Prupe.1G313500_v2.0.a1

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001016
Physical Location & Seq
Reverse (-)
30358305 .. 30362021
3717 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G313500.1

Sequence Viewer

Length: 1317 bp
ATGGAGCCCTGGGACAAGAACATGAAGAAGAAGAGGAAGCGGAGCCGGAGGCCCGTCAGGCTCAGATGCTCCGTCAAGAATTACGATTGGGGCATTGTCGGCCGCCATTCCAAGGTCGCCAGGCTTTTCGCGCTCAATTCAGGGTCGGATATCGACCCCGCCAAGCCCTACGCCGAGTTCTGGATCGGGACGCATGAATCCGGGCCGTCGTATGCCGATTCCGGGTCGGAGCCCGTGAGCCTCAAGGCCTGGATCGCTCAGGATCCCAGTGTGCTTGGGGACAAGGTTGTTGACAAGTGGGGTGCTGATCTTCCCTTCTTGTTCAAGGTACTTTCGGTTGGGAAGGCTTTATCAATACAGGCTCATCCGGATAAAGAACTAGCTAGGGTGCTGCACAAGTTATACCCAAATGTTTACAGAGATGACAATCACAAGCCTGAAATGGCATTGGCATTGACAGAGTTTGAGGCTCTTTGCGGGTTTATTAGTGTCAAGGAGCTAAAAGACATGCTTAGTAGTGTTCCCGAGATTGCAGAGTTGGTTGGGGTTGGAAATGCAGAGAGAATTTTACTTGTTAATGAGCAGCATGAGAATGGGAAATCAAAAACGGATTTAGAGTCCATCTTCAGTCGAGTTATGTTACTAAGTAAAGATACAATTTCTGAGATGATATCTAAACTGAAAAGGCGTCTTAATCTAGAGAAAAAGAAAAGGCAATTGACGGTTAAAGAACAACTGGCACTCAGATTGGAGAGTCAATATCCAGCTGATGTGGGTGCAATAGCAGCATTTTTTCTTAACTATGTGAAGCTGAACCGTGGTGAAGCAATAAGTCTTGGGCCAAATGAGCCCCATGCATATATTTCTGGTGAATGCATTGAGTGTATGGCAACTTCTGACAATGTTGTTCGTGCCGGCCTTACCTCAAAACCACTGGATGTCCAAACGCTTCTTTCTATGCTTAAATACAGACAGGGTTCTCCTGAGATTTTGCAAGGGGTTCCTTTGAATCCATACACAACAAGATATCTCCCACCTTTCGAAGAATTCGAAATTGATTGCTGTAATCTTCCTCAGTCTGCAGCGGTAATTTTTCCCTCAGTCGTAGGACCATCCCTTTTCCTATTCACTGCTGGGAAAGGACATTTTGATGCAGGTCTGCCCGAGGATGATATGGTGAAGGAGGGGGATGATATAGTCGAAGAGGGTGAGGTTTTCTTTGTGCCTGCCAACACGAAGATTATCATTACTGCCAAATCAACAGAACTGCAACTGTATAGGGTGGGGGTGAACAGCAAAATTTATAGGGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

439

Amino Acids

48.93

Weight (kDa)

