pycom16g03520

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001016
Physical Location & Seq
Forward (+)
2183084 .. 2185730
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03520.5

Sequence Viewer

Length: 1320 bp
ATGGAGCCGCCGTGGGACAAGAACATGAAGAAGAAGCGCAAGCGGATCCGCCGCCCCGTCAGGCTGAGATGCTCCGTCAAGAATTACGATTGGGGTATTTTCGGCCGCAATTCCAAGGTCGCCAGGCTTTTCGCGCTCAATTCCGGGTCCGACATCGACTCGGGCAGACCGTACGCCGAGTTCTGGATCGGGACTCATGAATCTGGGCCGTCCTATGCGGATTCCGGGTCGGAGCCCTTGAGTCTCAAGGCCTGGATCTCGCAGGATCCCGGTGTGCTTGGCGACAAGGTGGTTGACAAGTGGGGTGCTGATCTTCCGTTCTTGTTTAAGGTGCTTTCGGTTGGGAAGGCGTTGTCGATACAGGCTCATCCGGATAAAGAACTGGCTAGGGTGTTGCACAAGTTACACCCAAATGTTTATAGAGATGACAATCACAAGCCTGAAATGGCATTGGCATTGACGGAGTTCGAGGCACTTTGTGGGTTTATCAGTGTCAAGGAGCTCAAGGACATGCTTAATAGTGTTCCCGAGATTGCAGAATTGGTTGTAGTTGCAGATGCAGAGCGAATTCTACTTTGTAGTGAGCATCATGAGAATGGGAAAGCAAAAACAGATATAGAGTCCATCTTCACTCGACTTATGTTACTAAGTAAAGATACAATTGCTGACATGATTTCTAGACTGAAAAGACGTCTTAATTTAGAGAAAAAGAAAAGGCTTTTGACGGTTAAAGAACAACTGGTACTCAGATTGGAGAGTCAATACCCAGATGATGTTGGTGCAATAGCAGCGTTTTTTCTTAACTATGTGAAGCTGAACCGTGGTGAAGCAATTAGTCTTGGGCCGAATGAACCTCATGCATATATTTCTGGTGAATGCATAGAGTGTATGGCAACTTCTGACAATGTTGTCCGTGCCGGCCTTACTTCAAAACCACTGGATGTTCAAACACTTCTTTCTATGCTCAAATACAGACAGGGTTCTCCAGAAATTATGCAAGGCGTTTCTTTGAATCCATACACAACAAGGTATCTCCCACCTTTTGAAGAATTTGAAGTCGACTGCTGTAACATTCCTCGCTCTGCATCGGTGGTTTTTCCTTCAGTCCTAGGACCATCTCTTTTCATGTTTACAGCCGGGAAGGGACATTTTGATGCAAGTCTGCCTGAGGATGATATAGTGGTGGAGGGTGATGATTTAGTTGAAGAGGGTGAGGTTTTCTTTGTGCCTGCCAACACGAAGATTAGCATTACCGCCAAATCAACAGAACTACAATTGTATAGGGTGGGAGTGAACAGCAGATTTTTTGGGGATTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

440

Amino Acids

49.12

Weight (kDa)

