Rh6CG479600

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001016
Physical Location & Seq
Forward (+)
64697487 .. 64699086
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG479600.1

Sequence Viewer

Length: 1182 bp
ATGGAGATGGAGAGGCAGGGGAGTGTTCAGAAGCTGAAATGCACTGTCCAGAACTACGAATGGGGCAAGAAAGGCCTAGATTCTGAGGTAGCAAGGCTTTGTGCCTCCAACTCTGGTAGTAAAATTGACCCACAAAAGCCTTACGCCGAGTTTTGGATGGGAACCCATCAATCTGGACCTTCCTTTTTGATTGATCCGAATAACACTACGTCGTCGTTGTCTCTCAAAGACTGGATCTCTGACAAACCAGATGAGATTCTTGGTCACAGGGTCGTCCAAAAATGGGGCTCTGACCTACCTTTCTTGTTTAAGGTACCCGGAGATGCGCTTGCTACAACACATTTTCAAGCTCTTTGTGGTTTCATTGCTTTCCAGGAGCTTAAGGATGTGCTCAACAACGTACCCGAGATTGTGCAAGTGGTTGGAAGTGAAGTTGCAAACCAAGTGCTTCAGACCGATGACGAAGATGGGGTGGGGAGCGGAGAAAAAGTCAAGTCTGTTCTGCGTTCAATTTTCAGCCAGTTAATGTCAGCTGACAAACAGATGATAACTGCAGCAACATCTAAACTGAAAAATCGTCTACAAACAGAAAGCCAGGTGAGGCAGTTGACATACAAGGAGCAGCTGGTGTTGCAATTGGAAGAGCAATATTCAGAAGAGGACGTGGGTGTGATATCTGCTTTCTTTCTCAACCATGTGAAGCGTAATCCTGGAGAAGCATTGTGCCTCGGAGCAAATGAACCTCATGCCTATGTATCCGGGGACTGCATTGAGTGCATGGCGACCTCGGACAACGTAGTCAGGGCTGGCCTTACTCCTAAGCATCGAGATGCTAAGACCCTTTGTTCTATGCTCACATATAAGCAGGGTTTCCCTGAAATCATGCGAGGAGTTGCCATGAATCCTTATGTGACAAGGTACATCCCGCCTTTCGATGAATTTGAGGTGGATCGGTGTCATCTTCCGGGTGGGGAATCGACGGAGTTTCCTGCAGTAGCTGGTCCTTCAATTTTTGTGGTCACTCTTGGAAAGGGAACAATATGCACTTGTAATGGAGATAATGAAATTATCCAAGGAGATGTTCTGTTTGCATGTGCAGATACTCGGATTAGCGTAACGAGTGCATCTCAGTTGCATGTATATAGAGCCGGAGTGAACAGCATGGTCTTTGGGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

393

Amino Acids

43.15

Weight (kDa)

