Rmu_sc0003135.1_g000012

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003135.1
OG0001016
Physical Location & Seq
Forward (+)
50035 .. 51514
1480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003135.1_g000012.1.cds

Sequence Viewer

Length: 1302 bp
atgcaacaaccaaagcagaggcagaggagtgttcagaggctaagatgctctgtcatgaactacgactggggtaagaaaggccgagactctgtggtgggcaggttgtgtgcctccaattctcattctgaaatcgacctagaaaagccttacgctgaaatgtggatgggaacacatgactctggaccctcctttttgattgatcaggatctgaataataataatagcaccactactgggtcatctccgtctctcagagactgggtttctaacaaccctaatgacatgctcggtcacaaggttgtccaaaaatggggctcctctgatctgcctttcttgtttaaggtgcaatccgtggcaaaggcattgtcaatacaggcacacccagataaggaattggccaaggctttgcacaagtctatgccagatatttacaaggatgataattataagcctgagatggcgctggccttgacacattttcaggctctttgtggttttatcactcttgaggagcttaagaaggtgattggtaatgtccctgagattgtagaactggttggtagtgaggtcgaaaatcaagtgcttcgcacgaccaaagaagatggggaggataaagtcaagtctgttatacgtttgatttttacccaactaatgtcaactaacaaggaaattataaccacagtaacaactaaattgaagagtcgtttacagacggaaagccaggtgaggcagctgacagaaaaggagcagctagtgttgcaattggaaaagcaatatccgaacgatgtgggtgtgatatctgcattctttcttaaccatgtgaagcttaatcctggagaagcattatgccttggagcaaatgaaccccatgcctatatatctggggattgcatcgagtgcatggcaacctcagacaacgtagttagggctggccttactcccaagcatcgagatatcaaaaccctttgttctatgctcacatataagcagggtttccctgaaatcttgcgaggagttgctgtgaattctcatgtaacaaggtacataccgccttttgaagaatttgaggttgatcggatccatcttccccacggagaatcaacagagtttcctgcagtacctggtccttccatttttgtggtcacttttgggaagggaaccatacgtacgagtaatggagatcatgcaatcatccaaggagatgttctgtttgcacctgcacacactgagattagcataacaagtgcatctgagttgcacatatatagagccggagtgaatagcacattcttagcctcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

433

Amino Acids

48.22

Weight (kDa)

