pycom13g03410

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001016
Physical Location & Seq
Forward (+)
2261671 .. 2264132
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03410.3

Sequence Viewer

Length: 1317 bp
ATGGAGCCGCCGTGGGACAAGAGCATGAAGAAGAAGCGGAAGCGGAGCCGCCGCCCCGTCAGGCTGAGATGCTCCGTCAAGAATTACGATTGGGGCATTGTCGGCCGCAATTCCAAGGTCGCCAGGCTTTTCGCGCTCAATTCCGGGTCTGACATCGACCCGGGCAAGCCGTACGCGGAGTTCTGGATTGGGACTCATGAATCTGGGCCGTCGTATGCGGATTCCGGGTCGGAGCCCTTGAGTCTCAAGGCCTGGATCTCGCAGGATCCTGGTGTGCTTGGAGACAAGGTGGTTGAAAAATGGGGTGCTGATCTTCCGTTTTTGTTTAAGGTGCTTTCGGTTGGGAAGGCGTTGTCGATACAGGCTCATCCGGATAAAGAACTGGCTAGGGTGTTGCACAAGTTACACCCAAGTGTTTTCCGAGATGACAATCACAAGCCTGAAATGGCATTGGCATTGACGGAATTCGAGGCTCTTTGTGGGTTTATCAGTGTCAAGGAGCTTAAGGACATGCTTAATAGTGTTCCCGAGATTGCAGAATTGGTTGTGGTTGGAGATGCAGAGCGGATTCTGCTTGGTAGTGAGCACCATGAGAATGGGAAAGCCAAAACGGATATAGAGTCCATCTTCACTCGACTTATGTTACTAAGTAAAGATACAATTGCTGAGATGATTTCTAGACTGAAAAGACGCCTTTATCTAGAGAAAAAGAAAAGGCATTTGACGGTTAAAGAACAACTGGTACTCAGATTGGAGAGTCAATATCCAGATGATGTGGGTGCAATAGCAGCGTTTTTTCTTAACTATGTGAAGCTGAACCGTGGTGAAGCAATAAGTCTTGGGCCAAATGAACCTCATGCATATATTTCTGGTGAATGCATTGAGTGTATGGCAACTTCTGACAATGTTGTTCGTGCTGGCCTTACCTCAAAACCATTGGATGTCCAAACACTTCTTTCTATGCTCGAATACAGACAGGGTTCTCCTGAAATTATGCAAGGCGTTTCTTTGAATCCATACACAACAAGGTATCTCCCACCTTTTGAAGAATTTGAAGTTGACTGCTGTAACCTTCCTCACTCTGCATCGGTGGTTTTTCCTTCAGTCCTAGGACCATCCCTCTTCCTGTTTACTGCCGGGAAAGGACATTTTGATGCAAGTCTGTCTGAGGATGATATAGTGACCGGTGATGATATAGTTGAAGAGGGTGAGGTTTTCTTTGTGCCTGCCAACACGAAGATTAGCATTACCGCCAAATCAACAGAACTACAATTGTATAGGGTGGGAGTGAACAGCAGAATATTTAGGGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

439

Amino Acids

48.92

Weight (kDa)

