RLG00000017701

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001788
Physical Location & Seq
Reverse (-)
20202160 .. 20203798
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017701

Sequence Viewer

Length: 951 bp
ATGAAATTTGATCTAAATGATGAGGTATCACGTACCATTACAGTGGATATGAAGGGTCGAGGAGATTTTAGAACAGTTCAAGAAGCCATTGATTCCATACCCTCACATAACTCTTGGTGGATTCGAATCCAACTTAACAATGGTCTCTACTTTGAAAAAGTGCTGATTCCTGAAGACAAACCATACATTATTCTTGAAGGTCACCCTAAATATCTCACTGCAATAGAATTTGGAGACTTTGGCAGTGTTACAAAGAGTCCTACATTTACGTTACTTGCAGATAATTTCGTAGCTCGGAATATACTAATCAAGAATTCGTATGACCATCTTATCAAAGTGGATCCTAGTAACCGGAGAACTACTTGGGCAGTTGCGGCTAAAATTGTTGGAGACAAAGCAAGTTTTTATCATTGTGGTTTCATTAGCTTGCAAGATACAGTGTGGGATGCCAAAGGCCGTCACTATTTCTATGATTGCGATGTTCGTGGTGCTATAGACTTTATTTGGGGCAATGGCCAGTCAATTTATGAGAAATGTCAGATAATGTCTTTGAGCAAATTAATTGGCACTACTGGCTATATTACAGCACAAGGTCGTGAAGGTCCAGACGAAACTACTGGTTTTGTATTCAAAGATTGTTATGTATGTGGATCGGGCACTGCATTTCTTGGGAGAGCATACAGAGACTACTCAAGAGTCTTGTTTTCAGGGACGTATATGGAAAATGTTATTACCCCGGAGGGTTGGTCCCTTTGGACACCGCCACAAATTCAACATGCGGATGTGATCACATTCTCAGAGGCGAATTGCAGAGGACCTGGAGCAGATATGTCCCGTCGTATTTGGTGGGAGAAGAAGCTATCAGATGAGGAAGTGATAAATTTAACAAATACAGCTTCTTTCATAGACCAAGATGGCTGGTTGGGAAAGCAACCGCACGCATTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

317

Amino Acids

35.84

Weight (kDa)

