATTY 1 cut(s) 403
AspLEI GCGC 2 cut(s) 433, 449
AspS9I GGNCC 2 cut(s) 28, 500
AsuHPI GGTGA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 500
BbvI GCAGC 2 cut(s) 223, 245
BccI CCATC 1 cut(s) 437
BciVI GTATCC 1 cut(s) 499
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 2 cut(s) 311, 537
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 2 cut(s) 234, 237
BlsI GCNGC 2 cut(s) 235, 238
Bme18I GGWCC 1 cut(s) 500
BmgT120I GGNCC 2 cut(s) 28, 500
BmiI GGNNCC 1 cut(s) 415
BmsI GCATC 1 cut(s) 46
BsaAI YACGTR 1 cut(s) 190
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 3 cut(s) 130, 144, 599
BsaWI WCCGGW 1 cut(s) 491
Bse1I ACTGG 3 cut(s) 303, 376, 466
Bse3DI GCAATG 1 cut(s) 211
BseAI TCCGGA 1 cut(s) 491
BseDI CCNNGG 3 cut(s) 130, 144, 599
BseGI GGATG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 211
BseNI ACTGG 3 cut(s) 303, 376, 466
BseXI GCAGC 2 cut(s) 223, 245
BseYI CCCAGC 1 cut(s) 511
BshFI GGCC 5 cut(s) 29, 129, 302, 375, 529
BsiSI CCGG 3 cut(s) 180, 417, 492
BslFI GGGAC 1 cut(s) 605
BsmAI GTCTC 1 cut(s) 243
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 5 cut(s) 29, 129, 302, 375, 529
Bsp13I TCCGGA 1 cut(s) 491
Bsp143I GATC 2 cut(s) 82, 494
Bsp19I CCATGG 1 cut(s) 599
BspACI CCGC 1 cut(s) 231
BspANI GGCC 5 cut(s) 29, 129, 302, 375, 529
BspEI TCCGGA 1 cut(s) 491
BspLI GGNNCC 1 cut(s) 415
BspPI GGATC 1 cut(s) 502
BspQI GCTCTTC 1 cut(s) 511
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 3 cut(s) 303, 376, 466
BssECI CCNNGG 3 cut(s) 130, 144, 599
BssMI GATC 2 cut(s) 82, 494
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 599
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 2 cut(s) 38, 511
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 56
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 599
BstF5I GGATG 1 cut(s) 61
BstHHI GCGC 2 cut(s) 433, 449
BstKTI GATC 2 cut(s) 85, 497
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 2 cut(s) 82, 494
BstMWI GCNNNNNNNGC 2 cut(s) 56, 233
BstV1I GCAGC 2 cut(s) 223, 245
BstXI CCANNNNNNTGG 1 cut(s) 588
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 5 cut(s) 29, 129, 302, 375, 529
BtgI CCRYGG 1 cut(s) 599
BtsCI GGATG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 506
BtsIMutI CAGTG 2 cut(s) 296, 506
Cac8I GCNNGC 1 cut(s) 287
CfoI GCGC 2 cut(s) 433, 449
Cfr13I GGNCC 2 cut(s) 28, 500
CseI GACGC 2 cut(s) 172, 602
CviAII CATG 4 cut(s) 125, 350, 485, 600
DpnI GATC 2 cut(s) 84, 496
DpnII GATC 2 cut(s) 82, 494
DraIII CACNNNGTG 1 cut(s) 286
EaeI YGGCCR 3 cut(s) 127, 300, 373
Eam1104I CTCTTC 2 cut(s) 38, 511
EarI CTCTTC 2 cut(s) 38, 511
Eco130I CCWWGG 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 500
EcoO109I RGGNCCY 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 599
ErhI CCWWGG 1 cut(s) 599
FaeI CATG 4 cut(s) 128, 353, 488, 603
FaqI GGGAC 1 cut(s) 605
FatI CATG 4 cut(s) 124, 349, 484, 599
FauI CCCGC 1 cut(s) 238
FblI GTMKAC 2 cut(s) 186, 354
Fnu4HI GCNGC 2 cut(s) 234, 237
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 234, 237
