Rh3BG081100

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001788
Physical Location & Seq
Reverse (-)
6348199 .. 6349716
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG081100.1

Sequence Viewer

Length: 993 bp
ATGAAGTCTCTTATTCTTATTATTTCTGTGATATTGGCTTTTTTCTCTTTTCTGAATGGATTGGATGCATTCCGTCCACTAGACCAAGTTTCAACTACCATCGTAGTTGATCAGAATGGTAAAGGACAATTTACCACTGTTCAACAAGCCGTCGACTCTATACCACCAAATAACCCGTGGACTCTAATTCACCTCAACTCCGGTGTCTACAATGAAAAAGTTGAGATCAAGAAGCCATATATTATTCTTGAAGGTGACCGCGATCAGCAAACTGTGATTCAATATGGAGATGGCGGAAATGTTGTTACCAGTCCAACATTTAAGTTGAATGCGGATAATTTTGTGGCACGATATATTGTATTCAAGAATACCTATGATAATCTTCCTTCTAAATTAGACGCCAGCGGGGTCAACACTACTTGGGCACCTGCAGCTGCAATTAACGGAGACAGAGCAAGTTTTTACCATTGCAGCTTCAGTAGCTTGCAGGACACATTGACTGATGCCAAAGGTCGTCATTACTTCTATGACTGCAAAATTTCCGGCGCCATAGACTTCATTTGGGGAAATGCACAATCAATTTACGAGAATTGCCAGATCAATTCATTGGCATCAAAAATAGCTCCACGGACTGGTTTTATTACAGCACAGGCTCGTGAAAGTCCAGAGGAAAATACTGGTTTTGTATTCAAAAATTGTCATGTGACTGGATCTGGCCCTATATATCTAGGAAGAGCTCATACAAACCACGCAAGAGTATTGTTTGCTGGGACAACAATGGACAATGTTATTAGGGCAGAAGGTTGGGACGGGGCACGGTTTAAGGGCTCCGAGAATTTAATCTCCTTCTCGGAGGCCAATTGCAGGGGACCTGGAGCAACCATGTCGAAACGTGTAAAGTGGGAGAAGAAACTATCAGATCAAGAAGTAAATGATTTGACTAGTTTATCTTACATAAATCAAGATGGGTGGATAGAGAAGCAACCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

330

Amino Acids

36.59

Weight (kDa)

6.6

Isoelectric Point (pI)