5.22

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 9 - 105 9.1e-21 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 149 - 230 1.1e-10 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 797
Acc65I GGTACC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 313
AccBSI CCGCTC 1 cut(s) 480
AccI GTMKAC 1 cut(s) 580
AciI CCGC 2 cut(s) 480, 926
AclWI GGATC 3 cut(s) 188, 242, 957
AcsI RAATTY 1 cut(s) 938
AcuI CTGAAG 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 1175
AfaI GTAC 3 cut(s) 315, 402, 920
AfiI CCNNNNNNNGG 2 cut(s) 153, 283
AflII CTTAAG 1 cut(s) 380
AgsI TTSAA 3 cut(s) 347, 510, 1008
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 3 cut(s) 372, 594, 709
AjuI GAANNNNNNNTTGG 2 cut(s) 1065, 1097
AluBI AGCT 6 cut(s) 34, 350, 379, 533, 625, 998
AluI AGCT 6 cut(s) 34, 350, 379, 533, 625, 998
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 3 cut(s) 188, 242, 957
AlwNI CAGNNNCTG 2 cut(s) 34, 998
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 2 cut(s) 73, 808
ApeKI GCWGC 2 cut(s) 554, 622
ApoI RAATTY 1 cut(s) 938
Asp718I GGTACC 1 cut(s) 313
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 2 cut(s) 176, 1001
AsuC2I CCSGG 3 cut(s) 318, 760, 966
AsuHPI GGTGA 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 2 cut(s) 176, 1001
BanI GGYRCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 566, 634
BccI CCATC 3 cut(s) 151, 174, 461
BciT130I CCWGG 3 cut(s) 374, 596, 711
BciVI GTATCC 1 cut(s) 766
BcnI CCSGG 3 cut(s) 318, 760, 966
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 77
BfmI CTRYAG 2 cut(s) 552, 990
BfrI CTTAAG 1 cut(s) 380
BfuI GTATCC 1 cut(s) 766
BisI GCNGC 2 cut(s) 555, 623
BlsI GCNGC 2 cut(s) 556, 624
Bme1390I CCNGG 6 cut(s) 318, 374, 596, 711, 760, 966
Bme18I GGWCC 2 cut(s) 176, 1001
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 2 cut(s) 176, 1001
BmiI GGNNCC 2 cut(s) 163, 315
BmrFI CCNGG 6 cut(s) 318, 374, 596, 711, 760, 966
BmsI GCATC 4 cut(s) 313, 820, 832, 1133
BplI GAGNNNNNCTC 2 cut(s) 1111, 1143
BpmI CTGGAG 1 cut(s) 732
Bpu10I CCTNAGC 1 cut(s) 819
BpuMI CCSGG 3 cut(s) 318, 760, 966
BsaHI GRCGYC 1 cut(s) 1175
BsaJI CCNNGG 4 cut(s) 727, 759, 786, 1072
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 283
Bse1I ACTGG 2 cut(s) 236, 520
Bse3DI GCAATG 1 cut(s) 363
BseBI CCWGG 3 cut(s) 374, 596, 711
BseDI CCNNGG 4 cut(s) 727, 759, 786, 1072
BseGI GGATG 3 cut(s) 162, 391, 921
BseLI CCNNNNNNNGG 2 cut(s) 153, 283
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 2 cut(s) 75, 1142
BseNI ACTGG 2 cut(s) 236, 520
BseRI GAGGAG 1 cut(s) 903
BseXI GCAGC 2 cut(s) 566, 634
BsgI GTGCAG 1 cut(s) 1116
BshFI GGCC 2 cut(s) 75, 810
BshNI GGYRCC 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 4 cut(s) 318, 759, 965, 1149
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 2 cut(s) 153, 283
BsmAI GTCTC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 776
BsnI GGCC 2 cut(s) 75, 810
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 290, 393
Bsp143I GATC 3 cut(s) 193, 234, 949
BspACI CCGC 2 cut(s) 480, 926
BspANI GGCC 2 cut(s) 75, 810
BspCNI CTCAG 2 cut(s) 76, 1141
BspLI GGNNCC 2 cut(s) 163, 315
BspMAI CTGCAG 2 cut(s) 556, 994
BspPI GGATC 3 cut(s) 188, 242, 957
BspQI GCTCTTC 1 cut(s) 636
BspT107I GGYRCC 1 cut(s) 313
BspTI CTTAAG 1 cut(s) 380
BsrBI CCGCTC 1 cut(s) 480