FspBI CTAG 2 cut(s) 311, 537
GlaI GCGC 2 cut(s) 432, 448
GluI GCNGC 2 cut(s) 234, 237
GsaI CCCAGC 1 cut(s) 515
HaeIII GGCC 5 cut(s) 29, 129, 302, 375, 529
HapII CCGG 3 cut(s) 180, 417, 492
HgaI GACGC 2 cut(s) 172, 602
HhaI GCGC 2 cut(s) 433, 449
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 4 cut(s) 128, 353, 488, 603
Hin6I GCGC 2 cut(s) 431, 447
HinP1I GCGC 2 cut(s) 431, 447
HinfI GANTC 1 cut(s) 164
HpaII CCGG 3 cut(s) 180, 417, 492
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 187, 355
Hpy188III TCNNGA 3 cut(s) 102, 440, 492
Hpy8I GTNNAC 2 cut(s) 187, 355
HpyAV CCTTC 3 cut(s) 204, 338, 578
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 2 cut(s) 23, 189
HpyCH4V TGCA 5 cut(s) 4, 50, 151, 239, 289
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 233
HpySE526I ACGT 2 cut(s) 23, 189
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 4 cut(s) 128, 353, 488, 603
HspAI GCGC 2 cut(s) 431, 447
Kpn2I TCCGGA 1 cut(s) 491
Kzo9I GATC 2 cut(s) 82, 494
LguI GCTCTTC 1 cut(s) 511
Lsp1109I GCAGC 2 cut(s) 223, 245
LweI GCATC 1 cut(s) 46
MaeI CTAG 2 cut(s) 311, 537
MaeII ACGT 2 cut(s) 23, 189
MaeIII GTNAC 1 cut(s) 317
MalI GATC 2 cut(s) 84, 496
MboI GATC 2 cut(s) 82, 494
MboII GAAGA 4 cut(s) 25, 77, 528, 578
MluCI AATT 3 cut(s) 331, 398, 403
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 3 cut(s) 41, 125, 154
MroI TCCGGA 1 cut(s) 491
MseI TTAA 3 cut(s) 75, 401, 576
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 180, 417, 492
MwoI GCNNNNNNNGC 2 cut(s) 56, 233
NcoI CCATGG 1 cut(s) 599
NdeII GATC 2 cut(s) 82, 494
NlaIII CATG 4 cut(s) 128, 353, 488, 603
NlaIV GGNNCC 1 cut(s) 415
NmeAIII GCCGAG 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 511
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 2 cut(s) 235, 238
Ppu21I YACGTR 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 500
PsiI TTATAA 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 500
PspFI CCCAGC 1 cut(s) 511
PspN4I GGNNCC 1 cut(s) 415
PspPI GGNCC 2 cut(s) 28, 500
PspPPI RGGWCCY 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 511
SaqAI TTAA 3 cut(s) 75, 401, 576
SatI GCNGC 2 cut(s) 234, 237
Sau3AI GATC 2 cut(s) 82, 494
Sau96I GGNCC 2 cut(s) 28, 500
SetI ASST 8 cut(s) 26, 136, 192, 238, 316, 349, 502, 589
SfaNI GCATC 1 cut(s) 46
SinI GGWCC 1 cut(s) 500
Sse9I AATT 3 cut(s) 331, 398, 403
SsiI CCGC 1 cut(s) 231
SspMI CTAG 2 cut(s) 311, 537
StyI CCWWGG 1 cut(s) 599
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 2 cut(s) 26, 192
TaqI TCGA 1 cut(s) 613
TasI AATT 3 cut(s) 331, 398, 403
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 3 cut(s) 75, 401, 576
Tru9I TTAA 3 cut(s) 75, 401, 576
TscAI CASTG 2 cut(s) 303, 513
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 2 cut(s) 233, 236
Tsp45I GTSAC 1 cut(s) 317
TspRI CASTG 2 cut(s) 303, 513
VpaK11BI GGWCC 1 cut(s) 500
XapI RAATTY 1 cut(s) 403
XmiI GTMKAC 2 cut(s) 186, 354
XspI CTAG 2 cut(s) 311, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.