MD02G1190500.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001788
Physical Location & Seq
Reverse (-)
17556746 .. 17560838
4093 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1190500.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGGAGTCTCTTATATCAACAATATTTTCCCTCCTCCTTTTCTTCGGTTCAACATTGGGGGGACGGTACAAAGCAACGAAAAACGCTTTGGAAAACGATGTTTCAAGTACCATCATAGTCGATAAGAGTGGTCGAGGAAATTTTACCACAGTGCAACAAGCCATCGATTCTGTACCACCAAATAACTCTCTGTGGATTCGTATCCTCCTTAACCCTGATGTGTATACGGAAAAAGTTATGATTCCAAAGGAGAAACCATACATCGTTCTTGAAGGAGACCCTAAATTTCCCGCTACAATCGAATACGGAGATGCTGGCAGTGTCATTGACAGTCCTACATTCAAGTTGTTTGCAGATAATTTTGTGGCGCGAAGTATAATATTCAAGAATTCATATGACCATCTTATCTTGCCGGATCCGAATGGAGGCAAAACTACTTGGGCGCCTGCAATCTTAATCTCTGCAGACAAAGCAAGTTTTCACCACTGCAGTTTTATAAGCTTGCAAGATACATTGACTGATGATGGTGGCCGTCACTATTTTTACGATTGCTTTATTGAAGGGGCTATAGACTTCATTTGGGGCAATGGCCAGTCAATTTATGAGAAATGCCAGATATTTTCGATAACCGATCGAATAGGGATTACTGGTTTTATTACAGCACAAGGCCGTAAAGCCCCAAACGAAACAACTGGTTTTGTGCTCAAAGATTGCTATGTAAACGGAACAGGTACTACATTTTTAGGAAGACCGTGGAGACCTTATTCGAGAGTATTGTTTGCCTCCACCTTCATGGAAAATATCATCACCCCTGAGGGTTGGTCCCCATGGCCGCCTGCTCCTGTGGATTCGGTTGCATTCTCGGAGGCAAATTGTCAAGGACCTGGAGCCAATTTGTCGGGTCGTGTCCCATGGGAGAAGAAGCTATCAGATGAAGAAGTGGCATATTTCACAAATCCGGCTTCTTTCATAGACCAAGAAGGATGGTTGAGAGGTCAACCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

336

Amino Acids

37.1

Weight (kDa)

5.02

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 38 - 325 8.9e-60 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 442
AccI GTMKAC 1 cut(s) 224
AccII CGCG 1 cut(s) 370
AciI CCGC 2 cut(s) 291, 833
AclWI GGATC 2 cut(s) 410, 423
AcoI YGGCCR 3 cut(s) 529, 589, 830
AcsI RAATTY 3 cut(s) 139, 284, 388
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 4 cut(s) 68, 109, 174, 733
AfiI CCNNNNNNNGG 1 cut(s) 425
AgsI TTSAA 6 cut(s) 51, 105, 272, 343, 385, 560
AjnI CCWGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 2 cut(s) 501, 925
AluI AGCT 2 cut(s) 501, 925
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 3 cut(s) 12, 270, 751
AlwI GGATC 2 cut(s) 410, 423
AoxI GGCC 4 cut(s) 529, 589, 667, 830
ApoI RAATTY 3 cut(s) 139, 284, 388
AspLEI GCGC 2 cut(s) 370, 445
AspS9I GGNCC 2 cut(s) 822, 881
AsuHPI GGTGA 2 cut(s) 473, 799
AvaII GGWCC 2 cut(s) 822, 881
AxyI CCTNAGG 1 cut(s) 813
BalI TGGCCA 1 cut(s) 591
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 442
BbsI GAAGAC 1 cut(s) 754
Bbv12I GWGCWC 1 cut(s) 705
BccI CCATC 5 cut(s) 119, 170, 408, 518, 978
BceAI ACGGC 2 cut(s) 516, 654
BciT130I CCWGG 1 cut(s) 885
BciVI GTATCC 1 cut(s) 212
BcoDI GTCTC 3 cut(s) 12, 270, 751
BfmI CTRYAG 3 cut(s) 462, 487, 567
BfoI RGCGCY 1 cut(s) 446
