RchiOBHm_Chr3g0457241

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001788
Physical Location & Seq
Reverse (-)
6449964 .. 6451753
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42399

Sequence Viewer

Length: 1041 bp
ATGAAGTCTCTTATTCTTATTATTTCAGTGATATTGGCTCTTTTCTCTTTTCTGAATGGATTGAATGCATTCCGTCCACTAGACCAAGTTTCAACTACCATCGTAGTTGATCAGAATGGTAAAGGACAATTTACCACTGTTCAACAAGCCGTCGACTCTATACCACCAAATAACCCGTGGACTCTAATTCACCTCAACTCCGGTGTCTACAACTGTATACTGTCAATTGGAACCCTATGTGCTAATATAACCATATTCAGTGAAAAAGTTGAGATCAAGAAGCCATATATTATTCTTGAAGGAGACCGCGATCAGCAAACTGTGATTCAATATGGAGATGGCGGAAATGTTGTTACCAGTCCAACATTTAAGTTGAATGCGGATAATTTTGTGGCACGATATATTGTATTCAAGAATACCTATGATAATCTTCCTTCTAAATTAGACGCCAGCGGGGTCAACACTACTTGGGCACCTGCAGCTGCAATTAACGGAGACAGAGCAAGTTTTTACCATTGCAGCTTCAGTAGCTTGCAGGACACATTGACTGATGCCAAAGGTCGTCATTACTTCTATGACTGCAAAATTTCCGGCGCCATAGACTTCATTTGGGGAAATGCACAATCAATTTACGAGAATTGCCAGATCAATTCATTGGCATCAAAAATAGCTCCACGGGTAGGTTTTATTACAGCACAGGCTCGTGAAAGTCCAGAGGAAAATACTGGTTTTGTATTCAAAAATTGTCATGTGACTGGATCTGGCCCTATGTATCTAGGAAGAGCTCATACAAACCACGCAAGAGTATTGTTTGCTGGGACAACAATGGACAATGTTATTAGGGCAGAAGGTTGGGACGGGGCACGGTTTAAGGGCTCCGAGAATTTAATCTCCTTCTCGGAGGCCAATTGCAGGGGACCTGGAGCAAACATGTCGAAACGTGTAAAGTGGGAGAAGAAACTATCAGATCAAGAAGTAAATGATTTGACTAGTTTATCTTACATAAATCAAGATGGATGGATAGAGAAGCAACCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

346

Amino Acids

38.23

Weight (kDa)

6.99

Isoelectric Point (pI)

