Rroxscaffold_2G00134660

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001788
Physical Location & Seq
Forward (+)
72206664 .. 72207648
985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134660.1

Sequence Viewer

Length: 699 bp
ATGGTCGAGGGCAATTTAGCAGTGTACAACAAGCCATTGATTCCATACCATCAAATAATGCATGGTGGATCCGGATCCATATTAACCATGGTGTCTACTAGTTACAATCCTCAAGACAAACCGTACATTATTCTTGAAGGAAACCCTAATCATCTTACAACAATCGAATTTGGAGATGCTGGCAGTTTTGTTGACAGTCCTACATTCAAGGCACATGCCGATAATTTTGTGGCAAAAAATATAGTATTCAAGAATACTTGCGACCACCTTATCACATTGGAGCCGAATGGGAGAAAAACTACTTGGGCAACTGCTGCTGCAATAGATGGAGACAAAGCAAGTTTCTATAACTGCACTTTCATTAGCTTGCAAGATACACTGTCTGATGCCCAAGGTCGACACTATTTCTATGATTTCAACATTCAAGGTGCTATAGACTTTATTTGGGGAAATGGCCAGTCAATTTACGAGAAATGTCAGATAACTTCGTTAGTCCACAAACTAGGCCAGAATGGTGGTTATATCACTGCACAGGGTCGCGAAAGACCTGAGGAAACTACTGGGTTTGTATTCAAAGATTGCCATGTATGTGGATCAGGACCTATAATGCTAGGAAGAGCATACAGAGCATACGCAAGAGTATTGTTTTCTGGGACATATATGGAAAACATTATTACACCCCAGGGCTGGTCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

232

Amino Acids

25.71

Weight (kDa)

