FvH4_1g18321

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001788
Physical Location & Seq
Reverse (-)
10663066 .. 10664828
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18321.t1

Sequence Viewer

Length: 1002 bp
ATGTTCTGCAGTATGAGGTTTCTCATGACATTGGTTCTCTTCTTTTTCTTTGCTAAATTTGATGCAATGAGATATGATCAACAAGATGATGTATCAGTAACGATCACCGTCAGTAAGAATGGTGGCGGAAAATTTAGCACTGTACAACAAGCCATTGATTCCATACCATCAAATAACGAAGGGTGGATTCGGATCCAGATTCAGCATGGTGTCTACCATGAAAAGGTTACAATCCCTCAGGACAAGCCATTTGTTATTCTTGAAGGAGACCCTAATCATCAAACAACGATCGAATTTGGAGCTTCTGGCAGTGTTGTTGAGAGTCCTACATTCAAGGCATTTGCAGATAATTTTGTGGCAAAAAATATAGTATTCAAGAATACATATGACCACCTTATCACATTTGATCCGAATAATGGGAGAAAAACTACTTGGGCGCCTGCTGCTAAAATTGCGGGAGACAAAGCAAGTTTCTATAACTGCACTTTCATCAGCTTGCAAGATACACTGACTGATTTCCAAGGTCGTCACTATTTCTATGGCTGCAATATTCAAGGTGCTATAGACTTCATATGGGGAAATGGCCAGTCAATTTACGAGAAATGTCGGATAATGGCTTTGAGCAAAATAATTGGCACTACTGGTTATATTACAGCTCAAGGTCGTGAAGGTCCAAATGAAACTACTGGGTTTGTCTTCAAGGATTGCCATTTATGTGGATCAGGACGTATACTTCTAGGTAGAGCATACAGAACATACGCAAGAGTATTGTTTTCGGGGACGTATATGGAGAACATTGTTGCACCTGAGGGTTGGTCAGAATGGGAAGTCCGACCAGTGGATACAATCACATTCTCAGAGGCGAAATGCAGAGGACCTGGAGCAAACATGTCTCATAGGATTTCCTGGGAGAAGAAGTTATCGGATGACGAAGTTGGATATTTAACAAATACTTCTTCTTTTATAAACCAAGATGGATGGTTGGAAAAGCAACCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

334

Amino Acids

37.51

Weight (kDa)

