Rh4BG344100

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001016
Physical Location & Seq
Forward (+)
50662636 .. 50665808
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG344100.1

Sequence Viewer

Length: 1320 bp
ATGGAGCCGTGGAAGAACACCATGAAGAAGAAGCGGAGGCCCACCCGGAGGCCAGTCCGGCTCCGCTGCTCCGTCAAGAATTACGACTGGGGCATTCTCGGCCACAATTCCAAGGTCGCCAGGCTCTTCGCTCTCAATTCCGGGTCGGACATCGACCCGGCCAAGCCCTACGCCGAGTTCTGGATCGGGACTCATGAATCCGGGCCGTCCTACGCCGAGTCCGGGTCGGAGCAGCCCCTCAGCCTCAAGTCCTGGATCGCTGAGGATCCGAGTGTTCTCGGCGAGAAGGTTCTGGACAAGTGGGGCGCCGATCTTCCGTTCTTGTGCAAGGTACTTTCGGTTCAGAAGGCTCTGTCGATACAGGCTCATCCGGATAAAGAGATGGCTAGAGTGTTGCACAAGTTGCACCCCAGTGTTTACAAGGATGATAATCACAAGCCTGAAGTGGCATTGGCGCTGACGGAGTTCGAGGCTCTTTGCGGGTTTATTAGTCTCAAGGAGCTAAAAGACATGCTTCGTGCTGTTCCTGAGATTGCCGAATTGGTTGGGATGGCAGATGCAGAGCAAATTCTACTTGTTGGTGAGCGACATGAGAATGGGAAACAAAACACAGATTTAGAGTCCATGTTCGGTCAACTCATGTTGTCAAGCAAAGAGACAATTTCGAAGATGATATCCAAACTGAAAAGGCGTCTTAACCTTGAGAAAAAGAAAAGGCAATTGACAGTTAAAGAACAACTGGTACTCAGATTAGAGAATCAATATCCAGCCGATGTGGGTGCTATAGCTGCTTTTTTCCTGAACTATGTGAAGCTGAAACGTGGTGAAGCATTATATTGTGGGCCAAATGAACCCCATGCATATATTTCTGGTGAATGCGTTGAATGTATGGCTACCTCTGACAATGTTGTTCGAGCCGGCCTTACCTCAAAACAAATTGATGTTCAGACACTTCTTTCTATGCTCAAATACAGACAGGGAGCTCCTGAAATTATGCAAGGAGTTTCTATGAACGCATACACAACAAGATTTGTCCCACCTTTTGAAGAATTTGAAGTCGACTGCTGTGATCTTCCTCAGTCTGCTTCGGTAGTTTTTCCCTCCGTCATAGGACCATCCGTTTTCCTAGTCACTGCAGGGAAAGGCAGGTTTGATGCAGGCTTGGCTGAGGATGATGTAGTTACAGAAGATGACGATATAGTTGAGGAGGGCGAGGTTCTATTTGTGCCTGCCCACACCAAGATTAGCATTACTGCTAGATCAACAGAACTACAATTGTATAGGGTGGGGGCGAACAGCACATTTTTTAAGGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

439

Amino Acids

49.01

Weight (kDa)

