Rh2CG210700

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001788
Physical Location & Seq
Reverse (-)
20649303 .. 20650966
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG210700.1

Sequence Viewer

Length: 990 bp
ATGAAGTTTCTCGTAATATTGGTTCTCTTCTTTTTCTTTGCTAAATTTGATGCAATTAGATATGATCTACAAGGTGATGTGTCGAGAACAATGACTGTCAGTAAGAATGGTCGAGGGCAATTTAGTACTGTACAACAAGCCATTGATTCCATACCATCAAATAATGCATGGTGGATCCGGATCCTTATTAACCATGGTGTCTACTATGAAAAGGTTACAATCCCTCAAGACAAACCGTACATTATTCTTGAAGGGAACCCTAATCATCTTACAACAATCGAATTTGGAGATGCTGGCAGTGTTGTTGACAGTCCTACATTCAAGGCACATGCAGATAATTTTGTGGCAAAAAATATAGTATTCAAGAATACTTACGACCATCTTATCACATTGGAGCCGAATGGAAGGAAAACTACTTGGGCACCTGCTACTGCAATAGATGGAGACAAAGCAAGTTTCTATAACTGCACTTTCATTAGCTTGCAAGATACACTGTCTGATGCCCAAGGTCGACACTATTTCTATGACTGCAACATTCAAGGTGCTATAGACTTTATTTGGGGAAATGGCCAGTCAATTTACGAGAAATGTCAGATAACTTCGTTAGTCCACAAACTAGGCCAGAATAGTGGTTATATCACTGCACAGGGTCGCGAAAGACCTGAGGAAACTACTGGGTTTGTATTCAAAGATTGCCATGTACGTGGATCAGGCCCTATAATGCTAGGAAGAGCATACAGAGCATACGCAAGAGTATTGTTTTCTGGGACATATATGGAAAACATTGTTACACCCCAGGGCTGGTCAGAATGGCAAGTCAAACCAGTGGATTCAATCACATTCTCAGAGGCGAAATGCAGTGGACCTGGAGCGGACATGTCTCATCGGATTGCATGGGAGAAGAAGTTATCGGATGAAGAAGTCGAACAGTTAACAAATACAGCTTCTTTTATAAATCAAGATGGATGGTTAGAAAAGCAACCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

329

Amino Acids

37.16

Weight (kDa)