6.25

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 35 - 311 2.8e-60 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 965
AccB1I GGYRCC 1 cut(s) 436
AccI GTMKAC 2 cut(s) 213, 730
AciI CCGC 2 cut(s) 126, 455
AclWI GGATC 4 cut(s) 187, 200, 401, 727
AcoI YGGCCR 1 cut(s) 583
AcsI RAATTY 3 cut(s) 56, 131, 293
AcyI GRCGYC 1 cut(s) 437
AfaI GTAC 1 cut(s) 144
AfiI CCNNNNNNNGG 3 cut(s) 223, 416, 838
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 5 cut(s) 263, 334, 376, 554, 700
AjnI CCWGG 2 cut(s) 877, 905
AjuI GAANNNNNNNTTGG 2 cut(s) 415, 447
AluBI AGCT 3 cut(s) 302, 495, 656
AluI AGCT 3 cut(s) 302, 495, 656
Alw26I GTCTC 3 cut(s) 261, 453, 897
AlwI GGATC 4 cut(s) 187, 200, 401, 727
AoxI GGCC 1 cut(s) 583
ApeKI GCWGC 2 cut(s) 443, 543
ApoI RAATTY 3 cut(s) 56, 131, 293
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 2 cut(s) 671, 875
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 2 cut(s) 671, 875
AxyI CCTNAGG 2 cut(s) 237, 807
BalI TGGCCA 1 cut(s) 585
BamHI GGATCC 1 cut(s) 192
BanI GGYRCC 1 cut(s) 436
BbsI GAAGAC 1 cut(s) 688
BbvI GCAGC 2 cut(s) 430, 530
BccI CCATC 3 cut(s) 175, 968, 972
BciT130I CCWGG 2 cut(s) 879, 907
BciVI GTATCC 1 cut(s) 835
BclI TGATCA 1 cut(s) 76
BcoDI GTCTC 3 cut(s) 261, 453, 897
BfaI CTAG 1 cut(s) 737
BfmI CTRYAG 2 cut(s) 7, 561
BfoI RGCGCY 1 cut(s) 440
BfuI GTATCC 1 cut(s) 835
BisI GCNGC 2 cut(s) 444, 544
BlsI GCNGC 2 cut(s) 445, 545
Bme1390I CCNGG 2 cut(s) 879, 907
Bme18I GGWCC 2 cut(s) 671, 875
BmgT120I GGNCC 2 cut(s) 671, 875
BmiI GGNNCC 2 cut(s) 194, 438
BmrFI CCNGG 2 cut(s) 879, 907
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 1 cut(s) 688
BpmI CTGGAG 1 cut(s) 900
BpuEI CTTGAG 1 cut(s) 642
BsaBI GATNNNNATC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 520, 906
BsaXI ACNNNNNCTCC 2 cut(s) 902, 932
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 416, 838
Bse1I ACTGG 4 cut(s) 586, 646, 691, 836
Bse21I CCTNAGG 2 cut(s) 237, 807
Bse3DI GCAATG 1 cut(s) 72
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 2 cut(s) 879, 907
BseDI CCNNGG 2 cut(s) 520, 906
BseGI GGATG 2 cut(s) 931, 983
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 3 cut(s) 223, 416, 838
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 3 cut(s) 251, 798, 870
BseNI ACTGG 4 cut(s) 586, 646, 691, 836
BseXI GCAGC 2 cut(s) 430, 530
BsgI GTGCAG 1 cut(s) 466
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 1 cut(s) 585
BshNI GGYRCC 1 cut(s) 436
BsiEI CGRYCG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 3 cut(s) 223, 416, 838
BsmAI GTCTC 3 cut(s) 261, 453, 897
BsmFI GGGAC 1 cut(s) 793
BsnI GGCC 1 cut(s) 585
Bso31I GGTCTC 1 cut(s) 261
Bsp1407I TGTACA 1 cut(s) 142
Bsp143I GATC 6 cut(s) 76, 102, 192, 288, 406, 719
BspACI CCGC 2 cut(s) 126, 455
BspANI GGCC 1 cut(s) 585
BspCNI CTCAG 3 cut(s) 250, 799, 869
BspHI TCATGA 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 194, 438
BspMAI CTGCAG 1 cut(s) 11
BspPI GGATC 4 cut(s) 187, 200, 401, 727
BspT107I GGYRCC 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 72
BsrGI TGTACA 1 cut(s) 142
BsrI ACTGG 4 cut(s) 586, 646, 691, 836
BssECI CCNNGG 2 cut(s) 520, 906
