Rroxscaffold_7G00161430

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001016
Physical Location & Seq
Reverse (-)
4192689 .. 4194288
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161430.1

Sequence Viewer

Length: 1002 bp
ATGGAGATGGAGAGGCAGAGGAGTGTTCAGAAGCTGAAATGCACTGTCCAGAACTACGAATGGGGAAAGAAAGGCCTAGATTCTGAGGTAGCAAGGCTTTGTGCCTCCAACTCTGGTAGTAAAATTGACCCACAAAAGCCTTACGCCGAGTTTTGGATGGGAACCCATCAATCTGGAACTTCCTTTTTGATTGATCCGAATAACACTACGTCGTCGTTGTCTCTCAAAGACTGGATCTCTGACAAACCAGATGAGATTCTTGGTCACAGGGTCGACGATAATCACAAGCCGGAGATGGCGCTCGCTACAACACATTTTCAAGCTCTTTGTGGTTTCATCGCTTTTCAGGAGCTTAAGGATGTGCTCAACAATGTACCCGAGATTGTGCAAGTGGTTGGAAGTGAAGTTGCAAACCAAGTGAGGCAGTTGATAGACAAGGAGCAGCTAGTGTTGCAATTGGAAGAGCAATATTCAGAAGAGAACGTGGGTGTGATATCTGCTTTCTTTCTCAACCATGTGAAGCTTAATCCTGGAGAAGCATTGTGCCTCGGAGCAAATGAACCTCATGCTTATGTATCCGGGGACTGCATTGAGTGCATGGCGACCTCGGACAACGTAGTCAGGGCTGGCCTTACTCCTAAGCATCGAGATGTTAAGACCCTTTGTTCTATGCTCACATATAAGCAGGGTTTCCCTGAAATCATGCGAGGAGTTGCTATGAATCCTTATGTGACAAGGTACATCCCTCCTTTCGATGAATTTGAGGTGGATCGGTGTCATCTTCCGGGTGGAGAATCGACGGAGTTTCCTGCAGTAACTGGTCCTTCAATTTTTGTGGTCACTCTTGGAAAGGGAACAATATGCACTAGTAACGGAGATAATAAAATTATCCAAGGAGACGTTCTGTTTGCATGTGCAGATACTCGGATTAGCGTAACGAGTGCATCTGAGTTGCATGTATATAGAGCCGGAGTGAACAGCATGGTCTTTGGGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

333

Amino Acids

36.71

Weight (kDa)

