FvH4_4g27070

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001016
Physical Location & Seq
Forward (+)
28215650 .. 28218569
2920 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27070.t2

Sequence Viewer

Length: 1314 bp
ATGGAGCCCTGGAAGAACGCCGCCATGAAGAAGAAGCGGAGGCCCGTCCGGCTCCGCTGCTCCGTCAAGAACTACGACTGGGGCATTCTCGGCCACCATTCCAAGGTCGCCCGCCTCTTCGCTCTCAATTCCGGCTCCGACATCGACCCCGCCAAGCCCTACGCCGAGTTCTGGATCGGCACTCATGAATCCGGCCCCTCCTTTGCCGAGTCCGGGTCCGAAGAGGACCCGGTCAGCCTCAAGTCGTGGATCGCCCGGGATCCGAGTGTTCTCGGCCAGAAGGTTCTCGACAAGTGGGGCCCCGATCTTCCGTTCTTGTGCAAGGTACTTTCGGTTCAGAAGGCTTTGTCGATACAGGCTCATCCGGATAAAGAGATGGCTAGAGTGCTGCACAAGTTGCACCCTGGTGTTTATAGAGATGATAATCACAAGCCTGAAGTGGCATTGGCGCTGACGGAGTTTGAGGCTCTTTGTGGCTTTATTAGTCTCAAGGAGCTAAAAGAGATGCTTCGTACTATTCCTGAGATAGCCGAATTGGTTGGGGTGGCAGATGCAGAGAAAATTCTACTTGTTGGTGAGCGACATGAGAGTGGGAAAGTAAACACAGATTTAGAGGCCATGTTCAGTCGACTTATGTTGTCAAGCAAAGAGACAATTTCCAAGATGATATCCAAACTGAAAAGGCGTCTTAATCAAGAGAAAAAGAAAAGGCAATTGACGGTTAAAGAACAGCTGGTACTCAGATTAGAGAATCAATATCCAGCTGATGTGGGCGCTATAGCTGCATTTTTCCTAAACTATGTGAAGCTGAAACGTGGTGAAGCATTATATTGTGGACCAAATGAACCCCATGCATATATTTCTGGTGAATGTGTCGAATGTATGGCTACCTCTGACAATGTTGTTCGAGCCGGCCTTACCTCAAAACAAATTGATGTTCAGACACTTCTTTCTATGCTCAAATACAGACAGGGAGCTCCTGAAATTATGCAAGGAGTTTCTCTGAACGCATACACAACAAGATTTATCCCACCATTTGAAGAATTTGAAGTCGACTCCTGTAATCTTCCTCATTCTGCTTCGGTAGTTTTTCCTTCCTTCATAGGACCATCCCTTTTTCTAGTCACTGCTGGGAAAGGCAGGTTTGATGCGGGCTTGGCTGGGGATGATGTAGTTACAGAAGATGATGATATAGTTGAGGAGGGCGAGGTTCTATTTGTGCCTGCCCACACCAAGATTAGCATTACTGCTAGATCAACAGAACTACATTTGTATAGGGTGGGGGCAAACAGCACATTTTTTAGGGACCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

438

Amino Acids

48.74

Weight (kDa)

