Rh4CG358500

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001016
Physical Location & Seq
Forward (+)
61564165 .. 61567435
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG358500.1

Sequence Viewer

Length: 522 bp
ATGGAGCCGTGGAAGAACACCATGAAGAAGAAGCGGAGGCCCACCCGGAGGCCAGTCCGGCTCCGCTGCTCCGTCAAGAATTACGACTGGGGCATTCTCGGCCACAATTCCAAGGTCGCCAGGCTCTTCGCTCTCAATTCCGGGTCGGACATCGACCCGGCCAAGCCCTACGCCGAGTTCTGGATCGGGACTCATGAATCCGGGCCGTCCTACGCCGAGTCCGGGTCGGAGCAGCCCCTCAGCCTCAAGTCCTGGATCGCTGAGGATCCGAGTGTTCTCGGCGAGAAGGTTCTGGACAAGTGGGGCGCCGATCTTCCGTTCTTGTGCAAGGTACTTTCGGTTCAGAAGGCTCTGTCGATACAGGCTCATCCGGATAAAGAGATGGCTAGAGTGTTGCACAAGTTGCACCCCAGTGTTTACAAGGATGATAATCACAAGCCTGAAGTGGCATTGGCGCTGACGGAGTTCGAGGCTCTTTGCGGGTTTATTAGTCTCAAGGTAAGACAGGGATGGTGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

173

Amino Acids

19.68

Weight (kDa)

