AT5G26810

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001788
Physical Location & Seq
Forward (+)
9430849 .. 9433000
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G26810.1

Sequence Viewer

Length: 1020 bp
ATGCCTTGTTTGTTCATCTTCATAGCTTTGCTTCTAAGTTCTTGTATTGGAACGTTGAAAGCCCTTGATCAGACTTGTGGCAACAAAGTCGTGAACACAATCGTCGTCGACCAAGCCGGTTCAGGTAAGTTCCGTACTGTTCAAGCTGCCATTGATTCGGTTGGTGAATTGAATTCTCTATGGATCAAAATCAAAGTAAAACGAGGTGTTTACGTAGAGAAGGTGATAATACCATATAATAAACCGTGCATAATAGTAGAAGGAGAGGGACAAAGAGTCACCACCATCACGTATAATGGTCATGCAGCCACTGACGTCAGCTCAACTTTCACTTCATATCCATCCCATATTGTCGTCCGAAACTTATCAATCATGAACACTTATAATCGTTTGACTAGTCTAACTAAAGCAAACGGTATGAGTTGGGATATAAAGCCAGCGGTTGCGATTTCGGTTTATGGCGATAAATCAGCGTTTTACAACTGTGATTTCTTGGGGTTGCAAGATACAGTGTGGGATAATCTTGGCAGACATCACTTCAAAAATTGTTACATCGAAGGTGCTATCGATTTCATTTTTGGAAGTGGCCAATCTGTTTACGAGGACTGTCATATAAACGCAACGGCAGGAGCATTGGCATCAAAAGTGTCGTTTGGGTACATAACGGCTCAAGGAAGAAGTAGCGATTCGGACCCAAGTGGGTTCGTGTTCCTACGAGGTAGTGTTTCAGGGAGCACCAGTGTCTATCTTGGACGAGCCTATGGTCCATTCAGTCGAGTGATATTCATCCAAACCGATCTCTCTTCCGTCGTGCACCCTGAGGGTTGGTACTCTTGGCACTATGGTGGATACGAGATGAGTTTCACGTATGCAGAAGTTGAGTGTAAAGGAGCAGGATCGGATATGTCACGAAGAGTTCCTTGGATTGATAAACTTCATTCTTTTTACACTAAACAACAATTCTCTATCTCTAATTTCATTGACCAAGATCAATGGATCTCTAATATTCCTCGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

339

Amino Acids

37.55

Weight (kDa)