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 1 cut(s) 833
BlsI GCNGC 1 cut(s) 834
Bme1390I CCNGG 1 cut(s) 885
Bme18I GGWCC 2 cut(s) 822, 881
BmgT120I GGNCC 2 cut(s) 822, 881
BmiI GGNNCC 4 cut(s) 417, 444, 824, 889
BmrFI CCNGG 1 cut(s) 885
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 754
BpmI CTGGAG 1 cut(s) 906
Bsa29I ATCGAT 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 443
BsaI GGTCTC 2 cut(s) 270, 751
BsaJI CCNNGG 3 cut(s) 752, 827, 911
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse1I ACTGG 3 cut(s) 592, 652, 697
Bse21I CCTNAGG 1 cut(s) 813
Bse3DI GCAATG 1 cut(s) 592
Bse8I GATNNNNATC 1 cut(s) 200
BseBI CCWGG 1 cut(s) 885
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 752, 827, 911
BseGI GGATG 1 cut(s) 989
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 3 cut(s) 592, 652, 697
BseRI GAGGAG 1 cut(s) 23
Bsh1236I CGCG 1 cut(s) 370
Bsh1285I CGRYCG 1 cut(s) 634
BshFI GGCC 4 cut(s) 531, 591, 669, 832
BshNI GGYRCC 1 cut(s) 442
BshVI ATCGAT 1 cut(s) 165
BsiEI CGRYCG 1 cut(s) 634
BsiHKAI GWGCWC 1 cut(s) 705
BsiSI CCGG 2 cut(s) 413, 959
BslFI GGGAC 3 cut(s) 75, 808, 893
BslI CCNNNNNNNGG 1 cut(s) 425
BsmAI GTCTC 3 cut(s) 12, 270, 751
BsmFI GGGAC 3 cut(s) 75, 808, 893
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 4 cut(s) 531, 591, 669, 832
Bso31I GGTCTC 2 cut(s) 270, 751
Bsp1286I GDGCHC 1 cut(s) 705
Bsp143I GATC 2 cut(s) 415, 631
Bsp19I CCATGG 2 cut(s) 827, 911
BspACI CCGC 2 cut(s) 291, 833
BspANI GGCC 4 cut(s) 531, 591, 669, 832
BspCNI CTCAG 1 cut(s) 805
BspDI ATCGAT 1 cut(s) 165
BspFNI CGCG 1 cut(s) 370
BspLI GGNNCC 4 cut(s) 417, 444, 824, 889
BspMAI CTGCAG 2 cut(s) 466, 491
BspPI GGATC 2 cut(s) 410, 423
BspT107I GGYRCC 1 cut(s) 442
BspTNI GGTCTC 2 cut(s) 270, 751
BsrDI GCAATG 1 cut(s) 592
BsrI ACTGG 3 cut(s) 592, 652, 697
BssECI CCNNGG 3 cut(s) 752, 827, 911
BssMI GATC 2 cut(s) 415, 631
BssNAI GTATAC 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 443
BssT1I CCWWGG 2 cut(s) 827, 911
Bst1107I GTATAC 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 4 cut(s) 66, 151, 332, 753
BstACI GRCGYC 1 cut(s) 443
BstC8I GCNNGC 4 cut(s) 316, 447, 503, 837
BstDEI CTNAG 1 cut(s) 813
BstDSI CCRYGG 3 cut(s) 752, 827, 911
BstF5I GGATG 1 cut(s) 989
BstFNI CGCG 1 cut(s) 370
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 2 cut(s) 370, 445
BstKTI GATC 2 cut(s) 418, 634
BstMAI GTCTC 3 cut(s) 12, 270, 751
BstMBI GATC 2 cut(s) 415, 631
BstMCI CGRYCG 1 cut(s) 634
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstNI CCWGG 1 cut(s) 885
BstSCI CCNGG 1 cut(s) 883
BstSFI CTRYAG 3 cut(s) 462, 487, 567
BstUI CGCG 1 cut(s) 370
BstV2I GAAGAC 1 cut(s) 754
