Rw4G007710

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001016
Physical Location & Seq
Reverse (-)
16587379 .. 16588864
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007710.1

Sequence Viewer

Length: 1209 bp
ATGGAGATGCAACAACCAAAGCAGAGGCAGAGGAGTGTTCAGAGGCTAAGATGCTCTGTCATGAACTACGACTGGGGTAAGAAAGGCCAAGACTCTGTGGTGGGCAGGTTGTGTGCCTCCAATTCTCATTCTGAAATCGACCTAGAAAAGCCTTACGCTGAAATGTGGATGGGAACACATGACTCTGGACCCTCCTTTTTGATTGATCAGGATCTGAATAATAATAATAGCACCACTACTGGATCATCTCCGTCTCTCAGAGACTGGGTTTCTAACAACCCTAATGACATGCTCGGTCACAAGGTTGTCCAAAAATGGGGCTCCTCTGATCTGCCTTTCTTGTTTAAGGTGCAATCCGTGGCAAAGGCATTGTCAATACAGGCACACCCAGATAAGGAATTGGCCAAGGCTTTGCACAAGTCTATGCCAGATATTTACAAGGATGATAATTATAAGCCTGAGATGGCGCTGGCCTTGACACATTTTCAGGCTCTTTGTGGTTTTATCACTCTTGAGGATGAGTTCGAAAACCAAGTGCTTCGCACGACCAAAGAAGATGGGGAGGATGAAGTCAAGTCTGTTATACGTTTGATTTTTACCCAACTCATGTCAACTAACAAGGAAATTATGACCACAGTAACAACTAAATTGAAGAGTCGTTTACAGACGGAAAGCCAGGTGAGGCAGCTGACAGAAAAGGAGCAGCTAGTGTTGCAATTGGAAAAGCAATATCCGAACGATGTGGGTGTGATATCTGCATTCTTTCTTAACCATGTGAAGCTTAATCCTGGAGAAGCATTATGCCTTGGAGCAAATGAACCCCATGCCTACATATCTGGGGATTGCATCGAGTGCATGGCAACCTCAGACAACGTAGTTAGGGCTGGCCTTACTCCCAAGCATCGAGATATCAAAACCCTTTTTGCTGTGAATTCTCATGTAACAAGGTACATACCGCCTTTTGAAGAATTTGAGGTTGATCGGATCCATCTTCCCCACGGAGAATCAACAGAGTTTCCTGCAGTACCTGGTCCTTCCATTTTTGTGGTCACTTTTGGGAAGGGAACCATACGTACGAGTAATGGAGATCATGCAATCATCCAAGGAGATGTTCTGTTTGCACCTGCACACACTGAGATTAGCATAACAAGTGCATCTGAGTTGCACATATATAGAGCCGGAGTGAATAGCACATTCTTAGCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

402

Amino Acids

44.95

Weight (kDa)

