Rh4AG094000

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001016
Physical Location & Seq
Reverse (-)
19884311 .. 19885796
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG094000.1

Sequence Viewer

Length: 1308 bp
ATGGAGATGCAACAACCAAAGCAGAGGCAGAGGAGTGTTCAGAGGCTAAGATGCTCTGTCATGAACTACGACTGGGGTAAGAAAGGCCGAGACTCTGCGGTGGGCAGGTTGTGTGCCTCCAATTCTCATTCTGAAATCGACCTAGAAAAGCCTTACGCTGAAATGTGGATGGGAACACATGACTCTGGACCCTCCTTTTTGATTGATCAGGATCTGAATAATAATAATAGCACCACTACTGGGTCATCTCCGTCTCTCAGAGACTGGGTTTCTAACAACCCTAATGACATGCTCGGTCACAAGGTTGTCCAAAAATGGGGCTCCTCTGATCTGCCTTTCTTGTTTAAGGTGCAATCCGTGGCAAAGGCATTGTCAATACAGGCACACCCAGATAAGGAATTGGCCAAGGCTTTGCACAAGTCTATGCCAGATATTTACAAGGATGATAATTATAAGCCTGAGATGGCGCTGGCCTTGACACATTTTCAGGCTCTTTGTGGTTTTATCACTCTTGAGGAGCTTAAGAAGGTGATTGGTAATGTCCCTGAGATTGTAGAACTGGTTGGTAGTGAGGTCGAAAACCAAGTGCTTCGCACGACCAAAGAAGATGGGGAGGATAAAGTCAAGTCTGTTATACGTTTGCTTTTTACCCAACTCATGTCAACTAACAAGGAAATTATAACCACAGTAACAACTAAATTGAAGAGTCGTTTACAGACGGAAGGCCAGGTGAGGCAGCTGACAGAAAAGGAGCAGCTAGTGTTGCAATTGGAAAAGCAATATCCAAACGATGTGGGTGTGATATCTGCATTCTTTCTTAACCATGTGAAGCTTAATCCTGGAGAAGCATTATGCCTTGGAGCAAATGAACCCCATGCCTATATATCTGGGGATTGCGTCGAGTGCATGGCAACCTCAGACAACGTAGTTAGGGCTGGCCTTACTCCCAAGCATCGAGATATCAAAACCCTTTGTTCTATGCTCACATATAAGCAGGGTTTCCCTGAAATCTTGCGAGGAGTTGCTGTGAATTCTCATGTAACAAGGTACATACCGCCTTTTGAAGAATTTGAGGTTGATCGGATCCATCTTCCCCAAGGAGAATCAACAGAGTTTCCTGCAGTACCTGGTCCTTCCATTTTTGTGGTCACTTTTGGGAAGGGAACCATATGTACGAGTAATGGAGATCATGCAATCATCCAAGGAGATGTTCTGTTTGCACCTGCACACACTGAGATTAGCATAACAAGTGCATCTGAGTTGCACATATATAGAGCCGGAGTGAATAGCACATTCTTAGCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

435

Amino Acids

48.34

Weight (kDa)

