Rh4DG086100

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001016
Physical Location & Seq
Reverse (-)
13098910 .. 13100392
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG086100.1

Sequence Viewer

Length: 1305 bp
ATGGAGATGCAACAACCAAAGCAGAGGCAGAGGAGTGTTCAGAGGCTAAGATGCTCTGTCATGAACTACGCCTGGGGTAAGAAAGGCCGAGACTCTGTGGTGGGCAGGTTGTGTGCCTCCAATTCTCATTCTGAAATCGACCTAGAAAAGCCTTATGCTGAAATGTGGATGGGAACACATGACTCTGGACCCTCCTTTTTGATTGATCAGGATCTGAATAATAATAGCACCACTACTGGGTCATCTCCGTCTCTCAGAGACTGGGTTTCTAACAACCCTAAAGACATGCTTGGTCACAAGGTTGTCCAAAAATGGGGCTCCTCTGATCTGCCTTTCTTGTTTAAGGTGCAATCCGTGGCAAAGGCATTGTCAATACAGGCACACCCAGATAAGGAATTGGCCAAGGCTTTGCACAAGTCTATGCCAGATATTTACAAGGATGATAATTATAAGCCTGAGATGGCGCTGGCCTTGACACATTTTCAGGCTCTTTGTGGTTTTATCACTCTTGAGGAGCTTAAGAAGGTGATTGGTAATGTCCCTGAGATTGTAGAACTCGTTGGTAGTGAGGTCGAAAACCAAGTGCTTCGCACGACCAAAGAAGATGGGGAGGATAAAGTGAAGTCTGTTATACGTTTGATTTTTACCCAACTCATGTCAACTAACAAGGAAATTATAACCACAGAAACAACTAAATTGAAGAGTCGTTTACAGACGGAAAGCCAGGTGAGGCAGCTGACAGAAAAGGAGCAGCTAGTGTTGCAATTGGAAAAGCAATATCCGAACGATGTGGGTGTGATATCTGCATTCTTTCTTAACCATGTGAAGCTTAATCCTGGAGAAGCATTATGCCTTGGAGCAAATGAACCCCATGCCTATATATCTGGGGATTGCATCGAGTGCATGGCAACCTCAGACAACGTAGTTAGGGCTGGCCTTACTCCCAAGCATCGAGATATCAAAACCCTTTGTTCTATGCTCACATATAAGCAGGGTTTGCCTGAAATCTTGCGAGGAGTTGCTGTGAATCCTCATGTAACAAGGTACATACCGCCTTTTGAAGAATTTGAGGTTGATCGGATCCATCTTCCCCACGGAGAATCAACAGAGTTTCCTGCAGTACCTGGTCCTTCCATTTTTGTGGTCACTTTTGGGAAGGGAACCATATGTACGAGTAATGGAGATCATGCAATCATCCAAGGAGATGTTCTGTTTGCACCTGCACACACTGAGATTAGCATAACAAGTGCATCTGAGTTGCACATATATAGAGCCGGAGTGAATAGCACATTCTTAGCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

434

Amino Acids

48.29

Weight (kDa)