6.07

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 20 - 162 2.6e-45 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 195 - 267 3e-10 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1137
AccB1I GGYRCC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 1059
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 3 cut(s) 34, 64, 480
AclWI GGATC 4 cut(s) 191, 260, 263, 273
AcoI YGGCCR 2 cut(s) 100, 159
AcsI RAATTY 2 cut(s) 567, 1049
AcuI CTGAAG 1 cut(s) 462
AcyI GRCGYC 2 cut(s) 306, 691
AfaI GTAC 2 cut(s) 333, 744
AfiI CCNNNNNNNGG 3 cut(s) 48, 180, 222
AgsI TTSAA 3 cut(s) 884, 1046, 1055
AjnI CCWGG 2 cut(s) 119, 251
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 4 cut(s) 502, 788, 814, 983
AluI AGCT 4 cut(s) 502, 788, 814, 983
Alw21I GWGCWC 1 cut(s) 985
Alw26I GTCTC 2 cut(s) 497, 650
AlwI GGATC 4 cut(s) 191, 260, 263, 273
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 7 cut(s) 38, 50, 100, 159, 203, 842, 919
ApeKI GCWGC 3 cut(s) 66, 232, 788
ApoI RAATTY 2 cut(s) 567, 1049
AspLEI GCGC 2 cut(s) 308, 457
AspS9I GGNCC 4 cut(s) 39, 203, 842, 1112
AsuC2I CCSGG 5 cut(s) 46, 142, 158, 202, 223
AsuHPI GGTGA 3 cut(s) 593, 836, 884
AsuII TTCGAA 1 cut(s) 665
AvaII GGWCC 1 cut(s) 1112
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 985
Bbv12I GWGCWC 1 cut(s) 985
BbvCI CCTCAGC 3 cut(s) 239, 261, 1167
BbvI GCAGC 3 cut(s) 53, 244, 775
BccI CCATC 3 cut(s) 376, 544, 1123
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 2 cut(s) 121, 253
BcnI CCSGG 5 cut(s) 46, 142, 158, 202, 223
BcoDI GTCTC 2 cut(s) 497, 650
BfaI CTAG 3 cut(s) 387, 1127, 1257
BfmI CTRYAG 2 cut(s) 783, 1134
BfoI RGCGCY 2 cut(s) 309, 458
BfuAI ACCTGC 1 cut(s) 1137
BglI GCCNNNNNGGC 1 cut(s) 58
BisI GCNGC 3 cut(s) 67, 233, 789
BlsI GCNGC 3 cut(s) 68, 234, 790
Bme1390I CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
Bme18I GGWCC 1 cut(s) 1112
BmgT120I GGNCC 4 cut(s) 39, 203, 842, 1112
BmiI GGNNCC 4 cut(s) 6, 62, 267, 307
BmrFI CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
BmrI ACTGGG 2 cut(s) 97, 405
BmsI GCATC 2 cut(s) 547, 1144
BmuI ACTGGG 2 cut(s) 97, 405
Bpu10I CCTNAGC 3 cut(s) 239, 261, 1167
Bpu14I TTCGAA 1 cut(s) 665
BpuEI CTTGAG 3 cut(s) 230, 479, 722
BpuMI CCSGG 5 cut(s) 46, 142, 158, 202, 223
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 2 cut(s) 306, 691
BsaJI CCNNGG 2 cut(s) 8, 111
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 180, 222
Bse118I RCCGGY 1 cut(s) 917
Bse1I ACTGG 4 cut(s) 53, 92, 411, 744
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 2 cut(s) 121, 253
BseDI CCNNGG 2 cut(s) 8, 111
BseGI GGATG 5 cut(s) 367, 430, 555, 1115, 1177
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 48, 180, 222
BseMII CTCAG 6 cut(s) 252, 253, 519, 760, 1091, 1158
BseNI ACTGG 4 cut(s) 53, 92, 411, 744
BseRI GAGGAG 1 cut(s) 1220
BseXI GCAGC 3 cut(s) 53, 244, 775
BshFI GGCC 7 cut(s) 40, 52, 102, 161, 205, 844, 921