5.99

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 31 - 319 1.1e-58 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
AarI CACCTGC 1 cut(s) 433
Acc36I ACCTGC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 421
AccBSI CCGCTC 1 cut(s) 872
AccI GTMKAC 2 cut(s) 201, 511
AccII CGCG 1 cut(s) 654
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 1 cut(s) 872
AclWI GGATC 5 cut(s) 169, 175, 182, 188, 715
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 2 cut(s) 44, 281
AfaI GTAC 4 cut(s) 127, 132, 239, 702
AfiI CCNNNNNNNGG 1 cut(s) 801
AflIII ACRYGT 1 cut(s) 876
AgsI TTSAA 6 cut(s) 251, 322, 364, 539, 688, 834
AjnI CCWGG 2 cut(s) 795, 865
AluBI AGCT 2 cut(s) 480, 944
AluI AGCT 2 cut(s) 480, 944
Alw26I GTCTC 2 cut(s) 438, 885
AlwI GGATC 5 cut(s) 169, 175, 182, 188, 715
Aor13HI TCCGGA 1 cut(s) 177
AoxI GGCC 3 cut(s) 568, 619, 712
ApoI RAATTY 2 cut(s) 44, 281
AspS9I GGNCC 2 cut(s) 713, 863
AsuHPI GGTGA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 863
AxyI CCTNAGG 1 cut(s) 663
BaeGI GKGCMC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 570
BamHI GGATCC 2 cut(s) 174, 180
BanI GGYRCC 1 cut(s) 421
BarI GAAGNNNNNNTAC 2 cut(s) 397, 429
BccI CCATC 5 cut(s) 163, 387, 434, 956, 960
BciT130I CCWGG 2 cut(s) 797, 867
BcoDI GTCTC 2 cut(s) 438, 885
BfaI CTAG 2 cut(s) 617, 725
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 433
BmcAI AGTACT 1 cut(s) 127
Bme1390I CCNGG 2 cut(s) 797, 867
Bme18I GGWCC 1 cut(s) 863
BmgT120I GGNCC 2 cut(s) 713, 863
BmiI GGNNCC 5 cut(s) 176, 182, 257, 396, 423
BmrFI CCNGG 2 cut(s) 797, 867
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 3 cut(s) 40, 280, 490
BmuI ACTGGG 1 cut(s) 684
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 704
BsaBI GATNNNNATC 1 cut(s) 179
BsaJI CCNNGG 4 cut(s) 193, 505, 795, 796
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 890, 920
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse1I ACTGG 3 cut(s) 571, 679, 824
Bse21I CCTNAGG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 179
BseAI TCCGGA 1 cut(s) 177
BseBI CCWGG 2 cut(s) 797, 867
BseDI CCNNGG 4 cut(s) 193, 505, 795, 796
BseGI GGATG 2 cut(s) 919, 971
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 801
BseMII CTCAG 2 cut(s) 654, 858
BseNI ACTGG 3 cut(s) 571, 679, 824
BseSI GKGCMC 1 cut(s) 424
BsgI GTGCAG 2 cut(s) 451, 627
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 3 cut(s) 570, 621, 714
BshNI GGYRCC 1 cut(s) 421
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 781
BslI CCNNNNNNNGG 1 cut(s) 801
BsmAI GTCTC 2 cut(s) 438, 885
BsmFI GGGAC 1 cut(s) 781
BsnI GGCC 3 cut(s) 570, 621, 714
Bsp1286I GDGCHC 1 cut(s) 424
Bsp13I TCCGGA 1 cut(s) 177
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 4 cut(s) 64, 174, 180, 707
Bsp19I CCATGG 1 cut(s) 193
Bsp68I TCGCGA 1 cut(s) 654
BspACI CCGC 1 cut(s) 872
BspANI GGCC 3 cut(s) 570, 621, 714
BspCNI CTCAG 2 cut(s) 655, 857
BspEI TCCGGA 1 cut(s) 177
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 5 cut(s) 176, 182, 257, 396, 423
