RLG00000003239

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001016
Physical Location & Seq
Forward (+)
45947744 .. 45950146
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003239

Sequence Viewer

Length: 1305 bp
ATGGAGAAGTCATTGATTCACAAATCAATGAAGCCGAGGCAGAGGAGTGTTCAGAGGCTGAGCTGCTCTGTTCAGAACTATGATTGGGGGAAGAAAGGCCCGGACTCTGAGGTGGCCAGGCTCTATGCCTCCAATTCTGGCTCTGAAATTGACCCGGAAAAGCCTTATGCCGAGTTTTGGATGGGAACCCATGATTCTGGACCCTCATTCTTGACTCATCAGGATCTCAATGGCAAGTCTATGTCCCTCAAAGATTGGGTTTCAGCCAACTCAAATGAGCTGCTTGGTCATAAAGTCGTCCAAAAGTGGGGCTCTGACCTCCCTTTCTTGTTTAAGGTGCTTTCTGTGAGAAAGGCATTGTCGATACAGGCACATCCGGATAAGGACTTGGCCAAGGTTTTGCACAAGTTTATGCCAAGTGCTTACAAGGACGATAATCACAAGCCTGAGATGGCACTTGCTATAACACATTTTCAGGCTCTTTGTGGCTTTATCACTCTTGAGGAGCTTAAGGGTGTGCTTGATAATGTCCCTGAGATTGTACAAGTGGTTGGTAGTGAAGTAGCAAACCAAGTGTTTGACACCACAAATGCAGATGGGGAAGATAAAGTCAAGTCTGTTCTGCGTTTGATTTTTACCCAACTCATGTCAGCAACCAAAGAGATTGTAACTACAGCAACAACTAAACTGAAAAATCGTTTACACATGGAAAATCAGGTGAGGCAGTTGACAGAAAAAGAGCAACTGGTTCTGGAATTGGAAAGGCAATATCCAGATGATGTGGGTGTCATGTCTGCTTTCTTTCTCAACTATGTCAAGCTTAATCCAGGAGAAGCACTATACCTCGGAGCAAATGAACCCCATGCCTATATGTTCGGTGACTGCATCGAGTGCATGGCAACTTCTGACAATGTGGTCAGGGCCGGCCTCACTCCCAAGCATCGGGATGTGAAAACCCTTTGTTCTATGCTCACATACAAGCAGGGCTGCCCTGAAATCCTGAAAGGAGTTGCCTTAAATTCTTATGTGACAAGGTACCTCCCACCTTTTGACGAATTTGAGGTTGATCGATGCCATCTTCCCCAGGGAGAATCTGTGGAATTTCCTGCAGTCCAAGGTCCTTCCATTTTCGTCGTCACTTTTGGGGAGGGAATCATATATACAAGCAATTTTACAGGAGATATAAATATCACACAAGGAGAGGTTCTTTTCGTGCCTGCAGATACTGAGATTAGCATAACAAGTGCATCTGAGTTGCACATATATAGAGCCGGAGTGAACAGCATGTTCTTTCAAGTCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

435

Amino Acids

48.54

Weight (kDa)