23.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 34 - 321 9.1e-56 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 436
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 2 cut(s) 424, 545
AccB7I CCANNNNNTGG 1 cut(s) 632
AccI GTMKAC 2 cut(s) 153, 207
AccII CGCG 1 cut(s) 261
AciI CCGC 4 cut(s) 259, 294, 332, 405
AclWI GGATC 1 cut(s) 718
AcsI RAATTY 2 cut(s) 537, 835
AcuI CTGAAG 1 cut(s) 460
AcyI GRCGYC 2 cut(s) 399, 546
AfiI CCNNNNNNNGG 2 cut(s) 632, 864
AflIII ACRYGT 1 cut(s) 892
AgsI TTSAA 7 cut(s) 93, 143, 251, 281, 328, 364, 691
AhlI ACTAGT 1 cut(s) 941
AjnI CCWGG 1 cut(s) 871
AluBI AGCT 5 cut(s) 434, 474, 483, 623, 737
AluI AGCT 5 cut(s) 434, 474, 483, 623, 737
Alw21I GWGCWC 1 cut(s) 739
Alw26I GTCTC 2 cut(s) 12, 441
AlwI GGATC 1 cut(s) 718
AoxI GGCC 2 cut(s) 715, 855
ApeKI GCWGC 3 cut(s) 431, 434, 471
ApoI RAATTY 2 cut(s) 537, 835
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 2 cut(s) 716, 869
AsuHPI GGTGA 2 cut(s) 182, 266
AvaII GGWCC 1 cut(s) 869
BaeGI GKGCMC 2 cut(s) 427, 817
BanI GGYRCC 2 cut(s) 424, 545
BanII GRGCYC 2 cut(s) 739, 830
BarI GAAGNNNNNNTAC 2 cut(s) 724, 756
BauI CACGAG 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 739
BbvI GCAGC 3 cut(s) 421, 443, 483
BccI CCATC 3 cut(s) 107, 284, 959
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 867, 901
BciT130I CCWGG 1 cut(s) 873
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 12, 441
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 4 cut(s) 80, 728, 942, 987
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 3 cut(s) 432, 435, 472
BlsI GCNGC 3 cut(s) 433, 436, 473
Bme1390I CCNGG 1 cut(s) 873
Bme18I GGWCC 1 cut(s) 869
BmgT120I GGNCC 2 cut(s) 716, 869
BmiI GGNNCC 4 cut(s) 426, 547, 829, 870
BmrFI CCNGG 1 cut(s) 873
BmsI GCATC 3 cut(s) 55, 493, 620
BpmI CTGGAG 1 cut(s) 894
BsaHI GRCGYC 2 cut(s) 399, 546
BsaJI CCNNGG 2 cut(s) 176, 626
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 2 cut(s) 632, 864
Bse1I ACTGG 4 cut(s) 309, 637, 682, 712
Bse3DI GCAATG 1 cut(s) 466
BseBI CCWGG 1 cut(s) 873
BseDI CCNNGG 2 cut(s) 176, 626
BseGI GGATG 1 cut(s) 70
BseLI CCNNNNNNNGG 2 cut(s) 632, 864
BseMI GCAATG 1 cut(s) 466
BseNI ACTGG 4 cut(s) 309, 637, 682, 712
BseSI GKGCMC 2 cut(s) 427, 817
BseXI GCAGC 3 cut(s) 421, 443, 483
BseYI CCCAGC 1 cut(s) 767
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 2 cut(s) 717, 857
BshNI GGYRCC 2 cut(s) 424, 545
BsiHKAI GWGCWC 1 cut(s) 739
BsiSI CCGG 2 cut(s) 201, 543
BslFI GGGAC 3 cut(s) 784, 821, 882
BslI CCNNNNNNNGG 2 cut(s) 632, 864
BsmAI GTCTC 2 cut(s) 12, 441
BsmFI GGGAC 3 cut(s) 784, 821, 882
BsmI GAATGC 2 cut(s) 68, 334
BsnI GGCC 2 cut(s) 717, 857
Bsp1286I GDGCHC 4 cut(s) 427, 739, 817, 830
Bsp143I GATC 6 cut(s) 109, 225, 262, 597, 710, 919
BspACI CCGC 4 cut(s) 259, 294, 332, 405
BspANI GGCC 2 cut(s) 717, 857
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 4 cut(s) 426, 547, 829, 870
BspMAI CTGCAG 1 cut(s) 433
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 1 cut(s) 718
BspQI GCTCTTC 1 cut(s) 727
BspT107I GGYRCC 2 cut(s) 424, 545
BsrDI GCAATG 1 cut(s) 466
BsrI ACTGG 4 cut(s) 309, 637, 682, 712
BssECI CCNNGG 2 cut(s) 176, 626
BssMI GATC 6 cut(s) 109, 225, 262, 597, 710, 919
BssNI GRCGYC 2 cut(s) 399, 546
BssSI CACGAG 1 cut(s) 654