BshNI GGYRCC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 985
BsiSI CCGG 8 cut(s) 46, 58, 141, 158, 201, 222, 371, 918
BslFI GGGAC 2 cut(s) 202, 1019
BslI CCNNNNNNNGG 3 cut(s) 48, 180, 222
BsmAI GTCTC 2 cut(s) 497, 650
BsmFI GGGAC 2 cut(s) 202, 1019
BsmI GAATGC 2 cut(s) 93, 881
BsnI GGCC 7 cut(s) 40, 52, 102, 161, 205, 844, 921
Bsp119I TTCGAA 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 985
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
BspACI CCGC 3 cut(s) 34, 64, 480
BspANI GGCC 7 cut(s) 40, 52, 102, 161, 205, 844, 921
BspCNI CTCAG 6 cut(s) 252, 253, 520, 759, 1090, 1159
BspEI TCCGGA 1 cut(s) 370
BspHI TCATGA 1 cut(s) 193
BspLI GGNNCC 4 cut(s) 6, 62, 267, 307
BspMAI CTGCAG 1 cut(s) 1138
BspMI ACCTGC 1 cut(s) 1137
BspPI GGATC 4 cut(s) 191, 260, 263, 273
BspQI GCTCTTC 1 cut(s) 131
BspT104I TTCGAA 1 cut(s) 665
BspT107I GGYRCC 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 917
BsrI ACTGG 4 cut(s) 53, 92, 411, 744
BssAI RCCGGY 1 cut(s) 917
BssECI CCNNGG 2 cut(s) 8, 111
BssMI GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
BssNI GRCGYC 2 cut(s) 306, 691
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 121, 253
Bst4CI ACNGT 1 cut(s) 727
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 2 cut(s) 306, 691
BstAPI GCANNNNNTGC 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 919, 1159, 1230
BstDEI CTNAG 6 cut(s) 239, 261, 528, 746, 1077, 1167
BstDSI CCRYGG 1 cut(s) 8
BstF5I GGATG 5 cut(s) 367, 430, 555, 1115, 1177
BstH2I RGCGCY 2 cut(s) 309, 458
BstHHI GCGC 2 cut(s) 308, 457
BstKTI GATC 6 cut(s) 186, 258, 268, 313, 1072, 1262
BstMAI GTCTC 2 cut(s) 497, 650
BstMBI GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
BstMWI GCNNNNNNNGC 5 cut(s) 58, 99, 403, 788, 1163
BstNI CCWGG 2 cut(s) 121, 253
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 7 cut(s) 44, 119, 140, 156, 200, 221, 251
BstSFI CTRYAG 2 cut(s) 783, 1134
BstV1I GCAGC 3 cut(s) 53, 244, 775
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 7 cut(s) 40, 52, 102, 161, 205, 844, 921
BtgI CCRYGG 1 cut(s) 8
BtsCI GGATG 5 cut(s) 367, 430, 555, 1115, 1177
BtsI GCAGTG 1 cut(s) 1131
BtsIMutI CAGTG 2 cut(s) 418, 1131
BveI ACCTGC 1 cut(s) 1137
Cac8I GCNNGC 3 cut(s) 919, 1159, 1230
CciI TCATGA 1 cut(s) 193
CfoI GCGC 2 cut(s) 308, 457
Cfr10I RCCGGY 1 cut(s) 917
Cfr13I GGNCC 4 cut(s) 39, 203, 842, 1112
CseI GACGC 1 cut(s) 680
Csp6I GTAC 2 cut(s) 332, 743
CviAII CATG 7 cut(s) 22, 194, 511, 590, 625, 640, 857
CviQI GTAC 2 cut(s) 332, 743
DdeI CTNAG 6 cut(s) 239, 261, 528, 746, 1077, 1167
DinI GGCGCC 1 cut(s) 307
DpnI GATC 6 cut(s) 185, 257, 267, 312, 1071, 1261
DpnII GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
EaeI YGGCCR 2 cut(s) 100, 159
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 983
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 985
Eco32I GATATC 1 cut(s) 675
Eco47I GGWCC 1 cut(s) 1112