6.2

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 908
AatII GACGTC 1 cut(s) 694
AccI GTMKAC 1 cut(s) 1059
AccII CGCG 1 cut(s) 134
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 7 cut(s) 8, 43, 49, 52, 106, 218, 1254
AclWI GGATC 6 cut(s) 40, 53, 194, 260, 263, 273
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 567, 1049
AcuI CTGAAG 1 cut(s) 1086
AcyI GRCGYC 1 cut(s) 691
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 2 cut(s) 173, 744
AfiI CCNNNNNNNGG 1 cut(s) 183
AgsI TTSAA 6 cut(s) 930, 947, 1012, 1046, 1055, 1205
AjnI CCWGG 2 cut(s) 122, 251
AluBI AGCT 2 cut(s) 502, 814
AluI AGCT 2 cut(s) 502, 814
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 6 cut(s) 40, 53, 194, 260, 263, 273
Ama87I CYCGRG 2 cut(s) 160, 527
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 5 cut(s) 103, 206, 249, 842, 919
ApeKI GCWGC 1 cut(s) 788
ApoI RAATTY 2 cut(s) 567, 1049
AspA2I CCTAGG 1 cut(s) 1108
AspLEI GCGC 2 cut(s) 39, 136
AspS9I GGNCC 4 cut(s) 147, 206, 842, 1112
AsuC2I CCSGG 4 cut(s) 145, 226, 270, 1138
AsuHPI GGTGA 4 cut(s) 836, 884, 1202, 1223
AvaI CYCGRG 2 cut(s) 160, 527
AvaII GGWCC 2 cut(s) 147, 1112
AvrII CCTAGG 1 cut(s) 1108
AxyI CCTNAGG 1 cut(s) 1167
BamHI GGATCC 2 cut(s) 45, 265
BanII GRGCYC 2 cut(s) 237, 504
BarI GAAGNNNNNNTAC 2 cut(s) 880, 912
Bbv12I GWGCWC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 800
BccI CCATC 2 cut(s) 632, 1123
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 2 cut(s) 124, 253
BcnI CCSGG 4 cut(s) 145, 226, 270, 1138
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 3 cut(s) 387, 678, 1109
BisI GCNGC 4 cut(s) 8, 52, 106, 789
BlnI CCTAGG 1 cut(s) 1108
BlsI GCNGC 4 cut(s) 9, 53, 107, 790
Bme1390I CCNGG 6 cut(s) 124, 145, 226, 253, 270, 1138
Bme18I GGWCC 2 cut(s) 147, 1112
BmeT110I CYCGRG 2 cut(s) 160, 527
BmgT120I GGNCC 4 cut(s) 147, 206, 842, 1112
BmiI GGNNCC 5 cut(s) 6, 47, 148, 234, 267
BmrFI CCNGG 6 cut(s) 124, 145, 226, 253, 270, 1138
BmsI GCATC 5 cut(s) 59, 547, 595, 1094, 1144
BpmI CTGGAG 1 cut(s) 969
BpuEI CTTGAG 3 cut(s) 230, 259, 488
BpuMI CCSGG 4 cut(s) 145, 226, 270, 1138
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 691
BsaJI CCNNGG 4 cut(s) 11, 114, 820, 1108
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse118I RCCGGY 1 cut(s) 917
Bse1I ACTGG 3 cut(s) 387, 744, 942
Bse21I CCTNAGG 1 cut(s) 1167
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 2 cut(s) 124, 253
BseDI CCNNGG 4 cut(s) 11, 114, 820, 1108
BseGI GGATG 3 cut(s) 367, 946, 1177
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMII CTCAG 3 cut(s) 56, 760, 1158
BseNI ACTGG 3 cut(s) 387, 744, 942
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 1 cut(s) 800
Bsh1236I CGCG 1 cut(s) 134
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 5 cut(s) 105, 208, 251, 844, 921