6.66

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 17 - 160 3.4e-43 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 193 - 265 6.8e-12 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1131
AccI GTMKAC 2 cut(s) 628, 1053
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 6 cut(s) 21, 37, 55, 112, 150, 1151
AclWI GGATC 4 cut(s) 182, 254, 257, 267
AcoI YGGCCR 2 cut(s) 91, 274
AcsI RAATTY 2 cut(s) 561, 1043
AcuI CTGAAG 1 cut(s) 456
AcyI GRCGYC 1 cut(s) 685
AfaI GTAC 3 cut(s) 327, 514, 738
AfiI CCNNNNNNNGG 3 cut(s) 103, 171, 213
AgsI TTSAA 2 cut(s) 1040, 1049
AjnI CCWGG 2 cut(s) 8, 403
AleI CACNNNNGTG 1 cut(s) 405
AluBI AGCT 6 cut(s) 496, 733, 764, 782, 808, 977
AluI AGCT 6 cut(s) 496, 733, 764, 782, 808, 977
Alw21I GWGCWC 1 cut(s) 979
Alw26I GTCTC 2 cut(s) 491, 644
AlwI GGATC 4 cut(s) 182, 254, 257, 267
Ama87I CYCGRG 1 cut(s) 255
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 7 cut(s) 41, 91, 193, 274, 298, 615, 913
ApaI GGGCCC 1 cut(s) 302
ApeKI GCWGC 3 cut(s) 57, 388, 782
ApoI RAATTY 2 cut(s) 561, 1043
AspLEI GCGC 2 cut(s) 451, 776
AspS9I GGNCC 9 cut(s) 42, 194, 216, 226, 298, 299, 836, 1106, 1306
AsuC2I CCSGG 4 cut(s) 214, 230, 256, 257
AsuHPI GGTGA 3 cut(s) 587, 830, 878
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 5 cut(s) 216, 226, 836, 1106, 1306
BaeGI GKGCMC 1 cut(s) 302
BamHI GGATCC 1 cut(s) 259
BanII GRGCYC 3 cut(s) 9, 302, 979
Bbv12I GWGCWC 1 cut(s) 979
BbvI GCAGC 3 cut(s) 44, 375, 769
BccI CCATC 2 cut(s) 370, 1117
BciT130I CCWGG 2 cut(s) 10, 405
BcnI CCSGG 4 cut(s) 214, 230, 256, 257
BcoDI GTCTC 2 cut(s) 491, 644
BfaI CTAG 3 cut(s) 381, 1121, 1251
BfmI CTRYAG 2 cut(s) 777, 1310
BfoI RGCGCY 2 cut(s) 452, 777
BfuAI ACCTGC 1 cut(s) 1131
BglI GCCNNNNNGGC 1 cut(s) 49
BisI GCNGC 4 cut(s) 21, 58, 389, 783
BlsI GCNGC 4 cut(s) 22, 59, 390, 784
Bme1390I CCNGG 6 cut(s) 10, 214, 230, 256, 257, 405
Bme18I GGWCC 5 cut(s) 216, 226, 836, 1106, 1306
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 9 cut(s) 42, 194, 216, 226, 298, 299, 836, 1106, 1306
BmrFI CCNGG 6 cut(s) 10, 214, 230, 256, 257, 405
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 3 cut(s) 495, 541, 1138
BmuI ACTGGG 1 cut(s) 88
BpuEI CTTGAG 2 cut(s) 224, 473
BpuMI CCSGG 4 cut(s) 214, 230, 256, 257
BsaBI GATNNNNATC 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 685
BsaJI CCNNGG 4 cut(s) 8, 102, 255, 403
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 171, 213
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 1 cut(s) 83
Bse8I GATNNNNATC 1 cut(s) 423
BseAI TCCGGA 1 cut(s) 364
BseBI CCWGG 2 cut(s) 10, 405
BseDI CCNNGG 4 cut(s) 8, 102, 255, 403
BseGI GGATG 3 cut(s) 361, 1109, 1171
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 3 cut(s) 103, 171, 213
BseMII CTCAG 2 cut(s) 513, 754
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 1214
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 3 cut(s) 44, 375, 769