9.24

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 20 - 162 2.1e-46 Phosphomannose isomerase type I, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 305
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 3 cut(s) 34, 64, 480
AclWI GGATC 4 cut(s) 191, 260, 263, 273
AcoI YGGCCR 2 cut(s) 100, 159
AcuI CTGAAG 1 cut(s) 462
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 1 cut(s) 333
AfiI CCNNNNNNNGG 3 cut(s) 48, 180, 222
AjnI CCWGG 2 cut(s) 119, 251
AleI CACNNNNGTG 1 cut(s) 411
Alw26I GTCTC 1 cut(s) 497
AlwI GGATC 4 cut(s) 191, 260, 263, 273
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 5 cut(s) 38, 50, 100, 159, 203
ApeKI GCWGC 2 cut(s) 66, 232
AspLEI GCGC 2 cut(s) 308, 457
AspS9I GGNCC 2 cut(s) 39, 203
AsuC2I CCSGG 5 cut(s) 46, 142, 158, 202, 223
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 305
BbvCI CCTCAGC 2 cut(s) 239, 261
BbvI GCAGC 2 cut(s) 53, 244
BccI CCATC 2 cut(s) 376, 504
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 2 cut(s) 121, 253
BcnI CCSGG 5 cut(s) 46, 142, 158, 202, 223
BcoDI GTCTC 1 cut(s) 497
BfaI CTAG 1 cut(s) 387
BfoI RGCGCY 2 cut(s) 309, 458
BglI GCCNNNNNGGC 1 cut(s) 58
BisI GCNGC 2 cut(s) 67, 233
BlsI GCNGC 2 cut(s) 68, 234
Bme1390I CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
BmgT120I GGNCC 2 cut(s) 39, 203
BmiI GGNNCC 4 cut(s) 6, 62, 267, 307
BmrFI CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
BmrI ACTGGG 2 cut(s) 97, 405
BmuI ACTGGG 2 cut(s) 97, 405
Bpu10I CCTNAGC 2 cut(s) 239, 261
BpuEI CTTGAG 2 cut(s) 230, 479
BpuMI CCSGG 5 cut(s) 46, 142, 158, 202, 223
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 8, 111
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 180, 222
Bse1I ACTGG 3 cut(s) 53, 92, 411
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 2 cut(s) 121, 253
BseDI CCNNGG 2 cut(s) 8, 111
BseGI GGATG 3 cut(s) 367, 430, 515
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 48, 180, 222
BseMII CTCAG 2 cut(s) 252, 253
BseNI ACTGG 3 cut(s) 53, 92, 411
BseXI GCAGC 2 cut(s) 53, 244
BshFI GGCC 5 cut(s) 40, 52, 102, 161, 205
BshNI GGYRCC 1 cut(s) 305
BsiSI CCGG 7 cut(s) 46, 58, 141, 158, 201, 222, 371
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 3 cut(s) 48, 180, 222
BsmAI GTCTC 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 5 cut(s) 40, 52, 102, 161, 205
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 4 cut(s) 183, 255, 265, 310
BspACI CCGC 3 cut(s) 34, 64, 480
BspANI GGCC 5 cut(s) 40, 52, 102, 161, 205
BspCNI CTCAG 2 cut(s) 252, 253
BspEI TCCGGA 1 cut(s) 370
BspHI TCATGA 1 cut(s) 193
BspLI GGNNCC 4 cut(s) 6, 62, 267, 307
BspPI GGATC 4 cut(s) 191, 260, 263, 273
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 305
BsrI ACTGG 3 cut(s) 53, 92, 411
BssECI CCNNGG 2 cut(s) 8, 111
BssMI GATC 4 cut(s) 183, 255, 265, 310
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 121, 253
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 403
BstDEI CTNAG 2 cut(s) 239, 261
BstDSI CCRYGG 1 cut(s) 8
BstF5I GGATG 3 cut(s) 367, 430, 515
BstH2I RGCGCY 2 cut(s) 309, 458
BstHHI GCGC 2 cut(s) 308, 457
BstKTI GATC 4 cut(s) 186, 258, 268, 313
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 4 cut(s) 183, 255, 265, 310
BstMWI GCNNNNNNNGC 3 cut(s) 58, 99, 403
BstNI CCWGG 2 cut(s) 121, 253
BstSCI CCNGG 7 cut(s) 44, 119, 140, 156, 200, 221, 251
BstV1I GCAGC 2 cut(s) 53, 244
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 5 cut(s) 40, 52, 102, 161, 205
BtgI CCRYGG 1 cut(s) 8
BtsCI GGATG 3 cut(s) 367, 430, 515
BtsIMutI CAGTG 1 cut(s) 418
CciI TCATGA 1 cut(s) 193
CfoI GCGC 2 cut(s) 308, 457
Cfr13I GGNCC 2 cut(s) 39, 203
Csp6I GTAC 1 cut(s) 332
CviAII CATG 2 cut(s) 22, 194
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 2 cut(s) 239, 261