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 793
BstYI RGATCY 1 cut(s) 415
BstZ17I GTATAC 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 165
Bsu36I CCTNAGG 1 cut(s) 813
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 4 cut(s) 531, 591, 669, 832
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 3 cut(s) 752, 827, 911
BtsCI GGATG 1 cut(s) 989
BtsI GCAGTG 2 cut(s) 325, 484
BtsIMutI CAGTG 3 cut(s) 156, 325, 484
Cac8I GCNNGC 4 cut(s) 316, 447, 503, 837
CfoI GCGC 2 cut(s) 370, 445
Cfr13I GGNCC 2 cut(s) 822, 881
ClaI ATCGAT 1 cut(s) 165
Csp6I GTAC 4 cut(s) 67, 108, 173, 732
CviAII CATG 3 cut(s) 793, 828, 912
CviQI GTAC 4 cut(s) 67, 108, 173, 732
DdeI CTNAG 1 cut(s) 813
DinI GGCGCC 1 cut(s) 444
DpnI GATC 2 cut(s) 417, 633
DpnII GATC 2 cut(s) 415, 631
EaeI YGGCCR 3 cut(s) 529, 589, 830
Eco130I CCWWGG 2 cut(s) 827, 911
Eco31I GGTCTC 2 cut(s) 270, 751
Eco47I GGWCC 2 cut(s) 822, 881
Eco81I CCTNAGG 1 cut(s) 813
EcoO109I RGGNCCY 1 cut(s) 881
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 2 cut(s) 827, 911
EgeI GGCGCC 1 cut(s) 444
EheI GGCGCC 1 cut(s) 444
ErhI CCWWGG 2 cut(s) 827, 911
FaeI CATG 3 cut(s) 796, 831, 915
FaqI GGGAC 3 cut(s) 75, 808, 893
FatI CATG 3 cut(s) 792, 827, 911
FauI CCCGC 1 cut(s) 298
FauNDI CATATG 1 cut(s) 394
FblI GTMKAC 1 cut(s) 224
Fnu4HI GCNGC 1 cut(s) 833
FokI GGATG 1 cut(s) 996
Fsp4HI GCNGC 1 cut(s) 833
GlaI GCGC 2 cut(s) 369, 444
GluI GCNGC 1 cut(s) 833
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 4 cut(s) 531, 591, 669, 832
HapII CCGG 2 cut(s) 413, 959
HhaI GCGC 2 cut(s) 370, 445
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 3 cut(s) 796, 831, 915
Hin6I GCGC 2 cut(s) 368, 443
HinP1I GCGC 2 cut(s) 368, 443
HincII GTYRAC 1 cut(s) 998
HindII GTYRAC 1 cut(s) 998
HindIII AAGCTT 1 cut(s) 499
HinfI GANTC 5 cut(s) 5, 167, 196, 241, 848
HpaII CCGG 2 cut(s) 413, 959
HphI GGTGA 2 cut(s) 473, 799
Hpy166II GTNNAC 3 cut(s) 225, 721, 998
Hpy188I TCNGA 3 cut(s) 420, 865, 931
Hpy188III TCNNGA 3 cut(s) 269, 385, 768
Hpy8I GTNNAC 3 cut(s) 225, 721, 998
HpyAV CCTTC 4 cut(s) 266, 554, 799, 974
HpyCH4III ACNGT 4 cut(s) 66, 151, 332, 753
HpyCH4V TGCA 7 cut(s) 154, 353, 449, 464, 489, 505, 857
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 1 cut(s) 813
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 3 cut(s) 796, 831, 915
HspAI GCGC 2 cut(s) 368, 443
KasI GGCGCC 1 cut(s) 442
Kzo9I GATC 2 cut(s) 415, 631
LmnI GCTCC 2 cut(s) 844, 887
LweI GCATC 1 cut(s) 301
MaeIII GTNAC 1 cut(s) 533
MalI GATC 2 cut(s) 417, 633
MboI GATC 2 cut(s) 415, 631
MboII GAAGA 4 cut(s) 34, 759, 931, 947
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 705