7.58

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 34 - 329 1.2e-58 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AatII GACGTC 1 cut(s) 318
AccI GTMKAC 1 cut(s) 108
AciI CCGC 1 cut(s) 440
AclI AACGTT 1 cut(s) 53
AclWI GGATC 3 cut(s) 191, 904, 1004
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 1 cut(s) 172
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 3 cut(s) 136, 659, 830
AgsI TTSAA 4 cut(s) 58, 143, 172, 541
AhdI GACNNNNNGTC 1 cut(s) 275
AhlI ACTAGT 1 cut(s) 395
AjuI GAANNNNNNNTTGG 2 cut(s) 904, 936
AleI CACNNNNGTG 1 cut(s) 843
AluBI AGCT 3 cut(s) 26, 146, 321
AluI AGCT 3 cut(s) 26, 146, 321
Alw21I GWGCWC 2 cut(s) 737, 816
Alw44I GTGCAC 1 cut(s) 812
AlwI GGATC 3 cut(s) 191, 904, 1004
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 1 cut(s) 586
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 2 cut(s) 146, 305
ApoI RAATTY 1 cut(s) 172
AspS9I GGNCC 2 cut(s) 691, 764
AsuHPI GGTGA 3 cut(s) 176, 235, 271
AvaII GGWCC 2 cut(s) 691, 764
AxyI CCTNAGG 1 cut(s) 819
BaeGI GKGCMC 1 cut(s) 816
BalI TGGCCA 1 cut(s) 588
Bbv12I GWGCWC 2 cut(s) 737, 816
BbvI GCAGC 2 cut(s) 133, 317
BccI CCATC 2 cut(s) 293, 349
BceAI ACGGC 2 cut(s) 639, 681
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciVI GTATCC 1 cut(s) 842
BclI TGATCA 1 cut(s) 67
BcuI ACTAGT 1 cut(s) 395
BfaI CTAG 1 cut(s) 396
BfuI GTATCC 1 cut(s) 842
BisI GCNGC 2 cut(s) 147, 306
BlsI GCNGC 2 cut(s) 148, 307
Bme18I GGWCC 2 cut(s) 691, 764
BmeRI GACNNNNNGTC 1 cut(s) 275
BmgT120I GGNCC 2 cut(s) 691, 764
BmiI GGNNCC 1 cut(s) 693
BmsI GCATC 1 cut(s) 647
BpuEI CTTGAG 1 cut(s) 654
Bsa29I ATCGAT 1 cut(s) 567
BsaAI YACGTR 3 cut(s) 214, 291, 867
BsaBI GATNNNNATC 2 cut(s) 188, 785
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 920
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 738
Bse21I CCTNAGG 1 cut(s) 819
Bse8I GATNNNNATC 2 cut(s) 188, 785
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 1 cut(s) 920
BseGI GGATG 2 cut(s) 341, 786
BseJI GATNNNNATC 2 cut(s) 188, 785
BseMII CTCAG 1 cut(s) 810
BseNI ACTGG 1 cut(s) 738
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 2 cut(s) 133, 317
BshFI GGCC 1 cut(s) 588
BshVI ATCGAT 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 737, 816
BsiSI CCGG 1 cut(s) 117
BslFI GGGAC 1 cut(s) 282
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 1 cut(s) 588
Bsp1286I GDGCHC 2 cut(s) 737, 816
Bsp143I GATC 6 cut(s) 67, 183, 796, 896, 988, 996
BspACI CCGC 1 cut(s) 440
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 1 cut(s) 811
BspDI ATCGAT 1 cut(s) 567
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 1 cut(s) 693
BspPI GGATC 3 cut(s) 191, 904, 1004
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 738
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 920
BssMI GATC 6 cut(s) 67, 183, 796, 896, 988, 996
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 920
Bst4CI ACNGT 6 cut(s) 139, 246, 416, 485, 511, 608
Bst6I CTCTTC 2 cut(s) 808, 907
BstACI GRCGYC 1 cut(s) 315
BstBAI YACGTR 3 cut(s) 214, 291, 867
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 2 cut(s) 35, 819
BstF5I GGATG 2 cut(s) 341, 786
BstKTI GATC 6 cut(s) 70, 186, 799, 899, 991, 999
BstMBI GATC 6 cut(s) 67, 183, 796, 896, 988, 996
BstSLI GKGCMC 1 cut(s) 816
BstSNI TACGTA 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 133, 317
BstX2I RGATCY 1 cut(s) 996
BstYI RGATCY 1 cut(s) 996
Bsu15I ATCGAT 1 cut(s) 567
Bsu36I CCTNAGG 1 cut(s) 819
BsuI GTATCC 1 cut(s) 842
BsuRI GGCC 1 cut(s) 588
BsuTUI ATCGAT 1 cut(s) 567
BtsCI GGATG 2 cut(s) 341, 786
BtsIMutI CAGTG 3 cut(s) 309, 516, 745
Cac8I GCNNGC 1 cut(s) 438
CaiI CAGNNNCTG 1 cut(s) 311
CciI TCATGA 1 cut(s) 372
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 691, 764
ClaI ATCGAT 1 cut(s) 567
Csp6I GTAC 3 cut(s) 135, 658, 829
CviAII CATG 2 cut(s) 302, 373
CviQI GTAC 3 cut(s) 135, 658, 829
DdeI CTNAG 2 cut(s) 35, 819
DpnI GATC 6 cut(s) 69, 185, 798, 898, 990, 998
DpnII GATC 6 cut(s) 67, 183, 796, 896, 988, 996
DriI GACNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 808, 907
Eam1105I GACNNNNNGTC 1 cut(s) 275
EarI CTCTTC 2 cut(s) 808, 907
