MD16G1040600.v1.1

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001016
Physical Location & Seq
Forward (+)
2883292 .. 2886393
3102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1040600.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGGGACAAGAACATGGAGCAAATGAGAAAATGGAGCAAATGGAGCCGCCGTGGGACAAGAACATGAAGAAGAAGCGCAAGCGGATCCACCGCCCCGTCAGGCTGAGATGCTCCGTCAAGAATTACGATTGGGGTATTTTCGGCCGCAATTCCAAGGTCGCCAGGCTTTTCGCGCTCAATTCCGGGTCCGACATCGACTCGGGCAGACCGTACGCCGAGTTCTGGATCGGGACTCATGAATCTGGGCCGTCCTATGCGGATTCCGGGTCGGAGCCCTTGAGTCTCAAGGCCTGGATCTCGCAGGATCCCAGTGTGCTTGGCGACAAGGTGGTTGACAAGTGGGGTGCTGATCTTCCGTTCTTGTTTAAGGTGCTTTCGGTTGGGAAGGCGTTGTCGATACAGGCTCATCCGGATAAAGAACTGGCTAGGGTGTTGCACAAGTCACACCCAAGTGTTTATAGAGATGACAATCACAAGCCTGAAATGGCATTGGCATTGACGGAGTTCGAGGCACTTTGTGGGTTTATCAGTGTCAAGGAGCTCAAGGATATGCTTAACAGTGTTCCCGAGATTGCAGAATTGGTTGTGGTTGCAGACGCAGAGCGAATTCTACTTGGTAGTGAGCATCATGAGAATTGGAATGCAAAAACAGATATAGAGTCCATCTTCACTCGACTTATGTTACTAAGTAAAGATACAATTGCTGACATGATTTCTAGACTGAAAAGACGTCTTAATTTAGAGAAAAAGAAAAGGCTTTTGACGGTTAAAGAACAACTGGTACTCAGGTTGGAGAGTCAATACCCAGATGACGTTGGTGCAATAGCAGCGTTTTTTCTTAACTATGTGAAGCTGAACCGTGGTGAAGCTATTAGTCTTGGGCCGAATGAACCTCATGCATATATTTCTGGTGAATGCATAGAGTGTATGGCAACTTCTGACAATGTTGTCCGTGCCGGCCTTACTTCCAAACCACTGGATGTTCAAACACTTCTTTCTATGCTCAAATACAGACAGGGCTCTCCAGAAATTATGCAAGGCGTTTCTTTGAATCCATACACAACGAGGTATCTCCCACCTTTTGAAGAATTTGAAGTCGACTGCTGTAACATTCCTCGCTCTGCATCGGTGGTTTTTCCTTCAGTCCTAGGACCATCTCTTTTCCTGTTTACAGCCGGGAAGGGACATTTTGATGCAGGTCTGTCCGAGGATGATATAGTGGTGGAGGGTGATGATTTAGTTGAAGAGGGTGAGGTTTTCTTTGTGCCTGCCAACACGAAGATTAGCATTACCGCCAAATCAACAGAACTACAATTGTACAGGGTGGGAGTGAACAGCAGATTTTTTAGGGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

453

Amino Acids

50.69

Weight (kDa)

5.95

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 34 - 175 9.2e-46 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 214 - 280 2.2e-08 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 947
AatII GACGTC 1 cut(s) 733
Acc36I ACCTGC 1 cut(s) 1187
AccI GTMKAC 1 cut(s) 1098
AccII CGCG 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 409
AciI CCGC 6 cut(s) 47, 82, 91, 145, 257, 1293
AclWI GGATC 6 cut(s) 79, 92, 233, 299, 302, 312
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 2 cut(s) 606, 1088
AcuI CTGAAG 1 cut(s) 1125
AcyI GRCGYC 1 cut(s) 730
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 3 cut(s) 212, 783, 1319
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 5 cut(s) 986, 1051, 1085, 1094, 1244
AjnI CCWGG 2 cut(s) 161, 290
AleI CACNNNNGTG 1 cut(s) 450
AluBI AGCT 3 cut(s) 541, 853, 869
AluI AGCT 3 cut(s) 541, 853, 869
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 6 cut(s) 79, 92, 233, 299, 302, 312
Ama87I CYCGRG 2 cut(s) 199, 566
