AT3G02570

mannose-6-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001016
Physical Location & Seq
Reverse (-)
543346 .. 545602
2257 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02570.1

Sequence Viewer

Length: 1299 bp
ATGGAGATCGCTACTGTCGTAAAGGCTAATGGCGGTTGTGAAGCGGATCGACGACGGCTTCGCCGATTGAGATGCTCTGTTAAGGATTACGATTGGGGCAAAATTGGATCGGATTCACTCGTTTACAGGGTTTATGCGGCGAATTCAGATTACGAGATCGATCCTACACGACCTTACGCCGAGCTATGGATGGGGACTCACGAGTCTGGTCCGAGTTACTTGGAGGATGCTGATGGATCCAATGGCGTGACCCTTAGATCCTGGATCACCGAAAACCCTAAATCTCTTGGAAATAGGGTTTTGGAGAAATGGGGTTGCGATCTCCCCTTTCTGTTCAAGGTGTTGTCGGTGGCAAGGCCGTTGTCGATTCAAGCACATCCAGATAAGAAATTAGCAAAGAAAATGCACAAAGCTCATCCTAATCTTTATAAAGACGACAATCACAAACCAGAGATGGCTTTGGCCTATACTCAATTCGAGGCTCTCTGTGGCTTTATACCTCTTCAGGAGCTTAAGAGTGTCATTCGAGCTATTCCGGAGATTGAAGAACTTGTTGGGAGTGAAGAAGCAAACCAAGTTTTCTGTATCACTGAACATGATGAGGAAAAAGTGAAATCTGTTGTCCGAACAATCTTCACCCTGCTAATGTCTGCTGACGCCGATACAACAAAGAAAATTGTATCCAAATTAAAACGCCGACTGCATATGGAGAGTCAGGAACGCCAATTGACAGACAAGGAACGGCTGGTTTTGAAGCTAGAGAAGCAATATCCAAATGATATTGGAGTGATATCTGCATTCTTCTTCAACTATGTGAAGCTCAATCCTGGCGAAGCCTTGTATCTTGGTGCAAACGAGCCCCACGCATACTTATTCGGTGAGTGCCTTGAGGTCATGGCGACTTCAGACAATGTAGTACGAGCTGGCCTCACTTCCAAGCCTCTGGATATTCAAACACTTTGTTCAATGCTCTCTTATAAACTGGGATATCCGGAAATCCTGAAGGGAACAAGGATTCGACCATACATAACAAGATACCTGCCGCCTTTCGAAGAGTTTGAAGTTGATCTGTGTGATCTTCCCAGTGGAGCCTCGACGGTGTTTCCCTCTGTTCCAGGCCCTTCTCTTCTGCTTGTACTCCAAGGTGAAGGAAGAATGTCTACAGAAGCATCAGCCGATGGGATTTCAATGGGAGATGTTTTATTTGTTCCTGCAGATACCGAGATTCACTTGAGGTCTTCATCTGACTTGAAGTTGTACAGAGCAGGAATCAACAGTAGGTTTTTGTTTCCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

432

Amino Acids

48.57

Weight (kDa)

