Rh2CG210800

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001788
Physical Location & Seq
Reverse (-)
20655713 .. 20673915
18203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG210800.1

Sequence Viewer

Length: 603 bp
ATGTCTGCTATAACAAAAGGTCACCCTAAATATCTCACTGCAATAGAATTTGGAGACTTTGGCAGTGTTTCAAAGAGTCCTACATTTACGTTACTTGCAGATAATTTCGTAGCTCGGAATATTCTATTCAAGAATTCATATGACCATCTTATCAAAGTGGATCCTAATAACCGGAGAACTACTTGGGCAGTTGCGGCTAAAATTGTTGGAGACAAAGCAAGTTTTTATCATTGTGGTTTCATTAGCTTGCAAGATACACTGTGGGATGCCAAAGGTCGTCACTATTTCTATGATTGCGATGTTCGTGGTGCTATAGATTTTATTTGGGGCAATGGCCAGTCAATTTATGAGAAATGTCAGATAATGTCTTTGAGCAAATTAATTGGCACTACTGGCTATATTACAGCACAAGGTCGTGAAGGTCCAGACGAAACTACTGGTTTTGTATTCAAAGATTGTTATGTATGTGGATCGGGCACTGCATTTCTTGGGAGAGCATACAGAGACTACTCAAGAGTCTTGTTTTCAGGGACGTATATGGAAAATGTTATTACCCCGGAGGGTTGGTCCCTTTGGACACCGCCACAAATTCAACATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

200

Amino Acids

22.44

Weight (kDa)