6.17

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 565
AccII CGCG 2 cut(s) 134, 176
AccIII TCCGGA 1 cut(s) 370
AclWI GGATC 3 cut(s) 260, 263, 273
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 464, 1049
AcuI CTGAAG 1 cut(s) 1086
AcyI GRCGYC 1 cut(s) 691
AfaI GTAC 2 cut(s) 173, 744
AflII CTTAAG 1 cut(s) 503
AgeI ACCGGT 1 cut(s) 1184
AgsI TTSAA 5 cut(s) 296, 1012, 1046, 1055, 1202
AjnI CCWGG 3 cut(s) 122, 251, 268
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 2 cut(s) 502, 814
AluI AGCT 2 cut(s) 502, 814
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 2 cut(s) 248, 276
AlwI GGATC 3 cut(s) 260, 263, 273
Ama87I CYCGRG 2 cut(s) 160, 527
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 5 cut(s) 103, 206, 249, 842, 919
ApeKI GCWGC 1 cut(s) 788
ApoI RAATTY 2 cut(s) 464, 1049
AsiGI ACCGGT 1 cut(s) 1184
AspA2I CCTAGG 1 cut(s) 1108
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 3 cut(s) 206, 842, 1112
AsuC2I CCSGG 5 cut(s) 145, 161, 162, 226, 1138
AsuHPI GGTGA 4 cut(s) 836, 884, 1199, 1220
AvaI CYCGRG 2 cut(s) 160, 527
AvaII GGWCC 1 cut(s) 1112
AvrII CCTAGG 1 cut(s) 1108
BamHI GGATCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 880, 912
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 800
BccI CCATC 2 cut(s) 632, 1123
BceAI ACGGC 2 cut(s) 154, 193
BciT130I CCWGG 3 cut(s) 124, 253, 270
BcnI CCSGG 5 cut(s) 145, 161, 162, 226, 1138
BcoDI GTCTC 2 cut(s) 248, 276
BfaI CTAG 4 cut(s) 387, 678, 701, 1109
BfrI CTTAAG 1 cut(s) 503
BisI GCNGC 5 cut(s) 8, 49, 52, 106, 789
BlnI CCTAGG 1 cut(s) 1108
BlsI GCNGC 5 cut(s) 9, 50, 53, 107, 790
Bme1390I CCNGG 8 cut(s) 124, 145, 161, 162, 226, 253, 270, 1138
Bme18I GGWCC 1 cut(s) 1112
BmeT110I CYCGRG 2 cut(s) 160, 527
BmgT120I GGNCC 3 cut(s) 206, 842, 1112
BmiI GGNNCC 4 cut(s) 6, 47, 234, 267
BmrFI CCNGG 8 cut(s) 124, 145, 161, 162, 226, 253, 270, 1138
BmsI GCATC 4 cut(s) 59, 547, 1094, 1144
BpuEI CTTGAG 2 cut(s) 230, 259
BpuMI CCSGG 5 cut(s) 145, 161, 162, 226, 1138
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 691
BsaJI CCNNGG 5 cut(s) 11, 114, 160, 820, 1108
BsaWI WCCGGW 2 cut(s) 370, 1184
BsaXI ACNNNNNCTCC 3 cut(s) 26, 273, 303
Bse118I RCCGGY 1 cut(s) 1184
Bse1I ACTGG 2 cut(s) 387, 744
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 3 cut(s) 124, 253, 270
BseDI CCNNGG 5 cut(s) 11, 114, 160, 820, 1108
BseGI GGATG 4 cut(s) 367, 946, 1115, 1177
BseJI GATNNNNATC 1 cut(s) 429
BseMII CTCAG 4 cut(s) 56, 657, 760, 1158
BseNI ACTGG 2 cut(s) 387, 744
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 1 cut(s) 800
Bsh1236I CGCG 2 cut(s) 134, 176
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 5 cut(s) 105, 208, 251, 844, 921
BshTI ACCGGT 1 cut(s) 1184