Eco105I TACGTA 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 920
Eco47I GGWCC 2 cut(s) 691, 764
Eco81I CCTNAGG 1 cut(s) 819
EcoRI GAATTC 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 920
ErhI CCWWGG 1 cut(s) 920
FaeI CATG 2 cut(s) 305, 376
FalI AAGNNNNNCTT 2 cut(s) 904, 936
FaqI GGGAC 1 cut(s) 282
FatI CATG 2 cut(s) 301, 372
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 2 cut(s) 147, 306
FokI GGATG 2 cut(s) 328, 773
Fsp4HI GCNGC 2 cut(s) 147, 306
FspBI CTAG 1 cut(s) 396
GluI GCNGC 2 cut(s) 147, 306
HaeIII GGCC 1 cut(s) 588
HapII CCGG 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 2 cut(s) 305, 376
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 3 cut(s) 155, 276, 686
HpaII CCGG 1 cut(s) 117
HphI GGTGA 3 cut(s) 176, 235, 271
Hpy166II GTNNAC 5 cut(s) 94, 109, 211, 598, 814
Hpy188I TCNGA 4 cut(s) 72, 359, 691, 901
Hpy188III TCNNGA 3 cut(s) 91, 373, 909
Hpy8I GTNNAC 5 cut(s) 94, 109, 211, 598, 814
Hpy99I CGWCG 3 cut(s) 107, 110, 812
HpyAV CCTTC 3 cut(s) 214, 254, 551
HpyCH4III ACNGT 6 cut(s) 139, 246, 416, 485, 511, 608
HpyCH4IV ACGT 5 cut(s) 53, 213, 290, 315, 866
HpyCH4V TGCA 5 cut(s) 249, 305, 502, 814, 872
HpyF3I CTNAG 2 cut(s) 35, 819
HpySE526I ACGT 5 cut(s) 53, 213, 290, 315, 866
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 2 cut(s) 305, 376
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 6 cut(s) 67, 183, 796, 896, 988, 996
LmnI GCTCC 3 cut(s) 629, 732, 890
LpnPI CCDG 8 cut(s) 108, 130, 450, 612, 714, 751, 831, 879
Lsp1109I GCAGC 2 cut(s) 133, 317
LweI GCATC 1 cut(s) 647
MaeI CTAG 1 cut(s) 396
MaeII ACGT 5 cut(s) 53, 213, 290, 315, 866
MaeIII GTNAC 3 cut(s) 277, 548, 906
MalI GATC 6 cut(s) 69, 185, 798, 898, 990, 998
MboI GATC 6 cut(s) 67, 183, 796, 896, 988, 996
MboII GAAGA 4 cut(s) 10, 687, 795, 924
MflI RGATCY 1 cut(s) 996
MhlI GDGCHC 2 cut(s) 737, 816
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 5 cut(s) 167, 172, 544, 959, 973
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 1 cut(s) 285
MnlI CCTC 6 cut(s) 197, 259, 595, 710, 814, 1020
Mox20I TGGCCA 1 cut(s) 588
MscI TGGCCA 1 cut(s) 588
MseI TTAA 1 cut(s) 1018
MslI CAYNNNNRTG 1 cut(s) 843
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 1 cut(s) 117
NdeII GATC 6 cut(s) 67, 183, 796, 896, 988, 996
NlaIII CATG 2 cut(s) 305, 376
NlaIV GGNNCC 1 cut(s) 693
NmuCI GTSAC 2 cut(s) 277, 906
OliI CACNNNNGTG 1 cut(s) 843
PagI TCATGA 1 cut(s) 372
PfeI GAWTC 2 cut(s) 155, 686
PkrI GCNGC 2 cut(s) 148, 307
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Ppu21I YACGTR 3 cut(s) 214, 291, 867
PsiI TTATAA 1 cut(s) 384
Psp1406I AACGTT 1 cut(s) 53
PspN4I GGNNCC 1 cut(s) 693
PspPI GGNCC 2 cut(s) 691, 764
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 1 cut(s) 996
RsaI GTAC 3 cut(s) 136, 659, 830
RsaNI GTAC 3 cut(s) 135, 658, 829
RseI CAYNNNNRTG 1 cut(s) 843
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 1018
SatI GCNGC 2 cut(s) 147, 306
Sau3AI GATC 6 cut(s) 67, 183, 796, 896, 988, 996
Sau96I GGNCC 2 cut(s) 691, 764
SchI GAGTC 1 cut(s) 285
SduI GDGCHC 2 cut(s) 737, 816
SfaNI GCATC 1 cut(s) 647
SinI GGWCC 2 cut(s) 691, 764
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 1 cut(s) 669
SmoI CTYRAG 1 cut(s) 669
SnaBI TACGTA 1 cut(s) 214
SpeI ACTAGT 1 cut(s) 395
Sse9I AATT 5 cut(s) 167, 172, 544, 959, 973
SsiI CCGC 1 cut(s) 440
SspI AATATT 1 cut(s) 1006
SspMI CTAG 1 cut(s) 396
StyI CCWWGG 1 cut(s) 920
TaaI ACNGT 6 cut(s) 139, 246, 416, 485, 511, 608
TaiI ACGT 5 cut(s) 56, 216, 293, 318, 869
TaqI TCGA 4 cut(s) 108, 555, 567, 775
TasI AATT 5 cut(s) 167, 172, 544, 959, 973
TfiI GAWTC 2 cut(s) 155, 686
Tru1I TTAA 1 cut(s) 1018
Tru9I TTAA 1 cut(s) 1018
TscAI CASTG 3 cut(s) 316, 516, 745
TseFI GTSAC 2 cut(s) 277, 906
TseI GCWGC 2 cut(s) 146, 305
Tsp45I GTSAC 2 cut(s) 277, 906
TspDTI ATGAA 8 cut(s) 4, 10, 324, 389, 562, 775, 926, 967
TspGWI ACGGA 2 cut(s) 122, 796
TspRI CASTG 3 cut(s) 316, 516, 745
VneI GTGCAC 1 cut(s) 812
VpaK11BI GGWCC 2 cut(s) 691, 764
XapI RAATTY 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 1 cut(s) 396
ZraI GACGTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.