5.68

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 13 - 303 8.9e-56 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 374, 761, 779, 935
AclWI GGATC 3 cut(s) 335, 348, 658
AcoI YGGCCR 1 cut(s) 514
AcsI RAATTY 5 cut(s) 5, 227, 313, 768, 880
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 1 cut(s) 34
AgsI TTSAA 5 cut(s) 80, 155, 197, 631, 773
AjnI CCWGG 1 cut(s) 817
AluBI AGCT 4 cut(s) 293, 426, 859, 896
AluI AGCT 4 cut(s) 293, 426, 859, 896
Alw26I GTCTC 4 cut(s) 149, 228, 384, 678
AlwI GGATC 3 cut(s) 335, 348, 658
AoxI GGCC 2 cut(s) 454, 514
ApoI RAATTY 5 cut(s) 5, 227, 313, 768, 880
AseI ATTAAT 1 cut(s) 560
AspS9I GGNCC 3 cut(s) 602, 747, 815
AsuC2I CCSGG 1 cut(s) 737
AsuHPI GGTGA 1 cut(s) 194
AsuII TTCGAA 1 cut(s) 124
AvaII GGWCC 3 cut(s) 602, 747, 815
BaeGI GKGCMC 1 cut(s) 659
BalI TGGCCA 1 cut(s) 516
BamHI GGATCC 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 180
BccI CCATC 2 cut(s) 333, 908
BceAI ACGGC 1 cut(s) 441
BciT130I CCWGG 1 cut(s) 819
BclI TGATCA 1 cut(s) 786
BcnI CCSGG 1 cut(s) 737
BcoDI GTCTC 4 cut(s) 149, 228, 384, 678
BfaI CTAG 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 492
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 737, 819
Bme18I GGWCC 3 cut(s) 602, 747, 815
BmgT120I GGNCC 3 cut(s) 602, 747, 815
BmiI GGNNCC 2 cut(s) 342, 749
BmrFI CCNGG 2 cut(s) 737, 819
BmsI GCATC 1 cut(s) 436
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 840
Bpu14I TTCGAA 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 676
BpuMI CCSGG 1 cut(s) 737
BsaAI YACGTR 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 735
BsaWI WCCGGW 1 cut(s) 351
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bse1I ACTGG 3 cut(s) 517, 577, 622
Bse3DI GCAATG 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 1 cut(s) 819
BseDI CCNNGG 1 cut(s) 735
BseGI GGATG 2 cut(s) 451, 787
BseJI GATNNNNATC 1 cut(s) 125
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 1 cut(s) 810
BseNI ACTGG 3 cut(s) 517, 577, 622
BseRI GAGGAG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 659
BshFI GGCC 2 cut(s) 456, 516
BsiSI CCGG 2 cut(s) 352, 737
BslFI GGGAC 3 cut(s) 724, 733, 817
BsmAI GTCTC 4 cut(s) 149, 228, 384, 678
BsmFI GGGAC 3 cut(s) 724, 733, 817
BsnI GGCC 2 cut(s) 456, 516
Bso31I GGTCTC 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 4 cut(s) 10, 340, 650, 786
BspACI CCGC 4 cut(s) 374, 761, 779, 935
BspANI GGCC 2 cut(s) 456, 516
BspCNI CTCAG 1 cut(s) 809
BspLI GGNNCC 2 cut(s) 342, 749
BspPI GGATC 3 cut(s) 335, 348, 658
BspT104I TTCGAA 1 cut(s) 124
BspTNI GGTCTC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 517
BsrI ACTGG 3 cut(s) 517, 577, 622
BssECI CCNNGG 1 cut(s) 735
BssMI GATC 4 cut(s) 10, 340, 650, 786
Bst2UI CCWGG 1 cut(s) 819
Bst4CI ACNGT 3 cut(s) 43, 76, 439
BstBAI YACGTR 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 428, 939
BstDEI CTNAG 1 cut(s) 796
BstEII GGTNACC 1 cut(s) 200
BstF5I GGATG 2 cut(s) 451, 787
BstKTI GATC 4 cut(s) 13, 343, 653, 789
BstMAI GTCTC 4 cut(s) 149, 228, 384, 678
BstMBI GATC 4 cut(s) 10, 340, 650, 786
BstMWI GCNNNNNNNGC 2 cut(s) 374, 573
BstNI CCWGG 1 cut(s) 819
BstNSI RCATGY 1 cut(s) 779
BstPI GGTNACC 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 735, 817
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 1 cut(s) 659
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 340
BsuRI GGCC 2 cut(s) 456, 516
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 2 cut(s) 451, 787
BtsI GCAGTG 3 cut(s) 216, 250, 657
BtsIMutI CAGTG 5 cut(s) 48, 216, 250, 444, 657
Cac8I GCNNGC 2 cut(s) 428, 939
Cfr13I GGNCC 3 cut(s) 602, 747, 815
Csp6I GTAC 1 cut(s) 33
CviAII CATG 1 cut(s) 776
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 1 cut(s) 796
DpnI GATC 4 cut(s) 12, 342, 652, 788
DpnII GATC 4 cut(s) 10, 340, 650, 786
EaeI YGGCCR 1 cut(s) 514
Eco31I GGTCTC 1 cut(s) 149
