Prupe.6G312200_v2.0.a1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001788
Physical Location & Seq
Forward (+)
27961433 .. 27962794
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G312200.1

Sequence Viewer

Length: 900 bp
ATGCAGTCCCTTGTAGCATCAATCTTGGCTCTCTTCTTTTTGTTGGGTGCATTGGAAGCTCGCTATAATCTTCAAGACGATGTCGTCGTAAGCGCCGCCATCATAGTCGATCAGAATGGCCATGGAAATTTTACCACAGTCCAGCAAGCCATTGATTCCATCCCTTCAAATAACTCACAGTGGACTCGTATTCACATCAACCATGCCATCTACATTGAAAAAGTTGTGGTTCCTAAAGACAAACCCTACATTCTCCTCGAAGGAGACCTTGATCACTATCCTGTGATTGAATTTGGAGATGGTGGAAATGTTGTTACAAGTCCTACATTTATAGCACACGCGGATAATTTCATTGCAAAGAATATAATATTCAAGTGTAGCTTCCTTAGTGTGCAAGACACATTGAGTGATGCCCGAGGCCGTCACTATTTTTATGAATGCACCATTAAAGGTGCCATAGACTTCATCTTTGGCTCTGGCCAGTCACTTTATGAGAAATGCCAAATAGTTTCAGTGACTGACGTGATAGGGTTTCCTGGGTATATCACAGCCAATGGTCGTGGTGGTCCAGATGACCCAGCTGGGTTTGTGTTCAAAGATTGCCATGTATCTGGAACAGGCCCTATAAATCTGGGAAGGGCATACGGCAATCACTCTAGGGTTTTGTTTTCTGGAACGTACATGGAAAATATTATAACCCCACAGGGCTGGATGGCTGCATGGAACACTGGCAACGAGGATTTAATTTCATACTCGGAAGCAAACTGTAGTGGACCAGGGGCAGATTTGTCCCAACGTGTGACTTGGGAGAAGAAGCTATCGGATGAGGAAGTGGAAAGTTTAACAAATGCGGCTAAATTCATAGACCAAGATGGATGGTTAGCAAAGCAGCCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

300

Amino Acids

32.78

Weight (kDa)