6.74

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1145
AccII CGCG 1 cut(s) 131
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 5 cut(s) 40, 103, 159, 477, 1085
AclWI GGATC 4 cut(s) 191, 257, 260, 270
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 3 cut(s) 564, 1046, 1299
AcuI CTGAAG 1 cut(s) 610
AcyI GRCGYC 1 cut(s) 688
AfaI GTAC 1 cut(s) 330
AfiI CCNNNNNNNGG 3 cut(s) 112, 180, 222
AgsI TTSAA 2 cut(s) 325, 1009
AjnI CCWGG 3 cut(s) 8, 119, 248
AluBI AGCT 4 cut(s) 383, 499, 767, 811
AluI AGCT 4 cut(s) 383, 499, 767, 811
AlwI GGATC 4 cut(s) 191, 257, 260, 270
Ama87I CYCGRG 2 cut(s) 524, 1163
Aor13HI TCCGGA 1 cut(s) 367
AoxI GGCC 6 cut(s) 50, 100, 203, 246, 839, 916
ApeKI GCWGC 4 cut(s) 391, 583, 785, 1082
ApoI RAATTY 3 cut(s) 564, 1046, 1299
AspLEI GCGC 1 cut(s) 133
AspS9I GGNCC 4 cut(s) 51, 203, 839, 1109
AsuC2I CCSGG 2 cut(s) 202, 223
AsuHPI GGTGA 5 cut(s) 833, 881, 1189, 1220, 1300
AsuII TTCGAA 2 cut(s) 1041, 1050
AvaI CYCGRG 2 cut(s) 524, 1163
AvaII GGWCC 1 cut(s) 1109
BamHI GGATCC 1 cut(s) 262
BanII GRGCYC 3 cut(s) 9, 234, 852
BarI GAAGNNNNNNTAC 2 cut(s) 877, 909
BbvI GCAGC 4 cut(s) 378, 595, 797, 1094
BccI CCATC 2 cut(s) 629, 1120
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 3 cut(s) 10, 121, 250
BcnI CCSGG 2 cut(s) 202, 223
BfaI CTAG 3 cut(s) 380, 384, 698
BfmI CTRYAG 1 cut(s) 1080
BfuAI ACCTGC 1 cut(s) 1145
BglI GCCNNNNNGGC 1 cut(s) 58
BisI GCNGC 5 cut(s) 103, 392, 584, 786, 1083
BlsI GCNGC 5 cut(s) 104, 393, 585, 787, 1084
Bme1390I CCNGG 5 cut(s) 10, 121, 202, 223, 250
Bme18I GGWCC 1 cut(s) 1109
BmeT110I CYCGRG 2 cut(s) 524, 1163
BmgT120I GGNCC 4 cut(s) 51, 203, 839, 1109
BmiI GGNNCC 5 cut(s) 6, 44, 231, 264, 1002
BmrFI CCNGG 5 cut(s) 10, 121, 202, 223, 250
BmrI ACTGGG 1 cut(s) 261
BmsI GCATC 2 cut(s) 56, 1141
BmuI ACTGGG 1 cut(s) 261
Bpu10I CCTNAGC 1 cut(s) 258
Bpu14I TTCGAA 2 cut(s) 1041, 1050
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 2 cut(s) 202, 223
BsaBI GATNNNNATC 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 688
BsaJI CCNNGG 5 cut(s) 8, 9, 111, 817, 1164
BsaWI WCCGGW 1 cut(s) 367
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 180, 222
Bse118I RCCGGY 1 cut(s) 914
Bse1I ACTGG 3 cut(s) 267, 741, 939
Bse8I GATNNNNATC 1 cut(s) 426
BseAI TCCGGA 1 cut(s) 367
BseBI CCWGG 3 cut(s) 10, 121, 250
BseDI CCNNGG 5 cut(s) 8, 9, 111, 817, 1164
BseGI GGATG 5 cut(s) 364, 943, 1112, 1174, 1195
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 3 cut(s) 112, 180, 222
BseMII CTCAG 7 cut(s) 76, 272, 654, 757, 975, 1088, 1113
BseNI ACTGG 3 cut(s) 267, 741, 939
BseX3I CGGCCG 1 cut(s) 100
BseXI GCAGC 4 cut(s) 378, 595, 797, 1094
BseYI CCCAGC 1 cut(s) 1133
BsgI GTGCAG 1 cut(s) 377
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 6 cut(s) 52, 102, 205, 248, 841, 918