7.64

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 23 - 193 4.3e-38 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 194, 581
AclWI GGATC 3 cut(s) 155, 168, 478
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 3 cut(s) 47, 133, 588
AgsI TTSAA 4 cut(s) 72, 130, 451, 593
AluBI AGCT 2 cut(s) 113, 246
AluI AGCT 2 cut(s) 113, 246
Alw26I GTCTC 3 cut(s) 48, 204, 498
AlwI GGATC 3 cut(s) 155, 168, 478
AoxI GGCC 1 cut(s) 334
ApoI RAATTY 3 cut(s) 47, 133, 588
AseI ATTAAT 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 422, 567
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 1 cut(s) 14
AvaII GGWCC 2 cut(s) 422, 567
BaeGI GKGCMC 1 cut(s) 479
BalI TGGCCA 1 cut(s) 336
BamHI GGATCC 1 cut(s) 160
BccI CCATC 1 cut(s) 153
BcnI CCSGG 1 cut(s) 557
BcoDI GTCTC 3 cut(s) 48, 204, 498
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 557
Bme18I GGWCC 2 cut(s) 422, 567
BmgT120I GGNCC 2 cut(s) 422, 567
BmiI GGNNCC 2 cut(s) 162, 569
BmrFI CCNGG 1 cut(s) 557
BmsI GCATC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 496
BpuMI CCSGG 1 cut(s) 557
BsaJI CCNNGG 1 cut(s) 555
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bse1I ACTGG 3 cut(s) 337, 397, 442
Bse3DI GCAATG 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 555
BseGI GGATG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 337
BseNI ACTGG 3 cut(s) 337, 397, 442
BseSI GKGCMC 1 cut(s) 479
BshFI GGCC 1 cut(s) 336
BsiSI CCGG 2 cut(s) 172, 557
BslFI GGGAC 2 cut(s) 544, 553
BsmAI GTCTC 3 cut(s) 48, 204, 498
BsmFI GGGAC 2 cut(s) 544, 553
BsnI GGCC 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 479
Bsp143I GATC 2 cut(s) 160, 470
BspACI CCGC 2 cut(s) 194, 581
BspANI GGCC 1 cut(s) 336
BspLI GGNNCC 2 cut(s) 162, 569
BspPI GGATC 3 cut(s) 155, 168, 478
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 3 cut(s) 337, 397, 442
BssECI CCNNGG 1 cut(s) 555
BssMI GATC 2 cut(s) 160, 470
Bst4CI ACNGT 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 248
BstEII GGTNACC 1 cut(s) 20
BstF5I GGATG 1 cut(s) 271
BstKTI GATC 2 cut(s) 163, 473
BstMAI GTCTC 3 cut(s) 48, 204, 498
BstMBI GATC 2 cut(s) 160, 470
BstMWI GCNNNNNNNGC 2 cut(s) 194, 393
BstNSI RCATGY 1 cut(s) 599
BstPI GGTNACC 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 555
BstSFI CTRYAG 1 cut(s) 312
BstSLI GKGCMC 1 cut(s) 479
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 3 cut(s) 36, 70, 477
BtsIMutI CAGTG 4 cut(s) 36, 70, 257, 477
Cac8I GCNNGC 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 422, 567
CviAII CATG 1 cut(s) 596
CviJI RGCY 5 cut(s) 113, 197, 246, 336, 396
CviKI_1 RGCY 5 cut(s) 113, 197, 246, 336, 396
DpnI GATC 2 cut(s) 162, 472
DpnII GATC 2 cut(s) 160, 470
EaeI YGGCCR 1 cut(s) 334
Eco47I GGWCC 2 cut(s) 422, 567
Eco91I GGTNACC 1 cut(s) 20
EcoO65I GGTNACC 1 cut(s) 20
EcoRI GAATTC 1 cut(s) 133
FaeI CATG 1 cut(s) 599
FaqI GGGAC 2 cut(s) 544, 553
FatI CATG 1 cut(s) 595
FauNDI CATATG 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 1 cut(s) 195
GluI GCNGC 1 cut(s) 195
HaeIII GGCC 1 cut(s) 336
HapII CCGG 2 cut(s) 172, 557
Hin1II CATG 1 cut(s) 599
HinfI GANTC 2 cut(s) 76, 516
HpaII CCGG 2 cut(s) 172, 557
HphI GGTGA 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 117, 360
Hpy188III TCNNGA 4 cut(s) 130, 416, 425, 513
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 1 cut(s) 261
HpyCH4IV ACGT 2 cut(s) 89, 533
HpyCH4V TGCA 4 cut(s) 41, 98, 250, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 393
HpySE526I ACGT 2 cut(s) 89, 533
Hsp92II CATG 1 cut(s) 599
Kzo9I GATC 2 cut(s) 160, 470
LpnPI CCDG 7 cut(s) 185, 350, 378, 423, 438, 513, 570
LweI GCATC 1 cut(s) 256
MaeII ACGT 2 cut(s) 89, 533
MaeIII GTNAC 3 cut(s) 20, 90, 278
MalI GATC 2 cut(s) 162, 472
MboI GATC 2 cut(s) 160, 470
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 1 cut(s) 479
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 8 cut(s) 47, 103, 133, 201, 342, 377, 381, 588
MluNI TGGCCA 1 cut(s) 336
MlyI GAGTC 2 cut(s) 85, 525
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 1 cut(s) 553
Mox20I TGGCCA 1 cut(s) 336
MscI TGGCCA 1 cut(s) 336
MseI TTAA 1 cut(s) 380
Msp20I TGGCCA 1 cut(s) 336
MspI CCGG 2 cut(s) 172, 557
MspR9I CCNGG 1 cut(s) 557
MwoI GCNNNNNNNGC 2 cut(s) 194, 393
NciI CCSGG 1 cut(s) 557
NdeI CATATG 1 cut(s) 139
NdeII GATC 2 cut(s) 160, 470
NlaIII CATG 1 cut(s) 599
NlaIV GGNNCC 2 cut(s) 162, 569
NmuCI GTSAC 2 cut(s) 20, 278
NspI RCATGY 1 cut(s) 599
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 2 cut(s) 84, 524
PpsI GAGTC 2 cut(s) 84, 524
PshBI ATTAAT 1 cut(s) 380
PspEI GGTNACC 1 cut(s) 20
PspN4I GGNNCC 2 cut(s) 162, 569
PspPI GGNCC 2 cut(s) 422, 567
PsuI RGATCY 1 cut(s) 160
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 2 cut(s) 160, 470
Sau96I GGNCC 2 cut(s) 422, 567
SchI GAGTC 2 cut(s) 85, 525
ScrFI CCNGG 1 cut(s) 557
SduI GDGCHC 1 cut(s) 479
SetI ASST 8 cut(s) 22, 92, 115, 248, 277, 415, 424, 536
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 422, 567
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 8 cut(s) 47, 103, 133, 201, 342, 377, 381, 588
SsiI CCGC 2 cut(s) 194, 581
SspI AATATT 1 cut(s) 121
StyD4I CCNGG 1 cut(s) 555
TaaI ACNGT 1 cut(s) 261
TaiI ACGT 2 cut(s) 92, 536
TasI AATT 8 cut(s) 47, 103, 133, 201, 342, 377, 381, 588
TauI GCSGC 1 cut(s) 197
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TscAI CASTG 4 cut(s) 43, 70, 264, 484
TseFI GTSAC 2 cut(s) 20, 278
Tsp45I GTSAC 2 cut(s) 20, 278
TspDTI ATGAA 2 cut(s) 126, 229
TspRI CASTG 4 cut(s) 43, 70, 264, 484
VpaK11BI GGWCC 2 cut(s) 422, 567
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 3 cut(s) 47, 133, 588
XceI RCATGY 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.