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 504
BsiHKCI CYCGRG 2 cut(s) 160, 527
BsiSI CCGG 6 cut(s) 144, 225, 270, 371, 918, 1137
BsiWI CGTACG 1 cut(s) 171
BslFI GGGAC 3 cut(s) 29, 205, 1158
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 248
BsmFI GGGAC 3 cut(s) 29, 205, 1158
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 5 cut(s) 105, 208, 251, 844, 921
BsoBI CYCGRG 2 cut(s) 160, 527
Bsp1286I GDGCHC 2 cut(s) 237, 504
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 5 cut(s) 45, 186, 255, 265, 310
BspACI CCGC 7 cut(s) 8, 43, 49, 52, 106, 218, 1254
BspANI GGCC 5 cut(s) 105, 208, 251, 844, 921
BspCNI CTCAG 3 cut(s) 57, 759, 1159
BspEI TCCGGA 1 cut(s) 370
BspFNI CGCG 1 cut(s) 134
BspHI TCATGA 2 cut(s) 196, 589
BspLI GGNNCC 5 cut(s) 6, 47, 148, 234, 267
BspPI GGATC 6 cut(s) 40, 53, 194, 260, 263, 273
BsrFI RCCGGY 1 cut(s) 917
BsrI ACTGG 3 cut(s) 387, 744, 942
BssAI RCCGGY 1 cut(s) 917
BssECI CCNNGG 4 cut(s) 11, 114, 820, 1108
BssMI GATC 5 cut(s) 45, 186, 255, 265, 310
BssNI GRCGYC 1 cut(s) 691
BssT1I CCWWGG 2 cut(s) 114, 1108
Bst2UI CCWGG 2 cut(s) 124, 253
Bst4CI ACNGT 3 cut(s) 171, 727, 821
Bst6I CTCTTC 1 cut(s) 1200
BstACI GRCGYC 1 cut(s) 691
BstC8I GCNNGC 3 cut(s) 41, 919, 1230
BstDEI CTNAG 4 cut(s) 65, 647, 746, 1167
BstDSI CCRYGG 2 cut(s) 11, 820
BstF5I GGATG 3 cut(s) 367, 946, 1177
BstFNI CGCG 1 cut(s) 134
BstHHI GCGC 2 cut(s) 39, 136
BstKTI GATC 5 cut(s) 48, 189, 258, 268, 313
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 5 cut(s) 45, 186, 255, 265, 310
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 133, 788
BstNI CCWGG 2 cut(s) 124, 253
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 6 cut(s) 122, 143, 224, 251, 268, 1136
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 1 cut(s) 800
BstX2I RGATCY 3 cut(s) 45, 255, 265
BstYI RGATCY 3 cut(s) 45, 255, 265
BstZI CGGCCG 1 cut(s) 103
Bsu36I CCTNAGG 1 cut(s) 1167
BsuRI GGCC 5 cut(s) 105, 208, 251, 844, 921
BtgI CCRYGG 2 cut(s) 11, 820
BtsCI GGATG 3 cut(s) 367, 946, 1177
BtsIMutI CAGTG 2 cut(s) 496, 935
Cac8I GCNNGC 3 cut(s) 41, 919, 1230
CciI TCATGA 2 cut(s) 196, 589
CfoI GCGC 2 cut(s) 39, 136
Cfr10I RCCGGY 1 cut(s) 917
Cfr13I GGNCC 4 cut(s) 147, 206, 842, 1112
Csp6I GTAC 2 cut(s) 172, 743
CviAII CATG 7 cut(s) 25, 197, 511, 590, 670, 857, 1126
CviQI GTAC 2 cut(s) 172, 743
DdeI CTNAG 4 cut(s) 65, 647, 746, 1167
DpnI GATC 5 cut(s) 47, 188, 257, 267, 312
DpnII GATC 5 cut(s) 45, 186, 255, 265, 310
DraIII CACNNNGTG 1 cut(s) 479
DrdI GACNNNNNNGTC 1 cut(s) 908
DseDI GACNNNNNNGTC 1 cut(s) 908
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 1200
EarI CTCTTC 1 cut(s) 1200
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 502
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 114, 1108
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 2 cut(s) 237, 504