BsiEI CGRYCG 1 cut(s) 103
BsiHKCI CYCGRG 2 cut(s) 524, 1163
BsiSI CCGG 5 cut(s) 46, 201, 222, 368, 915
BslFI GGGAC 3 cut(s) 26, 202, 293
BslI CCNNNNNNNGG 3 cut(s) 112, 180, 222
BsmFI GGGAC 3 cut(s) 26, 202, 293
BsmI GAATGC 1 cut(s) 878
BsnI GGCC 6 cut(s) 52, 102, 205, 248, 841, 918
BsoBI CYCGRG 2 cut(s) 524, 1163
Bsp119I TTCGAA 2 cut(s) 1041, 1050
Bsp1286I GDGCHC 3 cut(s) 9, 234, 852
Bsp13I TCCGGA 1 cut(s) 367
Bsp143I GATC 4 cut(s) 183, 252, 262, 307
BspACI CCGC 5 cut(s) 40, 103, 159, 477, 1085
BspANI GGCC 6 cut(s) 52, 102, 205, 248, 841, 918
BspCNI CTCAG 7 cut(s) 75, 271, 655, 756, 976, 1087, 1112
BspEI TCCGGA 1 cut(s) 367
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 5 cut(s) 6, 44, 231, 264, 1002
BspMAI CTGCAG 1 cut(s) 1084
BspMI ACCTGC 1 cut(s) 1145
BspPI GGATC 4 cut(s) 191, 257, 260, 270
BspT104I TTCGAA 2 cut(s) 1041, 1050
BsrFI RCCGGY 1 cut(s) 914
BsrI ACTGG 3 cut(s) 267, 741, 939
BssAI RCCGGY 1 cut(s) 914
BssECI CCNNGG 5 cut(s) 8, 9, 111, 817, 1164
BssMI GATC 4 cut(s) 183, 252, 262, 307
BssNI GRCGYC 1 cut(s) 688
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 3 cut(s) 10, 121, 250
Bst4CI ACNGT 3 cut(s) 724, 818, 1275
Bst6I CTCTTC 2 cut(s) 26, 1197
BstACI GRCGYC 1 cut(s) 688
BstBI TTCGAA 2 cut(s) 1041, 1050
BstC8I GCNNGC 2 cut(s) 916, 1227
BstDEI CTNAG 9 cut(s) 62, 258, 512, 644, 663, 743, 984, 1074, 1099
BstDSI CCRYGG 1 cut(s) 817
BstF5I GGATG 5 cut(s) 364, 943, 1112, 1174, 1195
BstFNI CGCG 1 cut(s) 131
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 4 cut(s) 186, 255, 265, 310
BstMBI GATC 4 cut(s) 183, 252, 262, 307
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 6 cut(s) 58, 99, 130, 254, 785, 847
BstNI CCWGG 3 cut(s) 10, 121, 250
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 5 cut(s) 8, 119, 200, 221, 248
BstSFI CTRYAG 1 cut(s) 1080
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 4 cut(s) 378, 595, 797, 1094
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BstZI CGGCCG 1 cut(s) 100
BsuRI GGCC 6 cut(s) 52, 102, 205, 248, 841, 918
BtgI CCRYGG 1 cut(s) 817
BtsCI GGATG 5 cut(s) 364, 943, 1112, 1174, 1195
BtsI GCAGTG 1 cut(s) 1128
BtsIMutI CAGTG 3 cut(s) 274, 932, 1128
BveI ACCTGC 1 cut(s) 1145
Cac8I GCNNGC 2 cut(s) 916, 1227
CfoI GCGC 1 cut(s) 133
Cfr10I RCCGGY 1 cut(s) 914
Cfr13I GGNCC 4 cut(s) 51, 203, 839, 1109
CseI GACGC 2 cut(s) 199, 677
Csp6I GTAC 1 cut(s) 329
CviAII CATG 5 cut(s) 22, 194, 508, 587, 854
CviQI GTAC 1 cut(s) 329
DdeI CTNAG 9 cut(s) 62, 258, 512, 644, 663, 743, 984, 1074, 1099
DpnI GATC 4 cut(s) 185, 254, 264, 309
DpnII GATC 4 cut(s) 183, 252, 262, 307
EaeI YGGCCR 1 cut(s) 100
EagI CGGCCG 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 26, 1197
