Rh2BG218900

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001788
Physical Location & Seq
Reverse (-)
21102152 .. 21119404
17253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG218900.1

Sequence Viewer

Length: 675 bp
ATGAAAGTACTGGTTTTGTTTTCAAAGATTGTCATGTATATGGATCAGGCACTTCATTTCTTGGAAGAGCATAGTACTGTTTTCCGGGACGTATATGGAAAACGTTATTACACCGGACGGGATTTAATCACATTTTCCGAGGATAACTGCACTGGACCTGGAGCAGACATGTCTCATCGTGTTCCATGGGAGAAGAAGTTATCGAATGAAGAAAATTCATATGACCATCTTATCAAAGTGGATCCTAATAACCGGAGAACTACTTGGGCAGTTGCGGCTAAAATTGTTGGAGACAAAGCAAGTTTTTATCATTGTGGTTTCATTAGCTTGCAAGATACACTGTGGGATGCCAAAGGTCGTCACTATTTCTATGATTGCGATGTTCGTGGTGCTATAGATTTTATTTGGGGCAATGGCCAGTCAATTTATGAGATAATGTCTTTGAGCAAATTAATTGGCACCACTGGCTATATTACAGCACAAGGTCGTGAAGGTCCAGACGAAACTACTGGTTTTGTATTCAAAGATTGTTATGTATGTGGATCGGGCACTGCATTTCTTGGGAGAGCATACAGAGACTACTCAAGAGTCTTGTTTTCAGGGACGTATATGGAAAATGTCATTACCCCGGAGGGTTGGTCCCTTTGGACACCGCCACAAATTCAACATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

224

Amino Acids

25.6

Weight (kDa)