Eco47I GGWCC 3 cut(s) 602, 747, 815
Eco57I CTGAAG 1 cut(s) 192
Eco91I GGTNACC 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 815
EcoO65I GGTNACC 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 817
FaeI CATG 1 cut(s) 779
FaqI GGGAC 3 cut(s) 724, 733, 817
FatI CATG 1 cut(s) 775
FbaI TGATCA 1 cut(s) 786
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 2 cut(s) 458, 794
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 1 cut(s) 345
GluI GCNGC 1 cut(s) 375
GsuI CTGGAG 1 cut(s) 840
HaeIII GGCC 2 cut(s) 456, 516
HapII CCGG 2 cut(s) 352, 737
Hin1II CATG 1 cut(s) 779
HinfI GANTC 6 cut(s) 92, 121, 126, 166, 256, 696
HpaII CCGG 2 cut(s) 352, 737
HphI GGTGA 1 cut(s) 194
Hpy188I TCNGA 4 cut(s) 297, 540, 799, 865
Hpy188III TCNNGA 7 cut(s) 80, 170, 194, 310, 596, 605, 693
Hpy99I CGWCG 1 cut(s) 840
HpyAV CCTTC 3 cut(s) 46, 191, 593
HpyCH4III ACNGT 3 cut(s) 43, 76, 439
HpyCH4IV ACGT 3 cut(s) 31, 269, 713
HpyCH4V TGCA 5 cut(s) 221, 278, 430, 662, 810
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 573
HpyF3I CTNAG 1 cut(s) 796
HpySE526I ACGT 3 cut(s) 31, 269, 713
Hsp92II CATG 1 cut(s) 779
Ksp22I TGATCA 1 cut(s) 786
Kzo9I GATC 4 cut(s) 10, 340, 650, 786
LmnI GCTCC 1 cut(s) 821
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 345
MaeII ACGT 3 cut(s) 31, 269, 713
MaeIII GTNAC 5 cut(s) 200, 247, 270, 347, 458
MalI GATC 4 cut(s) 12, 342, 652, 788
MboI GATC 4 cut(s) 10, 340, 650, 786
MboII GAAGA 2 cut(s) 185, 865
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 659
MlsI TGGCCA 1 cut(s) 516
MluNI TGGCCA 1 cut(s) 516
MlyI GAGTC 2 cut(s) 265, 705
MmeI TCCRAC 2 cut(s) 154, 367
MnlI CCTC 7 cut(s) 16, 53, 112, 733, 793, 806, 862
Mox20I TGGCCA 1 cut(s) 516
MscI TGGCCA 1 cut(s) 516
MseI TTAA 4 cut(s) 135, 560, 884, 944
MslI CAYNNNNRTG 2 cut(s) 41, 780
Msp20I TGGCCA 1 cut(s) 516
MspI CCGG 2 cut(s) 352, 737
MspR9I CCNGG 2 cut(s) 737, 819
MvaI CCWGG 1 cut(s) 819
MwoI GCNNNNNNNGC 2 cut(s) 374, 573
NciI CCSGG 1 cut(s) 737
NdeII GATC 4 cut(s) 10, 340, 650, 786
NlaIII CATG 1 cut(s) 779
NlaIV GGNNCC 2 cut(s) 342, 749
NmuCI GTSAC 2 cut(s) 200, 458
NspI RCATGY 1 cut(s) 779
NspV TTCGAA 1 cut(s) 124
PfeI GAWTC 4 cut(s) 92, 121, 126, 166
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 2 cut(s) 264, 704
PpsI GAGTC 2 cut(s) 264, 704
Ppu21I YACGTR 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 815
PshBI ATTAAT 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 817
PspEI GGTNACC 1 cut(s) 200
PspGI CCWGG 1 cut(s) 817
PspN4I GGNNCC 2 cut(s) 342, 749
PspPI GGNCC 3 cut(s) 602, 747, 815
PspPPI RGGWCCY 1 cut(s) 815
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
RseI CAYNNNNRTG 2 cut(s) 41, 780
SaqAI TTAA 4 cut(s) 135, 560, 884, 944
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 4 cut(s) 10, 340, 650, 786
Sau96I GGNCC 3 cut(s) 602, 747, 815
SchI GAGTC 2 cut(s) 265, 705
ScrFI CCNGG 2 cut(s) 737, 819
SduI GDGCHC 1 cut(s) 659
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 492
SfuI TTCGAA 1 cut(s) 124
SinI GGWCC 3 cut(s) 602, 747, 815
SmiMI CAYNNNNRTG 2 cut(s) 41, 780
SmlI CTYRAG 1 cut(s) 691
SmoI CTYRAG 1 cut(s) 691
SsiI CCGC 4 cut(s) 374, 761, 779, 935
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 2 cut(s) 735, 817
TaaI ACNGT 3 cut(s) 43, 76, 439
TaiI ACGT 3 cut(s) 34, 272, 716
TaqI TCGA 2 cut(s) 58, 124
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 4 cut(s) 92, 121, 126, 166
Tru1I TTAA 4 cut(s) 135, 560, 884, 944
Tru9I TTAA 4 cut(s) 135, 560, 884, 944
TscAI CASTG 5 cut(s) 48, 223, 250, 444, 664
TseFI GTSAC 2 cut(s) 200, 458
Tsp45I GTSAC 2 cut(s) 200, 458
TspDTI ATGAA 4 cut(s) 17, 65, 409, 892
TspRI CASTG 5 cut(s) 48, 223, 250, 444, 664
VpaK11BI GGWCC 3 cut(s) 602, 747, 815
VspI ATTAAT 1 cut(s) 560
XapI RAATTY 5 cut(s) 5, 227, 313, 768, 880
XceI RCATGY 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.