5.67

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 17 - 164 1.5e-50 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 184 - 269 2.1e-13 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 914
Acc65I GGTACC 1 cut(s) 1035
AccB1I GGYRCC 1 cut(s) 1035
AccIII TCCGGA 1 cut(s) 376
AclWI GGATC 1 cut(s) 231
AcoI YGGCCR 2 cut(s) 114, 390
AcsI RAATTY 3 cut(s) 1018, 1055, 1100
AfaI GTAC 2 cut(s) 543, 1037
AfiI CCNNNNNNNGG 3 cut(s) 177, 307, 942
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 1 cut(s) 1295
AjnI CCWGG 3 cut(s) 116, 826, 1083
AluBI AGCT 4 cut(s) 63, 280, 508, 820
AluI AGCT 4 cut(s) 63, 280, 508, 820
AlwI GGATC 1 cut(s) 231
AlwNI CAGNNNCTG 2 cut(s) 58, 1226
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 5 cut(s) 97, 114, 390, 921, 925
ApeKI GCWGC 3 cut(s) 63, 280, 987
ApoI RAATTY 3 cut(s) 1018, 1055, 1100
Asp718I GGTACC 1 cut(s) 1035
AspS9I GGNCC 4 cut(s) 98, 200, 921, 1118
AsuC2I CCSGG 2 cut(s) 101, 155
AsuHPI GGTGA 2 cut(s) 730, 890
AvaII GGWCC 2 cut(s) 200, 1118
BalI TGGCCA 2 cut(s) 116, 392
BanI GGYRCC 1 cut(s) 1035
BanII GRGCYC 1 cut(s) 314
BbvI GCAGC 3 cut(s) 50, 267, 974
BccI CCATC 4 cut(s) 175, 445, 590, 1083
BciT130I CCWGG 3 cut(s) 118, 828, 1085
BcnI CCSGG 2 cut(s) 101, 155
BfmI CTRYAG 3 cut(s) 672, 1107, 1218
BfrI CTTAAG 1 cut(s) 509
BisI GCNGC 3 cut(s) 64, 281, 988
BlpI GCTNAGC 1 cut(s) 59
BlsI GCNGC 3 cut(s) 65, 282, 989
Bme1390I CCNGG 5 cut(s) 101, 118, 155, 828, 1085
Bme18I GGWCC 2 cut(s) 200, 1118
BmgT120I GGNCC 4 cut(s) 98, 200, 921, 1118
BmiI GGNNCC 3 cut(s) 187, 202, 1037
BmrFI CCNGG 5 cut(s) 101, 118, 155, 828, 1085
BmsI GCATC 4 cut(s) 894, 949, 1061, 1256
Bpu1102I GCTNAGC 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 2 cut(s) 101, 155
Bsa29I ATCGAT 1 cut(s) 1069
BsaJI CCNNGG 6 cut(s) 35, 393, 844, 1083, 1084, 1114
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 1080, 1110
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 307, 942
Bse118I RCCGGY 1 cut(s) 923
Bse1I ACTGG 1 cut(s) 750
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 3 cut(s) 118, 828, 1085
BseCI ATCGAT 1 cut(s) 1069
BseDI CCNNGG 6 cut(s) 35, 393, 844, 1083, 1084, 1114
BseGI GGATG 3 cut(s) 186, 373, 952
BseLI CCNNNNNNNGG 3 cut(s) 177, 307, 942
BseMII CTCAG 6 cut(s) 50, 99, 438, 525, 1218, 1242
BseNI ACTGG 1 cut(s) 750
BseRI GAGGAG 2 cut(s) 58, 518
BseXI GCAGC 3 cut(s) 50, 267, 974
BshFI GGCC 5 cut(s) 99, 116, 392, 923, 927
BshNI GGYRCC 1 cut(s) 1035
BshVI ATCGAT 1 cut(s) 1069
BsiSI CCGG 5 cut(s) 101, 155, 377, 924, 1272
BslFI GGGAC 3 cut(s) 229, 515, 1283
BslI CCNNNNNNNGG 3 cut(s) 177, 307, 942
BsmFI GGGAC 3 cut(s) 229, 515, 1283
BsnI GGCC 5 cut(s) 99, 116, 392, 923, 927
Bsp1286I GDGCHC 1 cut(s) 314
Bsp13I TCCGGA 1 cut(s) 376
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 2 cut(s) 223, 1066
Bsp1720I GCTNAGC 1 cut(s) 59
BspANI GGCC 5 cut(s) 99, 116, 392, 923, 927
BspCNI CTCAG 6 cut(s) 51, 100, 439, 526, 1219, 1243
BspDI ATCGAT 1 cut(s) 1069
BspEI TCCGGA 1 cut(s) 376
BspLI GGNNCC 3 cut(s) 187, 202, 1037