Aor13HI TCCGGA 1 cut(s) 409
AoxI GGCC 5 cut(s) 142, 245, 288, 881, 958
ApeKI GCWGC 1 cut(s) 827
ApoI RAATTY 2 cut(s) 606, 1088
AspA2I CCTAGG 1 cut(s) 1147
AspLEI GCGC 2 cut(s) 78, 175
AspS9I GGNCC 4 cut(s) 186, 245, 881, 1151
AsuC2I CCSGG 3 cut(s) 184, 265, 1177
AsuHPI GGTGA 4 cut(s) 875, 923, 1241, 1262
AvaI CYCGRG 2 cut(s) 199, 566
AvaII GGWCC 2 cut(s) 186, 1151
AvrII CCTAGG 1 cut(s) 1147
BaeI ACNNNNGTAYC 2 cut(s) 773, 806
BamHI GGATCC 2 cut(s) 84, 304
BanII GRGCYC 3 cut(s) 276, 543, 1022
BarI GAAGNNNNNNTAC 2 cut(s) 919, 951
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 1 cut(s) 839
BccI CCATC 2 cut(s) 671, 1162
BceAI ACGGC 2 cut(s) 34, 232
BciT130I CCWGG 2 cut(s) 163, 292
BcnI CCSGG 3 cut(s) 184, 265, 1177
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 3 cut(s) 426, 717, 1148
BfuAI ACCTGC 1 cut(s) 1187
BisI GCNGC 3 cut(s) 47, 145, 828
BlnI CCTAGG 1 cut(s) 1147
BlsI GCNGC 3 cut(s) 48, 146, 829
Bme1390I CCNGG 5 cut(s) 163, 184, 265, 292, 1177
Bme18I GGWCC 2 cut(s) 186, 1151
BmeT110I CYCGRG 2 cut(s) 199, 566
BmgT120I GGNCC 4 cut(s) 186, 245, 881, 1151
BmiI GGNNCC 5 cut(s) 45, 86, 187, 273, 306
BmrFI CCNGG 5 cut(s) 163, 184, 265, 292, 1177
BmrI ACTGGG 1 cut(s) 303
BmsI GCATC 4 cut(s) 98, 634, 1133, 1183
BmuI ACTGGG 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 1008
BpuEI CTTGAG 3 cut(s) 269, 298, 527
BpuMI CCSGG 3 cut(s) 184, 265, 1177
BsaBI GATNNNNATC 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 730
BsaJI CCNNGG 5 cut(s) 50, 153, 859, 1147, 1206
BsaWI WCCGGW 1 cut(s) 409
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 4 cut(s) 309, 426, 783, 981
Bse8I GATNNNNATC 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 409
BseBI CCWGG 2 cut(s) 163, 292
BseDI CCNNGG 5 cut(s) 50, 153, 859, 1147, 1206
BseGI GGATG 3 cut(s) 406, 985, 1216
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMII CTCAG 2 cut(s) 95, 799
BseNI ACTGG 4 cut(s) 309, 426, 783, 981
BseX3I CGGCCG 1 cut(s) 142
BseXI GCAGC 1 cut(s) 839
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 5 cut(s) 144, 247, 290, 883, 960
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 543
BsiHKCI CYCGRG 2 cut(s) 199, 566
BsiSI CCGG 5 cut(s) 183, 264, 410, 957, 1176
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 4 cut(s) 18, 68, 244, 1197
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 4 cut(s) 18, 68, 244, 1197
BsmI GAATGC 2 cut(s) 646, 920
BsnI GGCC 5 cut(s) 144, 247, 290, 883, 960
BsoBI CYCGRG 2 cut(s) 199, 566
Bsp1286I GDGCHC 3 cut(s) 276, 543, 1022
Bsp13I TCCGGA 1 cut(s) 409
Bsp1407I TGTACA 1 cut(s) 1317
Bsp143I GATC 5 cut(s) 84, 225, 294, 304, 349
BspACI CCGC 6 cut(s) 47, 82, 91, 145, 257, 1293