EarI CTCTTC 2 cut(s) 26, 1197
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 248
Eco24I GRGCYC 3 cut(s) 9, 234, 852
Eco32I GATATC 3 cut(s) 151, 672, 1028
Eco47I GGWCC 1 cut(s) 1109
Eco52I CGGCCG 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 610
Eco88I CYCGRG 2 cut(s) 524, 1163
EcoRI GAATTC 1 cut(s) 1046
EcoRII CCWGG 3 cut(s) 8, 119, 248
EcoRV GATATC 3 cut(s) 151, 672, 1028
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 2 cut(s) 859, 878
EcoT38I GRGCYC 3 cut(s) 9, 234, 852
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 5 cut(s) 25, 197, 511, 590, 857
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 3 cut(s) 26, 202, 293
FatI CATG 5 cut(s) 21, 193, 507, 586, 853
FauI CCCGC 2 cut(s) 166, 470
Fnu4HI GCNGC 5 cut(s) 103, 392, 584, 786, 1083
FokI GGATG 5 cut(s) 351, 950, 1099, 1181, 1202
FriOI GRGCYC 3 cut(s) 9, 234, 852
Fsp4HI GCNGC 5 cut(s) 103, 392, 584, 786, 1083
FspBI CTAG 3 cut(s) 380, 384, 698
GlaI GCGC 1 cut(s) 132
GluI GCNGC 5 cut(s) 103, 392, 584, 786, 1083
GsaI CCCAGC 1 cut(s) 1137
HaeIII GGCC 6 cut(s) 52, 102, 205, 248, 841, 918
HapII CCGG 5 cut(s) 46, 201, 222, 368, 915
HgaI GACGC 2 cut(s) 199, 677
HhaI GCGC 1 cut(s) 133
Hin1I GRCGYC 1 cut(s) 688
Hin1II CATG 5 cut(s) 25, 197, 511, 590, 857
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 5 cut(s) 197, 218, 617, 754, 1009
HpaII CCGG 5 cut(s) 46, 201, 222, 368, 915
HphI GGTGA 5 cut(s) 833, 881, 1189, 1220, 1300
Hpy166II GTNNAC 3 cut(s) 292, 415, 1291
Hpy188I TCNGA 6 cut(s) 65, 148, 229, 664, 746, 898
Hpy188III TCNNGA 8 cut(s) 76, 181, 187, 260, 368, 524, 698, 983
Hpy8I GTNNAC 3 cut(s) 292, 415, 1291
Hpy99I CGWCG 1 cut(s) 211
HpyAV CCTTC 3 cut(s) 325, 337, 1174
HpyCH4III ACNGT 3 cut(s) 724, 818, 1275
HpyF10VI GCNNNNNNNGC 6 cut(s) 58, 99, 130, 254, 785, 847
HpyF3I CTNAG 9 cut(s) 62, 258, 512, 644, 663, 743, 984, 1074, 1099
Hsp92I GRCGYC 1 cut(s) 688
Hsp92II CATG 5 cut(s) 25, 197, 511, 590, 857
HspAI GCGC 1 cut(s) 131
Kpn2I TCCGGA 1 cut(s) 367
KroI GCCGGC 1 cut(s) 914
KroNI GCCGGC 1 cut(s) 916
Kzo9I GATC 4 cut(s) 183, 252, 262, 307
LmnI GCTCC 5 cut(s) 4, 42, 74, 229, 496
Lsp1109I GCAGC 4 cut(s) 378, 595, 797, 1094
LweI GCATC 2 cut(s) 56, 1141
MaeI CTAG 3 cut(s) 380, 384, 698
MaeIII GTNAC 1 cut(s) 639
MalI GATC 4 cut(s) 185, 254, 264, 309
MboI GATC 4 cut(s) 183, 252, 262, 307
MboII GAAGA 9 cut(s) 37, 40, 43, 302, 616, 1055, 1061, 1214, 1249
MfeI CAATTG 1 cut(s) 716
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 3 cut(s) 9, 234, 852
MluCI AATT 9 cut(s) 79, 136, 564, 657, 716, 1046, 1053, 1089, 1299
MlyI GAGTC 2 cut(s) 626, 763
MmeI TCCRAC 3 cut(s) 126, 207, 529
Mph1103I ATGCAT 2 cut(s) 859, 878
MroI TCCGGA 1 cut(s) 367
MroNI GCCGGC 1 cut(s) 914
MseI TTAA 5 cut(s) 576, 693, 726, 798, 963