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 2 cut(s) 160, 527
BsiSI CCGG 6 cut(s) 144, 161, 225, 371, 1137, 1185
BsiWI CGTACG 1 cut(s) 171
BslFI GGGAC 2 cut(s) 29, 205
BsmAI GTCTC 2 cut(s) 248, 276
BsmFI GGGAC 2 cut(s) 29, 205
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 5 cut(s) 105, 208, 251, 844, 921
BsoBI CYCGRG 2 cut(s) 160, 527
Bsp1286I GDGCHC 2 cut(s) 237, 588
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 3 cut(s) 255, 265, 310
BspANI GGCC 5 cut(s) 105, 208, 251, 844, 921
BspCNI CTCAG 4 cut(s) 57, 658, 759, 1159
BspEI TCCGGA 1 cut(s) 370
BspFNI CGCG 2 cut(s) 134, 176
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 4 cut(s) 6, 47, 234, 267
BspPI GGATC 3 cut(s) 260, 263, 273
BspTI CTTAAG 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 565
BsrFI RCCGGY 1 cut(s) 1184
BsrI ACTGG 2 cut(s) 387, 744
BssAI RCCGGY 1 cut(s) 1184
BssECI CCNNGG 5 cut(s) 11, 114, 160, 820, 1108
BssMI GATC 3 cut(s) 255, 265, 310
BssNI GRCGYC 1 cut(s) 691
BssT1I CCWWGG 2 cut(s) 114, 1108
Bst2UI CCWGG 3 cut(s) 124, 253, 270
Bst4CI ACNGT 2 cut(s) 727, 821
Bst6I CTCTTC 2 cut(s) 1127, 1197
BstACI GRCGYC 1 cut(s) 691
BstAFI CTTAAG 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 167, 919, 1227
BstDEI CTNAG 5 cut(s) 65, 647, 666, 746, 1167
BstDSI CCRYGG 2 cut(s) 11, 820
BstF5I GGATG 4 cut(s) 367, 946, 1115, 1177
BstFNI CGCG 2 cut(s) 134, 176
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 3 cut(s) 258, 268, 313
BstMAI GTCTC 2 cut(s) 248, 276
BstMBI GATC 3 cut(s) 255, 265, 310
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 4 cut(s) 102, 133, 571, 788
BstNI CCWGG 3 cut(s) 124, 253, 270
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 8 cut(s) 122, 143, 159, 160, 224, 251, 268, 1136
BstUI CGCG 2 cut(s) 134, 176
BstV1I GCAGC 1 cut(s) 800
BstX2I RGATCY 2 cut(s) 255, 265
BstXI CCANNNNNNTGG 1 cut(s) 596
BstYI RGATCY 2 cut(s) 255, 265
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 5 cut(s) 105, 208, 251, 844, 921
BtgI CCRYGG 2 cut(s) 11, 820
BtsCI GGATG 4 cut(s) 367, 946, 1115, 1177
BtsIMutI CAGTG 1 cut(s) 496
Cac8I GCNNGC 3 cut(s) 167, 919, 1227
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 136
Cfr10I RCCGGY 1 cut(s) 1184
Cfr13I GGNCC 3 cut(s) 206, 842, 1112
Cfr9I CCCGGG 1 cut(s) 160
CseI GACGC 1 cut(s) 699
Csp6I GTAC 2 cut(s) 172, 743
CspAI ACCGGT 1 cut(s) 1184
CviAII CATG 5 cut(s) 25, 197, 511, 590, 857
CviQI GTAC 2 cut(s) 172, 743
DdeI CTNAG 5 cut(s) 65, 647, 666, 746, 1167
DpnI GATC 3 cut(s) 257, 267, 312
DpnII GATC 3 cut(s) 255, 265, 310
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 1127, 1197
EarI CTCTTC 2 cut(s) 1127, 1197
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 114, 1108
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 1112