BspANI GGCC 5 cut(s) 144, 247, 290, 883, 960
BspCNI CTCAG 2 cut(s) 96, 798
BspEI TCCGGA 1 cut(s) 409
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 2 cut(s) 235, 628
BspLI GGNNCC 5 cut(s) 45, 86, 187, 273, 306
BspMI ACCTGC 1 cut(s) 1187
BspPI GGATC 6 cut(s) 79, 92, 233, 299, 302, 312
BsrFI RCCGGY 1 cut(s) 956
BsrGI TGTACA 1 cut(s) 1317
BsrI ACTGG 4 cut(s) 309, 426, 783, 981
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 5 cut(s) 50, 153, 859, 1147, 1206
BssMI GATC 5 cut(s) 84, 225, 294, 304, 349
BssNI GRCGYC 1 cut(s) 730
BssT1I CCWWGG 2 cut(s) 153, 1147
Bst2UI CCWGG 2 cut(s) 163, 292
Bst4CI ACNGT 4 cut(s) 210, 560, 766, 860
Bst6I CTCTTC 1 cut(s) 1239
BstACI GRCGYC 1 cut(s) 730
BstAUI TGTACA 1 cut(s) 1317
BstC8I GCNNGC 3 cut(s) 80, 958, 1269
BstDEI CTNAG 3 cut(s) 104, 686, 785
BstDSI CCRYGG 2 cut(s) 50, 859
BstF5I GGATG 3 cut(s) 406, 985, 1216
BstFNI CGCG 1 cut(s) 173
BstHHI GCGC 2 cut(s) 78, 175
BstKTI GATC 5 cut(s) 87, 228, 297, 307, 352
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 5 cut(s) 84, 225, 294, 304, 349
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 3 cut(s) 43, 172, 827
BstNI CCWGG 2 cut(s) 163, 292
BstSCI CCNGG 5 cut(s) 161, 182, 263, 290, 1175
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 1 cut(s) 839
BstX2I RGATCY 3 cut(s) 84, 294, 304
BstXI CCANNNNNNTGG 1 cut(s) 976
BstYI RGATCY 3 cut(s) 84, 294, 304
BstZI CGGCCG 1 cut(s) 142
BsuRI GGCC 5 cut(s) 144, 247, 290, 883, 960
BtgI CCRYGG 2 cut(s) 50, 859
BtsCI GGATG 3 cut(s) 406, 985, 1216
BtsIMutI CAGTG 4 cut(s) 316, 535, 565, 974
BveI ACCTGC 1 cut(s) 1187
Cac8I GCNNGC 3 cut(s) 80, 958, 1269
CciI TCATGA 2 cut(s) 235, 628
CfoI GCGC 2 cut(s) 78, 175
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 4 cut(s) 186, 245, 881, 1151
CseI GACGC 1 cut(s) 605
Csp6I GTAC 3 cut(s) 211, 782, 1318
CviAII CATG 6 cut(s) 14, 64, 236, 629, 709, 896
CviQI GTAC 3 cut(s) 211, 782, 1318
DdeI CTNAG 3 cut(s) 104, 686, 785
DpnI GATC 5 cut(s) 86, 227, 296, 306, 351
DpnII GATC 5 cut(s) 84, 225, 294, 304, 349
DraIII CACNNNGTG 1 cut(s) 518
DrdI GACNNNNNNGTC 1 cut(s) 947
DseDI GACNNNNNNGTC 1 cut(s) 947
EaeI YGGCCR 1 cut(s) 142
EagI CGGCCG 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 1239
EarI CTCTTC 1 cut(s) 1239
Ecl136II GAGCTC 1 cut(s) 541
EclXI CGGCCG 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 153, 1147
Eco147I AGGCCT 1 cut(s) 290
Eco24I GRGCYC 3 cut(s) 276, 543, 1022
Eco47I GGWCC 2 cut(s) 186, 1151
Eco52I CGGCCG 1 cut(s) 142
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 1125
Eco88I CYCGRG 2 cut(s) 199, 566
EcoICRI GAGCTC 1 cut(s) 541
EcoRI GAATTC 1 cut(s) 606
EcoRII CCWGG 2 cut(s) 161, 290
EcoT14I CCWWGG 2 cut(s) 153, 1147