5.84

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 13 - 168 1.6e-43 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 174 - 256 1e-13 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AarI CACCTGC 1 cut(s) 1132
Acc36I ACCTGC 2 cut(s) 96, 1132
AciI CCGC 1 cut(s) 956
AclWI GGATC 4 cut(s) 219, 250, 979, 992
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 2 cut(s) 931, 968
AfaI GTAC 3 cut(s) 950, 1026, 1075
AfiI CCNNNNNNNGG 2 cut(s) 316, 394
AgsI TTSAA 2 cut(s) 652, 965
AjnI CCWGG 3 cut(s) 675, 787, 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 1095, 1127
AluBI AGCT 3 cut(s) 688, 706, 781
AluI AGCT 3 cut(s) 688, 706, 781
Alw26I GTCTC 2 cut(s) 255, 258
AlwI GGATC 4 cut(s) 219, 250, 979, 992
AlwNI CAGNNNCTG 3 cut(s) 214, 264, 1028
AoxI GGCC 4 cut(s) 85, 402, 471, 886
ApeKI GCWGC 2 cut(s) 685, 703
ApoI RAATTY 2 cut(s) 931, 968
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AspLEI GCGC 1 cut(s) 469
AspS9I GGNCC 2 cut(s) 188, 1031
AsuHPI GGTGA 1 cut(s) 691
AsuII TTCGAA 1 cut(s) 525
AvaII GGWCC 2 cut(s) 188, 1031
BalI TGGCCA 1 cut(s) 404
BamHI GGATCC 1 cut(s) 984
BanII GRGCYC 1 cut(s) 323
BbvI GCAGC 2 cut(s) 697, 715
BccI CCATC 4 cut(s) 163, 457, 551, 996
BciT130I CCWGG 3 cut(s) 677, 789, 1029
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 255, 258
BfaI CTAG 2 cut(s) 143, 707
BfmI CTRYAG 1 cut(s) 1020
BfoI RGCGCY 1 cut(s) 470
BfuAI ACCTGC 2 cut(s) 96, 1132
BisI GCNGC 2 cut(s) 686, 704
BlsI GCNGC 2 cut(s) 687, 705
Bme1390I CCNGG 3 cut(s) 677, 789, 1029
Bme18I GGWCC 2 cut(s) 188, 1031
BmgT120I GGNCC 2 cut(s) 188, 1031
BmiI GGNNCC 4 cut(s) 190, 322, 986, 1066
BmrFI CCNGG 3 cut(s) 677, 789, 1029
BmrI ACTGGG 2 cut(s) 82, 274
BmsI GCATC 4 cut(s) 41, 855, 910, 1163
BmuI ACTGGG 2 cut(s) 82, 274
BpmI CTGGAG 1 cut(s) 810
Bpu14I TTCGAA 1 cut(s) 525
BpuEI CTTGAG 1 cut(s) 533
BsaAI YACGTR 1 cut(s) 1073
BsaBI GATNNNNATC 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 357, 405, 805, 997, 1102
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 394
Bse1I ACTGG 3 cut(s) 77, 244, 269
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 3 cut(s) 677, 789, 1029
BseDI CCNNGG 5 cut(s) 357, 405, 805, 997, 1102
BseGI GGATG 5 cut(s) 174, 448, 523, 571, 1098
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 2 cut(s) 316, 394
BseMII CTCAG 5 cut(s) 271, 450, 879, 1125, 1149
BseNI ACTGG 3 cut(s) 77, 244, 269
BseRI GAGGAG 2 cut(s) 46, 313
BseXI GCAGC 2 cut(s) 697, 715
BsgI GTGCAG 1 cut(s) 1110
BshFI GGCC 4 cut(s) 87, 404, 473, 888
BsiSI CCGG 1 cut(s) 1179
BsiWI CGTACG 1 cut(s) 1073
BslI CCNNNNNNNGG 2 cut(s) 316, 394
BsmAI GTCTC 2 cut(s) 255, 258
BsmBI CGTCTC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 758
BsnI GGCC 4 cut(s) 87, 404, 473, 888
Bsp119I TTCGAA 1 cut(s) 525
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
BspACI CCGC 1 cut(s) 956
BspANI GGCC 4 cut(s) 87, 404, 473, 888
BspCNI CTCAG 5 cut(s) 270, 451, 878, 1126, 1150
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 4 cut(s) 190, 322, 986, 1066
BspMAI CTGCAG 1 cut(s) 1024
BspMI ACCTGC 2 cut(s) 96, 1132
BspPI GGATC 4 cut(s) 219, 250, 979, 992
BspT104I TTCGAA 1 cut(s) 525
BsrI ACTGG 3 cut(s) 77, 244, 269
BssECI CCNNGG 5 cut(s) 357, 405, 805, 997, 1102
BssMI GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
BssT1I CCWWGG 3 cut(s) 405, 805, 1102
Bst2UI CCWGG 3 cut(s) 677, 789, 1029