6.32

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 447, 674
AarI CACCTGC 1 cut(s) 1225
Acc36I ACCTGC 2 cut(s) 90, 1225
AciI CCGC 1 cut(s) 1049
AclWI GGATC 3 cut(s) 213, 1072, 1085
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 1024, 1061
AfaI GTAC 3 cut(s) 1043, 1119, 1168
AfiI CCNNNNNNNGG 3 cut(s) 234, 310, 388
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 2 cut(s) 697, 1058
AjnI CCWGG 3 cut(s) 720, 832, 1120
AjuI GAANNNNNNNTTGG 2 cut(s) 1188, 1220
AluBI AGCT 4 cut(s) 514, 733, 751, 826
AluI AGCT 4 cut(s) 514, 733, 751, 826
Alw26I GTCTC 3 cut(s) 78, 249, 252
AlwI GGATC 3 cut(s) 213, 1072, 1085
AlwNI CAGNNNCTG 3 cut(s) 208, 258, 1121
AoxI GGCC 4 cut(s) 79, 396, 465, 931
ApeKI GCWGC 2 cut(s) 730, 748
ApoI RAATTY 2 cut(s) 1024, 1061
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 2 cut(s) 182, 1124
AsuHPI GGTGA 2 cut(s) 535, 736
AvaII GGWCC 2 cut(s) 182, 1124
BalI TGGCCA 1 cut(s) 398
BamHI GGATCC 1 cut(s) 1077
BanII GRGCYC 1 cut(s) 317
BbvI GCAGC 2 cut(s) 742, 760
BccI CCATC 4 cut(s) 157, 451, 596, 1089
BciT130I CCWGG 3 cut(s) 722, 834, 1122
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 3 cut(s) 78, 249, 252
BfaI CTAG 2 cut(s) 137, 752
BfmI CTRYAG 1 cut(s) 1113
BfoI RGCGCY 1 cut(s) 464
BfrI CTTAAG 1 cut(s) 515
BfuAI ACCTGC 2 cut(s) 90, 1225
BisI GCNGC 2 cut(s) 731, 749
BlsI GCNGC 2 cut(s) 732, 750
Bme1390I CCNGG 3 cut(s) 722, 834, 1122
Bme18I GGWCC 2 cut(s) 182, 1124
BmgT120I GGNCC 2 cut(s) 182, 1124
BmiI GGNNCC 4 cut(s) 184, 316, 1079, 1159
BmrFI CCNGG 3 cut(s) 722, 834, 1122
BmrI ACTGGG 3 cut(s) 76, 243, 268
BmsI GCATC 4 cut(s) 35, 900, 955, 1256
BmuI ACTGGG 3 cut(s) 76, 243, 268
BpmI CTGGAG 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 527
BsaAI YACGTR 1 cut(s) 1166
BsaBI GATNNNNATC 1 cut(s) 204
BsaJI CCNNGG 5 cut(s) 351, 399, 850, 1090, 1195
BsaXI ACNNNNNCTCC 2 cut(s) 1005, 1035
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 310, 388
Bse1I ACTGG 4 cut(s) 71, 238, 263, 558
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 3 cut(s) 722, 834, 1122
BseDI CCNNGG 5 cut(s) 351, 399, 850, 1090, 1195
BseGI GGATG 3 cut(s) 168, 442, 1191
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 3 cut(s) 234, 310, 388
BseMII CTCAG 6 cut(s) 265, 444, 531, 924, 1218, 1242
BseNI ACTGG 4 cut(s) 71, 238, 263, 558
BseRI GAGGAG 4 cut(s) 40, 307, 524, 1026
BseXI GCAGC 2 cut(s) 742, 760
BsgI GTGCAG 1 cut(s) 1203
BshFI GGCC 4 cut(s) 81, 398, 467, 933
BsiSI CCGG 1 cut(s) 1272
BsiWI CGTACG 1 cut(s) 1166
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 3 cut(s) 234, 310, 388
BsmAI GTCTC 3 cut(s) 78, 249, 252
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 1 cut(s) 803
BsnI GGCC 4 cut(s) 81, 398, 467, 933
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
BspACI CCGC 1 cut(s) 1049
BspANI GGCC 4 cut(s) 81, 398, 467, 933
BspCNI CTCAG 6 cut(s) 264, 445, 532, 923, 1219, 1243
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 4 cut(s) 184, 316, 1079, 1159
BspMAI CTGCAG 1 cut(s) 1117
BspMI ACCTGC 2 cut(s) 90, 1225
BspPI GGATC 3 cut(s) 213, 1072, 1085
BspTI CTTAAG 1 cut(s) 515
BsrI ACTGG 4 cut(s) 71, 238, 263, 558
BssECI CCNNGG 5 cut(s) 351, 399, 850, 1090, 1195
BssMI GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
BssT1I CCWWGG 3 cut(s) 399, 850, 1195
Bst2UI CCWGG 3 cut(s) 722, 834, 1122
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 1 cut(s) 692