BspMAI CTGCAG 2 cut(s) 1111, 1222
BspPI GGATC 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 1035
BspTI CTTAAG 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 923
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 1 cut(s) 750
BssAI RCCGGY 1 cut(s) 923
BssECI CCNNGG 6 cut(s) 35, 393, 844, 1083, 1084, 1114
BssMI GATC 2 cut(s) 223, 1066
BssT1I CCWWGG 2 cut(s) 393, 1114
Bst2UI CCWGG 3 cut(s) 118, 828, 1085
BstAFI CTTAAG 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 891
BstAUI TGTACA 1 cut(s) 541
BstC8I GCNNGC 2 cut(s) 925, 1218
BstDEI CTNAG 6 cut(s) 59, 108, 447, 534, 1227, 1251
BstF5I GGATG 3 cut(s) 186, 373, 952
BstKTI GATC 2 cut(s) 226, 1069
BstMBI GATC 2 cut(s) 223, 1066
BstMWI GCNNNNNNNGC 1 cut(s) 891
BstNI CCWGG 3 cut(s) 118, 828, 1085
BstNSI RCATGY 1 cut(s) 1288
BstSCI CCNGG 5 cut(s) 99, 116, 153, 826, 1083
BstSFI CTRYAG 3 cut(s) 672, 1107, 1218
BstV1I GCAGC 3 cut(s) 50, 267, 974
BstX2I RGATCY 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 1069
BsuRI GGCC 5 cut(s) 99, 116, 392, 923, 927
BsuTUI ATCGAT 1 cut(s) 1069
BtsCI GGATG 3 cut(s) 186, 373, 952
Cac8I GCNNGC 2 cut(s) 925, 1218
CaiI CAGNNNCTG 2 cut(s) 58, 1226
Cfr10I RCCGGY 1 cut(s) 923
Cfr13I GGNCC 4 cut(s) 98, 200, 921, 1118
ClaI ATCGAT 1 cut(s) 1069
Csp6I GTAC 2 cut(s) 542, 1036
CviAII CATG 7 cut(s) 191, 646, 706, 790, 863, 895, 1285
CviQI GTAC 2 cut(s) 542, 1036
DdeI CTNAG 6 cut(s) 59, 108, 447, 534, 1227, 1251
DpnI GATC 2 cut(s) 225, 1068
DpnII GATC 2 cut(s) 223, 1066
DrdI GACNNNNNNGTC 1 cut(s) 914
DseDI GACNNNNNNGTC 1 cut(s) 914
EaeI YGGCCR 2 cut(s) 114, 390
Eco130I CCWWGG 2 cut(s) 393, 1114
Eco24I GRGCYC 1 cut(s) 314
Eco47I GGWCC 2 cut(s) 200, 1118
EcoO109I RGGNCCY 1 cut(s) 1118
EcoRII CCWGG 3 cut(s) 116, 826, 1083
EcoT14I CCWWGG 2 cut(s) 393, 1114
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 2 cut(s) 393, 1114
FaeI CATG 7 cut(s) 194, 649, 709, 793, 866, 898, 1288
FaqI GGGAC 3 cut(s) 229, 515, 1283
FatI CATG 7 cut(s) 190, 645, 705, 789, 862, 894, 1284
Fnu4HI GCNGC 3 cut(s) 64, 281, 988
FokI GGATG 3 cut(s) 193, 360, 959
FriOI GRGCYC 1 cut(s) 314
FseI GGCCGGCC 1 cut(s) 927
Fsp4HI GCNGC 3 cut(s) 64, 281, 988
GluI GCNGC 3 cut(s) 64, 281, 988
HaeIII GGCC 5 cut(s) 99, 116, 392, 923, 927
HapII CCGG 5 cut(s) 101, 155, 377, 924, 1272
Hin1II CATG 7 cut(s) 194, 649, 709, 793, 866, 898, 1288
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 6 cut(s) 16, 104, 194, 214, 1091, 1152
HpaII CCGG 5 cut(s) 101, 155, 377, 924, 1272
HphI GGTGA 2 cut(s) 730, 890
Hpy166II GTNNAC 3 cut(s) 701, 729, 1279
Hpy188I TCNGA 8 cut(s) 54, 75, 109, 145, 316, 848, 907, 1252
Hpy188III TCNNGA 9 cut(s) 198, 211, 221, 377, 500, 752, 773, 944, 1000
Hpy8I GTNNAC 3 cut(s) 701, 729, 1279
Hpy99I CGWCG 1 cut(s) 1136
HpyAV CCTTC 1 cut(s) 1131
HpyCH4V TGCA 8 cut(s) 403, 593, 885, 894, 1109, 1220, 1247, 1258
HpyF10VI GCNNNNNNNGC 1 cut(s) 891
HpyF3I CTNAG 6 cut(s) 59, 108, 447, 534, 1227, 1251
Hsp92II CATG 7 cut(s) 194, 649, 709, 793, 866, 898, 1288
Kpn2I TCCGGA 1 cut(s) 376
KpnI GGTACC 1 cut(s) 1039
