Rw5G048990

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001016
Physical Location & Seq
Forward (+)
85007321 .. 85014190
6870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048990.1

Sequence Viewer

Length: 1245 bp
ATGGAGAAGCAGATTCTACAGAGGCTGAGATGCTCGGTCCAGAATTACGACTGGGGTAAGAAAGGCGGAGACTCTGAGGTGGGCAGGCTCTATGCCTACAACTCTGGTTCTGAAATTGACCCAGAAAAGCCTTACGCAGAGTTTTGGATGGGAACCCATGACTCTGGACCATCCTTTTTGATTGATAAGGATCTGAATATTAACACGAGGAATGAATCGTTTTTGCTTCTCAAAGACTGGGTCGTCGAGAAGTGGGGCTCTGACCTGCCTTTCTTATTTAAGGTGCTTTCGGTGGCAAAGGCTTTGTCAATCCAGGCACATCCAGATCAGGACTTGGCCAAGGCTTTGCACAAGCTGATGCCAAACTTTTACAAGGACGACAATCACAAGCCTGAGATGGCGCTTGCGCTAACACATTTTCAGGCTCTTTGTGGTTTTATTACTGTTGAGGAGCTAAAGGGAGTGCTTCATAGTGTCCCAGAGATTGCACAAGTGGTGGGTAGTGAAGTTGCAAGCCAAGTGCTTCACAGTACTGCTGAAGATGGGGAGGATAAAGTCAAGTCTGTTCTACGTTCAATTTTCACCCAACTTATGTTAGCTAATAAAGAGATGATAAGTACAGTAACAACCACATTGAAAAGTCGTCTACTTATGGAAAGTCAGACGAGGCAGTTGACAGAGAAGGAAGAGCTGGTGTTGCAATTGGAAAAGCAATATCCAGATGATGTGGGCGTTATATCTGCCTTCTTTCTCAACCATGTCAACCTTAATCCTGGAGAAGCACTATACCTTGGAGCAAATGAACCCCATGCCTATTTATCCGGCGACTGCATCGAGTGCATGGCAAGCTCGGACAACGTAGTGAGGGCCGGCCTTACTCCCAAGCATCGGGATATTAAAACCCTTTGTTCTATGCTCACATACAAACAGGGCTTTCCTGAAATCCTACGAGGAGATTCTATAAATCCTTATGTAACAAGGTACGTCCCACCTTTTGAAGAATTTGAGGTTGATCGATGTCATCTTCCTGAAGGAAAATCCACAGAATTTTCTGCAAGCCCAGGTCCTTCCCTTTTTGTGGTAACTTTTGGAGTGGGAGCAATATATACAGGTGGTACAAAATATGGTATCACCCGAGGAGACGTTCTTTTTGCACCCGCTGACACTGAGATGAGCATAAGAAGTGAATCTGAGTTGCAGATATATAGAGCCGGAGTGAACAGCAAGTTCCTTCGAGCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

414

Amino Acids

46.2

Weight (kDa)

