Rroxscaffold_6G00422080

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001788
Physical Location & Seq
Forward (+)
43086776 .. 43089616
2841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00422080.1

Sequence Viewer

Length: 708 bp
ATGGAGTTCTTGGCATCTTGGAGCTCATTTGGTAAAAGACTAAATTGTGGTGACCGTGATATTGGCTCTTTTCTCTCTTTTCTGAATGGATTGGATGCATTCCGTCCACTAGACCAAGTTTCAACTACCATCGTAGTTGATCAGAATGGTAAAGGACAATTTACAACTGTTCAACAAGCTGTCGACTCTATACCACCAAATAACCCGGCGTGGACTCTAATTCATCTCAACTCCGGTAATACCTATGATAATCTTCCTTCTAAATTAGACGCCAACGGGGTCAACACTACTTGGGCACCTGCAGCTGCAATTGCCGGAGACAGAGCAAGTTTTTACCATTGCAGCTTCATTAGCTTGCAGGACACATTGACTGATGCCAAAGGTCGTCATTACTTCTATGACTGCCAAATTTCCGGCGCCATAGACTTCATTTGGGGGAATGCACAATCAATTTACGAGAATTGCAAGATCAATTCATTGGCATCAAAAATAGCTCCACGGGTTGGTTTTATTACAGCACAGGCTCGGGAAAGTCCAGAGGAAAGTACTGGTTTTGTATTCAAAAATTGTCATGTGACTGGATCTGGCCCTATATATCTAGGAAGAGCTCATACAAACTACGCAAGAGTATTGTTTGCTGGGTCAACAATGGACAATGTTATTACGCCAGAAGGTTGGAACGGGGCACGGTTTAAGAGCTCCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

235

Amino Acids

25.65

Weight (kDa)

5.94

Isoelectric Point (pI)