5.96

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 89 - 217 3.4e-30 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 458
AciI CCGC 2 cut(s) 275, 653
AclI AACGTT 1 cut(s) 103
AclWI GGATC 4 cut(s) 51, 236, 249, 550
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 2 cut(s) 214, 660
AfaI GTAC 2 cut(s) 9, 76
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 3 cut(s) 24, 523, 665
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
Alw26I GTCTC 3 cut(s) 177, 285, 570
AlwI GGATC 4 cut(s) 51, 236, 249, 550
AoxI GGCC 1 cut(s) 415
ApoI RAATTY 2 cut(s) 214, 660
AseI ATTAAT 1 cut(s) 452
AspS9I GGNCC 3 cut(s) 155, 494, 639
AsuC2I CCSGG 2 cut(s) 86, 629
AvaII GGWCC 3 cut(s) 155, 494, 639
BaeGI GKGCMC 1 cut(s) 551
BalI TGGCCA 1 cut(s) 417
BamHI GGATCC 1 cut(s) 241
BanI GGYRCC 1 cut(s) 458
BccI CCATC 1 cut(s) 234
BciT130I CCWGG 1 cut(s) 159
BcnI CCSGG 2 cut(s) 86, 629
BcoDI GTCTC 3 cut(s) 177, 285, 570
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
BmcAI AGTACT 2 cut(s) 9, 76
Bme1390I CCNGG 3 cut(s) 86, 159, 629
Bme18I GGWCC 3 cut(s) 155, 494, 639
BmgT120I GGNCC 3 cut(s) 155, 494, 639
BmiI GGNNCC 3 cut(s) 243, 460, 641
BmrFI CCNGG 3 cut(s) 86, 159, 629
BmsI GCATC 1 cut(s) 337
BpmI CTGGAG 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 568
BpuMI CCSGG 2 cut(s) 86, 629
BsaJI CCNNGG 3 cut(s) 138, 185, 627
BsaWI WCCGGW 2 cut(s) 113, 252
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 623, 653
Bse1I ACTGG 5 cut(s) 15, 157, 418, 469, 514
Bse3DI GCAATG 1 cut(s) 418
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 138, 185, 627
BseGI GGATG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 418
BseNI ACTGG 5 cut(s) 15, 157, 418, 469, 514
BseSI GKGCMC 1 cut(s) 551
BsgI GTGCAG 1 cut(s) 133
BshFI GGCC 1 cut(s) 417
BshNI GGYRCC 1 cut(s) 458
BsiSI CCGG 4 cut(s) 85, 114, 253, 629
BslFI GGGAC 3 cut(s) 101, 616, 625
BsmAI GTCTC 3 cut(s) 177, 285, 570
BsmFI GGGAC 3 cut(s) 101, 616, 625
BsnI GGCC 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 551
Bsp143I GATC 3 cut(s) 43, 241, 542
Bsp19I CCATGG 1 cut(s) 185
BspACI CCGC 2 cut(s) 275, 653
BspANI GGCC 1 cut(s) 417
BspLI GGNNCC 3 cut(s) 243, 460, 641
BspPI GGATC 4 cut(s) 51, 236, 249, 550
BspQI GCTCTTC 1 cut(s) 60
BspT107I GGYRCC 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 418
BsrI ACTGG 5 cut(s) 15, 157, 418, 469, 514
BssECI CCNNGG 3 cut(s) 138, 185, 627
BssMI GATC 3 cut(s) 43, 241, 542
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 2 cut(s) 79, 342
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 329
BstDSI CCRYGG 1 cut(s) 185
BstF5I GGATG 1 cut(s) 352
BstKTI GATC 3 cut(s) 46, 244, 545
BstMAI GTCTC 3 cut(s) 177, 285, 570
BstMBI GATC 3 cut(s) 43, 241, 542
BstMWI GCNNNNNNNGC 2 cut(s) 275, 465
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 2 cut(s) 172, 671
BstSCI CCNGG 3 cut(s) 84, 157, 627
BstSFI CTRYAG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 1 cut(s) 417
BtgI CCRYGG 1 cut(s) 185
BtgZI GCGATG 1 cut(s) 393
BtsCI GGATG 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 4 cut(s) 150, 338, 462, 549
Cac8I GCNNGC 1 cut(s) 329
Cfr13I GGNCC 3 cut(s) 155, 494, 639
Csp6I GTAC 2 cut(s) 8, 75
CviAII CATG 4 cut(s) 34, 169, 186, 668
CviJI RGCY 4 cut(s) 278, 327, 417, 468
CviKI_1 RGCY 4 cut(s) 278, 327, 417, 468
CviQI GTAC 2 cut(s) 8, 75
DpnI GATC 3 cut(s) 45, 243, 544
DpnII GATC 3 cut(s) 43, 241, 542
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 185
Eco47I GGWCC 3 cut(s) 155, 494, 639
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 4 cut(s) 37, 172, 189, 671
FaqI GGGAC 3 cut(s) 101, 616, 625
FatI CATG 4 cut(s) 33, 168, 185, 667
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 1 cut(s) 276
GluI GCNGC 1 cut(s) 276
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 1 cut(s) 417
HapII CCGG 4 cut(s) 85, 114, 253, 629
Hin1II CATG 4 cut(s) 37, 172, 189, 671
HinfI GANTC 1 cut(s) 588
HpaII CCGG 4 cut(s) 85, 114, 253, 629
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 3 cut(s) 488, 497, 585
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 2 cut(s) 79, 342
HpyCH4IV ACGT 3 cut(s) 90, 103, 605
HpyCH4V TGCA 3 cut(s) 150, 331, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 465
HpySE526I ACGT 3 cut(s) 90, 103, 605
Hsp92II CATG 4 cut(s) 37, 172, 189, 671
Kzo9I GATC 3 cut(s) 43, 241, 542
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 1 cut(s) 161
LweI GCATC 1 cut(s) 337
MaeII ACGT 3 cut(s) 90, 103, 605
MaeIII GTNAC 1 cut(s) 359
MalI GATC 3 cut(s) 45, 243, 544
MboI GATC 3 cut(s) 43, 241, 542
MboII GAAGA 3 cut(s) 77, 205, 221
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 551
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 6 cut(s) 214, 282, 423, 449, 453, 660
MluNI TGGCCA 1 cut(s) 417
MlyI GAGTC 1 cut(s) 597
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 2 cut(s) 133, 625
Mox20I TGGCCA 1 cut(s) 417
MscI TGGCCA 1 cut(s) 417
MseI TTAA 2 cut(s) 125, 452
MslI CAYNNNNRTG 1 cut(s) 38
Msp20I TGGCCA 1 cut(s) 417
MspI CCGG 4 cut(s) 85, 114, 253, 629
MspR9I CCNGG 3 cut(s) 86, 159, 629
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 275, 465
NciI CCSGG 2 cut(s) 86, 629
NcoI CCATGG 1 cut(s) 185
NdeI CATATG 1 cut(s) 220
NdeII GATC 3 cut(s) 43, 241, 542
NlaIII CATG 4 cut(s) 37, 172, 189, 671
NlaIV GGNNCC 3 cut(s) 243, 460, 641
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 2 cut(s) 172, 671
PciI ACATGT 1 cut(s) 168
PciSI GCTCTTC 1 cut(s) 60
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
PscI ACATGT 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 452
Psp1406I AACGTT 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 3 cut(s) 243, 460, 641
PspPI GGNCC 3 cut(s) 155, 494, 639
PsuI RGATCY 1 cut(s) 241
RsaI GTAC 2 cut(s) 9, 76
RsaNI GTAC 2 cut(s) 8, 75
RseI CAYNNNNRTG 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 125, 452
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 3 cut(s) 43, 241, 542
Sau96I GGNCC 3 cut(s) 155, 494, 639
ScaI AGTACT 2 cut(s) 9, 76
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 3 cut(s) 86, 159, 629
SduI GDGCHC 1 cut(s) 551
SetI ASST 8 cut(s) 93, 106, 160, 329, 358, 487, 496, 608
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 3 cut(s) 155, 494, 639
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 6 cut(s) 214, 282, 423, 449, 453, 660
SsiI CCGC 2 cut(s) 275, 653
StyD4I CCNGG 3 cut(s) 84, 157, 627
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 2 cut(s) 79, 342
TaiI ACGT 3 cut(s) 93, 106, 608
TaqI TCGA 1 cut(s) 203
TasI AATT 6 cut(s) 214, 282, 423, 449, 453, 660
TatI WGTACW 2 cut(s) 7, 74
TauI GCSGC 1 cut(s) 278
Tru1I TTAA 2 cut(s) 125, 452
Tru9I TTAA 2 cut(s) 125, 452
TscAI CASTG 4 cut(s) 157, 345, 469, 556
TseFI GTSAC 1 cut(s) 359
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 5 cut(s) 17, 44, 207, 222, 310
TspRI CASTG 4 cut(s) 157, 345, 469, 556
VpaK11BI GGWCC 3 cut(s) 155, 494, 639
VspI ATTAAT 1 cut(s) 452
XapI RAATTY 2 cut(s) 214, 660
XceI RCATGY 2 cut(s) 172, 671
ZrmI AGTACT 2 cut(s) 9, 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.