5.39

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 6 - 146 6.9e-49 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 166 - 251 1.2e-14 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 273
AccI GTMKAC 1 cut(s) 646
AciI CCGC 2 cut(s) 66, 1158
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 2 cut(s) 1001, 1046
AcuI CTGAAG 2 cut(s) 558, 1050
AfaI GTAC 4 cut(s) 532, 619, 983, 1117
AfiI CCNNNNNNNGG 2 cut(s) 888, 1078
AgsI TTSAA 3 cut(s) 576, 637, 998
AjnI CCWGG 3 cut(s) 312, 772, 1060
AluBI AGCT 5 cut(s) 355, 454, 599, 691, 849
AluI AGCT 5 cut(s) 355, 454, 599, 691, 849
Alw26I GTCTC 2 cut(s) 63, 1134
AlwI GGATC 1 cut(s) 198
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 1134
AoxI GGCC 3 cut(s) 336, 867, 871
ApoI RAATTY 2 cut(s) 1001, 1046
ArsI GACNNNNNNTTYG 6 cut(s) 225, 257, 290, 322, 1133, 1165
AspLEI GCGC 2 cut(s) 403, 409
AspS9I GGNCC 4 cut(s) 37, 167, 867, 1064
AsuHPI GGTGA 2 cut(s) 574, 1123
AvaI CYCGRG 1 cut(s) 1134
AvaII GGWCC 3 cut(s) 37, 167, 1064
BalI TGGCCA 1 cut(s) 338
BanII GRGCYC 1 cut(s) 260
BauI CACGAG 1 cut(s) 205
BccI CCATC 4 cut(s) 142, 178, 391, 536
BciT130I CCWGG 3 cut(s) 314, 774, 1062
BcoDI GTCTC 2 cut(s) 63, 1134
BfmI CTRYAG 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 273
BmcAI AGTACT 1 cut(s) 532
Bme1390I CCNGG 3 cut(s) 314, 774, 1062
Bme18I GGWCC 3 cut(s) 37, 167, 1064
BmeT110I CYCGRG 1 cut(s) 1134
BmgT120I GGNCC 4 cut(s) 37, 167, 867, 1064
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 3 cut(s) 314, 774, 1062
BmrI ACTGGG 2 cut(s) 61, 247
BmsI GCATC 4 cut(s) 20, 348, 840, 895
BmuI ACTGGG 2 cut(s) 61, 247
BpmI CTGGAG 1 cut(s) 795
Bsa29I ATCGAT 1 cut(s) 1015
BsaBI GATNNNNATC 1 cut(s) 189
BsaJI CCNNGG 4 cut(s) 339, 790, 1060, 1135
Bsc4I CCNNNNNNNGG 2 cut(s) 888, 1078
Bse118I RCCGGY 1 cut(s) 869
Bse1I ACTGG 2 cut(s) 56, 242
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 3 cut(s) 314, 774, 1062
BseCI ATCGAT 1 cut(s) 1015
BseDI CCNNGG 4 cut(s) 339, 790, 1060, 1135
BseGI GGATG 3 cut(s) 153, 170, 319
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 888, 1078
BseMII CTCAG 5 cut(s) 17, 66, 384, 1158, 1182
BseNI ACTGG 2 cut(s) 56, 242
BseRI GAGGAG 3 cut(s) 464, 966, 1152
BshFI GGCC 3 cut(s) 338, 869, 873
BshVI ATCGAT 1 cut(s) 1015
BsiHKCI CYCGRG 1 cut(s) 1134
BsiSI CCGG 3 cut(s) 822, 870, 1212
BslFI GGGAC 2 cut(s) 461, 971
BslI CCNNNNNNNGG 2 cut(s) 888, 1078
BsmAI GTCTC 2 cut(s) 63, 1134
BsmBI CGTCTC 1 cut(s) 1134
BsmFI GGGAC 2 cut(s) 461, 971
BsnI GGCC 3 cut(s) 338, 869, 873
BsoBI CYCGRG 1 cut(s) 1134
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 3 cut(s) 190, 325, 1012
BspACI CCGC 2 cut(s) 66, 1158
BspANI GGCC 3 cut(s) 338, 869, 873
BspCNI CTCAG 5 cut(s) 18, 67, 385, 1159, 1183
BspDI ATCGAT 1 cut(s) 1015
BspLI GGNNCC 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 273
BspPI GGATC 1 cut(s) 198
BspQI GCTCTTC 1 cut(s) 681
BsrFI RCCGGY 1 cut(s) 869
BsrI ACTGG 2 cut(s) 56, 242
BssAI RCCGGY 1 cut(s) 869
BssECI CCNNGG 4 cut(s) 339, 790, 1060, 1135
BssMI GATC 3 cut(s) 190, 325, 1012
BssSI CACGAG 1 cut(s) 205