4.93

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AasI GACNNNNNNGTC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 452
AccII CGCG 1 cut(s) 341
AciI CCGC 3 cut(s) 96, 341, 851
AcoI YGGCCR 2 cut(s) 118, 478
AcsI RAATTY 3 cut(s) 127, 290, 857
AfaI GTAC 1 cut(s) 680
AflIII ACRYGT 1 cut(s) 796
AgsI TTSAA 6 cut(s) 74, 168, 218, 290, 373, 595
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 2 cut(s) 535, 775
AluBI AGCT 4 cut(s) 59, 381, 581, 817
AluI AGCT 4 cut(s) 59, 381, 581, 817
Alw26I GTCTC 1 cut(s) 258
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 414
AoxI GGCC 4 cut(s) 118, 418, 478, 619
ApeKI GCWGC 2 cut(s) 716, 889
ApoI RAATTY 3 cut(s) 127, 290, 857
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 566, 620, 773
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 2 cut(s) 566, 773
BalI TGGCCA 2 cut(s) 120, 480
BanI GGYRCC 1 cut(s) 452
BbvI GCAGC 1 cut(s) 703
BccI CCATC 7 cut(s) 107, 167, 215, 293, 706, 866, 870
BceAI ACGGC 2 cut(s) 405, 661
BciT130I CCWGG 2 cut(s) 537, 777
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 1 cut(s) 657
BfmI CTRYAG 1 cut(s) 766
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 4 cut(s) 96, 717, 852, 890
BlsI GCNGC 4 cut(s) 97, 718, 853, 891
Bme1390I CCNGG 2 cut(s) 537, 777
Bme18I GGWCC 2 cut(s) 566, 773
BmeT110I CYCGRG 1 cut(s) 414
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 3 cut(s) 566, 620, 773
BmiI GGNNCC 2 cut(s) 231, 454
BmrFI CCNGG 2 cut(s) 537, 777
BmsI GCATC 2 cut(s) 26, 400
BsaBI GATNNNNATC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 4 cut(s) 121, 415, 536, 776
Bse1I ACTGG 2 cut(s) 481, 733
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 2 cut(s) 537, 777
BseDI CCNNGG 4 cut(s) 121, 415, 536, 776
BseGI GGATG 4 cut(s) 159, 717, 829, 881
BseJI GATNNNNATC 1 cut(s) 276
BseMI GCAATG 1 cut(s) 351
BseNI ACTGG 2 cut(s) 481, 733
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 1 cut(s) 703
BseYI CCCAGC 2 cut(s) 577, 581
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 4 cut(s) 120, 420, 480, 621
BshNI GGYRCC 1 cut(s) 452
BsiHKCI CYCGRG 1 cut(s) 414
BslFI GGGAC 1 cut(s) 775
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 775
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 4 cut(s) 120, 420, 480, 621
Bso31I GGTCTC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 414
Bsp143I GATC 2 cut(s) 109, 271
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 3 cut(s) 96, 341, 851
BspANI GGCC 4 cut(s) 120, 420, 480, 621
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 2 cut(s) 231, 454
BspT107I GGYRCC 1 cut(s) 452
BspTNI GGTCTC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 2 cut(s) 481, 733
BssECI CCNNGG 4 cut(s) 121, 415, 536, 776
BssMI GATC 2 cut(s) 109, 271
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 2 cut(s) 537, 777
Bst4CI ACNGT 3 cut(s) 139, 180, 767
Bst6I CTCTTC 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 61, 147
BstDEI CTNAG 1 cut(s) 386
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 4 cut(s) 159, 717, 829, 881
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 2 cut(s) 112, 274
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 2 cut(s) 109, 271
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 2 cut(s) 537, 777
BstSCI CCNGG 2 cut(s) 535, 775
BstSFI CTRYAG 1 cut(s) 766
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 703
BstXI CCANNNNNNTGG 2 cut(s) 611, 708
BsuRI GGCC 4 cut(s) 120, 420, 480, 621
BtgI CCRYGG 1 cut(s) 121
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 4 cut(s) 159, 717, 829, 881
BtsIMutI CAGTG 3 cut(s) 185, 519, 726
Cac8I GCNNGC 2 cut(s) 61, 147
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 95
Cfr13I GGNCC 3 cut(s) 566, 620, 773
Csp6I GTAC 1 cut(s) 679
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviAII CATG 5 cut(s) 122, 203, 605, 682, 720
CviQI GTAC 1 cut(s) 679
DdeI CTNAG 1 cut(s) 386
DpnI GATC 2 cut(s) 111, 273
DpnII GATC 2 cut(s) 109, 271
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
EaeI YGGCCR 2 cut(s) 118, 478
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 121
Eco31I GGTCTC 1 cut(s) 258