5.94

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 21 - 167 5.8e-48 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 184 - 268 2.3e-13 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 429, 978
AasI GACNNNNNNGTC 1 cut(s) 202
Acc36I ACCTGC 1 cut(s) 1047
AccI GTMKAC 1 cut(s) 1160
AccIII TCCGGA 2 cut(s) 535, 991
AciI CCGC 4 cut(s) 33, 44, 137, 1043
AclWI GGATC 7 cut(s) 54, 115, 155, 231, 244, 252, 272
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 3 cut(s) 488, 888, 1022
AcyI GRCGYC 1 cut(s) 657
AfaI GTAC 3 cut(s) 918, 1137, 1259
AfiI CCNNNNNNNGG 1 cut(s) 186
AflII CTTAAG 1 cut(s) 512
AjnI CCWGG 3 cut(s) 260, 826, 1114
AjuI GAANNNNNNNTTGG 2 cut(s) 537, 569
AluBI AGCT 7 cut(s) 184, 413, 511, 530, 757, 820, 923
AluI AGCT 7 cut(s) 184, 413, 511, 530, 757, 820, 923
AlwI GGATC 7 cut(s) 54, 115, 155, 231, 244, 252, 272
Aor13HI TCCGGA 2 cut(s) 535, 991
AoxI GGCC 4 cut(s) 356, 462, 925, 1117
ApoI RAATTY 1 cut(s) 142
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspS9I GGNCC 2 cut(s) 209, 1118
AsuHPI GGTGA 4 cut(s) 259, 628, 890, 1157
AsuII TTCGAA 1 cut(s) 1050
AvaII GGWCC 1 cut(s) 209
BamHI GGATCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 861
BarI GAAGNNNNNNTAC 2 cut(s) 1144, 1176
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 1230
BccI CCATC 4 cut(s) 184, 227, 448, 1172
BceAI ACGGC 3 cut(s) 71, 343, 758
BcgI CGANNNNNNTGC 2 cut(s) 54, 88
BciT130I CCWGG 3 cut(s) 262, 828, 1116
BciVI GTATCC 1 cut(s) 691
BfaI CTAG 1 cut(s) 758
BfmI CTRYAG 3 cut(s) 1161, 1212, 1295
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 1047
BfuI GTATCC 1 cut(s) 691
BisI GCNGC 2 cut(s) 138, 1043
BlsI GCNGC 2 cut(s) 139, 1044
Bme1390I CCNGG 3 cut(s) 262, 828, 1116
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 209, 1118
BmiI GGNNCC 2 cut(s) 238, 1090
BmrFI CCNGG 3 cut(s) 262, 828, 1116
BmrI ACTGGG 2 cut(s) 992, 1077
BmsI GCATC 3 cut(s) 62, 217, 1178
BmuI ACTGGG 2 cut(s) 992, 1077
BpiI GAAGAC 1 cut(s) 1230
BplI GAGNNNNNCTC 2 cut(s) 912, 944
Bpu14I TTCGAA 1 cut(s) 1050
BpuEI CTTGAG 2 cut(s) 908, 1252
Bsa29I ATCGAT 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 657
BsaJI CCNNGG 1 cut(s) 1141
BsaWI WCCGGW 2 cut(s) 535, 991
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 2 cut(s) 987, 1083
BseAI TCCGGA 2 cut(s) 535, 991
BseBI CCWGG 3 cut(s) 262, 828, 1116
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 1141
BseGI GGATG 4 cut(s) 195, 232, 376, 415
BseLI CCNNNNNNNGG 1 cut(s) 186
BseNI ACTGG 2 cut(s) 987, 1083
BshFI GGCC 4 cut(s) 358, 464, 927, 1119
BshVI ATCGAT 1 cut(s) 159
BsiSI CCGG 2 cut(s) 536, 992
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 4 cut(s) 358, 464, 927, 1119
Bsp119I TTCGAA 1 cut(s) 1050
Bsp1286I GDGCHC 1 cut(s) 861
Bsp13I TCCGGA 2 cut(s) 535, 991
Bsp1407I TGTACA 1 cut(s) 1257
BspACI CCGC 4 cut(s) 33, 44, 137, 1043