BssT1I CCWWGG 2 cut(s) 339, 790
Bst2BI CACGAG 1 cut(s) 205
Bst2UI CCWGG 3 cut(s) 314, 774, 1062
Bst4CI ACNGT 3 cut(s) 445, 530, 622
Bst6I CTCTTC 1 cut(s) 681
BstAPI GCANNNNNTGC 1 cut(s) 837
BstC8I GCNNGC 6 cut(s) 86, 405, 514, 847, 871, 1057
BstDEI CTNAG 5 cut(s) 26, 75, 393, 1167, 1191
BstF5I GGATG 3 cut(s) 153, 170, 319
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 2 cut(s) 403, 409
BstKTI GATC 3 cut(s) 193, 328, 1015
BstMAI GTCTC 2 cut(s) 63, 1134
BstMBI GATC 3 cut(s) 190, 325, 1012
BstMWI GCNNNNNNNGC 3 cut(s) 697, 837, 846
BstNI CCWGG 3 cut(s) 314, 774, 1062
BstSCI CCNGG 3 cut(s) 312, 772, 1060
BstSFI CTRYAG 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 164
BstYI RGATCY 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 1015
BsuRI GGCC 3 cut(s) 338, 869, 873
BsuTUI ATCGAT 1 cut(s) 1015
BtsCI GGATG 3 cut(s) 153, 170, 319
BtsIMutI CAGTG 1 cut(s) 1164
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 6 cut(s) 86, 405, 514, 847, 871, 1057
CaiI CAGNNNCTG 1 cut(s) 25
CfoI GCGC 2 cut(s) 403, 409
Cfr10I RCCGGY 1 cut(s) 869
Cfr13I GGNCC 4 cut(s) 37, 167, 867, 1064
ClaI ATCGAT 1 cut(s) 1015
Csp6I GTAC 4 cut(s) 531, 618, 982, 1116
CviAII CATG 4 cut(s) 158, 758, 809, 841
CviQI GTAC 4 cut(s) 531, 618, 982, 1116
DdeI CTNAG 5 cut(s) 26, 75, 393, 1167, 1191
DpnI GATC 3 cut(s) 192, 327, 1014
DpnII GATC 3 cut(s) 190, 325, 1012
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 339, 790
Eco24I GRGCYC 1 cut(s) 260
Eco47I GGWCC 3 cut(s) 37, 167, 1064
Eco57I CTGAAG 2 cut(s) 558, 1050
Eco88I CYCGRG 1 cut(s) 1134
EcoO109I RGGNCCY 1 cut(s) 1064
EcoRII CCWGG 3 cut(s) 312, 772, 1060
EcoT14I CCWWGG 2 cut(s) 339, 790
EcoT38I GRGCYC 1 cut(s) 260
ErhI CCWWGG 2 cut(s) 339, 790
Esp3I CGTCTC 1 cut(s) 1134
FaeI CATG 4 cut(s) 161, 761, 812, 844
FaqI GGGAC 2 cut(s) 461, 971
FatI CATG 4 cut(s) 157, 757, 808, 840
FauI CCCGC 1 cut(s) 1165
FblI GTMKAC 1 cut(s) 646
FokI GGATG 3 cut(s) 157, 160, 306
FriOI GRGCYC 1 cut(s) 260
FseI GGCCGGCC 1 cut(s) 873
GlaI GCGC 2 cut(s) 402, 408
GsuI CTGGAG 1 cut(s) 795
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 3 cut(s) 338, 869, 873
HapII CCGG 3 cut(s) 822, 870, 1212
HhaI GCGC 2 cut(s) 403, 409
Hin1II CATG 4 cut(s) 161, 761, 812, 844
Hin6I GCGC 2 cut(s) 401, 407
HinP1I GCGC 2 cut(s) 401, 407
HincII GTYRAC 2 cut(s) 675, 763
HindII GTYRAC 2 cut(s) 675, 763
HinfI GANTC 6 cut(s) 13, 71, 161, 215, 956, 1187
HpaII CCGG 3 cut(s) 822, 870, 1212
HphI GGTGA 2 cut(s) 574, 1123
Hpy166II GTNNAC 4 cut(s) 647, 675, 763, 1219
Hpy188I TCNGA 7 cut(s) 76, 112, 195, 262, 663, 853, 1192
Hpy188III TCNNGA 9 cut(s) 40, 165, 247, 323, 329, 719, 890, 938, 1028
Hpy8I GTNNAC 4 cut(s) 647, 675, 763, 1219
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 5 cut(s) 676, 754, 1025, 1077, 1241
HpyCH4III ACNGT 3 cut(s) 445, 530, 622
HpyCH4IV ACGT 4 cut(s) 571, 858, 984, 1143
HpyCH4V TGCA 9 cut(s) 349, 488, 512, 700, 831, 840, 1055, 1154, 1198
HpyF10VI GCNNNNNNNGC 3 cut(s) 697, 837, 846
HpyF3I CTNAG 5 cut(s) 26, 75, 393, 1167, 1191
HpySE526I ACGT 4 cut(s) 571, 858, 984, 1143