MlsI TGGCCA 1 cut(s) 591
MluCI AATT 8 cut(s) 139, 284, 358, 388, 597, 871, 892, 1003
MluNI TGGCCA 1 cut(s) 591
Mly113I GGCGCC 1 cut(s) 443
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 9 cut(s) 41, 44, 128, 215, 419, 793, 808, 859, 986
Mox20I TGGCCA 1 cut(s) 591
MscI TGGCCA 1 cut(s) 591
MseI TTAA 3 cut(s) 210, 455, 1006
MslI CAYNNNNRTG 1 cut(s) 791
Msp20I TGGCCA 1 cut(s) 591
MspI CCGG 2 cut(s) 413, 959
MspR9I CCNGG 1 cut(s) 885
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 1 cut(s) 470
NarI GGCGCC 1 cut(s) 443
NcoI CCATGG 2 cut(s) 827, 911
NdeI CATATG 1 cut(s) 394
NdeII GATC 2 cut(s) 415, 631
NlaIII CATG 3 cut(s) 796, 831, 915
NlaIV GGNNCC 4 cut(s) 417, 444, 824, 889
NmuCI GTSAC 1 cut(s) 533
PctI GAATGC 1 cut(s) 857
PfeI GAWTC 4 cut(s) 167, 196, 241, 848
PkrI GCNGC 1 cut(s) 834
Ple19I CGATCG 1 cut(s) 634
PleI GAGTC 1 cut(s) 13
PluTI GGCGCC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 881
PsiI TTATAA 1 cut(s) 497
Psp5II RGGWCCY 1 cut(s) 881
Psp6I CCWGG 1 cut(s) 883
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 4 cut(s) 417, 444, 824, 889
PspPI GGNCC 2 cut(s) 822, 881
PspPPI RGGWCCY 1 cut(s) 881
PsrI GAACNNNNNNTAC 2 cut(s) 718, 750
PstI CTGCAG 2 cut(s) 466, 491
PsuI RGATCY 1 cut(s) 415
PvuI CGATCG 1 cut(s) 634
RsaI GTAC 4 cut(s) 68, 109, 174, 733
RsaNI GTAC 4 cut(s) 67, 108, 173, 732
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 3 cut(s) 210, 455, 1006
SatI GCNGC 1 cut(s) 833
Sau3AI GATC 2 cut(s) 415, 631
Sau96I GGNCC 2 cut(s) 822, 881
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 885
SduI GDGCHC 1 cut(s) 705
SetI ASST 7 cut(s) 503, 733, 763, 791, 886, 927, 997
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 3 cut(s) 462, 487, 567
SfoI GGCGCC 1 cut(s) 444
SinI GGWCC 2 cut(s) 822, 881
SmiMI CAYNNNNRTG 1 cut(s) 791
Sse9I AATT 8 cut(s) 139, 284, 358, 388, 597, 871, 892, 1003
SsiI CCGC 2 cut(s) 291, 833
SspDI GGCGCC 1 cut(s) 442
SspI AATATT 2 cut(s) 24, 381
StyD4I CCNGG 1 cut(s) 883
StyI CCWWGG 2 cut(s) 827, 911
TaaI ACNGT 4 cut(s) 66, 151, 332, 753
TaqI TCGA 7 cut(s) 120, 133, 165, 300, 623, 634, 767
TasI AATT 8 cut(s) 139, 284, 358, 388, 597, 871, 892, 1003
TauI GCSGC 1 cut(s) 835
TfiI GAWTC 4 cut(s) 167, 196, 241, 848
Tru1I TTAA 3 cut(s) 210, 455, 1006
Tru9I TTAA 3 cut(s) 210, 455, 1006
TscAI CASTG 3 cut(s) 156, 325, 491
TseFI GTSAC 1 cut(s) 533
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 5 cut(s) 381, 565, 781, 948, 958
TspGWI ACGGA 3 cut(s) 242, 321, 738
TspRI CASTG 3 cut(s) 156, 325, 491
VpaK11BI GGWCC 2 cut(s) 822, 881
XapI RAATTY 3 cut(s) 139, 284, 388
XmiI GTMKAC 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.