Eco52I CGGCCG 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 1086
Eco88I CYCGRG 2 cut(s) 160, 527
EcoRI GAATTC 1 cut(s) 464
EcoRII CCWGG 3 cut(s) 122, 251, 268
EcoT14I CCWWGG 2 cut(s) 114, 1108
EcoT22I ATGCAT 2 cut(s) 862, 881
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 2 cut(s) 114, 1108
FaeI CATG 5 cut(s) 28, 200, 514, 593, 860
FaqI GGGAC 2 cut(s) 29, 205
FatI CATG 5 cut(s) 24, 196, 510, 589, 856
Fnu4HI GCNGC 5 cut(s) 8, 49, 52, 106, 789
FokI GGATG 4 cut(s) 354, 953, 1102, 1184
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 8, 49, 52, 106, 789
FspBI CTAG 4 cut(s) 387, 678, 701, 1109
GlaI GCGC 1 cut(s) 135
GluI GCNGC 5 cut(s) 8, 49, 52, 106, 789
HaeIII GGCC 5 cut(s) 105, 208, 251, 844, 921
HapII CCGG 6 cut(s) 144, 161, 225, 371, 1137, 1185
HgaI GACGC 1 cut(s) 699
HhaI GCGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 691
Hin1II CATG 5 cut(s) 28, 200, 514, 593, 860
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HinfI GANTC 8 cut(s) 193, 200, 221, 241, 568, 620, 757, 1012
HpaII CCGG 6 cut(s) 144, 161, 225, 371, 1137, 1185
HphI GGTGA 4 cut(s) 836, 884, 1199, 1220
Hpy166II GTNNAC 3 cut(s) 1060, 1131, 1291
Hpy188I TCNGA 6 cut(s) 151, 232, 422, 749, 901, 1168
Hpy188III TCNNGA 9 cut(s) 79, 184, 197, 371, 527, 678, 701, 767, 986
Hpy8I GTNNAC 3 cut(s) 1060, 1131, 1291
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 3 cut(s) 340, 1082, 1110
HpyCH4III ACNGT 2 cut(s) 727, 821
HpyCH4V TGCA 9 cut(s) 397, 536, 560, 782, 860, 879, 997, 1085, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 102, 133, 571, 788
HpyF3I CTNAG 5 cut(s) 65, 647, 666, 746, 1167
Hsp92I GRCGYC 1 cut(s) 691
Hsp92II CATG 5 cut(s) 28, 200, 514, 593, 860
HspAI GCGC 1 cut(s) 134
Kpn2I TCCGGA 1 cut(s) 370
Kzo9I GATC 3 cut(s) 255, 265, 310
LmnI GCTCC 5 cut(s) 4, 45, 77, 232, 499
Lsp1109I GCAGC 1 cut(s) 800
LweI GCATC 4 cut(s) 59, 547, 1094, 1144
MaeI CTAG 4 cut(s) 387, 678, 701, 1109
MaeIII GTNAC 4 cut(s) 402, 642, 1067, 1180
MalI GATC 3 cut(s) 257, 267, 312
MbiI CCGCTC 1 cut(s) 565
MboI GATC 3 cut(s) 255, 265, 310
MboII GAAGA 8 cut(s) 40, 43, 305, 619, 1058, 1114, 1214, 1249
MfeI CAATTG 2 cut(s) 660, 1271
MflI RGATCY 2 cut(s) 255, 265
MhlI GDGCHC 2 cut(s) 237, 588
MluCI AATT 9 cut(s) 82, 109, 139, 464, 539, 660, 990, 1049, 1271
MlyI GAGTC 4 cut(s) 187, 250, 629, 766
MmeI TCCRAC 2 cut(s) 210, 532
MnlI CCTC 8 cut(s) 463, 864, 937, 1086, 1130, 1162, 1198, 1204
Mph1103I ATGCAT 2 cut(s) 862, 881
MroI TCCGGA 1 cut(s) 370
MseI TTAA 5 cut(s) 327, 504, 516, 729, 801
MslI CAYNNNNRTG 3 cut(s) 411, 594, 1152
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 6 cut(s) 144, 161, 225, 371, 1137, 1185
MspR9I CCNGG 8 cut(s) 124, 145, 161, 162, 226, 253, 270, 1138
MunI CAATTG 2 cut(s) 660, 1271