Eco47I GGWCC 2 cut(s) 147, 1112
Eco52I CGGCCG 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 1086
Eco81I CCTNAGG 1 cut(s) 1167
Eco88I CYCGRG 2 cut(s) 160, 527
EcoICRI GAGCTC 1 cut(s) 502
EcoRI GAATTC 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 122, 251
EcoT14I CCWWGG 2 cut(s) 114, 1108
EcoT22I ATGCAT 2 cut(s) 862, 881
EcoT38I GRGCYC 2 cut(s) 237, 504
ErhI CCWWGG 2 cut(s) 114, 1108
FaeI CATG 7 cut(s) 28, 200, 514, 593, 673, 860, 1129
FaqI GGGAC 3 cut(s) 29, 205, 1158
FatI CATG 7 cut(s) 24, 196, 510, 589, 669, 856, 1125
FblI GTMKAC 1 cut(s) 1059
Fnu4HI GCNGC 4 cut(s) 8, 52, 106, 789
FokI GGATG 3 cut(s) 354, 953, 1184
FriOI GRGCYC 2 cut(s) 237, 504
Fsp4HI GCNGC 4 cut(s) 8, 52, 106, 789
FspBI CTAG 3 cut(s) 387, 678, 1109
GlaI GCGC 2 cut(s) 38, 135
GluI GCNGC 4 cut(s) 8, 52, 106, 789
GsuI CTGGAG 1 cut(s) 969
HaeIII GGCC 5 cut(s) 105, 208, 251, 844, 921
HapII CCGG 6 cut(s) 144, 225, 270, 371, 918, 1137
HhaI GCGC 2 cut(s) 39, 136
Hin1I GRCGYC 1 cut(s) 691
Hin1II CATG 7 cut(s) 28, 200, 514, 593, 673, 860, 1129
Hin6I GCGC 2 cut(s) 37, 134
HinP1I GCGC 2 cut(s) 37, 134
HincII GTYRAC 2 cut(s) 295, 1060
HindII GTYRAC 2 cut(s) 295, 1060
HinfI GANTC 9 cut(s) 158, 193, 200, 221, 241, 620, 757, 1012, 1313
HpaII CCGG 6 cut(s) 144, 225, 270, 371, 918, 1137
HphI GGTGA 4 cut(s) 836, 884, 1202, 1223
Hpy166II GTNNAC 4 cut(s) 295, 1060, 1131, 1294
Hpy188I TCNGA 4 cut(s) 151, 232, 749, 901
Hpy188III TCNNGA 9 cut(s) 79, 184, 190, 197, 371, 527, 590, 678, 986
Hpy8I GTNNAC 4 cut(s) 295, 1060, 1131, 1294
HpyAV CCTTC 3 cut(s) 340, 1110, 1135
HpyCH4III ACNGT 3 cut(s) 171, 727, 821
HpyCH4IV ACGT 1 cut(s) 691
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 788
HpyF3I CTNAG 4 cut(s) 65, 647, 746, 1167
HpySE526I ACGT 1 cut(s) 691
Hsp92I GRCGYC 1 cut(s) 691
Hsp92II CATG 7 cut(s) 28, 200, 514, 593, 673, 860, 1129
HspAI GCGC 2 cut(s) 37, 134
Kpn2I TCCGGA 1 cut(s) 370
KroI GCCGGC 1 cut(s) 917
KroNI GCCGGC 1 cut(s) 919
Kzo9I GATC 5 cut(s) 45, 186, 255, 265, 310
LmnI GCTCC 4 cut(s) 4, 77, 232, 499
Lsp1109I GCAGC 1 cut(s) 800
LweI GCATC 5 cut(s) 59, 547, 595, 1094, 1144
MaeI CTAG 3 cut(s) 387, 678, 1109
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 3 cut(s) 402, 642, 1067
MalI GATC 5 cut(s) 47, 188, 257, 267, 312
MboI GATC 5 cut(s) 45, 186, 255, 265, 310
MboII GAAGA 7 cut(s) 40, 43, 305, 619, 1058, 1217, 1252
MfeI CAATTG 2 cut(s) 660, 1274
MflI RGATCY 3 cut(s) 45, 255, 265
MhlI GDGCHC 2 cut(s) 237, 504
MlyI GAGTC 5 cut(s) 152, 187, 250, 629, 766
MmeI TCCRAC 2 cut(s) 174, 210
MnlI CCTC 7 cut(s) 463, 864, 1086, 1162, 1180, 1201, 1207
Mph1103I ATGCAT 2 cut(s) 862, 881
MroI TCCGGA 1 cut(s) 370
MroNI GCCGGC 1 cut(s) 917
MseI TTAA 5 cut(s) 327, 516, 696, 729, 801
MslI CAYNNNNRTG 3 cut(s) 411, 594, 1152
MspI CCGG 6 cut(s) 144, 225, 270, 371, 918, 1137