BspANI GGCC 4 cut(s) 358, 464, 927, 1119
BspDI ATCGAT 1 cut(s) 159
BspEI TCCGGA 2 cut(s) 535, 991
BspLI GGNNCC 2 cut(s) 238, 1090
BspMAI CTGCAG 1 cut(s) 1216
BspMI ACCTGC 1 cut(s) 1047
BspPI GGATC 7 cut(s) 54, 115, 155, 231, 244, 252, 272
BspT104I TTCGAA 1 cut(s) 1050
BspTI CTTAAG 1 cut(s) 512
BsrGI TGTACA 1 cut(s) 1257
BsrI ACTGG 2 cut(s) 987, 1083
BssECI CCNNGG 1 cut(s) 1141
BssNI GRCGYC 1 cut(s) 657
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 1141
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 3 cut(s) 262, 828, 1116
Bst4CI ACNGT 3 cut(s) 16, 1099, 1277
Bst6I CTCTTC 3 cut(s) 507, 1047, 1131
BstACI GRCGYC 1 cut(s) 657
BstAFI CTTAAG 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 1257
BstBI TTCGAA 1 cut(s) 1050
BstC8I GCNNGC 1 cut(s) 925
BstDEI CTNAG 1 cut(s) 254
BstF5I GGATG 4 cut(s) 195, 232, 376, 415
BstMWI GCNNNNNNNGC 1 cut(s) 763
BstNI CCWGG 3 cut(s) 262, 828, 1116
BstSCI CCNGG 3 cut(s) 260, 826, 1114
BstSFI CTRYAG 3 cut(s) 1161, 1212, 1295
BstV2I GAAGAC 1 cut(s) 1230
BstX2I RGATCY 2 cut(s) 236, 257
BstXI CCANNNNNNTGG 1 cut(s) 943
BstYI RGATCY 2 cut(s) 236, 257
Bsu15I ATCGAT 1 cut(s) 159
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 4 cut(s) 358, 464, 927, 1119
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 4 cut(s) 195, 232, 376, 415
BtsIMutI CAGTG 2 cut(s) 588, 1090
BveI ACCTGC 1 cut(s) 1047
Cac8I GCNNGC 1 cut(s) 925
Cfr13I GGNCC 2 cut(s) 209, 1118
ClaI ATCGAT 1 cut(s) 159
CseI GACGC 1 cut(s) 665
Csp6I GTAC 3 cut(s) 917, 1136, 1258
CviAII CATG 2 cut(s) 596, 895
CviQI GTAC 3 cut(s) 917, 1136, 1258
DdeI CTNAG 1 cut(s) 254
DrdI GACNNNNNNGTC 1 cut(s) 202
DseDI GACNNNNNNGTC 1 cut(s) 202
Eam1104I CTCTTC 3 cut(s) 507, 1047, 1131
EarI CTCTTC 3 cut(s) 507, 1047, 1131
Eco130I CCWWGG 1 cut(s) 1141
Eco24I GRGCYC 1 cut(s) 861
Eco32I GATATC 2 cut(s) 792, 989
Eco47I GGWCC 1 cut(s) 209
Eco57I CTGAAG 3 cut(s) 488, 888, 1022
EcoO109I RGGNCCY 1 cut(s) 1118
EcoRI GAATTC 1 cut(s) 142
EcoRII CCWGG 3 cut(s) 260, 826, 1114
EcoRV GATATC 2 cut(s) 792, 989
EcoT14I CCWWGG 1 cut(s) 1141
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 1141
FaeI CATG 2 cut(s) 599, 898
FaqI GGGAC 1 cut(s) 208
FatI CATG 2 cut(s) 595, 894
FauNDI CATATG 1 cut(s) 705
FblI GTMKAC 1 cut(s) 1160
Fnu4HI GCNGC 2 cut(s) 138, 1043
FokI GGATG 4 cut(s) 202, 239, 363, 402
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 2 cut(s) 138, 1043
FspBI CTAG 1 cut(s) 758
GluI GCNGC 2 cut(s) 138, 1043
HaeIII GGCC 4 cut(s) 358, 464, 927, 1119
HapII CCGG 2 cut(s) 536, 992
HgaI GACGC 1 cut(s) 665
Hin1I GRCGYC 1 cut(s) 657
Hin1II CATG 2 cut(s) 599, 898
HinfI GANTC 8 cut(s) 113, 196, 203, 367, 712, 1015, 1225, 1269
HpaII CCGG 2 cut(s) 536, 992
HphI GGTGA 4 cut(s) 259, 628, 890, 1157
Hpy166II GTNNAC 2 cut(s) 124, 1161
Hpy188I TCNGA 6 cut(s) 112, 148, 213, 626, 907, 1246
Hpy188III TCNNGA 8 cut(s) 200, 380, 506, 536, 716, 944, 992, 1000