24.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 34 - 337 1.3e-50 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 484
Acc36I ACCTGC 1 cut(s) 484
AccB1I GGYRCC 2 cut(s) 472, 593
AccI GTMKAC 3 cut(s) 153, 207, 217
AccII CGCG 1 cut(s) 309
AciI CCGC 4 cut(s) 307, 342, 380, 453
AclWI GGATC 1 cut(s) 766
AcsI RAATTY 2 cut(s) 585, 883
AcuI CTGAAG 1 cut(s) 508
AcyI GRCGYC 2 cut(s) 447, 594
AfiI CCNNNNNNNGG 2 cut(s) 680, 912
AflIII ACRYGT 2 cut(s) 930, 940
AgsI TTSAA 8 cut(s) 64, 93, 143, 299, 329, 376, 412, 739
AhlI ACTAGT 1 cut(s) 989
AjnI CCWGG 1 cut(s) 919
AluBI AGCT 5 cut(s) 482, 522, 531, 671, 785
AluI AGCT 5 cut(s) 482, 522, 531, 671, 785
Alw21I GWGCWC 1 cut(s) 787
Alw26I GTCTC 3 cut(s) 12, 297, 489
AlwI GGATC 1 cut(s) 766
AoxI GGCC 2 cut(s) 763, 903
ApeKI GCWGC 3 cut(s) 479, 482, 519
ApoI RAATTY 2 cut(s) 585, 883
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 2 cut(s) 764, 917
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 917
BaeGI GKGCMC 2 cut(s) 475, 865
BanI GGYRCC 2 cut(s) 472, 593
BanII GRGCYC 2 cut(s) 787, 878
BarI GAAGNNNNNNTAC 2 cut(s) 772, 804
BauI CACGAG 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 787
BbvI GCAGC 3 cut(s) 469, 491, 531
BccI CCATC 4 cut(s) 107, 332, 1007, 1011
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 915, 949
BciT130I CCWGG 1 cut(s) 921
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 3 cut(s) 12, 297, 489
BcuI ACTAGT 1 cut(s) 989
BfaI CTAG 4 cut(s) 80, 776, 990, 1035
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 484
BisI GCNGC 3 cut(s) 480, 483, 520
BlsI GCNGC 3 cut(s) 481, 484, 521
Bme1390I CCNGG 1 cut(s) 921
Bme18I GGWCC 1 cut(s) 917
BmgT120I GGNCC 2 cut(s) 764, 917
BmiI GGNNCC 5 cut(s) 232, 474, 595, 877, 918
BmrFI CCNGG 1 cut(s) 921
BmsI GCATC 2 cut(s) 541, 668
BpmI CTGGAG 1 cut(s) 942
BsaHI GRCGYC 2 cut(s) 447, 594
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 2 cut(s) 176, 674
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 2 cut(s) 680, 912
Bse1I ACTGG 3 cut(s) 357, 730, 760
Bse3DI GCAATG 1 cut(s) 514
BseBI CCWGG 1 cut(s) 921
BseDI CCNNGG 2 cut(s) 176, 674
BseGI GGATG 1 cut(s) 1022
BseLI CCNNNNNNNGG 2 cut(s) 680, 912
BseMI GCAATG 1 cut(s) 514
BseNI ACTGG 3 cut(s) 357, 730, 760
BseSI GKGCMC 2 cut(s) 475, 865
BseXI GCAGC 3 cut(s) 469, 491, 531
BseYI CCCAGC 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 2 cut(s) 765, 905
BshNI GGYRCC 2 cut(s) 472, 593
BsiHKAI GWGCWC 1 cut(s) 787
BsiSI CCGG 2 cut(s) 201, 591
BslFI GGGAC 3 cut(s) 832, 869, 930
BslI CCNNNNNNNGG 2 cut(s) 680, 912
BsmAI GTCTC 3 cut(s) 12, 297, 489
BsmFI GGGAC 3 cut(s) 832, 869, 930
BsmI GAATGC 3 cut(s) 68, 70, 382
BsnI GGCC 2 cut(s) 765, 905
Bso31I GGTCTC 1 cut(s) 297
Bsp1286I GDGCHC 4 cut(s) 475, 787, 865, 878
Bsp143I GATC 6 cut(s) 109, 273, 310, 645, 758, 967
BspACI CCGC 4 cut(s) 307, 342, 380, 453
BspANI GGCC 2 cut(s) 765, 905
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 5 cut(s) 232, 474, 595, 877, 918
BspMAI CTGCAG 1 cut(s) 481
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 1 cut(s) 766
BspQI GCTCTTC 1 cut(s) 775
BspT107I GGYRCC 2 cut(s) 472, 593
BspTNI GGTCTC 1 cut(s) 297
BsrDI GCAATG 1 cut(s) 514
BsrI ACTGG 3 cut(s) 357, 730, 760
BssECI CCNNGG 2 cut(s) 176, 674
BssMI GATC 6 cut(s) 109, 273, 310, 645, 758, 967
BssNAI GTATAC 1 cut(s) 218
BssNI GRCGYC 2 cut(s) 447, 594