6.22

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 13 - 167 1.7e-43 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 186 - 272 1.4e-14 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 450, 677
AarI CACCTGC 1 cut(s) 1228
Acc36I ACCTGC 2 cut(s) 96, 1228
AciI CCGC 1 cut(s) 1052
AclWI GGATC 3 cut(s) 219, 1075, 1088
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 1 cut(s) 1064
AfaI GTAC 3 cut(s) 1046, 1122, 1171
AfiI CCNNNNNNNGG 3 cut(s) 237, 313, 391
AflII CTTAAG 1 cut(s) 518
AgsI TTSAA 2 cut(s) 700, 1061
AjnI CCWGG 4 cut(s) 71, 723, 835, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 1191, 1223
AluBI AGCT 4 cut(s) 517, 736, 754, 829
AluI AGCT 4 cut(s) 517, 736, 754, 829
Alw26I GTCTC 3 cut(s) 84, 252, 255
AlwI GGATC 3 cut(s) 219, 1075, 1088
AlwNI CAGNNNCTG 3 cut(s) 214, 261, 1124
AoxI GGCC 4 cut(s) 85, 399, 468, 934
ApeKI GCWGC 2 cut(s) 733, 751
ApoI RAATTY 1 cut(s) 1064
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 2 cut(s) 188, 1127
AsuHPI GGTGA 2 cut(s) 538, 739
AvaII GGWCC 2 cut(s) 188, 1127
BalI TGGCCA 1 cut(s) 401
BamHI GGATCC 1 cut(s) 1080
BanII GRGCYC 1 cut(s) 320
BbvI GCAGC 2 cut(s) 745, 763
BccI CCATC 4 cut(s) 163, 454, 599, 1092
BciT130I CCWGG 4 cut(s) 73, 725, 837, 1125
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 3 cut(s) 84, 252, 255
BfaI CTAG 2 cut(s) 143, 755
BfmI CTRYAG 1 cut(s) 1116
BfoI RGCGCY 1 cut(s) 467
BfrI CTTAAG 1 cut(s) 518
BfuAI ACCTGC 2 cut(s) 96, 1228
BisI GCNGC 2 cut(s) 734, 752
BlsI GCNGC 2 cut(s) 735, 753
Bme1390I CCNGG 4 cut(s) 73, 725, 837, 1125
Bme18I GGWCC 2 cut(s) 188, 1127
BmgT120I GGNCC 2 cut(s) 188, 1127
BmiI GGNNCC 4 cut(s) 190, 319, 1082, 1162
BmrFI CCNGG 4 cut(s) 73, 725, 837, 1125
BmrI ACTGGG 2 cut(s) 246, 271
BmsI GCATC 4 cut(s) 41, 903, 958, 1259
BmuI ACTGGG 2 cut(s) 246, 271
BpmI CTGGAG 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 530
BsaBI GATNNNNATC 1 cut(s) 210
BsaJI CCNNGG 6 cut(s) 72, 354, 402, 853, 1093, 1198
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 3 cut(s) 237, 313, 391
Bse1I ACTGG 2 cut(s) 241, 266
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 4 cut(s) 73, 725, 837, 1125
BseDI CCNNGG 6 cut(s) 72, 354, 402, 853, 1093, 1198
BseGI GGATG 3 cut(s) 174, 445, 1194
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 3 cut(s) 237, 313, 391
BseMII CTCAG 6 cut(s) 268, 447, 534, 927, 1221, 1245
BseNI ACTGG 2 cut(s) 241, 266
BseRI GAGGAG 4 cut(s) 46, 310, 527, 1029
BseXI GCAGC 2 cut(s) 745, 763
BsgI GTGCAG 1 cut(s) 1206
BshFI GGCC 4 cut(s) 87, 401, 470, 936
BsiSI CCGG 1 cut(s) 1275
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 3 cut(s) 237, 313, 391
BsmAI GTCTC 3 cut(s) 84, 252, 255
BsmBI CGTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 4 cut(s) 87, 401, 470, 936
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
BspACI CCGC 1 cut(s) 1052
BspANI GGCC 4 cut(s) 87, 401, 470, 936
BspCNI CTCAG 6 cut(s) 267, 448, 535, 926, 1222, 1246
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 4 cut(s) 190, 319, 1082, 1162
BspMAI CTGCAG 1 cut(s) 1120
BspMI ACCTGC 2 cut(s) 96, 1228
BspPI GGATC 3 cut(s) 219, 1075, 1088
BspTI CTTAAG 1 cut(s) 518
BsrI ACTGG 2 cut(s) 241, 266
BssECI CCNNGG 6 cut(s) 72, 354, 402, 853, 1093, 1198
BssMI GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