5.16

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 9 - 86 2.4e-15 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_C PF01238 247 - 289 3.8e-06 Phosphomannose isomerase type I C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
AccI GTMKAC 1 cut(s) 273
AclWI GGATC 3 cut(s) 188, 242, 777
AcsI RAATTY 1 cut(s) 758
AcyI GRCGYC 1 cut(s) 995
AfaI GTAC 2 cut(s) 375, 740
AfiI CCNNNNNNNGG 1 cut(s) 153
AflII CTTAAG 1 cut(s) 353
AgsI TTSAA 2 cut(s) 320, 828
AhlI ACTAGT 1 cut(s) 866
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 2 cut(s) 885, 917
AluBI AGCT 5 cut(s) 34, 323, 352, 445, 523
AluI AGCT 5 cut(s) 34, 323, 352, 445, 523
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 2 cut(s) 225, 891
AlwI GGATC 3 cut(s) 188, 242, 777
AlwNI CAGNNNCTG 2 cut(s) 34, 818
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 2 cut(s) 73, 628
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 1 cut(s) 758
ArsI GACNNNNNNTTYG 2 cut(s) 890, 922
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 1 cut(s) 821
AsuC2I CCSGG 2 cut(s) 580, 786
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 821
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 454
BccI CCATC 3 cut(s) 151, 174, 289
BciT130I CCWGG 1 cut(s) 531
BciVI GTATCC 1 cut(s) 586
BcnI CCSGG 2 cut(s) 580, 786
BcoDI GTCTC 2 cut(s) 225, 891
BcuI ACTAGT 1 cut(s) 866
BfaI CTAG 3 cut(s) 77, 446, 867
BfmI CTRYAG 1 cut(s) 810
BfoI RGCGCY 1 cut(s) 302
BfrI CTTAAG 1 cut(s) 353
BfuI GTATCC 1 cut(s) 586
BisI GCNGC 1 cut(s) 443
BlsI GCNGC 1 cut(s) 444
Bme1390I CCNGG 3 cut(s) 531, 580, 786
Bme18I GGWCC 1 cut(s) 821
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 3 cut(s) 531, 580, 786
BmsI GCATC 2 cut(s) 652, 953
BpmI CTGGAG 1 cut(s) 552
Bpu10I CCTNAGC 1 cut(s) 639
BpuMI CCSGG 2 cut(s) 580, 786
BsaHI GRCGYC 1 cut(s) 995
BsaJI CCNNGG 4 cut(s) 547, 579, 606, 892
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse1I ACTGG 2 cut(s) 236, 823
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 4 cut(s) 547, 579, 606, 892
BseGI GGATG 3 cut(s) 162, 364, 741
BseLI CCNNNNNNNGG 1 cut(s) 153
BseMII CTCAG 2 cut(s) 75, 939
BseNI ACTGG 2 cut(s) 236, 823
BseRI GAGGAG 2 cut(s) 34, 723
BseXI GCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 936
BshFI GGCC 2 cut(s) 75, 630
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 4 cut(s) 290, 579, 785, 969
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 153
BsmAI GTCTC 2 cut(s) 225, 891
BsmBI CGTCTC 1 cut(s) 891
BsmFI GGGAC 1 cut(s) 596
BsnI GGCC 2 cut(s) 75, 630
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 3 cut(s) 193, 234, 769
BspANI GGCC 2 cut(s) 75, 630
BspCNI CTCAG 2 cut(s) 76, 940
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 814
BspPI GGATC 3 cut(s) 188, 242, 777
BspQI GCTCTTC 1 cut(s) 456
BspTI CTTAAG 1 cut(s) 353
BsrI ACTGG 2 cut(s) 236, 823
BssECI CCNNGG 4 cut(s) 547, 579, 606, 892
BssMI GATC 3 cut(s) 193, 234, 769
BssNI GRCGYC 1 cut(s) 995
BssT1I CCWWGG 1 cut(s) 892
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 2 cut(s) 456, 471
BstACI GRCGYC 1 cut(s) 995
BstAFI CTTAAG 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 303, 628
BstDEI CTNAG 3 cut(s) 84, 639, 948
BstF5I GGATG 3 cut(s) 162, 364, 741
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 3 cut(s) 196, 237, 772
BstMAI GTCTC 2 cut(s) 225, 891
BstMBI GATC 3 cut(s) 193, 234, 769
BstMWI GCNNNNNNNGC 2 cut(s) 451, 594
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 2 cut(s) 915, 959
BstSCI CCNGG 3 cut(s) 529, 578, 784
BstSFI CTRYAG 1 cut(s) 810
BstV1I GCAGC 1 cut(s) 454
BstX2I RGATCY 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 1 cut(s) 234
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 2 cut(s) 75, 630
BtgZI GCGATG 1 cut(s) 322
BtsCI GGATG 3 cut(s) 162, 364, 741
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 2 cut(s) 303, 628
CaiI CAGNNNCTG 2 cut(s) 34, 818
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 821
CseI GACGC 1 cut(s) 984
Csp6I GTAC 2 cut(s) 374, 739
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 7 cut(s) 515, 566, 598, 703, 912, 956, 982
CviQI GTAC 2 cut(s) 374, 739
DdeI CTNAG 3 cut(s) 84, 639, 948
DpnI GATC 3 cut(s) 195, 236, 771
DpnII GATC 3 cut(s) 193, 234, 769
DrdI GACNNNNNNGTC 1 cut(s) 617
DseDI GACNNNNNNGTC 1 cut(s) 617
Eam1104I CTCTTC 2 cut(s) 456, 471
EarI CTCTTC 2 cut(s) 456, 471