BseYI CCCAGC 2 cut(s) 1130, 1160
BsgI GTGCAG 1 cut(s) 374
BshFI GGCC 7 cut(s) 43, 93, 195, 276, 300, 617, 915
BsiHKAI GWGCWC 1 cut(s) 979
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 8 cut(s) 49, 132, 192, 213, 230, 256, 365, 912
BslI CCNNNNNNNGG 3 cut(s) 103, 171, 213
BsmAI GTCTC 2 cut(s) 491, 644
BsmI GAATGC 1 cut(s) 84
BsnI GGCC 7 cut(s) 43, 93, 195, 276, 300, 617, 915
BsoBI CYCGRG 1 cut(s) 255
Bsp120I GGGCCC 1 cut(s) 298
Bsp1286I GDGCHC 3 cut(s) 9, 302, 979
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 5 cut(s) 174, 249, 259, 304, 1253
BspACI CCGC 6 cut(s) 21, 37, 55, 112, 150, 1151
BspANI GGCC 7 cut(s) 43, 93, 195, 276, 300, 617, 915
BspCNI CTCAG 2 cut(s) 514, 753
BspEI TCCGGA 1 cut(s) 364
BspHI TCATGA 1 cut(s) 184
BspMI ACCTGC 1 cut(s) 1131
BspPI GGATC 4 cut(s) 182, 254, 257, 267
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 4 cut(s) 8, 102, 255, 403
BssMI GATC 5 cut(s) 174, 249, 259, 304, 1253
BssNI GRCGYC 1 cut(s) 685
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 2 cut(s) 10, 405
Bst4CI ACNGT 1 cut(s) 721
Bst6I CTCTTC 2 cut(s) 122, 216
BstACI GRCGYC 1 cut(s) 685
BstAPI GCANNNNNTGC 1 cut(s) 397
BstC8I GCNNGC 4 cut(s) 112, 913, 1153, 1224
BstDEI CTNAG 2 cut(s) 522, 740
BstF5I GGATG 3 cut(s) 361, 1109, 1171
BstH2I RGCGCY 2 cut(s) 452, 777
BstHHI GCGC 2 cut(s) 451, 776
BstKTI GATC 5 cut(s) 177, 252, 262, 307, 1256
BstMAI GTCTC 2 cut(s) 491, 644
BstMBI GATC 5 cut(s) 174, 249, 259, 304, 1253
BstMWI GCNNNNNNNGC 5 cut(s) 49, 90, 397, 782, 1157
BstNI CCWGG 2 cut(s) 10, 405
BstSCI CCNGG 6 cut(s) 8, 212, 228, 254, 255, 403
BstSFI CTRYAG 2 cut(s) 777, 1310
BstSLI GKGCMC 1 cut(s) 302
BstV1I GCAGC 3 cut(s) 44, 375, 769
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BsuRI GGCC 7 cut(s) 43, 93, 195, 276, 300, 617, 915
BtsCI GGATG 3 cut(s) 361, 1109, 1171
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 1 cut(s) 1125
BveI ACCTGC 1 cut(s) 1131
Cac8I GCNNGC 4 cut(s) 112, 913, 1153, 1224
CciI TCATGA 1 cut(s) 184
CfoI GCGC 2 cut(s) 451, 776
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 9 cut(s) 42, 194, 216, 226, 298, 299, 836, 1106, 1306
Cfr9I CCCGGG 1 cut(s) 255
CseI GACGC 1 cut(s) 674
Csp6I GTAC 3 cut(s) 326, 513, 737
CviAII CATG 5 cut(s) 25, 185, 584, 619, 851
CviQI GTAC 3 cut(s) 326, 513, 737
DdeI CTNAG 2 cut(s) 522, 740
DpnI GATC 5 cut(s) 176, 251, 261, 306, 1255
DpnII GATC 5 cut(s) 174, 249, 259, 304, 1253
EaeI YGGCCR 2 cut(s) 91, 274
Eam1104I CTCTTC 2 cut(s) 122, 216
EarI CTCTTC 2 cut(s) 122, 216
Ecl136II GAGCTC 1 cut(s) 977
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 3 cut(s) 9, 302, 979
Eco32I GATATC 1 cut(s) 669
Eco47I GGWCC 5 cut(s) 216, 226, 836, 1106, 1306
Eco53kI GAGCTC 1 cut(s) 977
Eco57I CTGAAG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 977
EcoO109I RGGNCCY 4 cut(s) 226, 298, 299, 1306