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 2 cut(s) 236, 520
BssECI CCNNGG 4 cut(s) 727, 759, 786, 1072
BssMI GATC 3 cut(s) 193, 234, 949
BssNI GRCGYC 1 cut(s) 1175
BssT1I CCWWGG 1 cut(s) 1072
Bst2UI CCWGG 3 cut(s) 374, 596, 711
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 2 cut(s) 636, 651
BstACI GRCGYC 1 cut(s) 1175
BstAFI CTTAAG 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 774
BstC8I GCNNGC 2 cut(s) 330, 808
BstDEI CTNAG 4 cut(s) 84, 819, 834, 1128
BstF5I GGATG 3 cut(s) 162, 391, 921
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 196, 237, 952
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 3 cut(s) 193, 234, 949
BstMWI GCNNNNNNNGC 3 cut(s) 72, 631, 774
BstNI CCWGG 3 cut(s) 374, 596, 711
BstNSI RCATGY 2 cut(s) 1095, 1139
BstSCI CCNGG 6 cut(s) 316, 372, 594, 709, 758, 964
BstSFI CTRYAG 2 cut(s) 552, 990
BstV1I GCAGC 2 cut(s) 566, 634
BstX2I RGATCY 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 1 cut(s) 234
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 2 cut(s) 75, 810
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 3 cut(s) 162, 391, 921
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 2 cut(s) 330, 808
CaiI CAGNNNCTG 2 cut(s) 34, 998
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 2 cut(s) 176, 1001
CseI GACGC 1 cut(s) 1164
Csp6I GTAC 3 cut(s) 314, 401, 919
CspCI CAANNNNNGTGG 2 cut(s) 996, 1031
CviAII CATG 8 cut(s) 695, 746, 778, 883, 898, 1092, 1136, 1162
CviQI GTAC 3 cut(s) 314, 401, 919
DdeI CTNAG 4 cut(s) 84, 819, 834, 1128
DpnI GATC 3 cut(s) 195, 236, 951
DpnII GATC 3 cut(s) 193, 234, 949
DrdI GACNNNNNNGTC 1 cut(s) 797
DseDI GACNNNNNNGTC 1 cut(s) 797
Eam1104I CTCTTC 2 cut(s) 636, 651
EarI CTCTTC 2 cut(s) 636, 651
Eco130I CCWWGG 1 cut(s) 1072
Eco147I AGGCCT 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 290
Eco32I GATATC 1 cut(s) 675
Eco47I GGWCC 2 cut(s) 176, 1001
Eco57I CTGAAG 1 cut(s) 434
Eco88I CYCGRG 1 cut(s) 404
EcoRII CCWGG 3 cut(s) 372, 594, 709
EcoRV GATATC 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 1072
EcoT38I GRGCYC 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 1072
FaeI CATG 8 cut(s) 698, 749, 781, 886, 901, 1095, 1139, 1165
FaqI GGGAC 1 cut(s) 776
FatI CATG 8 cut(s) 694, 745, 777, 882, 897, 1091, 1135, 1161
FauI CCCGC 1 cut(s) 933
FblI GTMKAC 1 cut(s) 580
Fnu4HI GCNGC 2 cut(s) 555, 623
FokI GGATG 3 cut(s) 169, 398, 908
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 2 cut(s) 555, 623
FspBI CTAG 1 cut(s) 77
GlaI GCGC 1 cut(s) 327
GluI GCNGC 2 cut(s) 555, 623
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 2 cut(s) 75, 810
HapII CCGG 4 cut(s) 318, 759, 965, 1149
HgaI GACGC 1 cut(s) 1164
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 1175
Hin1II CATG 8 cut(s) 698, 749, 781, 886, 901, 1095, 1139, 1165
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 4 cut(s) 80, 256, 901, 974
HpaII CCGG 4 cut(s) 318, 759, 965, 1149
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 3 cut(s) 581, 609, 1156
Hpy188III TCNNGA 4 cut(s) 49, 174, 827, 1179
Hpy8I GTNNAC 3 cut(s) 581, 609, 1156
Hpy99I CGWCG 3 cut(s) 214, 217, 982
HpyAV CCTTC 2 cut(s) 189, 1014
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 4 cut(s) 209, 399, 663, 795
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 631, 774
HpyF3I CTNAG 4 cut(s) 84, 819, 834, 1128