MslI CAYNNNNRTG 2 cut(s) 591, 1149
MspA1I CMGCKG 2 cut(s) 767, 1085
MspI CCGG 5 cut(s) 46, 201, 222, 368, 915
MspR9I CCNGG 5 cut(s) 10, 121, 202, 223, 250
MunI CAATTG 1 cut(s) 716
Mva1269I GAATGC 1 cut(s) 878
MvaI CCWGG 3 cut(s) 10, 121, 250
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 6 cut(s) 58, 99, 130, 254, 785, 847
NaeI GCCGGC 1 cut(s) 916
NciI CCSGG 2 cut(s) 202, 223
NdeII GATC 4 cut(s) 183, 252, 262, 307
NgoMIV GCCGGC 1 cut(s) 914
NlaIII CATG 5 cut(s) 25, 197, 511, 590, 857
NlaIV GGNNCC 5 cut(s) 6, 44, 231, 264, 1002
NmeAIII GCCGAG 1 cut(s) 199
NsiI ATGCAT 2 cut(s) 859, 878
NspI RCATGY 1 cut(s) 511
NspV TTCGAA 2 cut(s) 1041, 1050
PasI CCCWGGG 1 cut(s) 9
PceI AGGCCT 1 cut(s) 248
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PctI GAATGC 1 cut(s) 878
PdiI GCCGGC 1 cut(s) 916
PfeI GAWTC 3 cut(s) 197, 218, 1009
PkrI GCNGC 5 cut(s) 104, 393, 585, 787, 1084
PleI GAGTC 2 cut(s) 625, 762
PpsI GAGTC 2 cut(s) 625, 762
Psp6I CCWGG 3 cut(s) 8, 119, 248
PspFI CCCAGC 1 cut(s) 1133
PspGI CCWGG 3 cut(s) 8, 119, 248
PspN4I GGNNCC 5 cut(s) 6, 44, 231, 264, 1002
PspPI GGNCC 4 cut(s) 51, 203, 839, 1109
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
RseI CAYNNNNRTG 2 cut(s) 591, 1149
SaqAI TTAA 5 cut(s) 576, 693, 726, 798, 963
SatI GCNGC 5 cut(s) 103, 392, 584, 786, 1083
Sau3AI GATC 4 cut(s) 183, 252, 262, 307
Sau96I GGNCC 4 cut(s) 51, 203, 839, 1109
SchI GAGTC 2 cut(s) 626, 763
ScrFI CCNGG 5 cut(s) 10, 121, 202, 223, 250
SduI GDGCHC 3 cut(s) 9, 234, 852
SfaNI GCATC 2 cut(s) 56, 1141
SfcI CTRYAG 1 cut(s) 1080
SfuI TTCGAA 2 cut(s) 1041, 1050
SinI GGWCC 1 cut(s) 1109
SmiMI CAYNNNNRTG 2 cut(s) 591, 1149
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 9 cut(s) 79, 136, 564, 657, 716, 1046, 1053, 1089, 1299
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 5 cut(s) 40, 103, 159, 477, 1085
SspMI CTAG 3 cut(s) 380, 384, 698
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 5 cut(s) 8, 119, 200, 221, 248
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 3 cut(s) 724, 818, 1275
TaqI TCGA 5 cut(s) 153, 631, 1041, 1050, 1200
TasI AATT 9 cut(s) 79, 136, 564, 657, 716, 1046, 1053, 1089, 1299
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 3 cut(s) 197, 218, 1009
Tru1I TTAA 5 cut(s) 576, 693, 726, 798, 963
Tru9I TTAA 5 cut(s) 576, 693, 726, 798, 963
TscAI CASTG 3 cut(s) 274, 939, 1135
TseI GCWGC 4 cut(s) 391, 583, 785, 1082
TspDTI ATGAA 2 cut(s) 38, 210
TspGWI ACGGA 2 cut(s) 61, 623
TspRI CASTG 3 cut(s) 274, 939, 1135
VpaK11BI GGWCC 1 cut(s) 1109
XapI RAATTY 3 cut(s) 564, 1046, 1299
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 1 cut(s) 511
XspI CTAG 3 cut(s) 380, 384, 698
Zsp2I ATGCAT 2 cut(s) 859, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.