EcoRII CCWGG 2 cut(s) 8, 403
EcoRV GATATC 1 cut(s) 669
EcoT14I CCWWGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 856
EcoT38I GRGCYC 3 cut(s) 9, 302, 979
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 5 cut(s) 28, 188, 587, 622, 854
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FatI CATG 5 cut(s) 24, 184, 583, 618, 850
FauI CCCGC 3 cut(s) 119, 157, 1144
FblI GTMKAC 2 cut(s) 628, 1053
Fnu4HI GCNGC 4 cut(s) 21, 58, 389, 783
FokI GGATG 3 cut(s) 348, 1096, 1178
FriOI GRGCYC 3 cut(s) 9, 302, 979
Fsp4HI GCNGC 4 cut(s) 21, 58, 389, 783
FspBI CTAG 3 cut(s) 381, 1121, 1251
GlaI GCGC 2 cut(s) 450, 775
GluI GCNGC 4 cut(s) 21, 58, 389, 783
GsaI CCCAGC 2 cut(s) 1134, 1164
HaeII RGCGCY 2 cut(s) 452, 777
HaeIII GGCC 7 cut(s) 43, 93, 195, 276, 300, 617, 915
HapII CCGG 8 cut(s) 49, 132, 192, 213, 230, 256, 365, 912
HgaI GACGC 1 cut(s) 674
HhaI GCGC 2 cut(s) 451, 776
Hin1I GRCGYC 1 cut(s) 685
Hin1II CATG 5 cut(s) 28, 188, 587, 622, 854
Hin6I GCGC 2 cut(s) 449, 774
HinP1I GCGC 2 cut(s) 449, 774
HincII GTYRAC 2 cut(s) 629, 1054
HindII GTYRAC 2 cut(s) 629, 1054
HinfI GANTC 4 cut(s) 188, 209, 751, 1055
HpaII CCGG 8 cut(s) 49, 132, 192, 213, 230, 256, 365, 912
HphI GGTGA 3 cut(s) 587, 830, 878
Hpy166II GTNNAC 4 cut(s) 601, 629, 836, 1054
Hpy188I TCNGA 8 cut(s) 139, 220, 264, 339, 743, 895, 942, 1005
Hpy188III TCNNGA 8 cut(s) 67, 172, 185, 287, 365, 521, 695, 980
Hpy8I GTNNAC 4 cut(s) 601, 629, 836, 1054
HpyAV CCTTC 4 cut(s) 274, 334, 1104, 1108
HpyCH4III ACNGT 1 cut(s) 721
HpyCH4IV ACGT 1 cut(s) 814
HpyCH4V TGCA 7 cut(s) 321, 391, 400, 554, 785, 854, 991
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 90, 397, 782, 1157
HpyF3I CTNAG 2 cut(s) 522, 740
HpySE526I ACGT 1 cut(s) 814
Hsp92I GRCGYC 1 cut(s) 685
Hsp92II CATG 5 cut(s) 28, 188, 587, 622, 854
HspAI GCGC 2 cut(s) 449, 774
Kpn2I TCCGGA 1 cut(s) 364
KroI GCCGGC 1 cut(s) 911
KroNI GCCGGC 1 cut(s) 913
Kzo9I GATC 5 cut(s) 174, 249, 259, 304, 1253
LmnI GCTCC 7 cut(s) 4, 57, 65, 140, 493, 974, 982
Lsp1109I GCAGC 3 cut(s) 44, 375, 769
LweI GCATC 3 cut(s) 495, 541, 1138
MaeI CTAG 3 cut(s) 381, 1121, 1251
MaeII ACGT 1 cut(s) 814
MaeIII GTNAC 2 cut(s) 1123, 1174
MalI GATC 5 cut(s) 176, 251, 261, 306, 1255
MboI GATC 5 cut(s) 174, 249, 259, 304, 1253
MboII GAAGA 9 cut(s) 25, 40, 43, 109, 233, 299, 1052, 1058, 1193
MfeI CAATTG 1 cut(s) 713
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 3 cut(s) 9, 302, 979
MluCI AATT 8 cut(s) 127, 533, 561, 654, 713, 930, 984, 1043
MlyI GAGTC 2 cut(s) 218, 1049
MmeI TCCRAC 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 856
MroI TCCGGA 1 cut(s) 364
MroNI GCCGGC 1 cut(s) 911
MseI TTAA 2 cut(s) 690, 723
MslI CAYNNNNRTG 2 cut(s) 405, 588
MspA1I CMGCKG 3 cut(s) 57, 733, 764
MspI CCGG 8 cut(s) 49, 132, 192, 213, 230, 256, 365, 912
MspR9I CCNGG 6 cut(s) 10, 214, 230, 256, 257, 405
MunI CAATTG 1 cut(s) 713