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 5 cut(s) 169, 175, 182, 188, 715
BspQI GCTCTTC 1 cut(s) 724
BspT107I GGYRCC 1 cut(s) 421
BsrBI CCGCTC 1 cut(s) 872
BsrGI TGTACA 1 cut(s) 130
BsrI ACTGG 3 cut(s) 571, 679, 824
BssECI CCNNGG 4 cut(s) 193, 505, 795, 796
BssMI GATC 4 cut(s) 64, 174, 180, 707
BssT1I CCWWGG 2 cut(s) 193, 505
Bst2UI CCWGG 2 cut(s) 797, 867
Bst4CI ACNGT 6 cut(s) 97, 130, 237, 311, 495, 930
Bst6I CTCTTC 2 cut(s) 32, 724
BstAUI TGTACA 1 cut(s) 130
BstBAI YACGTR 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 295, 482
BstDEI CTNAG 2 cut(s) 663, 844
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 2 cut(s) 919, 971
BstFNI CGCG 1 cut(s) 654
BstKTI GATC 4 cut(s) 67, 177, 183, 710
BstMAI GTCTC 2 cut(s) 438, 885
BstMBI GATC 4 cut(s) 64, 174, 180, 707
BstMWI GCNNNNNNNGC 1 cut(s) 740
BstNI CCWGG 2 cut(s) 797, 867
BstNSI RCATGY 2 cut(s) 332, 880
BstSCI CCNGG 2 cut(s) 795, 865
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 424
BstUI CGCG 1 cut(s) 654
BstX2I RGATCY 2 cut(s) 174, 180
BstXI CCANNNNNNTGG 2 cut(s) 629, 704
BstYI RGATCY 2 cut(s) 174, 180
Bsu36I CCTNAGG 1 cut(s) 663
BsuRI GGCC 3 cut(s) 570, 621, 714
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 919, 971
BtsI GCAGTG 3 cut(s) 304, 639, 865
BtsIMutI CAGTG 5 cut(s) 304, 491, 639, 831, 865
BtuMI TCGCGA 1 cut(s) 654
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 2 cut(s) 295, 482
Cfr13I GGNCC 2 cut(s) 713, 863
Csp6I GTAC 4 cut(s) 126, 131, 238, 701
CviAII CATG 6 cut(s) 168, 194, 329, 698, 877, 894
CviJI RGCY 8 cut(s) 140, 397, 480, 570, 621, 714, 801, 944
CviKI_1 RGCY 8 cut(s) 140, 397, 480, 570, 621, 714, 801, 944
CviQI GTAC 4 cut(s) 126, 131, 238, 701
DdeI CTNAG 2 cut(s) 663, 844
DpnI GATC 4 cut(s) 66, 176, 182, 709
DpnII GATC 4 cut(s) 64, 174, 180, 707
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 2 cut(s) 32, 724
EarI CTCTTC 2 cut(s) 32, 724
Eco130I CCWWGG 2 cut(s) 193, 505
Eco47I GGWCC 1 cut(s) 863
Eco81I CCTNAGG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 795, 865
EcoT14I CCWWGG 2 cut(s) 193, 505
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 2 cut(s) 193, 505
FaeI CATG 6 cut(s) 171, 197, 332, 701, 880, 897
FaqI GGGAC 1 cut(s) 781
FatI CATG 6 cut(s) 167, 193, 328, 697, 876, 893
FblI GTMKAC 2 cut(s) 201, 511
FokI GGATG 2 cut(s) 926, 978
FspBI CTAG 2 cut(s) 617, 725
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 3 cut(s) 570, 621, 714
HapII CCGG 1 cut(s) 178
Hin1II CATG 6 cut(s) 171, 197, 332, 701, 880, 897
HincII GTYRAC 3 cut(s) 307, 512, 933
HindII GTYRAC 3 cut(s) 307, 512, 933
HinfI GANTC 2 cut(s) 146, 830
HpaI GTTAAC 1 cut(s) 933
HpaII CCGG 1 cut(s) 178
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 6 cut(s) 202, 307, 512, 610, 863, 933
Hpy188I TCNGA 6 cut(s) 499, 594, 808, 847, 888, 913
Hpy188III TCNNGA 7 cut(s) 84, 178, 227, 248, 364, 653, 959
Hpy8I GTNNAC 6 cut(s) 202, 307, 512, 610, 863, 933
HpyAV CCTTC 2 cut(s) 245, 399
HpyCH4III ACNGT 6 cut(s) 97, 130, 237, 311, 495, 930
HpyCH4IV ACGT 1 cut(s) 703
HpyF10VI GCNNNNNNNGC 1 cut(s) 740
HpyF3I CTNAG 2 cut(s) 663, 844