EcoT22I ATGCAT 2 cut(s) 901, 920
EcoT38I GRGCYC 3 cut(s) 276, 543, 1022
ErhI CCWWGG 2 cut(s) 153, 1147
FaeI CATG 6 cut(s) 17, 67, 239, 632, 712, 899
FaqI GGGAC 4 cut(s) 18, 68, 244, 1197
FatI CATG 6 cut(s) 13, 63, 235, 628, 708, 895
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 3 cut(s) 47, 145, 828
FokI GGATG 3 cut(s) 393, 992, 1223
FriOI GRGCYC 3 cut(s) 276, 543, 1022
Fsp4HI GCNGC 3 cut(s) 47, 145, 828
FspBI CTAG 3 cut(s) 426, 717, 1148
GlaI GCGC 2 cut(s) 77, 174
GluI GCNGC 3 cut(s) 47, 145, 828
GsuI CTGGAG 1 cut(s) 1008
HaeIII GGCC 5 cut(s) 144, 247, 290, 883, 960
HapII CCGG 5 cut(s) 183, 264, 410, 957, 1176
HgaI GACGC 1 cut(s) 605
HhaI GCGC 2 cut(s) 78, 175
Hin1I GRCGYC 1 cut(s) 730
Hin1II CATG 6 cut(s) 17, 67, 239, 632, 712, 899
Hin6I GCGC 2 cut(s) 76, 173
HinP1I GCGC 2 cut(s) 76, 173
HincII GTYRAC 2 cut(s) 334, 1099
HindII GTYRAC 2 cut(s) 334, 1099
HinfI GANTC 8 cut(s) 197, 232, 239, 260, 280, 659, 796, 1051
HpaII CCGG 5 cut(s) 183, 264, 410, 957, 1176
HphI GGTGA 4 cut(s) 875, 923, 1241, 1262
Hpy166II GTNNAC 4 cut(s) 334, 1099, 1170, 1333
Hpy188I TCNGA 4 cut(s) 190, 271, 940, 1207
Hpy188III TCNNGA 9 cut(s) 118, 223, 229, 236, 410, 566, 629, 717, 1025
Hpy8I GTNNAC 4 cut(s) 334, 1099, 1170, 1333
HpyAV CCTTC 3 cut(s) 379, 1149, 1174
HpyCH4III ACNGT 4 cut(s) 210, 560, 766, 860
HpyCH4IV ACGT 2 cut(s) 730, 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 172, 827
HpyF3I CTNAG 3 cut(s) 104, 686, 785
HpySE526I ACGT 2 cut(s) 730, 813
Hsp92I GRCGYC 1 cut(s) 730
Hsp92II CATG 6 cut(s) 17, 67, 239, 632, 712, 899
HspAI GCGC 2 cut(s) 76, 173
Kpn2I TCCGGA 1 cut(s) 409
KroI GCCGGC 1 cut(s) 956
KroNI GCCGGC 1 cut(s) 958
Kzo9I GATC 5 cut(s) 84, 225, 294, 304, 349
LmnI GCTCC 6 cut(s) 17, 34, 43, 116, 271, 538
Lsp1109I GCAGC 1 cut(s) 839
LweI GCATC 4 cut(s) 98, 634, 1133, 1183
MaeI CTAG 3 cut(s) 426, 717, 1148
MaeII ACGT 2 cut(s) 730, 813
MaeIII GTNAC 3 cut(s) 441, 681, 1106
MalI GATC 5 cut(s) 86, 227, 296, 306, 351
MboI GATC 5 cut(s) 84, 225, 294, 304, 349
MboII GAAGA 7 cut(s) 79, 82, 344, 658, 1097, 1256, 1291
MfeI CAATTG 2 cut(s) 699, 1313
MflI RGATCY 3 cut(s) 84, 294, 304
MhlI GDGCHC 3 cut(s) 276, 543, 1022
MlyI GAGTC 5 cut(s) 191, 226, 289, 668, 805
MmeI TCCRAC 3 cut(s) 213, 249, 771
MnlI CCTC 8 cut(s) 502, 903, 1059, 1125, 1201, 1219, 1240, 1246
Mph1103I ATGCAT 2 cut(s) 901, 920
MroI TCCGGA 1 cut(s) 409
MroNI GCCGGC 1 cut(s) 956
MseI TTAA 5 cut(s) 366, 555, 735, 768, 840
MslI CAYNNNNRTG 2 cut(s) 450, 1191
MspI CCGG 5 cut(s) 183, 264, 410, 957, 1176
MspR9I CCNGG 5 cut(s) 163, 184, 265, 292, 1177
MunI CAATTG 2 cut(s) 699, 1313
Mva1269I GAATGC 2 cut(s) 646, 920
MvaI CCWGG 2 cut(s) 163, 292
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 43, 172, 827