MspR9I CCNGG 6 cut(s) 124, 145, 226, 253, 270, 1138
MunI CAATTG 2 cut(s) 660, 1274
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 2 cut(s) 124, 253
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 2 cut(s) 133, 788
NaeI GCCGGC 1 cut(s) 919
NciI CCSGG 4 cut(s) 145, 226, 270, 1138
NdeII GATC 5 cut(s) 45, 186, 255, 265, 310
NgoMIV GCCGGC 1 cut(s) 917
NlaIII CATG 7 cut(s) 28, 200, 514, 593, 673, 860, 1129
NlaIV GGNNCC 5 cut(s) 6, 47, 148, 234, 267
NmeAIII GCCGAG 1 cut(s) 202
NsiI ATGCAT 2 cut(s) 862, 881
NspI RCATGY 1 cut(s) 514
PagI TCATGA 2 cut(s) 196, 589
PceI AGGCCT 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 1 cut(s) 881
PdiI GCCGGC 1 cut(s) 919
PfeI GAWTC 4 cut(s) 200, 221, 1012, 1313
Pfl23II CGTACG 1 cut(s) 171
PkrI GCNGC 4 cut(s) 9, 53, 107, 790
PleI GAGTC 5 cut(s) 152, 187, 249, 628, 765
PpsI GAGTC 5 cut(s) 152, 187, 249, 628, 765
Psp124BI GAGCTC 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 122, 251
PspGI CCWGG 2 cut(s) 122, 251
PspLI CGTACG 1 cut(s) 171
PspN4I GGNNCC 5 cut(s) 6, 47, 148, 234, 267
PspPI GGNCC 4 cut(s) 147, 206, 842, 1112
PsrI GAACNNNNNNTAC 4 cut(s) 164, 196, 726, 758
PsuI RGATCY 3 cut(s) 45, 255, 265
RsaI GTAC 2 cut(s) 173, 744
RsaNI GTAC 2 cut(s) 172, 743
RseI CAYNNNNRTG 3 cut(s) 411, 594, 1152
SacI GAGCTC 1 cut(s) 504
SalI GTCGAC 1 cut(s) 1058
SaqAI TTAA 5 cut(s) 327, 516, 696, 729, 801
SatI GCNGC 4 cut(s) 8, 52, 106, 789
Sau3AI GATC 5 cut(s) 45, 186, 255, 265, 310
Sau96I GGNCC 4 cut(s) 147, 206, 842, 1112
SchI GAGTC 5 cut(s) 152, 187, 250, 629, 766
ScrFI CCNGG 6 cut(s) 124, 145, 226, 253, 270, 1138
SduI GDGCHC 2 cut(s) 237, 504
SfaNI GCATC 5 cut(s) 59, 547, 595, 1094, 1144
SinI GGWCC 2 cut(s) 147, 1112
SmiMI CAYNNNNRTG 3 cut(s) 411, 594, 1152
SmlI CTYRAG 3 cut(s) 238, 245, 503
SmoI CTYRAG 3 cut(s) 238, 245, 503
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 7 cut(s) 8, 43, 49, 52, 106, 218, 1254
SspMI CTAG 3 cut(s) 387, 678, 1109
SstI GAGCTC 1 cut(s) 504
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 6 cut(s) 122, 143, 224, 251, 268, 1136
StyI CCWWGG 2 cut(s) 114, 1108
TaaI ACNGT 3 cut(s) 171, 727, 821
TaiI ACGT 1 cut(s) 694
TaqI TCGA 5 cut(s) 156, 356, 468, 634, 1059
TauI GCSGC 3 cut(s) 10, 54, 108
TfiI GAWTC 4 cut(s) 200, 221, 1012, 1313
Tru1I TTAA 5 cut(s) 327, 516, 696, 729, 801
Tru9I TTAA 5 cut(s) 327, 516, 696, 729, 801
TscAI CASTG 2 cut(s) 496, 942
TseI GCWGC 1 cut(s) 788
TspDTI ATGAA 4 cut(s) 41, 213, 864, 1114
TspGWI ACGGA 4 cut(s) 64, 306, 476, 902
TspRI CASTG 2 cut(s) 496, 942
VpaK11BI GGWCC 2 cut(s) 147, 1112
XapI RAATTY 2 cut(s) 567, 1049
XbaI TCTAGA 1 cut(s) 677
XceI RCATGY 1 cut(s) 514
XmaJI CCTAGG 1 cut(s) 1108
XmiI GTMKAC 1 cut(s) 1059
XspI CTAG 3 cut(s) 387, 678, 1109
ZraI GACGTC 1 cut(s) 692
Zsp2I ATGCAT 2 cut(s) 862, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.