BssMI GATC 6 cut(s) 76, 102, 192, 288, 406, 719
BssNAI GTATAC 1 cut(s) 731
BssNI GRCGYC 1 cut(s) 437
BssT1I CCWWGG 1 cut(s) 520
Bst1107I GTATAC 1 cut(s) 731
Bst2UI CCWGG 2 cut(s) 879, 907
Bst4CI ACNGT 2 cut(s) 109, 142
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 441, 497
BstDEI CTNAG 3 cut(s) 237, 807, 856
BstF5I GGATG 2 cut(s) 931, 983
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 6 cut(s) 79, 105, 195, 291, 409, 722
BstMAI GTCTC 3 cut(s) 261, 453, 897
BstMBI GATC 6 cut(s) 76, 102, 192, 288, 406, 719
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 2 cut(s) 443, 452
BstNI CCWGG 2 cut(s) 879, 907
BstNSI RCATGY 1 cut(s) 892
BstSCI CCNGG 2 cut(s) 877, 905
BstSFI CTRYAG 2 cut(s) 7, 561
BstV1I GCAGC 2 cut(s) 430, 530
BstV2I GAAGAC 1 cut(s) 688
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 716
BstYI RGATCY 1 cut(s) 192
BstZ17I GTATAC 1 cut(s) 731
Bsu36I CCTNAGG 2 cut(s) 237, 807
BsuI GTATCC 1 cut(s) 835
BsuRI GGCC 1 cut(s) 585
BtsCI GGATG 2 cut(s) 931, 983
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 4 cut(s) 138, 316, 506, 843
Cac8I GCNNGC 2 cut(s) 441, 497
CciI TCATGA 1 cut(s) 24
CfoI GCGC 1 cut(s) 439
Cfr13I GGNCC 2 cut(s) 671, 875
Csp6I GTAC 1 cut(s) 143
CviAII CATG 4 cut(s) 25, 206, 218, 889
CviJI RGCY 8 cut(s) 152, 247, 302, 495, 543, 585, 617, 656
CviKI_1 RGCY 8 cut(s) 152, 247, 302, 495, 543, 585, 617, 656
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 3 cut(s) 237, 807, 856
DinI GGCGCC 1 cut(s) 438
DpnI GATC 6 cut(s) 78, 104, 194, 290, 408, 721
DpnII GATC 6 cut(s) 76, 102, 192, 288, 406, 719
EaeI YGGCCR 1 cut(s) 583
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 520
Eco31I GGTCTC 1 cut(s) 261
Eco47I GGWCC 2 cut(s) 671, 875
Eco81I CCTNAGG 2 cut(s) 237, 807
EcoO109I RGGNCCY 1 cut(s) 875
EcoRII CCWGG 2 cut(s) 877, 905
EcoT14I CCWWGG 1 cut(s) 520
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 4 cut(s) 28, 209, 221, 892
FaqI GGGAC 1 cut(s) 793
FatI CATG 4 cut(s) 24, 205, 217, 888
FauI CCCGC 1 cut(s) 448
FauNDI CATATG 2 cut(s) 385, 572
FbaI TGATCA 1 cut(s) 76
FblI GTMKAC 2 cut(s) 213, 730
Fnu4HI GCNGC 2 cut(s) 444, 544
FokI GGATG 2 cut(s) 938, 990
Fsp4HI GCNGC 2 cut(s) 444, 544
FspBI CTAG 1 cut(s) 737
GlaI GCGC 1 cut(s) 438
GluI GCNGC 2 cut(s) 444, 544
GsuI CTGGAG 1 cut(s) 900
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 1 cut(s) 585
HhaI GCGC 1 cut(s) 439
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 4 cut(s) 28, 209, 221, 892
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HinfI GANTC 4 cut(s) 158, 187, 199, 322
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 214, 731
Hpy188I TCNGA 7 cut(s) 192, 411, 609, 820, 833, 859, 925
Hpy188III TCNNGA 7 cut(s) 25, 196, 239, 260, 376, 665, 723
Hpy8I GTNNAC 2 cut(s) 214, 731
HpyAV CCTTC 3 cut(s) 173, 257, 662
HpyCH4III ACNGT 2 cut(s) 109, 142
HpyCH4IV ACGT 2 cut(s) 727, 782
HpyCH4V TGCA 8 cut(s) 9, 65, 344, 483, 499, 546, 803, 870
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 452
HpyF3I CTNAG 3 cut(s) 237, 807, 856
HpySE526I ACGT 2 cut(s) 727, 782
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 4 cut(s) 28, 209, 221, 892