5.88

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 44 - 232 8.6e-34 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 95, 397
AccII CGCG 1 cut(s) 540
AccIII TCCGGA 1 cut(s) 71
AclWI GGATC 5 cut(s) 63, 69, 76, 82, 601
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 1 cut(s) 167
AfaI GTAC 2 cut(s) 26, 125
AfiI CCNNNNNNNGG 1 cut(s) 687
AgsI TTSAA 6 cut(s) 137, 208, 250, 418, 425, 574
AhlI ACTAGT 1 cut(s) 98
AjnI CCWGG 1 cut(s) 681
AjuI GAANNNNNNNTTGG 2 cut(s) 286, 318
AluBI AGCT 1 cut(s) 366
AluI AGCT 1 cut(s) 366
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 5 cut(s) 63, 69, 76, 82, 601
Aor13HI TCCGGA 1 cut(s) 71
AoxI GGCC 2 cut(s) 454, 505
ApeKI GCWGC 2 cut(s) 314, 317
ApoI RAATTY 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 599
AvaII GGWCC 1 cut(s) 599
AxyI CCTNAGG 1 cut(s) 549
BalI TGGCCA 1 cut(s) 456
BamHI GGATCC 2 cut(s) 68, 74
BbvI GCAGC 2 cut(s) 301, 304
BccI CCATC 2 cut(s) 57, 320
BciT130I CCWGG 1 cut(s) 683
BcoDI GTCTC 1 cut(s) 324
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 3 cut(s) 99, 503, 611
BfmI CTRYAG 1 cut(s) 432
BisI GCNGC 2 cut(s) 315, 318
BlsI GCNGC 2 cut(s) 316, 319
Bme1390I CCNGG 1 cut(s) 683
Bme18I GGWCC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 3 cut(s) 70, 76, 282
BmrFI CCNGG 1 cut(s) 683
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 2 cut(s) 166, 376
BmuI ACTGGG 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 73
BsaJI CCNNGG 4 cut(s) 87, 391, 681, 682
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 1 cut(s) 687
Bse1I ACTGG 2 cut(s) 457, 565
Bse21I CCTNAGG 1 cut(s) 549
Bse8I GATNNNNATC 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 71
BseBI CCWGG 1 cut(s) 683
BseDI CCNNGG 4 cut(s) 87, 391, 681, 682
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 2 cut(s) 457, 565
BseXI GCAGC 2 cut(s) 301, 304
BsgI GTGCAG 2 cut(s) 337, 513
Bsh1236I CGCG 1 cut(s) 540
BshFI GGCC 2 cut(s) 456, 507
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 1 cut(s) 687
BsmAI GTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 667
BsnI GGCC 2 cut(s) 456, 507
Bsp13I TCCGGA 1 cut(s) 71
Bsp1407I TGTACA 1 cut(s) 24
Bsp143I GATC 3 cut(s) 68, 74, 593
Bsp19I CCATGG 1 cut(s) 87
Bsp68I TCGCGA 1 cut(s) 540
BspANI GGCC 2 cut(s) 456, 507
BspCNI CTCAG 1 cut(s) 541
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 3 cut(s) 70, 76, 282
BspPI GGATC 5 cut(s) 63, 69, 76, 82, 601
BspQI GCTCTTC 1 cut(s) 610
BsrGI TGTACA 1 cut(s) 24
BsrI ACTGG 2 cut(s) 457, 565
BssECI CCNNGG 4 cut(s) 87, 391, 681, 682
BssMI GATC 3 cut(s) 68, 74, 593
BssT1I CCWWGG 2 cut(s) 87, 391
Bst2UI CCWGG 1 cut(s) 683
Bst4CI ACNGT 3 cut(s) 123, 197, 381
Bst6I CTCTTC 1 cut(s) 610
BstAPI GCANNNNNTGC 1 cut(s) 314
BstAUI TGTACA 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 181, 368
BstDEI CTNAG 1 cut(s) 549
BstDSI CCRYGG 1 cut(s) 87
BstFNI CGCG 1 cut(s) 540
BstKTI GATC 3 cut(s) 71, 77, 596
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 3 cut(s) 68, 74, 593
BstMWI GCNNNNNNNGC 2 cut(s) 314, 626
BstNI CCWGG 1 cut(s) 683
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 681
BstSFI CTRYAG 1 cut(s) 432
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 2 cut(s) 301, 304
BstX2I RGATCY 2 cut(s) 68, 74
BstXI CCANNNNNNTGG 2 cut(s) 515, 590
BstYI RGATCY 2 cut(s) 68, 74
Bsu36I CCTNAGG 1 cut(s) 549
BsuRI GGCC 2 cut(s) 456, 507
BtgI CCRYGG 1 cut(s) 87
BtsI GCAGTG 2 cut(s) 27, 525
BtsIMutI CAGTG 3 cut(s) 27, 377, 525
BtuMI TCGCGA 1 cut(s) 540
Cac8I GCNNGC 2 cut(s) 181, 368
Cfr13I GGNCC 1 cut(s) 599
Csp6I GTAC 2 cut(s) 25, 124
CviAII CATG 4 cut(s) 62, 88, 215, 584
CviJI RGCY 6 cut(s) 34, 283, 366, 456, 507, 687
CviKI_1 RGCY 6 cut(s) 34, 283, 366, 456, 507, 687
CviQI GTAC 2 cut(s) 25, 124
DdeI CTNAG 1 cut(s) 549
DpnI GATC 3 cut(s) 70, 76, 595