Bst4CI ACNGT 1 cut(s) 637
Bst6I CTCTTC 1 cut(s) 647
BstAPI GCANNNNNTGC 1 cut(s) 852
BstBAI YACGTR 1 cut(s) 1073
BstBI TTCGAA 1 cut(s) 525
BstC8I GCNNGC 2 cut(s) 471, 886
BstDEI CTNAG 7 cut(s) 47, 257, 459, 865, 1134, 1158, 1198
BstDSI CCRYGG 2 cut(s) 357, 997
BstF5I GGATG 5 cut(s) 174, 448, 523, 571, 1098
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 1 cut(s) 469
BstKTI GATC 7 cut(s) 208, 214, 245, 331, 982, 987, 1090
BstMAI GTCTC 2 cut(s) 255, 258
BstMBI GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
BstMWI GCNNNNNNNGC 2 cut(s) 712, 852
BstNI CCWGG 3 cut(s) 677, 789, 1029
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 675, 787, 1027
BstSFI CTRYAG 1 cut(s) 1020
BstSNI TACGTA 1 cut(s) 1073
BstV1I GCAGC 2 cut(s) 697, 715
BstX2I RGATCY 2 cut(s) 211, 984
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 2 cut(s) 211, 984
BsuRI GGCC 4 cut(s) 87, 404, 473, 888
BtgI CCRYGG 2 cut(s) 357, 997
BtsCI GGATG 5 cut(s) 174, 448, 523, 571, 1098
BtsIMutI CAGTG 1 cut(s) 1131
BveI ACCTGC 2 cut(s) 96, 1132
Cac8I GCNNGC 2 cut(s) 471, 886
CaiI CAGNNNCTG 3 cut(s) 214, 264, 1028
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 469
Cfr13I GGNCC 2 cut(s) 188, 1031
CsiI ACCWGGT 1 cut(s) 1027
Csp6I GTAC 3 cut(s) 949, 1025, 1074
CviAII CATG 9 cut(s) 61, 179, 289, 607, 773, 824, 856, 938, 1091
CviQI GTAC 3 cut(s) 949, 1025, 1074
DdeI CTNAG 7 cut(s) 47, 257, 459, 865, 1134, 1158, 1198
DpnI GATC 7 cut(s) 207, 213, 244, 330, 981, 986, 1089
DpnII GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
Eco105I TACGTA 1 cut(s) 1073
Eco130I CCWWGG 3 cut(s) 405, 805, 1102
Eco24I GRGCYC 1 cut(s) 323
Eco32I GATATC 2 cut(s) 753, 910
Eco47I GGWCC 2 cut(s) 188, 1031
EcoRI GAATTC 1 cut(s) 931
EcoRII CCWGG 3 cut(s) 675, 787, 1027
EcoRV GATATC 2 cut(s) 753, 910
EcoT14I CCWWGG 3 cut(s) 405, 805, 1102
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 3 cut(s) 405, 805, 1102
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 9 cut(s) 64, 182, 292, 610, 776, 827, 859, 941, 1094
FatI CATG 9 cut(s) 60, 178, 288, 606, 772, 823, 855, 937, 1090
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 686, 704
FokI GGATG 5 cut(s) 181, 455, 530, 578, 1085
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 686, 704
FspBI CTAG 2 cut(s) 143, 707
GlaI GCGC 1 cut(s) 468
GluI GCNGC 2 cut(s) 686, 704
GsuI CTGGAG 1 cut(s) 810
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 4 cut(s) 87, 404, 473, 888
HapII CCGG 1 cut(s) 1179
HhaI GCGC 1 cut(s) 469
Hin1II CATG 9 cut(s) 64, 182, 292, 610, 776, 827, 859, 941, 1094
Hin6I GCGC 1 cut(s) 467
HinP1I GCGC 1 cut(s) 467
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 4 cut(s) 92, 182, 655, 1004
HpaII CCGG 1 cut(s) 1179
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 2 cut(s) 612, 662
Hpy188I TCNGA 9 cut(s) 42, 133, 216, 260, 328, 735, 868, 984, 1159
Hpy188III TCNNGA 5 cut(s) 61, 186, 209, 512, 905
Hpy8I GTNNAC 2 cut(s) 612, 662
HpyAV CCTTC 2 cut(s) 1044, 1054
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4IV ACGT 3 cut(s) 586, 873, 1072
HpyF10VI GCNNNNNNNGC 2 cut(s) 712, 852
HpyF3I CTNAG 7 cut(s) 47, 257, 459, 865, 1134, 1158, 1198
HpySE526I ACGT 3 cut(s) 586, 873, 1072
Hsp92II CATG 9 cut(s) 64, 182, 292, 610, 776, 827, 859, 941, 1094
HspAI GCGC 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