Eco47I GGWCC 2 cut(s) 566, 773
Eco88I CYCGRG 1 cut(s) 414
EcoO109I RGGNCCY 1 cut(s) 620
EcoRII CCWGG 2 cut(s) 535, 775
EcoT14I CCWWGG 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 5 cut(s) 125, 206, 608, 685, 723
FalI AAGNNNNNCTT 4 cut(s) 252, 284, 365, 397
FaqI GGGAC 1 cut(s) 775
FatI CATG 5 cut(s) 121, 202, 604, 681, 719
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 4 cut(s) 96, 717, 852, 890
FokI GGATG 4 cut(s) 146, 724, 836, 888
Fsp4HI GCNGC 4 cut(s) 96, 717, 852, 890
FspBI CTAG 1 cut(s) 657
GlaI GCGC 1 cut(s) 94
GluI GCNGC 4 cut(s) 96, 717, 852, 890
GsaI CCCAGC 2 cut(s) 581, 585
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 120, 420, 480, 621
HhaI GCGC 1 cut(s) 95
Hin1II CATG 5 cut(s) 125, 206, 608, 685, 723
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 2 cut(s) 155, 184
Hpy166II GTNNAC 2 cut(s) 183, 773
Hpy188I TCNGA 3 cut(s) 114, 757, 823
Hpy188III TCNNGA 4 cut(s) 74, 569, 612, 672
Hpy8I GTNNAC 2 cut(s) 183, 773
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 3 cut(s) 174, 254, 630
HpyCH4III ACNGT 3 cut(s) 139, 180, 767
HpyCH4IV ACGT 3 cut(s) 522, 677, 796
HpyCH4V TGCA 6 cut(s) 4, 50, 356, 394, 441, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 3 cut(s) 522, 677, 796
Hsp92II CATG 5 cut(s) 125, 206, 608, 685, 723
HspAI GCGC 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 2 cut(s) 109, 271
Lsp1109I GCAGC 1 cut(s) 703
LweI GCATC 2 cut(s) 26, 400
MaeI CTAG 1 cut(s) 657
MaeII ACGT 3 cut(s) 522, 677, 796
MaeIII GTNAC 5 cut(s) 313, 422, 483, 514, 799
MalI GATC 2 cut(s) 111, 273
MboI GATC 2 cut(s) 109, 271
MboII GAAGA 3 cut(s) 25, 62, 823
MlsI TGGCCA 2 cut(s) 120, 480
MluCI AATT 5 cut(s) 127, 290, 346, 744, 857
MluNI TGGCCA 2 cut(s) 120, 480
MlyI GAGTC 1 cut(s) 178
MnlI CCTC 4 cut(s) 266, 410, 730, 820
Mox20I TGGCCA 2 cut(s) 120, 480
MscI TGGCCA 2 cut(s) 120, 480
MseI TTAA 3 cut(s) 447, 743, 842
Msp20I TGGCCA 2 cut(s) 120, 480
MspA1I CMGCKG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 537, 777
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 2 cut(s) 537, 777
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 1 cut(s) 56
NcoI CCATGG 1 cut(s) 121
NdeII GATC 2 cut(s) 109, 271
NlaIII CATG 5 cut(s) 125, 206, 608, 685, 723
NlaIV GGNNCC 2 cut(s) 231, 454
NmuCI GTSAC 4 cut(s) 422, 483, 514, 799
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 443
PfeI GAWTC 1 cut(s) 155
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 4 cut(s) 97, 718, 853, 891
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PsiI TTATAA 1 cut(s) 695
Psp6I CCWGG 2 cut(s) 535, 775
PspFI CCCAGC 2 cut(s) 577, 581
PspGI CCWGG 2 cut(s) 535, 775
PspN4I GGNNCC 2 cut(s) 231, 454
PspPI GGNCC 3 cut(s) 566, 620, 773
PstNI CAGNNNCTG 1 cut(s) 518
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 1 cut(s) 680
RsaNI GTAC 1 cut(s) 679
SaqAI TTAA 3 cut(s) 447, 743, 842
SatI GCNGC 4 cut(s) 96, 717, 852, 890
Sau3AI GATC 2 cut(s) 109, 271
Sau96I GGNCC 3 cut(s) 566, 620, 773
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 2 cut(s) 537, 777
SetI ASST 9 cut(s) 61, 270, 383, 454, 525, 583, 680, 799, 819
SfaNI GCATC 2 cut(s) 26, 400
SfcI CTRYAG 1 cut(s) 766
SinI GGWCC 2 cut(s) 566, 773
Sse9I AATT 5 cut(s) 127, 290, 346, 744, 857
SsiI CCGC 3 cut(s) 96, 341, 851
SspI AATATT 2 cut(s) 369, 691
SspMI CTAG 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 535, 775
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 3 cut(s) 139, 180, 767
TaiI ACGT 3 cut(s) 525, 680, 799
TaqI TCGA 2 cut(s) 108, 258
TasI AATT 5 cut(s) 127, 290, 346, 744, 857
TauI GCSGC 2 cut(s) 98, 854
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 3 cut(s) 447, 743, 842
Tru9I TTAA 3 cut(s) 447, 743, 842
TscAI CASTG 3 cut(s) 185, 519, 733
TseFI GTSAC 4 cut(s) 422, 483, 514, 799
TseI GCWGC 2 cut(s) 716, 889
Tsp45I GTSAC 4 cut(s) 422, 483, 514, 799
TspDTI ATGAA 5 cut(s) 340, 450, 454, 738, 850
TspRI CASTG 3 cut(s) 185, 519, 733
Tth111I GACNNNGTC 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 566, 773
XapI RAATTY 3 cut(s) 127, 290, 857
XspI CTAG 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.