DpnII GATC 3 cut(s) 68, 74, 593
EaeI YGGCCR 1 cut(s) 454
Eam1104I CTCTTC 1 cut(s) 610
EarI CTCTTC 1 cut(s) 610
Eco130I CCWWGG 2 cut(s) 87, 391
Eco47I GGWCC 1 cut(s) 599
Eco81I CCTNAGG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 599
EcoRII CCWGG 1 cut(s) 681
EcoT14I CCWWGG 2 cut(s) 87, 391
EcoT22I ATGCAT 1 cut(s) 63
ErhI CCWWGG 2 cut(s) 87, 391
FaeI CATG 4 cut(s) 65, 91, 218, 587
FaqI GGGAC 1 cut(s) 667
FatI CATG 4 cut(s) 61, 87, 214, 583
FblI GTMKAC 2 cut(s) 95, 397
Fnu4HI GCNGC 2 cut(s) 315, 318
Fsp4HI GCNGC 2 cut(s) 315, 318
FspBI CTAG 3 cut(s) 99, 503, 611
GluI GCNGC 2 cut(s) 315, 318
HaeIII GGCC 2 cut(s) 456, 507
HapII CCGG 1 cut(s) 72
Hin1II CATG 4 cut(s) 65, 91, 218, 587
HincII GTYRAC 2 cut(s) 193, 398
HindII GTYRAC 2 cut(s) 193, 398
HinfI GANTC 1 cut(s) 40
HpaII CCGG 1 cut(s) 72
Hpy166II GTNNAC 5 cut(s) 25, 96, 193, 398, 496
Hpy188I TCNGA 3 cut(s) 385, 480, 694
Hpy188III TCNNGA 6 cut(s) 72, 113, 134, 250, 539, 597
Hpy8I GTNNAC 5 cut(s) 25, 96, 193, 398, 496
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 3 cut(s) 123, 197, 381
HpyCH4V TGCA 5 cut(s) 61, 320, 354, 370, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 626
HpyF3I CTNAG 1 cut(s) 549
Hsp92II CATG 4 cut(s) 65, 91, 218, 587
Kpn2I TCCGGA 1 cut(s) 71
Kzo9I GATC 3 cut(s) 68, 74, 593
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 2 cut(s) 301, 304
LweI GCATC 2 cut(s) 166, 376
MaeI CTAG 3 cut(s) 99, 503, 611
MaeIII GTNAC 1 cut(s) 101
MalI GATC 3 cut(s) 70, 76, 595
MboI GATC 3 cut(s) 68, 74, 593
MboII GAAGA 1 cut(s) 627
MflI RGATCY 2 cut(s) 68, 74
MlsI TGGCCA 1 cut(s) 456
MluCI AATT 4 cut(s) 13, 167, 223, 462
MluNI TGGCCA 1 cut(s) 456
MnlI CCTC 2 cut(s) 120, 544
Mox20I TGGCCA 1 cut(s) 456
Mph1103I ATGCAT 1 cut(s) 63
MroI TCCGGA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 456
MseI TTAA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 588
Msp20I TGGCCA 1 cut(s) 456
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 1 cut(s) 683
MvaI CCWGG 1 cut(s) 683
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 2 cut(s) 314, 626
NcoI CCATGG 1 cut(s) 87
NdeII GATC 3 cut(s) 68, 74, 593
NlaIII CATG 4 cut(s) 65, 91, 218, 587
NlaIV GGNNCC 3 cut(s) 70, 76, 282
NruI TCGCGA 1 cut(s) 540
NsiI ATGCAT 1 cut(s) 63
NspI RCATGY 1 cut(s) 218
PasI CCCWGGG 1 cut(s) 682
PciSI GCTCTTC 1 cut(s) 610
PfeI GAWTC 1 cut(s) 40
PkrI GCNGC 2 cut(s) 316, 319
PpuMI RGGWCCY 1 cut(s) 599
Psp5II RGGWCCY 1 cut(s) 599
Psp6I CCWGG 1 cut(s) 681
PspGI CCWGG 1 cut(s) 681
PspN4I GGNNCC 3 cut(s) 70, 76, 282
PspPI GGNCC 1 cut(s) 599
PspPPI RGGWCCY 1 cut(s) 599
PsuI RGATCY 2 cut(s) 68, 74
RruI TCGCGA 1 cut(s) 540
RsaI GTAC 2 cut(s) 26, 125
RsaNI GTAC 2 cut(s) 25, 124
RseI CAYNNNNRTG 1 cut(s) 588
SalI GTCGAC 1 cut(s) 396
SapI GCTCTTC 1 cut(s) 610
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 2 cut(s) 315, 318
Sau3AI GATC 3 cut(s) 68, 74, 593
Sau96I GGNCC 1 cut(s) 599
ScrFI CCNGG 1 cut(s) 683
SetI ASST 6 cut(s) 270, 368, 397, 430, 550, 604
SfaNI GCATC 2 cut(s) 166, 376
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SpeI ACTAGT 1 cut(s) 98
Sse9I AATT 4 cut(s) 13, 167, 223, 462
SspMI CTAG 3 cut(s) 99, 503, 611
StyD4I CCNGG 1 cut(s) 681
StyI CCWWGG 2 cut(s) 87, 391
TaaI ACNGT 3 cut(s) 123, 197, 381
TaqI TCGA 3 cut(s) 6, 165, 397
TasI AATT 4 cut(s) 13, 167, 223, 462
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 1 cut(s) 40
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 3 cut(s) 27, 384, 532
TseI GCWGC 2 cut(s) 314, 317
TspDTI ATGAA 1 cut(s) 349
TspRI CASTG 3 cut(s) 27, 384, 532
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 218
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmiI GTMKAC 2 cut(s) 95, 397
XspI CTAG 3 cut(s) 99, 503, 611
Zsp2I ATGCAT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.