LmnI GCTCC 3 cut(s) 326, 700, 809
Lsp1109I GCAGC 2 cut(s) 697, 715
LweI GCATC 4 cut(s) 41, 855, 910, 1163
MabI ACCWGGT 1 cut(s) 1027
MaeI CTAG 2 cut(s) 143, 707
MaeII ACGT 3 cut(s) 586, 873, 1072
MaeIII GTNAC 4 cut(s) 296, 637, 940, 1048
MalI GATC 7 cut(s) 207, 213, 244, 330, 981, 986, 1089
MboI GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
MboII GAAGA 4 cut(s) 566, 664, 977, 983
MfeI CAATTG 1 cut(s) 716
MflI RGATCY 2 cut(s) 211, 984
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 8 cut(s) 121, 398, 448, 624, 647, 716, 931, 968
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 3 cut(s) 86, 176, 664
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
MseI TTAA 3 cut(s) 345, 768, 783
MslI CAYNNNNRTG 1 cut(s) 1043
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 1 cut(s) 1179
MspR9I CCNGG 3 cut(s) 677, 789, 1029
MunI CAATTG 1 cut(s) 716
Mva1269I GAATGC 1 cut(s) 758
MvaI CCWGG 3 cut(s) 677, 789, 1029
MwoI GCNNNNNNNGC 2 cut(s) 712, 852
NdeII GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
NlaIII CATG 9 cut(s) 64, 182, 292, 610, 776, 827, 859, 941, 1094
NlaIV GGNNCC 4 cut(s) 190, 322, 986, 1066
NmuCI GTSAC 2 cut(s) 296, 1048
NspI RCATGY 1 cut(s) 292
NspV TTCGAA 1 cut(s) 525
PagI TCATGA 1 cut(s) 60
PaqCI CACCTGC 1 cut(s) 1132
PctI GAATGC 1 cut(s) 758
PfeI GAWTC 1 cut(s) 1004
Pfl23II CGTACG 1 cut(s) 1073
PfoI TCCNGGA 1 cut(s) 787
PkrI GCNGC 2 cut(s) 687, 705
PleI GAGTC 3 cut(s) 86, 176, 663
PpsI GAGTC 3 cut(s) 86, 176, 663
Ppu21I YACGTR 1 cut(s) 1073
PsiI TTATAA 1 cut(s) 453
Psp6I CCWGG 3 cut(s) 675, 787, 1027
PspGI CCWGG 3 cut(s) 675, 787, 1027
PspLI CGTACG 1 cut(s) 1073
PspN4I GGNNCC 4 cut(s) 190, 322, 986, 1066
PspPI GGNCC 2 cut(s) 188, 1031
PsrI GAACNNNNNNTAC 2 cut(s) 1057, 1089
PstI CTGCAG 1 cut(s) 1024
PstNI CAGNNNCTG 3 cut(s) 214, 264, 1028
PsuI RGATCY 2 cut(s) 211, 984
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 3 cut(s) 950, 1026, 1075
RsaNI GTAC 3 cut(s) 949, 1025, 1074
RseI CAYNNNNRTG 1 cut(s) 1043
SaqAI TTAA 3 cut(s) 345, 768, 783
SatI GCNGC 2 cut(s) 686, 704
Sau3AI GATC 7 cut(s) 205, 211, 242, 328, 979, 984, 1087
Sau96I GGNCC 2 cut(s) 188, 1031
SchI GAGTC 3 cut(s) 86, 176, 664
ScrFI CCNGG 3 cut(s) 677, 789, 1029
SduI GDGCHC 1 cut(s) 323
SexAI ACCWGGT 1 cut(s) 1027
SfaNI GCATC 4 cut(s) 41, 855, 910, 1163
SfcI CTRYAG 1 cut(s) 1020
SfuI TTCGAA 1 cut(s) 525
SinI GGWCC 2 cut(s) 188, 1031
SmiMI CAYNNNNRTG 1 cut(s) 1043
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SnaBI TACGTA 1 cut(s) 1073
Sse9I AATT 8 cut(s) 121, 398, 448, 624, 647, 716, 931, 968
SsiI CCGC 1 cut(s) 956
SspMI CTAG 2 cut(s) 143, 707
StyD4I CCNGG 3 cut(s) 675, 787, 1027
StyI CCWWGG 3 cut(s) 405, 805, 1102
TaaI ACNGT 1 cut(s) 637
TaiI ACGT 3 cut(s) 589, 876, 1075
TaqI TCGA 4 cut(s) 138, 525, 849, 904
TaqII GACCGA 1 cut(s) 284
TasI AATT 8 cut(s) 121, 398, 448, 624, 647, 716, 931, 968
TfiI GAWTC 1 cut(s) 1004
Tru1I TTAA 3 cut(s) 345, 768, 783
Tru9I TTAA 3 cut(s) 345, 768, 783
TscAI CASTG 1 cut(s) 1138
TseFI GTSAC 2 cut(s) 296, 1048
TseI GCWGC 2 cut(s) 685, 703
Tsp45I GTSAC 2 cut(s) 296, 1048
TspDTI ATGAA 3 cut(s) 77, 582, 831
TspGWI ACGGA 4 cut(s) 240, 346, 683, 1014
TspRI CASTG 1 cut(s) 1138
VpaK11BI GGWCC 2 cut(s) 188, 1031
XapI RAATTY 2 cut(s) 931, 968
XceI RCATGY 1 cut(s) 292
XspI CTAG 2 cut(s) 143, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.