Hsp92II CATG 4 cut(s) 161, 761, 812, 844
HspAI GCGC 2 cut(s) 401, 407
KroI GCCGGC 1 cut(s) 869
KroNI GCCGGC 1 cut(s) 871
Kzo9I GATC 3 cut(s) 190, 325, 1012
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 3 cut(s) 451, 794, 1097
LweI GCATC 4 cut(s) 20, 348, 840, 895
MaeII ACGT 4 cut(s) 571, 858, 984, 1143
MaeIII GTNAC 3 cut(s) 622, 973, 1081
MalI GATC 3 cut(s) 192, 327, 1014
MboI GATC 3 cut(s) 190, 325, 1012
MboII GAAGA 4 cut(s) 551, 698, 1010, 1016
MfeI CAATTG 1 cut(s) 701
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 260
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 6 cut(s) 43, 114, 576, 701, 1001, 1046
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 2 cut(s) 65, 155
Mox20I TGGCCA 1 cut(s) 338
MroNI GCCGGC 1 cut(s) 869
MscI TGGCCA 1 cut(s) 338
MseI TTAA 4 cut(s) 201, 279, 768, 897
MslI CAYNNNNRTG 1 cut(s) 1169
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 1160
MspI CCGG 3 cut(s) 822, 870, 1212
MspR9I CCNGG 3 cut(s) 314, 774, 1062
MunI CAATTG 1 cut(s) 701
MvaI CCWGG 3 cut(s) 314, 774, 1062
MwoI GCNNNNNNNGC 3 cut(s) 697, 837, 846
NaeI GCCGGC 1 cut(s) 871
NdeII GATC 3 cut(s) 190, 325, 1012
NgoMIV GCCGGC 1 cut(s) 869
NlaIII CATG 4 cut(s) 161, 761, 812, 844
NlaIV GGNNCC 1 cut(s) 154
PciSI GCTCTTC 1 cut(s) 681
PdiI GCCGGC 1 cut(s) 871
PfeI GAWTC 4 cut(s) 13, 215, 956, 1187
PflFI GACNNNGTC 1 cut(s) 239
PfoI TCCNGGA 1 cut(s) 772
PleI GAGTC 2 cut(s) 65, 155
PpsI GAGTC 2 cut(s) 65, 155
PpuMI RGGWCCY 1 cut(s) 1064
Psp5II RGGWCCY 1 cut(s) 1064
Psp6I CCWGG 3 cut(s) 312, 772, 1060
PspGI CCWGG 3 cut(s) 312, 772, 1060
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 4 cut(s) 37, 167, 867, 1064
PspPPI RGGWCCY 1 cut(s) 1064
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 239
RigI GGCCGGCC 1 cut(s) 873
RsaI GTAC 4 cut(s) 532, 619, 983, 1117
RsaNI GTAC 4 cut(s) 531, 618, 982, 1116
RseI CAYNNNNRTG 1 cut(s) 1169
SapI GCTCTTC 1 cut(s) 681
SaqAI TTAA 4 cut(s) 201, 279, 768, 897
Sau3AI GATC 3 cut(s) 190, 325, 1012
Sau96I GGNCC 4 cut(s) 37, 167, 867, 1064
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 2 cut(s) 65, 155
ScrFI CCNGG 3 cut(s) 314, 774, 1062
SduI GDGCHC 1 cut(s) 260
SfaNI GCATC 4 cut(s) 20, 348, 840, 895
SfcI CTRYAG 1 cut(s) 17
SinI GGWCC 3 cut(s) 37, 167, 1064
SmiMI CAYNNNNRTG 1 cut(s) 1169
Sse9I AATT 6 cut(s) 43, 114, 576, 701, 1001, 1046
SsiI CCGC 2 cut(s) 66, 1158
SspI AATATT 1 cut(s) 199
StyD4I CCNGG 3 cut(s) 312, 772, 1060
StyI CCWWGG 2 cut(s) 339, 790
TaaI ACNGT 3 cut(s) 445, 530, 622
TaiI ACGT 4 cut(s) 574, 861, 987, 1146
TaqI TCGA 4 cut(s) 246, 834, 1015, 1234
TaqII GACCGA 1 cut(s) 25
TasI AATT 6 cut(s) 43, 114, 576, 701, 1001, 1046
TatI WGTACW 2 cut(s) 530, 617
TfiI GAWTC 4 cut(s) 13, 215, 956, 1187
Tru1I TTAA 4 cut(s) 201, 279, 768, 897
Tru9I TTAA 4 cut(s) 201, 279, 768, 897
TscAI CASTG 1 cut(s) 1171
TspDTI ATGAA 3 cut(s) 228, 458, 816
TspRI CASTG 1 cut(s) 1171
Tth111I GACNNNGTC 1 cut(s) 239
VpaK11BI GGWCC 3 cut(s) 37, 167, 1064
XapI RAATTY 2 cut(s) 1001, 1046
XmiI GTMKAC 1 cut(s) 646
ZrmI AGTACT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.