HpySE526I ACGT 4 cut(s) 209, 399, 663, 795
Hsp92I GRCGYC 1 cut(s) 1175
Hsp92II CATG 8 cut(s) 698, 749, 781, 886, 901, 1095, 1139, 1165
HspAI GCGC 1 cut(s) 326
KpnI GGTACC 1 cut(s) 317
Kzo9I GATC 3 cut(s) 193, 234, 949
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 4 cut(s) 376, 477, 619, 731
Lsp1109I GCAGC 2 cut(s) 566, 634
LweI GCATC 4 cut(s) 313, 820, 832, 1133
MaeI CTAG 1 cut(s) 77
MaeII ACGT 4 cut(s) 209, 399, 663, 795
MaeIII GTNAC 4 cut(s) 263, 910, 1018, 1114
MalI GATC 3 cut(s) 195, 236, 951
MbiI CCGCTC 1 cut(s) 480
MboI GATC 3 cut(s) 193, 234, 949
MboII GAAGA 4 cut(s) 476, 653, 668, 953
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 2 cut(s) 290, 393
MluCI AATT 6 cut(s) 123, 510, 635, 938, 1008, 1065
MmeI TCCRAC 2 cut(s) 132, 403
MseI TTAA 3 cut(s) 309, 381, 524
MslI CAYNNNNRTG 2 cut(s) 750, 828
MspA1I CMGCKG 2 cut(s) 533, 625
MspCI CTTAAG 1 cut(s) 380
MspI CCGG 4 cut(s) 318, 759, 965, 1149
MspR9I CCNGG 6 cut(s) 318, 374, 596, 711, 760, 966
MunI CAATTG 1 cut(s) 635
MvaI CCWGG 3 cut(s) 374, 596, 711
MwoI GCNNNNNNNGC 3 cut(s) 72, 631, 774
NciI CCSGG 3 cut(s) 318, 760, 966
NdeII GATC 3 cut(s) 193, 234, 949
NlaIII CATG 8 cut(s) 698, 749, 781, 886, 901, 1095, 1139, 1165
NlaIV GGNNCC 2 cut(s) 163, 315
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 3 cut(s) 263, 910, 1018
NspI RCATGY 2 cut(s) 1095, 1139
PceI AGGCCT 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 636
PfeI GAWTC 4 cut(s) 80, 256, 901, 974
PfoI TCCNGGA 2 cut(s) 372, 709
PkrI GCNGC 2 cut(s) 556, 624
Psp6I CCWGG 3 cut(s) 372, 594, 709
PspGI CCWGG 3 cut(s) 372, 594, 709
PspN4I GGNNCC 2 cut(s) 163, 315
PspPI GGNCC 2 cut(s) 176, 1001
PstI CTGCAG 2 cut(s) 556, 994
PstNI CAGNNNCTG 2 cut(s) 34, 998
PsuI RGATCY 1 cut(s) 234
PvuII CAGCTG 2 cut(s) 533, 625
RsaI GTAC 3 cut(s) 315, 402, 920
RsaNI GTAC 3 cut(s) 314, 401, 919
RseI CAYNNNNRTG 2 cut(s) 750, 828
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 3 cut(s) 309, 381, 524
SatI GCNGC 2 cut(s) 555, 623
Sau3AI GATC 3 cut(s) 193, 234, 949
Sau96I GGNCC 2 cut(s) 176, 1001
ScrFI CCNGG 6 cut(s) 318, 374, 596, 711, 760, 966
SduI GDGCHC 2 cut(s) 290, 393
SfaNI GCATC 4 cut(s) 313, 820, 832, 1133
SfcI CTRYAG 2 cut(s) 552, 990
SinI GGWCC 2 cut(s) 176, 1001
SmiMI CAYNNNNRTG 2 cut(s) 750, 828
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 6 cut(s) 123, 510, 635, 938, 1008, 1065
SseBI AGGCCT 1 cut(s) 75
SsiI CCGC 2 cut(s) 480, 926
SspI AATATT 1 cut(s) 650
SspMI CTAG 1 cut(s) 77
StuI AGGCCT 1 cut(s) 75
StyD4I CCNGG 6 cut(s) 316, 372, 594, 709, 758, 964
StyI CCWWGG 1 cut(s) 1072
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 4 cut(s) 212, 402, 666, 798
TaqI TCGA 3 cut(s) 826, 933, 977
TaqII GACCGA 1 cut(s) 470
TasI AATT 6 cut(s) 123, 510, 635, 938, 1008, 1065
TfiI GAWTC 4 cut(s) 80, 256, 901, 974
Tru1I TTAA 3 cut(s) 309, 381, 524
Tru9I TTAA 3 cut(s) 309, 381, 524
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 3 cut(s) 263, 910, 1018
TseI GCWGC 2 cut(s) 554, 622
Tsp45I GTSAC 3 cut(s) 263, 910, 1018
TspDTI ATGAA 5 cut(s) 352, 753, 914, 951, 1077
TspGWI ACGGA 1 cut(s) 995
TspRI CASTG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 380
VpaK11BI GGWCC 2 cut(s) 176, 1001
XapI RAATTY 1 cut(s) 938
XceI RCATGY 2 cut(s) 1095, 1139
XmiI GTMKAC 1 cut(s) 580
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.