BstAFI CTTAAG 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 897
BstBAI YACGTR 1 cut(s) 1166
BstC8I GCNNGC 2 cut(s) 465, 931
BstDEI CTNAG 8 cut(s) 41, 251, 453, 540, 910, 1227, 1251, 1291
BstDSI CCRYGG 2 cut(s) 351, 1090
BstF5I GGATG 3 cut(s) 168, 442, 1191
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 6 cut(s) 202, 208, 325, 1075, 1080, 1183
BstMAI GTCTC 3 cut(s) 78, 249, 252
BstMBI GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
BstMWI GCNNNNNNNGC 2 cut(s) 757, 897
BstNI CCWGG 3 cut(s) 722, 834, 1122
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 3 cut(s) 720, 832, 1120
BstSFI CTRYAG 1 cut(s) 1113
BstSNI TACGTA 1 cut(s) 1166
BstV1I GCAGC 2 cut(s) 742, 760
BstX2I RGATCY 2 cut(s) 205, 1077
BstXI CCANNNNNNTGG 1 cut(s) 1138
BstYI RGATCY 2 cut(s) 205, 1077
BsuRI GGCC 4 cut(s) 81, 398, 467, 933
BtgI CCRYGG 2 cut(s) 351, 1090
BtsCI GGATG 3 cut(s) 168, 442, 1191
BtsIMutI CAGTG 1 cut(s) 1224
BveI ACCTGC 2 cut(s) 90, 1225
Cac8I GCNNGC 2 cut(s) 465, 931
CaiI CAGNNNCTG 3 cut(s) 208, 258, 1121
CciI TCATGA 1 cut(s) 54
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 2 cut(s) 182, 1124
CsiI ACCWGGT 1 cut(s) 1120
Csp6I GTAC 3 cut(s) 1042, 1118, 1167
CviAII CATG 8 cut(s) 55, 173, 283, 818, 869, 901, 1031, 1184
CviQI GTAC 3 cut(s) 1042, 1118, 1167
DdeI CTNAG 8 cut(s) 41, 251, 453, 540, 910, 1227, 1251, 1291
DpnI GATC 6 cut(s) 201, 207, 324, 1074, 1079, 1182
DpnII GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Eco105I TACGTA 1 cut(s) 1166
Eco130I CCWWGG 3 cut(s) 399, 850, 1195
Eco24I GRGCYC 1 cut(s) 317
Eco32I GATATC 2 cut(s) 798, 955
Eco47I GGWCC 2 cut(s) 182, 1124
EcoRI GAATTC 1 cut(s) 1024
EcoRII CCWGG 3 cut(s) 720, 832, 1120
EcoRV GATATC 2 cut(s) 798, 955
EcoT14I CCWWGG 3 cut(s) 399, 850, 1195
EcoT38I GRGCYC 1 cut(s) 317
ErhI CCWWGG 3 cut(s) 399, 850, 1195
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 8 cut(s) 58, 176, 286, 821, 872, 904, 1034, 1187
FaqI GGGAC 1 cut(s) 521
FatI CATG 8 cut(s) 54, 172, 282, 817, 868, 900, 1030, 1183
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 2 cut(s) 731, 749
FokI GGATG 3 cut(s) 175, 449, 1178
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 2 cut(s) 731, 749
FspBI CTAG 2 cut(s) 137, 752
GlaI GCGC 1 cut(s) 462
GluI GCNGC 2 cut(s) 731, 749
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 4 cut(s) 81, 398, 467, 933
HapII CCGG 1 cut(s) 1272
HhaI GCGC 1 cut(s) 463
Hin1II CATG 8 cut(s) 58, 176, 286, 821, 872, 904, 1034, 1187
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 4 cut(s) 86, 176, 700, 1097
HpaII CCGG 1 cut(s) 1272
HphI GGTGA 2 cut(s) 535, 736
Hpy166II GTNNAC 2 cut(s) 657, 707
Hpy188I TCNGA 9 cut(s) 36, 127, 210, 254, 322, 780, 913, 1077, 1252
Hpy188III TCNNGA 5 cut(s) 55, 180, 203, 506, 950
Hpy8I GTNNAC 2 cut(s) 657, 707
HpyAV CCTTC 3 cut(s) 514, 1137, 1147
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4IV ACGT 3 cut(s) 631, 918, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 757, 897
HpyF3I CTNAG 8 cut(s) 41, 251, 453, 540, 910, 1227, 1251, 1291
HpySE526I ACGT 3 cut(s) 631, 918, 1165
Hsp92II CATG 8 cut(s) 58, 176, 286, 821, 872, 904, 1034, 1187
HspAI GCGC 1 cut(s) 461
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
LmnI GCTCC 4 cut(s) 320, 511, 745, 854
Lsp1109I GCAGC 2 cut(s) 742, 760
LweI GCATC 4 cut(s) 35, 900, 955, 1256
MabI ACCWGGT 1 cut(s) 1120