BssT1I CCWWGG 3 cut(s) 402, 853, 1198
Bst2UI CCWGG 4 cut(s) 73, 725, 837, 1125
Bst6I CTCTTC 1 cut(s) 695
BstAFI CTTAAG 1 cut(s) 518
BstAPI GCANNNNNTGC 2 cut(s) 900, 997
BstC8I GCNNGC 2 cut(s) 468, 934
BstDEI CTNAG 8 cut(s) 47, 254, 456, 543, 913, 1230, 1254, 1294
BstDSI CCRYGG 2 cut(s) 354, 1093
BstF5I GGATG 3 cut(s) 174, 445, 1194
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 6 cut(s) 208, 214, 328, 1078, 1083, 1186
BstMAI GTCTC 3 cut(s) 84, 252, 255
BstMBI GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
BstMWI GCNNNNNNNGC 3 cut(s) 760, 900, 997
BstNI CCWGG 4 cut(s) 73, 725, 837, 1125
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 4 cut(s) 71, 723, 835, 1123
BstSFI CTRYAG 1 cut(s) 1116
BstV1I GCAGC 2 cut(s) 745, 763
BstX2I RGATCY 2 cut(s) 211, 1080
BstXI CCANNNNNNTGG 1 cut(s) 1141
BstYI RGATCY 2 cut(s) 211, 1080
BsuRI GGCC 4 cut(s) 87, 401, 470, 936
BtgI CCRYGG 2 cut(s) 354, 1093
BtsCI GGATG 3 cut(s) 174, 445, 1194
BtsIMutI CAGTG 1 cut(s) 1227
BveI ACCTGC 2 cut(s) 96, 1228
Cac8I GCNNGC 2 cut(s) 468, 934
CaiI CAGNNNCTG 3 cut(s) 214, 261, 1124
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 466
Cfr13I GGNCC 2 cut(s) 188, 1127
CsiI ACCWGGT 1 cut(s) 1123
Csp6I GTAC 3 cut(s) 1045, 1121, 1170
CviAII CATG 9 cut(s) 61, 179, 286, 655, 821, 872, 904, 1034, 1187
CviQI GTAC 3 cut(s) 1045, 1121, 1170
DdeI CTNAG 8 cut(s) 47, 254, 456, 543, 913, 1230, 1254, 1294
DpnI GATC 6 cut(s) 207, 213, 327, 1077, 1082, 1185
DpnII GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
Eco130I CCWWGG 3 cut(s) 402, 853, 1198
Eco24I GRGCYC 1 cut(s) 320
Eco32I GATATC 2 cut(s) 801, 958
Eco47I GGWCC 2 cut(s) 188, 1127
EcoRII CCWGG 4 cut(s) 71, 723, 835, 1123
EcoRV GATATC 2 cut(s) 801, 958
EcoT14I CCWWGG 3 cut(s) 402, 853, 1198
EcoT38I GRGCYC 1 cut(s) 320
ErhI CCWWGG 3 cut(s) 402, 853, 1198
Esp3I CGTCTC 1 cut(s) 255
FaeI CATG 9 cut(s) 64, 182, 289, 658, 824, 875, 907, 1037, 1190
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FaqI GGGAC 1 cut(s) 524
FatI CATG 9 cut(s) 60, 178, 285, 654, 820, 871, 903, 1033, 1186
FauNDI CATATG 1 cut(s) 1166
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 734, 752
FokI GGATG 3 cut(s) 181, 452, 1181
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 2 cut(s) 734, 752
FspBI CTAG 2 cut(s) 143, 755
GlaI GCGC 1 cut(s) 465
GluI GCNGC 2 cut(s) 734, 752
GsuI CTGGAG 1 cut(s) 858
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 4 cut(s) 87, 401, 470, 936
HapII CCGG 1 cut(s) 1275
HhaI GCGC 1 cut(s) 466
Hin1II CATG 9 cut(s) 64, 182, 289, 658, 824, 875, 907, 1037, 1190
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 5 cut(s) 92, 182, 703, 1027, 1100
HpaII CCGG 1 cut(s) 1275
HphI GGTGA 2 cut(s) 538, 739
Hpy166II GTNNAC 2 cut(s) 660, 710
Hpy188I TCNGA 9 cut(s) 42, 133, 216, 257, 325, 783, 916, 1080, 1255
Hpy188III TCNNGA 5 cut(s) 61, 186, 209, 509, 953
Hpy8I GTNNAC 2 cut(s) 660, 710
HpyAV CCTTC 3 cut(s) 517, 1140, 1150
HpyCH4IV ACGT 2 cut(s) 634, 921
HpyF10VI GCNNNNNNNGC 3 cut(s) 760, 900, 997
HpyF3I CTNAG 8 cut(s) 47, 254, 456, 543, 913, 1230, 1254, 1294
HpySE526I ACGT 2 cut(s) 634, 921
Hsp92II CATG 9 cut(s) 64, 182, 289, 658, 824, 875, 907, 1037, 1190
HspAI GCGC 1 cut(s) 464
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
LmnI GCTCC 4 cut(s) 323, 514, 748, 857