Bst2BI CACGAG 1 cut(s) 654
Bst2UI CCWGG 1 cut(s) 873
Bst4CI ACNGT 3 cut(s) 139, 274, 819
Bst6I CTCTTC 1 cut(s) 727
BstACI GRCGYC 2 cut(s) 399, 546
BstC8I GCNNGC 2 cut(s) 403, 485
BstDSI CCRYGG 2 cut(s) 176, 626
BstEII GGTNACC 1 cut(s) 254
BstF5I GGATG 1 cut(s) 70
BstFNI CGCG 1 cut(s) 261
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 6 cut(s) 112, 228, 265, 600, 713, 922
BstMAI GTCTC 2 cut(s) 12, 441
BstMBI GATC 6 cut(s) 109, 225, 262, 597, 710, 919
BstMWI GCNNNNNNNGC 2 cut(s) 431, 480
BstNI CCWGG 1 cut(s) 873
BstPI GGTNACC 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 871
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 2 cut(s) 427, 817
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 421, 443, 483
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
BsuRI GGCC 2 cut(s) 717, 857
BtgI CCRYGG 2 cut(s) 176, 626
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 135
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 2 cut(s) 403, 485
CfoI GCGC 1 cut(s) 548
Cfr13I GGNCC 2 cut(s) 716, 869
CseI GACGC 1 cut(s) 407
CspCI CAANNNNNGTGG 2 cut(s) 950, 985
CviAII CATG 2 cut(s) 701, 883
DinI GGCGCC 1 cut(s) 547
DpnI GATC 6 cut(s) 111, 227, 264, 599, 712, 921
DpnII GATC 6 cut(s) 109, 225, 262, 597, 710, 919
Eam1104I CTCTTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 727
EciI GGCGGA 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 737
Eco24I GRGCYC 2 cut(s) 739, 830
Eco47I GGWCC 1 cut(s) 869
Eco53kI GAGCTC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 460
Eco91I GGTNACC 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 737
EcoO109I RGGNCCY 1 cut(s) 869
EcoO65I GGTNACC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 871
EcoT22I ATGCAT 1 cut(s) 70
EcoT38I GRGCYC 2 cut(s) 739, 830
EgeI GGCGCC 1 cut(s) 547
EheI GGCGCC 1 cut(s) 547
FaeI CATG 2 cut(s) 704, 886
FaqI GGGAC 3 cut(s) 784, 821, 882
FatI CATG 2 cut(s) 700, 882
FauI CCCGC 1 cut(s) 398
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 2 cut(s) 153, 207
Fnu4HI GCNGC 3 cut(s) 432, 435, 472
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 2 cut(s) 739, 830
Fsp4HI GCNGC 3 cut(s) 432, 435, 472
FspBI CTAG 4 cut(s) 80, 728, 942, 987
GlaI GCGC 1 cut(s) 547
GluI GCNGC 3 cut(s) 432, 435, 472
GsaI CCCAGC 1 cut(s) 771
GsuI CTGGAG 1 cut(s) 894
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 2 cut(s) 717, 857
HapII CCGG 2 cut(s) 201, 543
HgaI GACGC 1 cut(s) 407
HhaI GCGC 1 cut(s) 548
Hin1I GRCGYC 2 cut(s) 399, 546
Hin1II CATG 2 cut(s) 704, 886
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HincII GTYRAC 2 cut(s) 154, 412
HindII GTYRAC 2 cut(s) 154, 412
HinfI GANTC 3 cut(s) 155, 181, 277
HpaII CCGG 2 cut(s) 201, 543
HphI GGTGA 2 cut(s) 182, 266
Hpy166II GTNNAC 5 cut(s) 77, 154, 180, 208, 412
Hpy188I TCNGA 5 cut(s) 54, 114, 832, 853, 919
Hpy188III TCNNGA 7 cut(s) 229, 248, 364, 656, 665, 923, 962
Hpy8I GTNNAC 5 cut(s) 77, 154, 180, 208, 412
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 4 cut(s) 245, 396, 794, 856
HpyCH4III ACNGT 3 cut(s) 139, 274, 819
HpyCH4IV ACGT 1 cut(s) 892
HpyCH4V TGCA 8 cut(s) 68, 431, 437, 471, 487, 534, 572, 864
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 480
HpySE526I ACGT 1 cut(s) 892
Hsp92I GRCGYC 2 cut(s) 399, 546
Hsp92II CATG 2 cut(s) 704, 886