MaeI CTAG 2 cut(s) 137, 752
MaeII ACGT 3 cut(s) 631, 918, 1165
MaeIII GTNAC 4 cut(s) 290, 682, 1033, 1141
MalI GATC 6 cut(s) 201, 207, 324, 1074, 1079, 1182
MboI GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
MboII GAAGA 4 cut(s) 611, 709, 1070, 1076
MfeI CAATTG 1 cut(s) 761
MflI RGATCY 2 cut(s) 205, 1077
MhlI GDGCHC 1 cut(s) 317
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 8 cut(s) 115, 392, 442, 669, 692, 761, 1024, 1061
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 3 cut(s) 80, 170, 709
Mox20I TGGCCA 1 cut(s) 398
MscI TGGCCA 1 cut(s) 398
MseI TTAA 4 cut(s) 339, 516, 813, 828
MslI CAYNNNNRTG 1 cut(s) 1136
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 733
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 1 cut(s) 1272
MspR9I CCNGG 3 cut(s) 722, 834, 1122
MunI CAATTG 1 cut(s) 761
Mva1269I GAATGC 1 cut(s) 803
MvaI CCWGG 3 cut(s) 722, 834, 1122
MwoI GCNNNNNNNGC 2 cut(s) 757, 897
NdeII GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
NlaIII CATG 8 cut(s) 58, 176, 286, 821, 872, 904, 1034, 1187
NlaIV GGNNCC 4 cut(s) 184, 316, 1079, 1159
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 2 cut(s) 290, 1141
NspI RCATGY 1 cut(s) 286
PagI TCATGA 1 cut(s) 54
PaqCI CACCTGC 1 cut(s) 1225
PctI GAATGC 1 cut(s) 803
PfeI GAWTC 1 cut(s) 1097
Pfl23II CGTACG 1 cut(s) 1166
PfoI TCCNGGA 1 cut(s) 832
PkrI GCNGC 2 cut(s) 732, 750
PleI GAGTC 3 cut(s) 80, 170, 708
PpsI GAGTC 3 cut(s) 80, 170, 708
Ppu21I YACGTR 1 cut(s) 1166
PsiI TTATAA 2 cut(s) 447, 674
Psp6I CCWGG 3 cut(s) 720, 832, 1120
PspGI CCWGG 3 cut(s) 720, 832, 1120
PspLI CGTACG 1 cut(s) 1166
PspN4I GGNNCC 4 cut(s) 184, 316, 1079, 1159
PspPI GGNCC 2 cut(s) 182, 1124
PsrI GAACNNNNNNTAC 2 cut(s) 1150, 1182
PstI CTGCAG 1 cut(s) 1117
PstNI CAGNNNCTG 3 cut(s) 208, 258, 1121
PsuI RGATCY 2 cut(s) 205, 1077
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 3 cut(s) 1043, 1119, 1168
RsaNI GTAC 3 cut(s) 1042, 1118, 1167
RseI CAYNNNNRTG 1 cut(s) 1136
SaqAI TTAA 4 cut(s) 339, 516, 813, 828
SatI GCNGC 2 cut(s) 731, 749
Sau3AI GATC 6 cut(s) 199, 205, 322, 1072, 1077, 1180
Sau96I GGNCC 2 cut(s) 182, 1124
SchI GAGTC 3 cut(s) 80, 170, 709
ScrFI CCNGG 3 cut(s) 722, 834, 1122
SduI GDGCHC 1 cut(s) 317
SexAI ACCWGGT 1 cut(s) 1120
SfaNI GCATC 4 cut(s) 35, 900, 955, 1256
SfcI CTRYAG 1 cut(s) 1113
SinI GGWCC 2 cut(s) 182, 1124
SmiMI CAYNNNNRTG 1 cut(s) 1136
SmlI CTYRAG 2 cut(s) 506, 515
SmoI CTYRAG 2 cut(s) 506, 515
SnaBI TACGTA 1 cut(s) 1166
Sse9I AATT 8 cut(s) 115, 392, 442, 669, 692, 761, 1024, 1061
SsiI CCGC 1 cut(s) 1049
SspMI CTAG 2 cut(s) 137, 752
StyD4I CCNGG 3 cut(s) 720, 832, 1120
StyI CCWWGG 3 cut(s) 399, 850, 1195
TaaI ACNGT 1 cut(s) 682
TaiI ACGT 3 cut(s) 634, 921, 1168
TaqI TCGA 4 cut(s) 132, 570, 894, 949
TaqII GACCGA 1 cut(s) 278
TasI AATT 8 cut(s) 115, 392, 442, 669, 692, 761, 1024, 1061
TfiI GAWTC 1 cut(s) 1097
Tru1I TTAA 4 cut(s) 339, 516, 813, 828
Tru9I TTAA 4 cut(s) 339, 516, 813, 828
TscAI CASTG 1 cut(s) 1231
TseFI GTSAC 2 cut(s) 290, 1141
TseI GCWGC 2 cut(s) 730, 748
Tsp45I GTSAC 2 cut(s) 290, 1141
TspDTI ATGAA 2 cut(s) 71, 876
TspGWI ACGGA 4 cut(s) 234, 340, 728, 1107
TspRI CASTG 1 cut(s) 1231
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 182, 1124
XapI RAATTY 2 cut(s) 1024, 1061
XceI RCATGY 1 cut(s) 286
XspI CTAG 2 cut(s) 137, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.