KroI GCCGGC 1 cut(s) 923
KroNI GCCGGC 1 cut(s) 925
Kzo9I GATC 2 cut(s) 223, 1066
LmnI GCTCC 2 cut(s) 505, 848
Lsp1109I GCAGC 3 cut(s) 50, 267, 974
LweI GCATC 4 cut(s) 894, 949, 1061, 1256
MaeIII GTNAC 4 cut(s) 667, 878, 1027, 1135
MalI GATC 2 cut(s) 225, 1068
MboI GATC 2 cut(s) 223, 1066
MboII GAAGA 3 cut(s) 103, 614, 1070
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 314
MlsI TGGCCA 2 cut(s) 116, 392
MluCI AATT 7 cut(s) 133, 147, 755, 1018, 1055, 1100, 1168
MluNI TGGCCA 2 cut(s) 116, 392
MlyI GAGTC 2 cut(s) 98, 208
Mox20I TGGCCA 2 cut(s) 116, 392
MroI TCCGGA 1 cut(s) 376
MroNI GCCGGC 1 cut(s) 923
MscI TGGCCA 2 cut(s) 116, 392
MseI TTAA 4 cut(s) 333, 510, 822, 1016
MslI CAYNNNNRTG 1 cut(s) 945
Msp20I TGGCCA 2 cut(s) 116, 392
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 5 cut(s) 101, 155, 377, 924, 1272
MspR9I CCNGG 5 cut(s) 101, 118, 155, 828, 1085
MvaI CCWGG 3 cut(s) 118, 828, 1085
MwoI GCNNNNNNNGC 1 cut(s) 891
NaeI GCCGGC 1 cut(s) 925
NciI CCSGG 2 cut(s) 101, 155
NdeII GATC 2 cut(s) 223, 1066
NgoMIV GCCGGC 1 cut(s) 923
NlaIII CATG 7 cut(s) 194, 649, 709, 793, 866, 898, 1288
NlaIV GGNNCC 3 cut(s) 187, 202, 1037
NmeAIII GCCGAG 2 cut(s) 60, 196
NmuCI GTSAC 3 cut(s) 878, 1027, 1135
NspI RCATGY 1 cut(s) 1288
PasI CCCWGGG 1 cut(s) 1084
PdiI GCCGGC 1 cut(s) 925
PfeI GAWTC 4 cut(s) 16, 194, 1091, 1152
PfoI TCCNGGA 1 cut(s) 826
PkrI GCNGC 3 cut(s) 65, 282, 989
PleI GAGTC 2 cut(s) 98, 208
PpsI GAGTC 2 cut(s) 98, 208
PpuMI RGGWCCY 1 cut(s) 1118
Psp5II RGGWCCY 1 cut(s) 1118
Psp6I CCWGG 3 cut(s) 116, 826, 1083
PspGI CCWGG 3 cut(s) 116, 826, 1083
PspN4I GGNNCC 3 cut(s) 187, 202, 1037
PspPI GGNCC 4 cut(s) 98, 200, 921, 1118
PspPPI RGGWCCY 1 cut(s) 1118
PstI CTGCAG 2 cut(s) 1111, 1222
PstNI CAGNNNCTG 2 cut(s) 58, 1226
PsuI RGATCY 1 cut(s) 223
RigI GGCCGGCC 1 cut(s) 927
RsaI GTAC 2 cut(s) 543, 1037
RsaNI GTAC 2 cut(s) 542, 1036
RseI CAYNNNNRTG 1 cut(s) 945
SaqAI TTAA 4 cut(s) 333, 510, 822, 1016
SatI GCNGC 3 cut(s) 64, 281, 988
Sau3AI GATC 2 cut(s) 223, 1066
Sau96I GGNCC 4 cut(s) 98, 200, 921, 1118
SchI GAGTC 2 cut(s) 98, 208
ScrFI CCNGG 5 cut(s) 101, 118, 155, 828, 1085
SduI GDGCHC 1 cut(s) 314
SfaNI GCATC 4 cut(s) 894, 949, 1061, 1256
SfcI CTRYAG 3 cut(s) 672, 1107, 1218
SinI GGWCC 2 cut(s) 200, 1118
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 2 cut(s) 500, 509
SmoI CTYRAG 2 cut(s) 500, 509
Sse9I AATT 7 cut(s) 133, 147, 755, 1018, 1055, 1100, 1168
StyD4I CCNGG 5 cut(s) 99, 116, 153, 826, 1083
StyI CCWWGG 2 cut(s) 393, 1114
TaqI TCGA 3 cut(s) 362, 888, 1069
TasI AATT 7 cut(s) 133, 147, 755, 1018, 1055, 1100, 1168
TatI WGTACW 1 cut(s) 541
TfiI GAWTC 4 cut(s) 16, 194, 1091, 1152
Tru1I TTAA 4 cut(s) 333, 510, 822, 1016
Tru9I TTAA 4 cut(s) 333, 510, 822, 1016
TseFI GTSAC 3 cut(s) 878, 1027, 1135
TseI GCWGC 3 cut(s) 63, 280, 987
Tsp45I GTSAC 3 cut(s) 878, 1027, 1135
TspDTI ATGAA 2 cut(s) 44, 870
Vha464I CTTAAG 1 cut(s) 509
VpaK11BI GGWCC 2 cut(s) 200, 1118
XapI RAATTY 3 cut(s) 1018, 1055, 1100
XceI RCATGY 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.