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 3 cut(s) 124, 253, 270
MvnI CGCG 2 cut(s) 134, 176
MwoI GCNNNNNNNGC 4 cut(s) 102, 133, 571, 788
NciI CCSGG 5 cut(s) 145, 161, 162, 226, 1138
NdeII GATC 3 cut(s) 255, 265, 310
NlaIII CATG 5 cut(s) 28, 200, 514, 593, 860
NlaIV GGNNCC 4 cut(s) 6, 47, 234, 267
NmuCI GTSAC 1 cut(s) 1180
NsiI ATGCAT 2 cut(s) 862, 881
NspI RCATGY 1 cut(s) 514
OliI CACNNNNGTG 1 cut(s) 411
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 251
PctI GAATGC 1 cut(s) 881
PfeI GAWTC 4 cut(s) 200, 221, 568, 1012
Pfl23II CGTACG 1 cut(s) 171
PinAI ACCGGT 1 cut(s) 1184
PkrI GCNGC 5 cut(s) 9, 50, 53, 107, 790
PleI GAGTC 4 cut(s) 187, 249, 628, 765
PpsI GAGTC 4 cut(s) 187, 249, 628, 765
Psp6I CCWGG 3 cut(s) 122, 251, 268
PspGI CCWGG 3 cut(s) 122, 251, 268
PspLI CGTACG 1 cut(s) 171
PspN4I GGNNCC 4 cut(s) 6, 47, 234, 267
PspPI GGNCC 3 cut(s) 206, 842, 1112
PsrI GAACNNNNNNTAC 4 cut(s) 164, 196, 726, 758
PsuI RGATCY 2 cut(s) 255, 265
RsaI GTAC 2 cut(s) 173, 744
RsaNI GTAC 2 cut(s) 172, 743
RseI CAYNNNNRTG 3 cut(s) 411, 594, 1152
SaqAI TTAA 5 cut(s) 327, 504, 516, 729, 801
SatI GCNGC 5 cut(s) 8, 49, 52, 106, 789
Sau3AI GATC 3 cut(s) 255, 265, 310
Sau96I GGNCC 3 cut(s) 206, 842, 1112
SchI GAGTC 4 cut(s) 187, 250, 629, 766
ScrFI CCNGG 8 cut(s) 124, 145, 161, 162, 226, 253, 270, 1138
SduI GDGCHC 2 cut(s) 237, 588
SfaNI GCATC 4 cut(s) 59, 547, 1094, 1144
SinI GGWCC 1 cut(s) 1112
SmaI CCCGGG 1 cut(s) 162
SmiMI CAYNNNNRTG 3 cut(s) 411, 594, 1152
SmlI CTYRAG 3 cut(s) 238, 245, 503
SmoI CTYRAG 3 cut(s) 238, 245, 503
Sse9I AATT 9 cut(s) 82, 109, 139, 464, 539, 660, 990, 1049, 1271
SseBI AGGCCT 1 cut(s) 251
SspI AATATT 1 cut(s) 1302
SspMI CTAG 4 cut(s) 387, 678, 701, 1109
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 8 cut(s) 122, 143, 159, 160, 224, 251, 268, 1136
StyI CCWWGG 2 cut(s) 114, 1108
TaaI ACNGT 2 cut(s) 727, 821
TaqI TCGA 5 cut(s) 156, 356, 468, 634, 966
TasI AATT 9 cut(s) 82, 109, 139, 464, 539, 660, 990, 1049, 1271
TauI GCSGC 4 cut(s) 10, 51, 54, 108
TfiI GAWTC 4 cut(s) 200, 221, 568, 1012
Tru1I TTAA 5 cut(s) 327, 504, 516, 729, 801
Tru9I TTAA 5 cut(s) 327, 504, 516, 729, 801
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 1180
TseI GCWGC 1 cut(s) 788
Tsp45I GTSAC 1 cut(s) 1180
TspDTI ATGAA 3 cut(s) 41, 213, 864
TspGWI ACGGA 4 cut(s) 64, 306, 476, 626
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 1 cut(s) 496
Vha464I CTTAAG 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 1112
XapI RAATTY 2 cut(s) 464, 1049
XbaI TCTAGA 2 cut(s) 677, 700
XceI RCATGY 1 cut(s) 514
XmaI CCCGGG 1 cut(s) 160
XmaJI CCTAGG 1 cut(s) 1108
XspI CTAG 4 cut(s) 387, 678, 701, 1109
Zsp2I ATGCAT 2 cut(s) 862, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.