Lsp1109I GCAGC 2 cut(s) 745, 763
LweI GCATC 4 cut(s) 41, 903, 958, 1259
MabI ACCWGGT 1 cut(s) 1123
MaeI CTAG 2 cut(s) 143, 755
MaeII ACGT 2 cut(s) 634, 921
MaeIII GTNAC 3 cut(s) 293, 1036, 1144
MalI GATC 6 cut(s) 207, 213, 327, 1077, 1082, 1185
MboI GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
MboII GAAGA 4 cut(s) 614, 712, 1073, 1079
MfeI CAATTG 1 cut(s) 764
MflI RGATCY 2 cut(s) 211, 1080
MhlI GDGCHC 1 cut(s) 320
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 7 cut(s) 121, 395, 445, 672, 695, 764, 1064
MluNI TGGCCA 1 cut(s) 401
MlyI GAGTC 3 cut(s) 86, 176, 712
Mox20I TGGCCA 1 cut(s) 401
MscI TGGCCA 1 cut(s) 401
MseI TTAA 4 cut(s) 342, 519, 816, 831
MslI CAYNNNNRTG 1 cut(s) 1139
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 736
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 1 cut(s) 1275
MspR9I CCNGG 4 cut(s) 73, 725, 837, 1125
MunI CAATTG 1 cut(s) 764
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 4 cut(s) 73, 725, 837, 1125
MwoI GCNNNNNNNGC 3 cut(s) 760, 900, 997
NdeI CATATG 1 cut(s) 1166
NdeII GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
NlaIII CATG 9 cut(s) 64, 182, 289, 658, 824, 875, 907, 1037, 1190
NlaIV GGNNCC 4 cut(s) 190, 319, 1082, 1162
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 2 cut(s) 293, 1144
NspI RCATGY 1 cut(s) 289
PagI TCATGA 1 cut(s) 60
PaqCI CACCTGC 1 cut(s) 1228
PctI GAATGC 1 cut(s) 806
PfeI GAWTC 2 cut(s) 1027, 1100
PfoI TCCNGGA 1 cut(s) 835
PkrI GCNGC 2 cut(s) 735, 753
PleI GAGTC 3 cut(s) 86, 176, 711
PpsI GAGTC 3 cut(s) 86, 176, 711
PsiI TTATAA 2 cut(s) 450, 677
Psp6I CCWGG 4 cut(s) 71, 723, 835, 1123
PspGI CCWGG 4 cut(s) 71, 723, 835, 1123
PspN4I GGNNCC 4 cut(s) 190, 319, 1082, 1162
PspPI GGNCC 2 cut(s) 188, 1127
PsrI GAACNNNNNNTAC 2 cut(s) 1153, 1185
PstI CTGCAG 1 cut(s) 1120
PstNI CAGNNNCTG 3 cut(s) 214, 261, 1124
PsuI RGATCY 2 cut(s) 211, 1080
PvuII CAGCTG 1 cut(s) 736
RsaI GTAC 3 cut(s) 1046, 1122, 1171
RsaNI GTAC 3 cut(s) 1045, 1121, 1170
RseI CAYNNNNRTG 1 cut(s) 1139
SaqAI TTAA 4 cut(s) 342, 519, 816, 831
SatI GCNGC 2 cut(s) 734, 752
Sau3AI GATC 6 cut(s) 205, 211, 325, 1075, 1080, 1183
Sau96I GGNCC 2 cut(s) 188, 1127
SchI GAGTC 3 cut(s) 86, 176, 712
ScrFI CCNGG 4 cut(s) 73, 725, 837, 1125
SduI GDGCHC 1 cut(s) 320
SexAI ACCWGGT 1 cut(s) 1123
SfaNI GCATC 4 cut(s) 41, 903, 958, 1259
SfcI CTRYAG 1 cut(s) 1116
SinI GGWCC 2 cut(s) 188, 1127
SmiMI CAYNNNNRTG 1 cut(s) 1139
SmlI CTYRAG 2 cut(s) 509, 518
SmoI CTYRAG 2 cut(s) 509, 518
Sse9I AATT 7 cut(s) 121, 395, 445, 672, 695, 764, 1064
SsiI CCGC 1 cut(s) 1052
SspMI CTAG 2 cut(s) 143, 755
StyD4I CCNGG 4 cut(s) 71, 723, 835, 1123
StyI CCWWGG 3 cut(s) 402, 853, 1198
TaiI ACGT 2 cut(s) 637, 924
TaqI TCGA 4 cut(s) 138, 573, 897, 952
TasI AATT 7 cut(s) 121, 395, 445, 672, 695, 764, 1064
TfiI GAWTC 2 cut(s) 1027, 1100
Tru1I TTAA 4 cut(s) 342, 519, 816, 831
Tru9I TTAA 4 cut(s) 342, 519, 816, 831
TscAI CASTG 1 cut(s) 1234
TseFI GTSAC 2 cut(s) 293, 1144
TseI GCWGC 2 cut(s) 733, 751
Tsp45I GTSAC 2 cut(s) 293, 1144
TspDTI ATGAA 2 cut(s) 77, 879
TspGWI ACGGA 4 cut(s) 237, 343, 731, 1110
TspRI CASTG 1 cut(s) 1234
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 188, 1127
XapI RAATTY 1 cut(s) 1064
XceI RCATGY 1 cut(s) 289
XspI CTAG 2 cut(s) 143, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.