DinI GGCGCC 1 cut(s) 307
DpnI GATC 4 cut(s) 185, 257, 267, 312
DpnII GATC 4 cut(s) 183, 255, 265, 310
EaeI YGGCCR 2 cut(s) 100, 159
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 119, 251
EcoT14I CCWWGG 1 cut(s) 111
EgeI GGCGCC 1 cut(s) 307
EheI GGCGCC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 25, 197
FaiI YATR 2 cut(s) 23, 195
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 21, 193
FauI CCCGC 1 cut(s) 473
Fnu4HI GCNGC 2 cut(s) 67, 233
FokI GGATG 2 cut(s) 354, 437
Fsp4HI GCNGC 2 cut(s) 67, 233
FspBI CTAG 1 cut(s) 387
GlaI GCGC 2 cut(s) 307, 456
GluI GCNGC 2 cut(s) 67, 233
HaeII RGCGCY 2 cut(s) 309, 458
HaeIII GGCC 5 cut(s) 40, 52, 102, 161, 205
HapII CCGG 7 cut(s) 46, 58, 141, 158, 201, 222, 371
HhaI GCGC 2 cut(s) 308, 457
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 2 cut(s) 25, 197
Hin6I GCGC 2 cut(s) 306, 455
HinP1I GCGC 2 cut(s) 306, 455
HinfI GANTC 3 cut(s) 190, 197, 218
HpaII CCGG 7 cut(s) 46, 58, 141, 158, 201, 222, 371
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 4 cut(s) 148, 229, 270, 345
Hpy188III TCNNGA 6 cut(s) 76, 181, 187, 194, 293, 371
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 2 cut(s) 280, 340
HpyCH4V TGCA 3 cut(s) 327, 397, 406
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 99, 403
HpyF3I CTNAG 2 cut(s) 239, 261
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 2 cut(s) 25, 197
HspAI GCGC 2 cut(s) 306, 455
KasI GGCGCC 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 183, 255, 265, 310
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 4 cut(s) 4, 66, 74, 229
Lsp1109I GCAGC 2 cut(s) 53, 244
MaeI CTAG 1 cut(s) 387
MalI GATC 4 cut(s) 185, 257, 267, 312
MboI GATC 4 cut(s) 183, 255, 265, 310
MboII GAAGA 5 cut(s) 25, 37, 40, 118, 305
MflI RGATCY 1 cut(s) 265
MluCI AATT 3 cut(s) 79, 106, 136
Mly113I GGCGCC 1 cut(s) 306
MlyI GAGTC 2 cut(s) 184, 227
MmeI TCCRAC 2 cut(s) 126, 207
MnlI CCTC 6 cut(s) 30, 42, 248, 254, 256, 463
MroI TCCGGA 1 cut(s) 370
MslI CAYNNNNRTG 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 7 cut(s) 46, 58, 141, 158, 201, 222, 371
MspR9I CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 2 cut(s) 121, 253
MwoI GCNNNNNNNGC 3 cut(s) 58, 99, 403
NarI GGCGCC 1 cut(s) 306
NciI CCSGG 5 cut(s) 46, 142, 158, 202, 223
NdeII GATC 4 cut(s) 183, 255, 265, 310
NlaIII CATG 2 cut(s) 25, 197
NlaIV GGNNCC 4 cut(s) 6, 62, 267, 307
NmeAIII GCCGAG 4 cut(s) 78, 199, 241, 258
OliI CACNNNNGTG 1 cut(s) 411
PagI TCATGA 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 197
PfoI TCCNGGA 1 cut(s) 251
PkrI GCNGC 2 cut(s) 68, 234
PleI GAGTC 2 cut(s) 184, 226
PluTI GGCGCC 1 cut(s) 309
PpsI GAGTC 2 cut(s) 184, 226
Psp6I CCWGG 2 cut(s) 119, 251
PspGI CCWGG 2 cut(s) 119, 251
PspN4I GGNNCC 4 cut(s) 6, 62, 267, 307
PspPI GGNCC 2 cut(s) 39, 203
PsrI GAACNNNNNNTAC 2 cut(s) 324, 356
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 131
SatI GCNGC 2 cut(s) 67, 233
Sau3AI GATC 4 cut(s) 183, 255, 265, 310
Sau96I GGNCC 2 cut(s) 39, 203
SchI GAGTC 2 cut(s) 184, 227
ScrFI CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
SetI ASST 4 cut(s) 117, 291, 333, 501
SfoI GGCGCC 1 cut(s) 307
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 2 cut(s) 245, 494
SmoI CTYRAG 2 cut(s) 245, 494
Sse9I AATT 3 cut(s) 79, 106, 136
SsiI CCGC 3 cut(s) 34, 64, 480
SspDI GGCGCC 1 cut(s) 305
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 7 cut(s) 44, 119, 140, 156, 200, 221, 251
StyI CCWWGG 1 cut(s) 111
TaqI TCGA 3 cut(s) 153, 356, 468
TasI AATT 3 cut(s) 79, 106, 136
TfiI GAWTC 1 cut(s) 197
TscAI CASTG 1 cut(s) 418
TseI GCWGC 2 cut(s) 66, 232
TspDTI ATGAA 2 cut(s) 38, 210
TspGWI ACGGA 3 cut(s) 61, 306, 476
TspRI CASTG 1 cut(s) 418
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.