6.08

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 13 - 168 6.3e-44 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 187 - 273 2.1e-14 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 453, 680
AarI CACCTGC 1 cut(s) 1231
Acc36I ACCTGC 2 cut(s) 96, 1231
AciI CCGC 2 cut(s) 98, 1055
AclWI GGATC 3 cut(s) 219, 1078, 1091
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 2 cut(s) 1030, 1067
AfaI GTAC 3 cut(s) 1049, 1125, 1174
AfiI CCNNNNNNNGG 3 cut(s) 240, 316, 394
AflII CTTAAG 1 cut(s) 521
AgsI TTSAA 2 cut(s) 703, 1064
AjnI CCWGG 3 cut(s) 726, 838, 1126
AjuI GAANNNNNNNTTGG 2 cut(s) 1194, 1226
AluBI AGCT 4 cut(s) 520, 739, 757, 832
AluI AGCT 4 cut(s) 520, 739, 757, 832
Alw26I GTCTC 3 cut(s) 84, 255, 258
AlwI GGATC 3 cut(s) 219, 1078, 1091
AlwNI CAGNNNCTG 3 cut(s) 214, 264, 1127
AoxI GGCC 5 cut(s) 85, 402, 471, 724, 937
ApeKI GCWGC 2 cut(s) 736, 754
ApoI RAATTY 2 cut(s) 1030, 1067
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AspLEI GCGC 1 cut(s) 469
AspS9I GGNCC 2 cut(s) 188, 1130
AsuHPI GGTGA 2 cut(s) 541, 742
AvaII GGWCC 2 cut(s) 188, 1130
BalI TGGCCA 1 cut(s) 404
BamHI GGATCC 1 cut(s) 1083
BanII GRGCYC 1 cut(s) 323
BbvI GCAGC 2 cut(s) 748, 766
BccI CCATC 4 cut(s) 163, 457, 602, 1095
BciT130I CCWGG 3 cut(s) 728, 840, 1128
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 3 cut(s) 84, 255, 258
BfaI CTAG 2 cut(s) 143, 758
BfmI CTRYAG 1 cut(s) 1119
BfoI RGCGCY 1 cut(s) 470
BfrI CTTAAG 1 cut(s) 521
BfuAI ACCTGC 2 cut(s) 96, 1231
BisI GCNGC 2 cut(s) 737, 755
BlsI GCNGC 2 cut(s) 738, 756
Bme1390I CCNGG 3 cut(s) 728, 840, 1128
Bme18I GGWCC 2 cut(s) 188, 1130
BmgT120I GGNCC 2 cut(s) 188, 1130
BmiI GGNNCC 4 cut(s) 190, 322, 1085, 1165
BmrFI CCNGG 3 cut(s) 728, 840, 1128
BmrI ACTGGG 3 cut(s) 82, 249, 274
BmsI GCATC 3 cut(s) 41, 961, 1262
BmuI ACTGGG 3 cut(s) 82, 249, 274
BpmI CTGGAG 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 533
BsaBI GATNNNNATC 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 357, 405, 856, 1096, 1201
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 316, 394
Bse1I ACTGG 4 cut(s) 77, 244, 269, 564
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 3 cut(s) 728, 840, 1128
BseDI CCNNGG 5 cut(s) 357, 405, 856, 1096, 1201
BseGI GGATG 3 cut(s) 174, 448, 1197
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 3 cut(s) 240, 316, 394
BseMII CTCAG 6 cut(s) 271, 450, 537, 930, 1224, 1248
BseNI ACTGG 4 cut(s) 77, 244, 269, 564
BseRI GAGGAG 4 cut(s) 46, 313, 530, 1032
BseXI GCAGC 2 cut(s) 748, 766
BsgI GTGCAG 1 cut(s) 1209
BshFI GGCC 5 cut(s) 87, 404, 473, 726, 939
BsiSI CCGG 1 cut(s) 1278
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 3 cut(s) 240, 316, 394
BsmAI GTCTC 3 cut(s) 84, 255, 258
BsmBI CGTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 809
BsnI GGCC 5 cut(s) 87, 404, 473, 726, 939
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
BspACI CCGC 2 cut(s) 98, 1055
BspANI GGCC 5 cut(s) 87, 404, 473, 726, 939
BspCNI CTCAG 6 cut(s) 270, 451, 538, 929, 1225, 1249
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 4 cut(s) 190, 322, 1085, 1165
BspMAI CTGCAG 1 cut(s) 1123
BspMI ACCTGC 2 cut(s) 96, 1231
BspPI GGATC 3 cut(s) 219, 1078, 1091
BspTI CTTAAG 1 cut(s) 521
BsrI ACTGG 4 cut(s) 77, 244, 269, 564
BssECI CCNNGG 5 cut(s) 357, 405, 856, 1096, 1201
BssMI GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
BssT1I CCWWGG 4 cut(s) 405, 856, 1096, 1201