25.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 93 - 227 6.3e-31 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 307
Acc36I ACCTGC 1 cut(s) 307
AccB1I GGYRCC 2 cut(s) 295, 416
AccB7I CCANNNNNTGG 1 cut(s) 503
AccI GTMKAC 1 cut(s) 183
AclWI GGATC 1 cut(s) 589
AcsI RAATTY 1 cut(s) 408
AcyI GRCGYC 2 cut(s) 270, 417
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 1 cut(s) 503
AgsI TTSAA 3 cut(s) 123, 173, 562
AluBI AGCT 8 cut(s) 24, 179, 305, 345, 354, 494, 608, 699
AluI AGCT 8 cut(s) 24, 179, 305, 345, 354, 494, 608, 699
Alw21I GWGCWC 3 cut(s) 26, 610, 701
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 1 cut(s) 589
Ama87I CYCGRG 1 cut(s) 525
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 3 cut(s) 302, 305, 342
ApoI RAATTY 1 cut(s) 408
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 587
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 62
AvaI CYCGRG 1 cut(s) 525
BaeGI GKGCMC 2 cut(s) 298, 688
BanI GGYRCC 2 cut(s) 295, 416
BanII GRGCYC 3 cut(s) 26, 610, 701
BarI GAAGNNNNNNTAC 2 cut(s) 595, 627
Bbv12I GWGCWC 3 cut(s) 26, 610, 701
BbvI GCAGC 3 cut(s) 292, 314, 354
BccI CCATC 1 cut(s) 137
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 2 cut(s) 110, 599
BfmI CTRYAG 1 cut(s) 300
BfoI RGCGCY 1 cut(s) 420
BfuAI ACCTGC 1 cut(s) 307
BisI GCNGC 3 cut(s) 303, 306, 343
BlsI GCNGC 3 cut(s) 304, 307, 344
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 525
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 2 cut(s) 297, 418
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 4 cut(s) 23, 85, 364, 491
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 2 cut(s) 270, 417
BsaJI CCNNGG 1 cut(s) 497
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 553, 583
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 497
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMI GCAATG 1 cut(s) 337
BseNI ACTGG 2 cut(s) 553, 583
BseSI GKGCMC 2 cut(s) 298, 688
BseXI GCAGC 3 cut(s) 292, 314, 354
BseYI CCCAGC 1 cut(s) 638
BshFI GGCC 1 cut(s) 588
BshNI GGYRCC 2 cut(s) 295, 416
BsiHKAI GWGCWC 3 cut(s) 26, 610, 701
BsiHKCI CYCGRG 1 cut(s) 525
BsiSI CCGG 4 cut(s) 206, 234, 315, 414
BslI CCNNNNNNNGG 1 cut(s) 503
BsmAI GTCTC 1 cut(s) 312
BsmI GAATGC 2 cut(s) 98, 445
BsnI GGCC 1 cut(s) 588
BsoBI CYCGRG 1 cut(s) 525
Bsp1286I GDGCHC 5 cut(s) 26, 298, 610, 688, 701
Bsp143I GATC 3 cut(s) 139, 468, 581
BspANI GGCC 1 cut(s) 588
BspLI GGNNCC 2 cut(s) 297, 418
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 307
BspPI GGATC 1 cut(s) 589
BspQI GCTCTTC 1 cut(s) 598
BspT107I GGYRCC 2 cut(s) 295, 416
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 2 cut(s) 553, 583
BssECI CCNNGG 1 cut(s) 497
BssMI GATC 3 cut(s) 139, 468, 581
BssNI GRCGYC 2 cut(s) 270, 417
Bst4CI ACNGT 3 cut(s) 56, 169, 690
Bst6I CTCTTC 1 cut(s) 598
BstACI GRCGYC 2 cut(s) 270, 417
BstC8I GCNNGC 1 cut(s) 356
BstDSI CCRYGG 1 cut(s) 497
BstEII GGTNACC 1 cut(s) 50
BstF5I GGATG 1 cut(s) 100
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 3 cut(s) 142, 471, 584
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 3 cut(s) 139, 468, 581
BstMWI GCNNNNNNNGC 3 cut(s) 302, 311, 351
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 2 cut(s) 298, 688
BstV1I GCAGC 3 cut(s) 292, 314, 354
BstX2I RGATCY 1 cut(s) 581
BstXI CCANNNNNNTGG 1 cut(s) 675
BstYI RGATCY 1 cut(s) 581
BsuRI GGCC 1 cut(s) 588
BtgI CCRYGG 1 cut(s) 497
BtsCI GGATG 1 cut(s) 100
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 356
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 546
CviAII CATG 1 cut(s) 572
CviQI GTAC 1 cut(s) 546
DinI GGCGCC 1 cut(s) 418
DpnI GATC 3 cut(s) 141, 470, 583
DpnII GATC 3 cut(s) 139, 468, 581
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Ecl136II GAGCTC 3 cut(s) 24, 608, 699
Eco24I GRGCYC 3 cut(s) 26, 610, 701
Eco53kI GAGCTC 3 cut(s) 24, 608, 699
Eco88I CYCGRG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 50
EcoICRI GAGCTC 3 cut(s) 24, 608, 699
EcoO65I GGTNACC 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 100
EcoT38I GRGCYC 3 cut(s) 26, 610, 701