Eco130I CCWWGG 1 cut(s) 892
Eco147I AGGCCT 1 cut(s) 75
Eco32I GATATC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 821
Eco88I CYCGRG 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 892
ErhI CCWWGG 1 cut(s) 892
Esp3I CGTCTC 1 cut(s) 891
FaeI CATG 7 cut(s) 518, 569, 601, 706, 915, 959, 985
FaqI GGGAC 1 cut(s) 596
FatI CATG 7 cut(s) 514, 565, 597, 702, 911, 955, 981
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 443
FokI GGATG 3 cut(s) 169, 371, 728
Fsp4HI GCNGC 1 cut(s) 443
FspBI CTAG 3 cut(s) 77, 446, 867
GlaI GCGC 1 cut(s) 300
GluI GCNGC 1 cut(s) 443
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 2 cut(s) 75, 630
HapII CCGG 4 cut(s) 290, 579, 785, 969
HgaI GACGC 1 cut(s) 984
HhaI GCGC 1 cut(s) 301
Hin1I GRCGYC 1 cut(s) 995
Hin1II CATG 7 cut(s) 518, 569, 601, 706, 915, 959, 985
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 4 cut(s) 80, 256, 721, 794
HpaII CCGG 4 cut(s) 290, 579, 785, 969
Hpy166II GTNNAC 2 cut(s) 274, 976
Hpy188I TCNGA 9 cut(s) 30, 85, 198, 241, 475, 551, 610, 927, 949
Hpy188III TCNNGA 5 cut(s) 49, 174, 347, 647, 999
Hpy8I GTNNAC 2 cut(s) 274, 976
Hpy99I CGWCG 4 cut(s) 214, 217, 278, 802
HpyAV CCTTC 1 cut(s) 834
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 4 cut(s) 209, 483, 615, 900
HpyF10VI GCNNNNNNNGC 2 cut(s) 451, 594
HpyF3I CTNAG 3 cut(s) 84, 639, 948
HpySE526I ACGT 4 cut(s) 209, 483, 615, 900
Hsp92I GRCGYC 1 cut(s) 995
Hsp92II CATG 7 cut(s) 518, 569, 601, 706, 915, 959, 985
HspAI GCGC 1 cut(s) 299
Kzo9I GATC 3 cut(s) 193, 234, 769
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 3 cut(s) 349, 439, 551
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 2 cut(s) 652, 953
MaeI CTAG 3 cut(s) 77, 446, 867
MaeII ACGT 4 cut(s) 209, 483, 615, 900
MaeIII GTNAC 6 cut(s) 263, 730, 814, 838, 869, 934
MalI GATC 3 cut(s) 195, 236, 771
MboI GATC 3 cut(s) 193, 234, 769
MboII GAAGA 3 cut(s) 473, 488, 773
MfeI CAATTG 1 cut(s) 455
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 5 cut(s) 123, 455, 758, 828, 885
MmeI TCCRAC 2 cut(s) 132, 376
MseI TTAA 3 cut(s) 354, 525, 654
MslI CAYNNNNRTG 2 cut(s) 570, 648
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 4 cut(s) 290, 579, 785, 969
MspR9I CCNGG 3 cut(s) 531, 580, 786
MunI CAATTG 1 cut(s) 455
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 451, 594
NciI CCSGG 2 cut(s) 580, 786
NdeII GATC 3 cut(s) 193, 234, 769
NlaIII CATG 7 cut(s) 518, 569, 601, 706, 915, 959, 985
NlaIV GGNNCC 1 cut(s) 163
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 3 cut(s) 263, 730, 838
NspI RCATGY 2 cut(s) 915, 959
PceI AGGCCT 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 456
PfeI GAWTC 4 cut(s) 80, 256, 721, 794
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 821
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 2 cut(s) 34, 818
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 2 cut(s) 375, 740
RsaNI GTAC 2 cut(s) 374, 739
RseI CAYNNNNRTG 2 cut(s) 570, 648
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 3 cut(s) 354, 525, 654
SatI GCNGC 1 cut(s) 443
Sau3AI GATC 3 cut(s) 193, 234, 769
Sau96I GGNCC 1 cut(s) 821
ScrFI CCNGG 3 cut(s) 531, 580, 786
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 2 cut(s) 652, 953
SfcI CTRYAG 1 cut(s) 810
SinI GGWCC 1 cut(s) 821
SmiMI CAYNNNNRTG 2 cut(s) 570, 648
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SpeI ACTAGT 1 cut(s) 866
Sse9I AATT 5 cut(s) 123, 455, 758, 828, 885
SseBI AGGCCT 1 cut(s) 75
SspI AATATT 1 cut(s) 470
SspMI CTAG 3 cut(s) 77, 446, 867
StuI AGGCCT 1 cut(s) 75
StyD4I CCNGG 3 cut(s) 529, 578, 784
StyI CCWWGG 1 cut(s) 892
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 4 cut(s) 212, 486, 618, 903
TaqI TCGA 4 cut(s) 273, 646, 753, 797
TasI AATT 5 cut(s) 123, 455, 758, 828, 885
TfiI GAWTC 4 cut(s) 80, 256, 721, 794
Tru1I TTAA 3 cut(s) 354, 525, 654
Tru9I TTAA 3 cut(s) 354, 525, 654
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 3 cut(s) 263, 730, 838
TseI GCWGC 1 cut(s) 442
Tsp45I GTSAC 3 cut(s) 263, 730, 838
TspDTI ATGAA 4 cut(s) 325, 573, 734, 771
TspGWI ACGGA 2 cut(s) 815, 888
TspRI CASTG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 821
XapI RAATTY 1 cut(s) 758
XceI RCATGY 2 cut(s) 915, 959
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 3 cut(s) 77, 446, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.