Bst2UI CCWGG 3 cut(s) 728, 840, 1128
Bst4CI ACNGT 1 cut(s) 688
Bst6I CTCTTC 1 cut(s) 698
BstAFI CTTAAG 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 471, 937
BstDEI CTNAG 8 cut(s) 47, 257, 459, 546, 916, 1233, 1257, 1297
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 3 cut(s) 174, 448, 1197
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 1 cut(s) 469
BstKTI GATC 6 cut(s) 208, 214, 331, 1081, 1086, 1189
BstMAI GTCTC 3 cut(s) 84, 255, 258
BstMBI GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
BstMWI GCNNNNNNNGC 2 cut(s) 763, 903
BstNI CCWGG 3 cut(s) 728, 840, 1128
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 726, 838, 1126
BstSFI CTRYAG 1 cut(s) 1119
BstV1I GCAGC 2 cut(s) 748, 766
BstX2I RGATCY 2 cut(s) 211, 1083
BstXI CCANNNNNNTGG 1 cut(s) 1144
BstYI RGATCY 2 cut(s) 211, 1083
BsuRI GGCC 5 cut(s) 87, 404, 473, 726, 939
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 174, 448, 1197
BtsIMutI CAGTG 1 cut(s) 1230
BveI ACCTGC 2 cut(s) 96, 1231
Cac8I GCNNGC 2 cut(s) 471, 937
CaiI CAGNNNCTG 3 cut(s) 214, 264, 1127
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 469
Cfr13I GGNCC 2 cut(s) 188, 1130
CseI GACGC 1 cut(s) 886
CsiI ACCWGGT 1 cut(s) 1126
Csp6I GTAC 3 cut(s) 1048, 1124, 1173
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviAII CATG 9 cut(s) 61, 179, 289, 658, 824, 875, 907, 1037, 1190
CviQI GTAC 3 cut(s) 1048, 1124, 1173
DdeI CTNAG 8 cut(s) 47, 257, 459, 546, 916, 1233, 1257, 1297
DpnI GATC 6 cut(s) 207, 213, 330, 1080, 1085, 1188
DpnII GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 698
Eco130I CCWWGG 4 cut(s) 405, 856, 1096, 1201
Eco24I GRGCYC 1 cut(s) 323
Eco32I GATATC 2 cut(s) 804, 961
Eco47I GGWCC 2 cut(s) 188, 1130
EcoRI GAATTC 1 cut(s) 1030
EcoRII CCWGG 3 cut(s) 726, 838, 1126
EcoRV GATATC 2 cut(s) 804, 961
EcoT14I CCWWGG 4 cut(s) 405, 856, 1096, 1201
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 4 cut(s) 405, 856, 1096, 1201
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 9 cut(s) 64, 182, 292, 661, 827, 878, 910, 1040, 1193
FaqI GGGAC 1 cut(s) 527
FatI CATG 9 cut(s) 60, 178, 288, 657, 823, 874, 906, 1036, 1189
FauNDI CATATG 1 cut(s) 1169
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 737, 755
FokI GGATG 3 cut(s) 181, 455, 1184
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 737, 755
FspBI CTAG 2 cut(s) 143, 758
GlaI GCGC 1 cut(s) 468
GluI GCNGC 2 cut(s) 737, 755
GsuI CTGGAG 1 cut(s) 861
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 5 cut(s) 87, 404, 473, 726, 939
HapII CCGG 1 cut(s) 1278
HgaI GACGC 1 cut(s) 886
HhaI GCGC 1 cut(s) 469
Hin1II CATG 9 cut(s) 64, 182, 292, 661, 827, 878, 910, 1040, 1193
Hin6I GCGC 1 cut(s) 467
HinP1I GCGC 1 cut(s) 467
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 4 cut(s) 92, 182, 706, 1103
HpaII CCGG 1 cut(s) 1278
HphI GGTGA 2 cut(s) 541, 742
Hpy166II GTNNAC 2 cut(s) 663, 713
Hpy188I TCNGA 8 cut(s) 42, 133, 216, 260, 328, 919, 1083, 1258
Hpy188III TCNNGA 5 cut(s) 61, 186, 209, 512, 956
Hpy8I GTNNAC 2 cut(s) 663, 713
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 4 cut(s) 520, 716, 1143, 1153
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4IV ACGT 2 cut(s) 637, 924
HpyF10VI GCNNNNNNNGC 2 cut(s) 763, 903
HpyF3I CTNAG 8 cut(s) 47, 257, 459, 546, 916, 1233, 1257, 1297
HpySE526I ACGT 2 cut(s) 637, 924
Hsp92II CATG 9 cut(s) 64, 182, 292, 661, 827, 878, 910, 1040, 1193