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
FaeI CATG 1 cut(s) 575
FaiI YATR 8 cut(s) 191, 246, 399, 422, 573, 593, 595, 612
FatI CATG 1 cut(s) 571
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 3 cut(s) 303, 306, 343
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 3 cut(s) 26, 610, 701
Fsp4HI GCNGC 3 cut(s) 303, 306, 343
FspBI CTAG 2 cut(s) 110, 599
GlaI GCGC 1 cut(s) 418
GluI GCNGC 3 cut(s) 303, 306, 343
GsaI CCCAGC 1 cut(s) 642
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 1 cut(s) 588
HapII CCGG 4 cut(s) 206, 234, 315, 414
HgaI GACGC 1 cut(s) 278
HhaI GCGC 1 cut(s) 419
Hin1I GRCGYC 2 cut(s) 270, 417
Hin1II CATG 1 cut(s) 575
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HincII GTYRAC 3 cut(s) 184, 283, 645
HindII GTYRAC 3 cut(s) 184, 283, 645
HinfI GANTC 2 cut(s) 185, 214
HpaII CCGG 4 cut(s) 206, 234, 315, 414
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 5 cut(s) 107, 184, 213, 283, 645
Hpy188I TCNGA 3 cut(s) 84, 144, 703
Hpy188III TCNNGA 2 cut(s) 527, 536
Hpy8I GTNNAC 5 cut(s) 107, 184, 213, 283, 645
HpyAV CCTTC 2 cut(s) 267, 665
HpyCH4III ACNGT 3 cut(s) 56, 169, 690
HpyCH4V TGCA 7 cut(s) 98, 302, 308, 342, 358, 443, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 311, 351
Hsp92I GRCGYC 2 cut(s) 270, 417
Hsp92II CATG 1 cut(s) 575
HspAI GCGC 1 cut(s) 417
KasI GGCGCC 1 cut(s) 416
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 3 cut(s) 139, 468, 581
LguI GCTCTTC 1 cut(s) 598
LmnI GCTCC 3 cut(s) 21, 499, 704
Lsp1109I GCAGC 3 cut(s) 292, 314, 354
LweI GCATC 4 cut(s) 23, 85, 364, 491
MaeI CTAG 2 cut(s) 110, 599
MaeIII GTNAC 2 cut(s) 50, 574
MalI GATC 3 cut(s) 141, 470, 583
MboI GATC 3 cut(s) 139, 468, 581
MboII GAAGA 2 cut(s) 245, 615
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 5 cut(s) 26, 298, 610, 688, 701
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 2 cut(s) 179, 208
MmeI TCCRAC 1 cut(s) 656
MnlI CCTC 1 cut(s) 532
Mph1103I ATGCAT 1 cut(s) 100
MseI TTAA 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 4 cut(s) 206, 234, 315, 414
MspR9I CCNGG 1 cut(s) 206
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 98, 445
MwoI GCNNNNNNNGC 3 cut(s) 302, 311, 351
NarI GGCGCC 1 cut(s) 417
NciI CCSGG 1 cut(s) 206
NdeII GATC 3 cut(s) 139, 468, 581
NlaIII CATG 1 cut(s) 575
NlaIV GGNNCC 2 cut(s) 297, 418
NmuCI GTSAC 2 cut(s) 50, 574
NsiI ATGCAT 1 cut(s) 100
PaqCI CACCTGC 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 598
PctI GAATGC 2 cut(s) 98, 445
PflMI CCANNNNNTGG 1 cut(s) 503
PkrI GCNGC 3 cut(s) 304, 307, 344
PleI GAGTC 2 cut(s) 179, 208
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 2 cut(s) 179, 208
Psp124BI GAGCTC 3 cut(s) 26, 610, 701
PspEI GGTNACC 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 638
PspN4I GGNNCC 2 cut(s) 297, 418
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 304
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SacI GAGCTC 3 cut(s) 26, 610, 701
SalI GTCGAC 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 598
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 3 cut(s) 303, 306, 343
Sau3AI GATC 3 cut(s) 139, 468, 581
Sau96I GGNCC 1 cut(s) 587
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 2 cut(s) 179, 208
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 5 cut(s) 26, 298, 610, 688, 701
SfaNI GCATC 4 cut(s) 23, 85, 364, 491
SfcI CTRYAG 1 cut(s) 300
SfoI GGCGCC 1 cut(s) 418
SspDI GGCGCC 1 cut(s) 416
SspMI CTAG 2 cut(s) 110, 599
SstI GAGCTC 3 cut(s) 26, 610, 701
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 3 cut(s) 56, 169, 690
TaqI TCGA 1 cut(s) 183
TatI WGTACW 1 cut(s) 545
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TseFI GTSAC 2 cut(s) 50, 574
TseI GCWGC 3 cut(s) 302, 305, 342
Tsp45I GTSAC 2 cut(s) 50, 574
TspDTI ATGAA 4 cut(s) 212, 337, 418, 465
TspGWI ACGGA 1 cut(s) 92
Van91I CCANNNNNTGG 1 cut(s) 503
XapI RAATTY 1 cut(s) 408
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 2 cut(s) 110, 599
ZrmI AGTACT 1 cut(s) 547
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.