Mva1269I GAATGC 1 cut(s) 84
MvaI CCWGG 2 cut(s) 10, 405
MwoI GCNNNNNNNGC 5 cut(s) 49, 90, 397, 782, 1157
NaeI GCCGGC 1 cut(s) 913
NciI CCSGG 4 cut(s) 214, 230, 256, 257
NdeII GATC 5 cut(s) 174, 249, 259, 304, 1253
NgoMIV GCCGGC 1 cut(s) 911
NlaIII CATG 5 cut(s) 28, 188, 587, 622, 854
NmeAIII GCCGAG 4 cut(s) 69, 190, 232, 252
NmuCI GTSAC 1 cut(s) 1123
NsiI ATGCAT 1 cut(s) 856
OliI CACNNNNGTG 1 cut(s) 405
PagI TCATGA 1 cut(s) 184
PctI GAATGC 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 913
PfeI GAWTC 2 cut(s) 188, 751
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 4 cut(s) 22, 59, 390, 784
PleI GAGTC 2 cut(s) 217, 1049
PpsI GAGTC 2 cut(s) 217, 1049
PpuMI RGGWCCY 2 cut(s) 226, 1306
Psp124BI GAGCTC 1 cut(s) 979
Psp5II RGGWCCY 2 cut(s) 226, 1306
Psp6I CCWGG 2 cut(s) 8, 403
PspFI CCCAGC 2 cut(s) 1130, 1160
PspGI CCWGG 2 cut(s) 8, 403
PspOMI GGGCCC 1 cut(s) 298
PspPI GGNCC 9 cut(s) 42, 194, 216, 226, 298, 299, 836, 1106, 1306
PspPPI RGGWCCY 2 cut(s) 226, 1306
PsrI GAACNNNNNNTAC 4 cut(s) 318, 350, 720, 752
PsuI RGATCY 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 230
PvuII CAGCTG 2 cut(s) 733, 764
RsaI GTAC 3 cut(s) 327, 514, 738
RsaNI GTAC 3 cut(s) 326, 513, 737
RseI CAYNNNNRTG 2 cut(s) 405, 588
SacI GAGCTC 1 cut(s) 979
SalI GTCGAC 2 cut(s) 627, 1052
SaqAI TTAA 2 cut(s) 690, 723
SatI GCNGC 4 cut(s) 21, 58, 389, 783
Sau3AI GATC 5 cut(s) 174, 249, 259, 304, 1253
Sau96I GGNCC 9 cut(s) 42, 194, 216, 226, 298, 299, 836, 1106, 1306
SchI GAGTC 2 cut(s) 218, 1049
ScrFI CCNGG 6 cut(s) 10, 214, 230, 256, 257, 405
SduI GDGCHC 3 cut(s) 9, 302, 979
SfaNI GCATC 3 cut(s) 495, 541, 1138
SfcI CTRYAG 2 cut(s) 777, 1310
SinI GGWCC 5 cut(s) 216, 226, 836, 1106, 1306
SmaI CCCGGG 1 cut(s) 257
SmiMI CAYNNNNRTG 2 cut(s) 405, 588
SmlI CTYRAG 2 cut(s) 239, 488
SmoI CTYRAG 2 cut(s) 239, 488
Sse9I AATT 8 cut(s) 127, 533, 561, 654, 713, 930, 984, 1043
SsiI CCGC 6 cut(s) 21, 37, 55, 112, 150, 1151
SspMI CTAG 3 cut(s) 381, 1121, 1251
SstI GAGCTC 1 cut(s) 979
StyD4I CCNGG 6 cut(s) 8, 212, 228, 254, 255, 403
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 721
TaiI ACGT 1 cut(s) 817
TaqI TCGA 7 cut(s) 144, 288, 350, 628, 876, 907, 1053
TasI AATT 8 cut(s) 127, 533, 561, 654, 713, 930, 984, 1043
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 2 cut(s) 188, 751
Tru1I TTAA 2 cut(s) 690, 723
Tru9I TTAA 2 cut(s) 690, 723
TscAI CASTG 1 cut(s) 1132
TseFI GTSAC 1 cut(s) 1123
TseI GCWGC 3 cut(s) 57, 388, 782
Tsp45I GTSAC 1 cut(s) 1123
TspDTI ATGAA 4 cut(s) 41, 201, 858, 1090
TspGWI ACGGA 3 cut(s) 52, 300, 470
TspMI CCCGGG 1 cut(s) 255
TspRI CASTG 1 cut(s) 1132
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 5 cut(s) 216, 226, 836, 1106, 1306
XapI RAATTY 2 cut(s) 561, 1043
XmaI CCCGGG 1 cut(s) 255
XmiI GTMKAC 2 cut(s) 628, 1053
XspI CTAG 3 cut(s) 381, 1121, 1251
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.