Eco53kI GAGCTC 1 cut(s) 983
Eco57I CTGAAG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 983
EcoRII CCWGG 2 cut(s) 119, 251
EcoRV GATATC 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 862
EcoT38I GRGCYC 1 cut(s) 985
EgeI GGCGCC 1 cut(s) 307
EheI GGCGCC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 7 cut(s) 25, 197, 514, 593, 628, 643, 860
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 2 cut(s) 202, 1019
FatI CATG 7 cut(s) 21, 193, 510, 589, 624, 639, 856
FauI CCCGC 1 cut(s) 473
FblI GTMKAC 1 cut(s) 1059
Fnu4HI GCNGC 3 cut(s) 67, 233, 789
FokI GGATG 5 cut(s) 354, 437, 562, 1102, 1184
FriOI GRGCYC 1 cut(s) 985
Fsp4HI GCNGC 3 cut(s) 67, 233, 789
FspBI CTAG 3 cut(s) 387, 1127, 1257
GlaI GCGC 2 cut(s) 307, 456
GluI GCNGC 3 cut(s) 67, 233, 789
HaeII RGCGCY 2 cut(s) 309, 458
HaeIII GGCC 7 cut(s) 40, 52, 102, 161, 205, 844, 921
HapII CCGG 8 cut(s) 46, 58, 141, 158, 201, 222, 371, 918
HgaI GACGC 1 cut(s) 680
HhaI GCGC 2 cut(s) 308, 457
Hin1I GRCGYC 2 cut(s) 306, 691
Hin1II CATG 7 cut(s) 25, 197, 514, 593, 628, 643, 860
Hin6I GCGC 2 cut(s) 306, 455
HinP1I GCGC 2 cut(s) 306, 455
HincII GTYRAC 2 cut(s) 635, 1060
HindII GTYRAC 2 cut(s) 635, 1060
HinfI GANTC 5 cut(s) 190, 197, 218, 620, 757
HpaII CCGG 8 cut(s) 46, 58, 141, 158, 201, 222, 371, 918
HphI GGTGA 3 cut(s) 593, 836, 884
Hpy166II GTNNAC 3 cut(s) 418, 635, 1060
Hpy188I TCNGA 7 cut(s) 148, 229, 270, 345, 749, 901, 948
Hpy188III TCNNGA 9 cut(s) 76, 181, 187, 194, 293, 371, 527, 799, 986
Hpy8I GTNNAC 3 cut(s) 418, 635, 1060
HpyAV CCTTC 2 cut(s) 280, 340
HpyCH4III ACNGT 1 cut(s) 727
HpyCH4IV ACGT 1 cut(s) 820
HpyCH4V TGCA 8 cut(s) 327, 397, 406, 560, 860, 997, 1136, 1157
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 99, 403, 788, 1163
HpyF3I CTNAG 6 cut(s) 239, 261, 528, 746, 1077, 1167
HpySE526I ACGT 1 cut(s) 820
Hsp92I GRCGYC 2 cut(s) 306, 691
Hsp92II CATG 7 cut(s) 25, 197, 514, 593, 628, 643, 860
HspAI GCGC 2 cut(s) 306, 455
KasI GGCGCC 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 370
KroI GCCGGC 1 cut(s) 917
KroNI GCCGGC 1 cut(s) 919
Kzo9I GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 7 cut(s) 4, 66, 74, 229, 499, 980, 988
Lsp1109I GCAGC 3 cut(s) 53, 244, 775
LweI GCATC 2 cut(s) 547, 1144
MaeI CTAG 3 cut(s) 387, 1127, 1257
MaeII ACGT 1 cut(s) 820
MaeIII GTNAC 2 cut(s) 1129, 1180
MalI GATC 6 cut(s) 185, 257, 267, 312, 1071, 1261
MboI GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
MboII GAAGA 9 cut(s) 25, 37, 40, 118, 305, 679, 1058, 1064, 1199
MfeI CAATTG 2 cut(s) 719, 1274
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 985
Mly113I GGCGCC 1 cut(s) 306
MlyI GAGTC 3 cut(s) 184, 227, 629
MmeI TCCRAC 2 cut(s) 126, 207
Mph1103I ATGCAT 1 cut(s) 862
MroI TCCGGA 1 cut(s) 370
MroNI GCCGGC 1 cut(s) 917
MseI TTAA 3 cut(s) 696, 729, 1308
MslI CAYNNNNRTG 2 cut(s) 411, 594
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 8 cut(s) 46, 58, 141, 158, 201, 222, 371, 918