Hpy8I GTNNAC 2 cut(s) 124, 1161
Hpy99I CGWCG 3 cut(s) 54, 57, 1099
HpyAV CCTTC 3 cut(s) 997, 1131, 1142
HpyCH4III ACNGT 3 cut(s) 16, 1099, 1277
HpyCH4V TGCA 5 cut(s) 406, 703, 797, 851, 1214
HpyF10VI GCNNNNNNNGC 1 cut(s) 763
HpyF3I CTNAG 1 cut(s) 254
Hsp92I GRCGYC 1 cut(s) 657
Hsp92II CATG 2 cut(s) 599, 898
Kpn2I TCCGGA 2 cut(s) 535, 991
LmnI GCTCC 2 cut(s) 508, 1088
LweI GCATC 3 cut(s) 62, 217, 1178
MaeI CTAG 1 cut(s) 758
MaeIII GTNAC 2 cut(s) 215, 247
MfeI CAATTG 1 cut(s) 725
MflI RGATCY 2 cut(s) 236, 257
MhlI GDGCHC 1 cut(s) 861
MluCI AATT 7 cut(s) 102, 142, 389, 473, 675, 686, 725
MlyI GAGTC 3 cut(s) 190, 212, 721
MroI TCCGGA 2 cut(s) 535, 991
MseI TTAA 3 cut(s) 81, 513, 689
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 2 cut(s) 536, 992
MspR9I CCNGG 3 cut(s) 262, 828, 1116
MunI CAATTG 1 cut(s) 725
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 3 cut(s) 262, 828, 1116
MwoI GCNNNNNNNGC 1 cut(s) 763
NdeI CATATG 1 cut(s) 705
NlaIII CATG 2 cut(s) 599, 898
NlaIV GGNNCC 2 cut(s) 238, 1090
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 247
NspV TTCGAA 1 cut(s) 1050
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 61
PctI GAATGC 1 cut(s) 797
PfeI GAWTC 5 cut(s) 113, 367, 1015, 1225, 1269
PfoI TCCNGGA 1 cut(s) 260
PkrI GCNGC 2 cut(s) 139, 1044
PleI GAGTC 3 cut(s) 190, 211, 720
PpsI GAGTC 3 cut(s) 190, 211, 720
PsiI TTATAA 2 cut(s) 429, 978
Psp6I CCWGG 3 cut(s) 260, 826, 1114
PspGI CCWGG 3 cut(s) 260, 826, 1114
PspN4I GGNNCC 2 cut(s) 238, 1090
PspPI GGNCC 2 cut(s) 209, 1118
PstI CTGCAG 1 cut(s) 1216
PsuI RGATCY 2 cut(s) 236, 257
RsaI GTAC 3 cut(s) 918, 1137, 1259
RsaNI GTAC 3 cut(s) 917, 1136, 1258
SaqAI TTAA 3 cut(s) 81, 513, 689
SatI GCNGC 2 cut(s) 138, 1043
Sau96I GGNCC 2 cut(s) 209, 1118
SchI GAGTC 3 cut(s) 190, 212, 721
ScrFI CCNGG 3 cut(s) 262, 828, 1116
SduI GDGCHC 1 cut(s) 861
SfaNI GCATC 3 cut(s) 62, 217, 1178
SfcI CTRYAG 3 cut(s) 1161, 1212, 1295
SfuI TTCGAA 1 cut(s) 1050
SinI GGWCC 1 cut(s) 209
SmlI CTYRAG 3 cut(s) 512, 887, 1231
SmoI CTYRAG 3 cut(s) 512, 887, 1231
Sse9I AATT 7 cut(s) 102, 142, 389, 473, 675, 686, 725
SsiI CCGC 4 cut(s) 33, 44, 137, 1043
SspMI CTAG 1 cut(s) 758
StyD4I CCNGG 3 cut(s) 260, 826, 1114
StyI CCWWGG 1 cut(s) 1141
TaaI ACNGT 3 cut(s) 16, 1099, 1277
TaqI TCGA 8 cut(s) 49, 159, 365, 477, 526, 1018, 1050, 1094
TasI AATT 7 cut(s) 102, 142, 389, 473, 675, 686, 725
TatI WGTACW 2 cut(s) 1135, 1257
TauI GCSGC 2 cut(s) 140, 1045
TfiI GAWTC 5 cut(s) 113, 367, 1015, 1225, 1269
Tru1I TTAA 3 cut(s) 81, 513, 689
Tru9I TTAA 3 cut(s) 81, 513, 689
TscAI CASTG 2 cut(s) 595, 1090
TseFI GTSAC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 1230
TspRI CASTG 2 cut(s) 595, 1090
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 1160
XspI CTAG 1 cut(s) 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.