HpySE526I ACGT 1 cut(s) 703
Hsp92II CATG 6 cut(s) 171, 197, 332, 701, 880, 897
Kpn2I TCCGGA 1 cut(s) 177
KspAI GTTAAC 1 cut(s) 933
Kzo9I GATC 4 cut(s) 64, 174, 180, 707
LguI GCTCTTC 1 cut(s) 724
LmnI GCTCC 2 cut(s) 394, 869
LweI GCATC 3 cut(s) 40, 280, 490
MaeI CTAG 2 cut(s) 617, 725
MaeII ACGT 1 cut(s) 703
MaeIII GTNAC 2 cut(s) 214, 787
MalI GATC 4 cut(s) 66, 176, 182, 709
MbiI CCGCTC 1 cut(s) 872
MboI GATC 4 cut(s) 64, 174, 180, 707
MboII GAAGA 4 cut(s) 19, 741, 913, 929
MflI RGATCY 2 cut(s) 174, 180
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 6 cut(s) 44, 54, 119, 281, 337, 576
MluNI TGGCCA 1 cut(s) 570
MnlI CCTC 4 cut(s) 107, 234, 658, 841
Mox20I TGGCCA 1 cut(s) 570
Mph1103I ATGCAT 1 cut(s) 169
MroI TCCGGA 1 cut(s) 177
MscI TGGCCA 1 cut(s) 570
MseI TTAA 3 cut(s) 189, 932, 988
MslI CAYNNNNRTG 1 cut(s) 702
Msp20I TGGCCA 1 cut(s) 570
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 2 cut(s) 797, 867
MvaI CCWGG 2 cut(s) 797, 867
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 1 cut(s) 740
NcoI CCATGG 1 cut(s) 193
NdeII GATC 4 cut(s) 64, 174, 180, 707
NlaIII CATG 6 cut(s) 171, 197, 332, 701, 880, 897
NlaIV GGNNCC 5 cut(s) 176, 182, 257, 396, 423
NruI TCGCGA 1 cut(s) 654
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 332, 880
PaqCI CACCTGC 1 cut(s) 433
PasI CCCWGGG 1 cut(s) 796
PciI ACATGT 1 cut(s) 876
PciSI GCTCTTC 1 cut(s) 724
PfeI GAWTC 2 cut(s) 146, 830
Ppu21I YACGTR 1 cut(s) 704
PscI ACATGT 1 cut(s) 876
PsiI TTATAA 1 cut(s) 953
Psp6I CCWGG 2 cut(s) 795, 865
PspGI CCWGG 2 cut(s) 795, 865
PspN4I GGNNCC 5 cut(s) 176, 182, 257, 396, 423
PspPI GGNCC 2 cut(s) 713, 863
PsrI GAACNNNNNNTAC 1 cut(s) 38
PsuI RGATCY 2 cut(s) 174, 180
RruI TCGCGA 1 cut(s) 654
RsaI GTAC 4 cut(s) 127, 132, 239, 702
RsaNI GTAC 4 cut(s) 126, 131, 238, 701
RseI CAYNNNNRTG 1 cut(s) 702
SalI GTCGAC 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 724
SaqAI TTAA 3 cut(s) 189, 932, 988
Sau3AI GATC 4 cut(s) 64, 174, 180, 707
Sau96I GGNCC 2 cut(s) 713, 863
ScaI AGTACT 1 cut(s) 127
ScrFI CCNGG 2 cut(s) 797, 867
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 3 cut(s) 40, 280, 490
SfcI CTRYAG 1 cut(s) 546
SinI GGWCC 1 cut(s) 863
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
Sse9I AATT 6 cut(s) 44, 54, 119, 281, 337, 576
SsiI CCGC 1 cut(s) 872
SspI AATATT 1 cut(s) 18
SspMI CTAG 2 cut(s) 617, 725
StyD4I CCNGG 2 cut(s) 795, 865
StyI CCWWGG 2 cut(s) 193, 505
TaaI ACNGT 6 cut(s) 97, 130, 237, 311, 495, 930
TaiI ACGT 1 cut(s) 706
TaqI TCGA 5 cut(s) 83, 112, 279, 511, 924
TasI AATT 6 cut(s) 44, 54, 119, 281, 337, 576
TatI WGTACW 2 cut(s) 125, 130
TfiI GAWTC 2 cut(s) 146, 830
Tru1I TTAA 3 cut(s) 189, 932, 988
Tru9I TTAA 3 cut(s) 189, 932, 988
TscAI CASTG 5 cut(s) 304, 498, 646, 831, 865
TspDTI ATGAA 4 cut(s) 17, 222, 463, 930
TspRI CASTG 5 cut(s) 304, 498, 646, 831, 865
VpaK11BI GGWCC 1 cut(s) 863
XapI RAATTY 2 cut(s) 44, 281
XceI RCATGY 2 cut(s) 332, 880
XmiI GTMKAC 2 cut(s) 201, 511
XspI CTAG 2 cut(s) 617, 725
ZrmI AGTACT 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.