BssSI CACGAG 1 cut(s) 702
Bst1107I GTATAC 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 921
Bst4CI ACNGT 5 cut(s) 139, 215, 222, 322, 867
Bst6I CTCTTC 1 cut(s) 775
BstACI GRCGYC 2 cut(s) 447, 594
BstC8I GCNNGC 2 cut(s) 451, 533
BstDSI CCRYGG 2 cut(s) 176, 674
BstF5I GGATG 1 cut(s) 1022
BstFNI CGCG 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 6 cut(s) 112, 276, 313, 648, 761, 970
BstMAI GTCTC 3 cut(s) 12, 297, 489
BstMBI GATC 6 cut(s) 109, 273, 310, 645, 758, 967
BstMWI GCNNNNNNNGC 2 cut(s) 479, 528
BstNI CCWGG 1 cut(s) 921
BstNSI RCATGY 1 cut(s) 934
BstSCI CCNGG 1 cut(s) 919
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 2 cut(s) 475, 865
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 3 cut(s) 469, 491, 531
BstX2I RGATCY 1 cut(s) 758
BstYI RGATCY 1 cut(s) 758
BstZ17I GTATAC 1 cut(s) 218
BsuRI GGCC 2 cut(s) 765, 905
BtgI CCRYGG 2 cut(s) 176, 674
BtsCI GGATG 1 cut(s) 1022
BtsIMutI CAGTG 3 cut(s) 33, 135, 265
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 2 cut(s) 451, 533
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 2 cut(s) 764, 917
CseI GACGC 1 cut(s) 455
CviAII CATG 2 cut(s) 749, 931
DinI GGCGCC 1 cut(s) 595
DpnI GATC 6 cut(s) 111, 275, 312, 647, 760, 969
DpnII GATC 6 cut(s) 109, 273, 310, 645, 758, 967
Eam1104I CTCTTC 1 cut(s) 775
EarI CTCTTC 1 cut(s) 775
EciI GGCGGA 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 785
Eco24I GRGCYC 2 cut(s) 787, 878
Eco31I GGTCTC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 917
Eco53kI GAGCTC 1 cut(s) 785
Eco57I CTGAAG 1 cut(s) 508
EcoICRI GAGCTC 1 cut(s) 785
EcoO109I RGGNCCY 1 cut(s) 917
EcoRII CCWGG 1 cut(s) 919
EcoT22I ATGCAT 1 cut(s) 70
EcoT38I GRGCYC 2 cut(s) 787, 878
EgeI GGCGCC 1 cut(s) 595
EheI GGCGCC 1 cut(s) 595
FaeI CATG 2 cut(s) 752, 934
FaqI GGGAC 3 cut(s) 832, 869, 930
FatI CATG 2 cut(s) 748, 930
FauI CCCGC 1 cut(s) 446
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 3 cut(s) 153, 207, 217
Fnu4HI GCNGC 3 cut(s) 480, 483, 520
FokI GGATG 1 cut(s) 1029
FriOI GRGCYC 2 cut(s) 787, 878
Fsp4HI GCNGC 3 cut(s) 480, 483, 520
FspBI CTAG 4 cut(s) 80, 776, 990, 1035
GlaI GCGC 1 cut(s) 595
GluI GCNGC 3 cut(s) 480, 483, 520
GsaI CCCAGC 1 cut(s) 819
GsuI CTGGAG 1 cut(s) 942
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 2 cut(s) 765, 905
HapII CCGG 2 cut(s) 201, 591
HgaI GACGC 1 cut(s) 455
HhaI GCGC 1 cut(s) 596
Hin1I GRCGYC 2 cut(s) 447, 594
Hin1II CATG 2 cut(s) 752, 934
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HincII GTYRAC 2 cut(s) 154, 460
HindII GTYRAC 2 cut(s) 154, 460
HinfI GANTC 3 cut(s) 155, 181, 325
HpaII CCGG 2 cut(s) 201, 591
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 6 cut(s) 77, 154, 180, 208, 218, 460
Hpy188I TCNGA 5 cut(s) 54, 114, 880, 901, 967
Hpy188III TCNNGA 7 cut(s) 277, 296, 412, 704, 713, 971, 1010
Hpy8I GTNNAC 6 cut(s) 77, 154, 180, 208, 218, 460
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 4 cut(s) 293, 444, 842, 904
HpyCH4III ACNGT 5 cut(s) 139, 215, 222, 322, 867
HpyCH4IV ACGT 1 cut(s) 940
HpyCH4V TGCA 8 cut(s) 68, 479, 485, 519, 535, 582, 620, 912
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 528
HpySE526I ACGT 1 cut(s) 940
Hsp92I GRCGYC 2 cut(s) 447, 594
Hsp92II CATG 2 cut(s) 752, 934
HspAI GCGC 1 cut(s) 594
KasI GGCGCC 1 cut(s) 593