NaeI GCCGGC 1 cut(s) 958
NciI CCSGG 3 cut(s) 184, 265, 1177
NdeII GATC 5 cut(s) 84, 225, 294, 304, 349
NgoMIV GCCGGC 1 cut(s) 956
NlaIII CATG 6 cut(s) 17, 67, 239, 632, 712, 899
NlaIV GGNNCC 5 cut(s) 45, 86, 187, 273, 306
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 441
NsiI ATGCAT 2 cut(s) 901, 920
OliI CACNNNNGTG 1 cut(s) 450
PagI TCATGA 2 cut(s) 235, 628
PceI AGGCCT 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PctI GAATGC 2 cut(s) 646, 920
PdiI GCCGGC 1 cut(s) 958
PfeI GAWTC 3 cut(s) 239, 260, 1051
Pfl23II CGTACG 1 cut(s) 210
PkrI GCNGC 3 cut(s) 48, 146, 829
PleI GAGTC 5 cut(s) 191, 226, 288, 667, 804
PpsI GAGTC 5 cut(s) 191, 226, 288, 667, 804
Psp124BI GAGCTC 1 cut(s) 543
Psp6I CCWGG 2 cut(s) 161, 290
PspGI CCWGG 2 cut(s) 161, 290
PspLI CGTACG 1 cut(s) 210
PspN4I GGNNCC 5 cut(s) 45, 86, 187, 273, 306
PspPI GGNCC 4 cut(s) 186, 245, 881, 1151
PsrI GAACNNNNNNTAC 4 cut(s) 203, 235, 765, 797
PsuI RGATCY 3 cut(s) 84, 294, 304
RsaI GTAC 3 cut(s) 212, 783, 1319
RsaNI GTAC 3 cut(s) 211, 782, 1318
RseI CAYNNNNRTG 2 cut(s) 450, 1191
SacI GAGCTC 1 cut(s) 543
SalI GTCGAC 1 cut(s) 1097
SaqAI TTAA 5 cut(s) 366, 555, 735, 768, 840
SatI GCNGC 3 cut(s) 47, 145, 828
Sau3AI GATC 5 cut(s) 84, 225, 294, 304, 349
Sau96I GGNCC 4 cut(s) 186, 245, 881, 1151
SchI GAGTC 5 cut(s) 191, 226, 289, 668, 805
ScrFI CCNGG 5 cut(s) 163, 184, 265, 292, 1177
SduI GDGCHC 3 cut(s) 276, 543, 1022
SfaNI GCATC 4 cut(s) 98, 634, 1133, 1183
SinI GGWCC 2 cut(s) 186, 1151
SmiMI CAYNNNNRTG 2 cut(s) 450, 1191
SmlI CTYRAG 3 cut(s) 277, 284, 542
SmoI CTYRAG 3 cut(s) 277, 284, 542
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 6 cut(s) 47, 82, 91, 145, 257, 1293
SspMI CTAG 3 cut(s) 426, 717, 1148
SstI GAGCTC 1 cut(s) 543
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 5 cut(s) 161, 182, 263, 290, 1175
StyI CCWWGG 2 cut(s) 153, 1147
TaaI ACNGT 4 cut(s) 210, 560, 766, 860
TaiI ACGT 2 cut(s) 733, 816
TaqI TCGA 5 cut(s) 195, 395, 507, 673, 1098
TatI WGTACW 1 cut(s) 1317
TauI GCSGC 2 cut(s) 49, 147
TfiI GAWTC 3 cut(s) 239, 260, 1051
Tru1I TTAA 5 cut(s) 366, 555, 735, 768, 840
Tru9I TTAA 5 cut(s) 366, 555, 735, 768, 840
TscAI CASTG 4 cut(s) 316, 535, 565, 981
TseFI GTSAC 1 cut(s) 441
TseI GCWGC 1 cut(s) 827
Tsp45I GTSAC 1 cut(s) 441
TspDTI ATGAA 3 cut(s) 80, 252, 903
TspGWI ACGGA 4 cut(s) 103, 345, 515, 941
TspRI CASTG 4 cut(s) 316, 535, 565, 981
VpaK11BI GGWCC 2 cut(s) 186, 1151
XapI RAATTY 2 cut(s) 606, 1088
XbaI TCTAGA 1 cut(s) 716
XmaJI CCTAGG 1 cut(s) 1147
XmiI GTMKAC 1 cut(s) 1098
XspI CTAG 3 cut(s) 426, 717, 1148
ZraI GACGTC 1 cut(s) 731
Zsp2I ATGCAT 2 cut(s) 901, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.