HspAI GCGC 1 cut(s) 546
KasI GGCGCC 1 cut(s) 545
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 6 cut(s) 109, 225, 262, 597, 710, 919
LguI GCTCTTC 1 cut(s) 727
LmnI GCTCC 3 cut(s) 628, 833, 875
Lsp1109I GCAGC 3 cut(s) 421, 443, 483
LweI GCATC 3 cut(s) 55, 493, 620
MaeI CTAG 4 cut(s) 80, 728, 942, 987
MaeII ACGT 1 cut(s) 892
MaeIII GTNAC 3 cut(s) 254, 304, 703
MalI GATC 6 cut(s) 111, 227, 264, 599, 712, 921
MboI GATC 6 cut(s) 109, 225, 262, 597, 710, 919
MboII GAAGA 3 cut(s) 374, 744, 919
MfeI CAATTG 1 cut(s) 859
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 4 cut(s) 427, 739, 817, 830
Mly113I GGCGCC 1 cut(s) 546
MlyI GAGTC 2 cut(s) 149, 175
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 3 cut(s) 203, 661, 847
Mph1103I ATGCAT 1 cut(s) 70
MseI TTAA 4 cut(s) 321, 441, 822, 839
MspA1I CMGCKG 2 cut(s) 405, 434
MspI CCGG 2 cut(s) 201, 543
MspR9I CCNGG 1 cut(s) 873
MunI CAATTG 1 cut(s) 859
Mva1269I GAATGC 2 cut(s) 68, 334
MvaI CCWGG 1 cut(s) 873
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 431, 480
NarI GGCGCC 1 cut(s) 546
NdeII GATC 6 cut(s) 109, 225, 262, 597, 710, 919
NlaIII CATG 2 cut(s) 704, 886
NlaIV GGNNCC 4 cut(s) 426, 547, 829, 870
NmuCI GTSAC 2 cut(s) 254, 703
NsiI ATGCAT 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 436
PciSI GCTCTTC 1 cut(s) 727
PctI GAATGC 2 cut(s) 68, 334
PfeI GAWTC 1 cut(s) 277
PflMI CCANNNNNTGG 1 cut(s) 632
PkrI GCNGC 3 cut(s) 433, 436, 473
PleI GAGTC 2 cut(s) 149, 175
PluTI GGCGCC 1 cut(s) 549
PpsI GAGTC 2 cut(s) 149, 175
PpuMI RGGWCCY 1 cut(s) 869
Psp124BI GAGCTC 1 cut(s) 739
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 1 cut(s) 871
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 767
PspGI CCWGG 1 cut(s) 871
PspN4I GGNNCC 4 cut(s) 426, 547, 829, 870
PspPI GGNCC 2 cut(s) 716, 869
PspPPI RGGWCCY 1 cut(s) 869
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 710
PvuII CAGCTG 1 cut(s) 434
SacI GAGCTC 1 cut(s) 739
SalI GTCGAC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 727
SaqAI TTAA 4 cut(s) 321, 441, 822, 839
SatI GCNGC 3 cut(s) 432, 435, 472
Sau3AI GATC 6 cut(s) 109, 225, 262, 597, 710, 919
Sau96I GGNCC 2 cut(s) 716, 869
SchI GAGTC 2 cut(s) 149, 175
ScrFI CCNGG 1 cut(s) 873
SduI GDGCHC 4 cut(s) 427, 739, 817, 830
SfaNI GCATC 3 cut(s) 55, 493, 620
SfcI CTRYAG 1 cut(s) 429
SfoI GGCGCC 1 cut(s) 547
SinI GGWCC 1 cut(s) 869
SpeI ACTAGT 1 cut(s) 941
SsiI CCGC 4 cut(s) 259, 294, 332, 405
SspDI GGCGCC 1 cut(s) 545
SspMI CTAG 4 cut(s) 80, 728, 942, 987
SstI GAGCTC 1 cut(s) 739
StyD4I CCNGG 1 cut(s) 871
TaaI ACNGT 3 cut(s) 139, 274, 819
TaiI ACGT 1 cut(s) 895
TaqI TCGA 2 cut(s) 153, 887
TfiI GAWTC 1 cut(s) 277
Tru1I TTAA 4 cut(s) 321, 441, 822, 839
Tru9I TTAA 4 cut(s) 321, 441, 822, 839
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 2 cut(s) 254, 703
TseI GCWGC 3 cut(s) 431, 434, 471
Tsp45I GTSAC 2 cut(s) 254, 703
TspDTI ATGAA 4 cut(s) 17, 228, 547, 594
TspGWI ACGGA 3 cut(s) 62, 459, 643
TspRI CASTG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 632
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 2 cut(s) 537, 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XmiI GTMKAC 2 cut(s) 153, 207
XspI CTAG 4 cut(s) 80, 728, 942, 987
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.