MspR9I CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
MunI CAATTG 2 cut(s) 719, 1274
Mva1269I GAATGC 2 cut(s) 93, 881
MvaI CCWGG 2 cut(s) 121, 253
MwoI GCNNNNNNNGC 5 cut(s) 58, 99, 403, 788, 1163
NaeI GCCGGC 1 cut(s) 919
NarI GGCGCC 1 cut(s) 306
NciI CCSGG 5 cut(s) 46, 142, 158, 202, 223
NdeII GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
NgoMIV GCCGGC 1 cut(s) 917
NlaIII CATG 7 cut(s) 25, 197, 514, 593, 628, 643, 860
NlaIV GGNNCC 4 cut(s) 6, 62, 267, 307
NmeAIII GCCGAG 4 cut(s) 78, 199, 241, 258
NmuCI GTSAC 1 cut(s) 1129
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 1 cut(s) 514
NspV TTCGAA 1 cut(s) 665
OliI CACNNNNGTG 1 cut(s) 411
PagI TCATGA 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 2 cut(s) 93, 881
PdiI GCCGGC 1 cut(s) 919
PfeI GAWTC 2 cut(s) 197, 757
PfoI TCCNGGA 1 cut(s) 251
PkrI GCNGC 3 cut(s) 68, 234, 790
PleI GAGTC 3 cut(s) 184, 226, 628
PluTI GGCGCC 1 cut(s) 309
PpsI GAGTC 3 cut(s) 184, 226, 628
Psp124BI GAGCTC 1 cut(s) 985
Psp6I CCWGG 2 cut(s) 119, 251
PspGI CCWGG 2 cut(s) 119, 251
PspN4I GGNNCC 4 cut(s) 6, 62, 267, 307
PspPI GGNCC 4 cut(s) 39, 203, 842, 1112
PsrI GAACNNNNNNTAC 4 cut(s) 324, 356, 726, 758
PstI CTGCAG 1 cut(s) 1138
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 2 cut(s) 333, 744
RsaNI GTAC 2 cut(s) 332, 743
RseI CAYNNNNRTG 2 cut(s) 411, 594
SacI GAGCTC 1 cut(s) 985
SalI GTCGAC 1 cut(s) 1058
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 3 cut(s) 696, 729, 1308
SatI GCNGC 3 cut(s) 67, 233, 789
Sau3AI GATC 6 cut(s) 183, 255, 265, 310, 1069, 1259
Sau96I GGNCC 4 cut(s) 39, 203, 842, 1112
SchI GAGTC 3 cut(s) 184, 227, 629
ScrFI CCNGG 7 cut(s) 46, 121, 142, 158, 202, 223, 253
SduI GDGCHC 1 cut(s) 985
SfaNI GCATC 2 cut(s) 547, 1144
SfcI CTRYAG 2 cut(s) 783, 1134
SfoI GGCGCC 1 cut(s) 307
SfuI TTCGAA 1 cut(s) 665
SinI GGWCC 1 cut(s) 1112
SmiMI CAYNNNNRTG 2 cut(s) 411, 594
SmlI CTYRAG 3 cut(s) 245, 494, 701
SmoI CTYRAG 3 cut(s) 245, 494, 701
SsiI CCGC 3 cut(s) 34, 64, 480
SspDI GGCGCC 1 cut(s) 305
SspMI CTAG 3 cut(s) 387, 1127, 1257
SstI GAGCTC 1 cut(s) 985
StyD4I CCNGG 7 cut(s) 44, 119, 140, 156, 200, 221, 251
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 727
TaiI ACGT 1 cut(s) 823
TaqI TCGA 6 cut(s) 153, 356, 468, 665, 913, 1059
TaqII GACCGA 1 cut(s) 620
TfiI GAWTC 2 cut(s) 197, 757
Tru1I TTAA 3 cut(s) 696, 729, 1308
Tru9I TTAA 3 cut(s) 696, 729, 1308
TscAI CASTG 2 cut(s) 418, 1138
TseFI GTSAC 1 cut(s) 1129
TseI GCWGC 3 cut(s) 66, 232, 788
Tsp45I GTSAC 1 cut(s) 1129
TspDTI ATGAA 4 cut(s) 38, 210, 864, 1025
TspGWI ACGGA 5 cut(s) 61, 306, 476, 1093, 1108
TspRI CASTG 2 cut(s) 418, 1138
VpaK11BI GGWCC 1 cut(s) 1112
XapI RAATTY 2 cut(s) 567, 1049
XceI RCATGY 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 1059
XspI CTAG 3 cut(s) 387, 1127, 1257
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.