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 6 cut(s) 109, 273, 310, 645, 758, 967
LguI GCTCTTC 1 cut(s) 775
LmnI GCTCC 3 cut(s) 676, 881, 923
Lsp1109I GCAGC 3 cut(s) 469, 491, 531
LweI GCATC 2 cut(s) 541, 668
MaeI CTAG 4 cut(s) 80, 776, 990, 1035
MaeII ACGT 1 cut(s) 940
MaeIII GTNAC 2 cut(s) 352, 751
MalI GATC 6 cut(s) 111, 275, 312, 647, 760, 969
MboI GATC 6 cut(s) 109, 273, 310, 645, 758, 967
MboII GAAGA 3 cut(s) 422, 792, 967
MfeI CAATTG 2 cut(s) 225, 907
MflI RGATCY 1 cut(s) 758
MhlI GDGCHC 4 cut(s) 475, 787, 865, 878
Mly113I GGCGCC 1 cut(s) 594
MlyI GAGTC 2 cut(s) 149, 175
MmeI TCCRAC 1 cut(s) 386
MnlI CCTC 3 cut(s) 203, 709, 895
Mph1103I ATGCAT 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 4 cut(s) 369, 489, 870, 887
MspA1I CMGCKG 2 cut(s) 453, 482
MspI CCGG 2 cut(s) 201, 591
MspR9I CCNGG 1 cut(s) 921
MunI CAATTG 2 cut(s) 225, 907
Mva1269I GAATGC 3 cut(s) 68, 70, 382
MvaI CCWGG 1 cut(s) 921
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 2 cut(s) 479, 528
NarI GGCGCC 1 cut(s) 594
NdeII GATC 6 cut(s) 109, 273, 310, 645, 758, 967
NlaIII CATG 2 cut(s) 752, 934
NlaIV GGNNCC 5 cut(s) 232, 474, 595, 877, 918
NmuCI GTSAC 1 cut(s) 751
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 1 cut(s) 934
PaqCI CACCTGC 1 cut(s) 484
PciI ACATGT 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 775
PctI GAATGC 3 cut(s) 68, 70, 382
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 1 cut(s) 325
PkrI GCNGC 3 cut(s) 481, 484, 521
PleI GAGTC 2 cut(s) 149, 175
PluTI GGCGCC 1 cut(s) 597
PpsI GAGTC 2 cut(s) 149, 175
PpuMI RGGWCCY 1 cut(s) 917
PscI ACATGT 1 cut(s) 930
Psp124BI GAGCTC 1 cut(s) 787
Psp5II RGGWCCY 1 cut(s) 917
Psp6I CCWGG 1 cut(s) 919
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 1 cut(s) 919
PspN4I GGNNCC 5 cut(s) 232, 474, 595, 877, 918
PspPI GGNCC 2 cut(s) 764, 917
PspPPI RGGWCCY 1 cut(s) 917
PstI CTGCAG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 758
PvuII CAGCTG 1 cut(s) 482
SacI GAGCTC 1 cut(s) 787
SalI GTCGAC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 775
SaqAI TTAA 4 cut(s) 369, 489, 870, 887
SatI GCNGC 3 cut(s) 480, 483, 520
Sau3AI GATC 6 cut(s) 109, 273, 310, 645, 758, 967
Sau96I GGNCC 2 cut(s) 764, 917
SchI GAGTC 2 cut(s) 149, 175
ScrFI CCNGG 1 cut(s) 921
SduI GDGCHC 4 cut(s) 475, 787, 865, 878
SfaNI GCATC 2 cut(s) 541, 668
SfcI CTRYAG 1 cut(s) 477
SfoI GGCGCC 1 cut(s) 595
SinI GGWCC 1 cut(s) 917
SpeI ACTAGT 1 cut(s) 989
SsiI CCGC 4 cut(s) 307, 342, 380, 453
SspDI GGCGCC 1 cut(s) 593
SspMI CTAG 4 cut(s) 80, 776, 990, 1035
SstI GAGCTC 1 cut(s) 787
StyD4I CCNGG 1 cut(s) 919
TaaI ACNGT 5 cut(s) 139, 215, 222, 322, 867
TaiI ACGT 1 cut(s) 943
TaqI TCGA 2 cut(s) 153, 935
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 4 cut(s) 369, 489, 870, 887
Tru9I TTAA 4 cut(s) 369, 489, 870, 887
TscAI CASTG 3 cut(s) 33, 142, 265
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 3 cut(s) 479, 482, 519
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 3 cut(s) 17, 595, 642
TspGWI ACGGA 2 cut(s) 62, 507
TspRI CASTG 3 cut(s) 33, 142, 265
VpaK11BI GGWCC 1 cut(s) 917
XapI RAATTY 2 cut(s) 585, 883
XceI RCATGY 1 cut(s) 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XmiI GTMKAC 3 cut(s) 153, 207, 217
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 4 cut(s) 80, 776, 990, 1035
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.