HspAI GCGC 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
LmnI GCTCC 4 cut(s) 326, 517, 751, 860
Lsp1109I GCAGC 2 cut(s) 748, 766
LweI GCATC 3 cut(s) 41, 961, 1262
MabI ACCWGGT 1 cut(s) 1126
MaeI CTAG 2 cut(s) 143, 758
MaeII ACGT 2 cut(s) 637, 924
MaeIII GTNAC 4 cut(s) 296, 688, 1039, 1147
MalI GATC 6 cut(s) 207, 213, 330, 1080, 1085, 1188
MboI GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
MboII GAAGA 4 cut(s) 617, 715, 1076, 1082
MfeI CAATTG 1 cut(s) 767
MflI RGATCY 2 cut(s) 211, 1083
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 8 cut(s) 121, 398, 448, 675, 698, 767, 1030, 1067
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 3 cut(s) 86, 176, 715
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
MseI TTAA 4 cut(s) 345, 522, 819, 834
MslI CAYNNNNRTG 1 cut(s) 1142
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 739
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 1 cut(s) 1278
MspR9I CCNGG 3 cut(s) 728, 840, 1128
MunI CAATTG 1 cut(s) 767
Mva1269I GAATGC 1 cut(s) 809
MvaI CCWGG 3 cut(s) 728, 840, 1128
MwoI GCNNNNNNNGC 2 cut(s) 763, 903
NdeI CATATG 1 cut(s) 1169
NdeII GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
NlaIII CATG 9 cut(s) 64, 182, 292, 661, 827, 878, 910, 1040, 1193
NlaIV GGNNCC 4 cut(s) 190, 322, 1085, 1165
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 2 cut(s) 296, 1147
NspI RCATGY 1 cut(s) 292
PagI TCATGA 1 cut(s) 60
PaqCI CACCTGC 1 cut(s) 1231
PctI GAATGC 1 cut(s) 809
PfeI GAWTC 1 cut(s) 1103
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 2 cut(s) 738, 756
PleI GAGTC 3 cut(s) 86, 176, 714
PpsI GAGTC 3 cut(s) 86, 176, 714
PsiI TTATAA 2 cut(s) 453, 680
Psp6I CCWGG 3 cut(s) 726, 838, 1126
PspGI CCWGG 3 cut(s) 726, 838, 1126
PspN4I GGNNCC 4 cut(s) 190, 322, 1085, 1165
PspPI GGNCC 2 cut(s) 188, 1130
PsrI GAACNNNNNNTAC 2 cut(s) 1156, 1188
PstI CTGCAG 1 cut(s) 1123
PstNI CAGNNNCTG 3 cut(s) 214, 264, 1127
PsuI RGATCY 2 cut(s) 211, 1083
PvuII CAGCTG 1 cut(s) 739
RsaI GTAC 3 cut(s) 1049, 1125, 1174
RsaNI GTAC 3 cut(s) 1048, 1124, 1173
RseI CAYNNNNRTG 1 cut(s) 1142
SaqAI TTAA 4 cut(s) 345, 522, 819, 834
SatI GCNGC 2 cut(s) 737, 755
Sau3AI GATC 6 cut(s) 205, 211, 328, 1078, 1083, 1186
Sau96I GGNCC 2 cut(s) 188, 1130
SchI GAGTC 3 cut(s) 86, 176, 715
ScrFI CCNGG 3 cut(s) 728, 840, 1128
SduI GDGCHC 1 cut(s) 323
SexAI ACCWGGT 1 cut(s) 1126
SfaNI GCATC 3 cut(s) 41, 961, 1262
SfcI CTRYAG 1 cut(s) 1119
SinI GGWCC 2 cut(s) 188, 1130
SmiMI CAYNNNNRTG 1 cut(s) 1142
SmlI CTYRAG 2 cut(s) 512, 521
SmoI CTYRAG 2 cut(s) 512, 521
Sse9I AATT 8 cut(s) 121, 398, 448, 675, 698, 767, 1030, 1067
SsiI CCGC 2 cut(s) 98, 1055
SspMI CTAG 2 cut(s) 143, 758
StyD4I CCNGG 3 cut(s) 726, 838, 1126
StyI CCWWGG 4 cut(s) 405, 856, 1096, 1201
TaaI ACNGT 1 cut(s) 688
TaiI ACGT 2 cut(s) 640, 927
TaqI TCGA 4 cut(s) 138, 576, 900, 955
TaqII GACCGA 1 cut(s) 284
TasI AATT 8 cut(s) 121, 398, 448, 675, 698, 767, 1030, 1067
TfiI GAWTC 1 cut(s) 1103
Tru1I TTAA 4 cut(s) 345, 522, 819, 834
Tru9I TTAA 4 cut(s) 345, 522, 819, 834
TscAI CASTG 1 cut(s) 1237
TseFI GTSAC 2 cut(s) 296, 1147
TseI GCWGC 2 cut(s) 736, 754
Tsp45I GTSAC 2 cut(s) 296, 1147
TspDTI ATGAA 2 cut(s) 77, 882
TspGWI ACGGA 3 cut(s) 240, 346, 734
TspRI CASTG 1 cut(s) 1237
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 188, 1130
XapI RAATTY 2 cut(s) 1030, 1067
XceI RCATGY 1 cut(s) 292
XspI CTAG 2 cut(s) 143, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.