HspAI GCGC 1 cut(s) 437
KasI GGCGCC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 6 cut(s) 76, 102, 192, 288, 406, 719
LmnI GCTCC 2 cut(s) 299, 881
Lsp1109I GCAGC 2 cut(s) 430, 530
LweI GCATC 1 cut(s) 52
MaeI CTAG 1 cut(s) 737
MaeII ACGT 2 cut(s) 727, 782
MaeIII GTNAC 3 cut(s) 97, 226, 527
MalI GATC 6 cut(s) 78, 104, 194, 290, 408, 721
MboI GATC 6 cut(s) 76, 102, 192, 288, 406, 719
MboII GAAGA 4 cut(s) 31, 688, 925, 948
MflI RGATCY 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 7 cut(s) 56, 131, 293, 349, 450, 591, 630
MluNI TGGCCA 1 cut(s) 585
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 1 cut(s) 331
MmeI TCCRAC 4 cut(s) 587, 856, 916, 963
MnlI CCTC 5 cut(s) 9, 246, 802, 853, 866
Mox20I TGGCCA 1 cut(s) 585
MscI TGGCCA 1 cut(s) 585
MseI TTAA 2 cut(s) 944, 1000
MslI CAYNNNNRTG 1 cut(s) 714
Msp20I TGGCCA 1 cut(s) 585
MspR9I CCNGG 2 cut(s) 879, 907
MvaI CCWGG 2 cut(s) 879, 907
MwoI GCNNNNNNNGC 2 cut(s) 443, 452
NarI GGCGCC 1 cut(s) 437
NdeI CATATG 2 cut(s) 385, 572
NdeII GATC 6 cut(s) 76, 102, 192, 288, 406, 719
NlaIII CATG 4 cut(s) 28, 209, 221, 892
NlaIV GGNNCC 2 cut(s) 194, 438
NmuCI GTSAC 1 cut(s) 527
NspI RCATGY 1 cut(s) 892
PagI TCATGA 1 cut(s) 24
PciI ACATGT 1 cut(s) 888
PfeI GAWTC 3 cut(s) 158, 187, 199
PkrI GCNGC 2 cut(s) 445, 545
Ple19I CGATCG 1 cut(s) 291
PleI GAGTC 1 cut(s) 330
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 330
PpuMI RGGWCCY 1 cut(s) 875
PscI ACATGT 1 cut(s) 888
PsiI TTATAA 1 cut(s) 965
Psp5II RGGWCCY 1 cut(s) 875
Psp6I CCWGG 2 cut(s) 877, 905
PspGI CCWGG 2 cut(s) 877, 905
PspN4I GGNNCC 2 cut(s) 194, 438
PspPI GGNCC 2 cut(s) 671, 875
PspPPI RGGWCCY 1 cut(s) 875
PstI CTGCAG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 192
PvuI CGATCG 1 cut(s) 291
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 2 cut(s) 944, 1000
SatI GCNGC 2 cut(s) 444, 544
Sau3AI GATC 6 cut(s) 76, 102, 192, 288, 406, 719
Sau96I GGNCC 2 cut(s) 671, 875
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 2 cut(s) 879, 907
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 2 cut(s) 7, 561
SfoI GGCGCC 1 cut(s) 438
SinI GGWCC 2 cut(s) 671, 875
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse9I AATT 7 cut(s) 56, 131, 293, 349, 450, 591, 630
SsiI CCGC 2 cut(s) 126, 455
SspDI GGCGCC 1 cut(s) 436
SspI AATATT 1 cut(s) 550
SspMI CTAG 1 cut(s) 737
StyD4I CCNGG 2 cut(s) 877, 905
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 2 cut(s) 109, 142
TaiI ACGT 2 cut(s) 730, 785
TaqI TCGA 1 cut(s) 291
TasI AATT 7 cut(s) 56, 131, 293, 349, 450, 591, 630
TatI WGTACW 1 cut(s) 142
TfiI GAWTC 3 cut(s) 158, 187, 199
Tru1I TTAA 2 cut(s) 944, 1000
Tru9I TTAA 2 cut(s) 944, 1000
TscAI CASTG 4 cut(s) 145, 316, 513, 843
TseFI GTSAC 1 cut(s) 527
TseI GCWGC 2 cut(s) 443, 543
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 4 cut(s) 234, 478, 559, 693
TspRI CASTG 4 cut(s) 145, 316, 513, 843
VpaK11BI GGWCC 2 cut(s) 671, 875
XapI RAATTY 3 cut(s) 56, 131, 293
XceI RCATGY 1 cut(s) 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmiI GTMKAC 2 cut(s) 213, 730
XspI CTAG 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.