Rorug04G0279800

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001016
Physical Location & Seq
Forward (+)
45251149 .. 45252389
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0279800.1

Sequence Viewer

Length: 1170 bp
ATGGCCTCCATAGACGCTGTTATAGCAAGTTTTGCTGTTTCTCTTGCCCTCGCTACGGGCGGTAGCGCCCCGGATCTGGGCAAGATTGGTGGTGGTCTGGTGCGCAAGTCATCTCAATCTTTTCTACTTGGCAAACCTCTGACTCGCAAGCCAGTGGATCCCAAAGCGTTCAAGGATTTCCATCGTACTTGGATGGTGGATAAGGATTTTAGGGCTCAGGAAAAGGCTGGAGATGTGTTCCTATTCTCCTTTGAATCTTTCCGTGATTGTAACAATATTCTTCGAGGAGGGGTTTGGTCCTTTGATCGAGCTCCAGTTTGTTTTGAAGAATATGATGGCGTAAAGCCTCTGCGTGAAACCCACTTCAAGCATCTTCGTGTGTGGGTTAGTGTTTTAGGGATTCCTCCTTTGTATGAAGAACCTGAGAATTTTTCCCTTATTGGTAATCTCTTGGGTGGTTTCTTAGACTATGATAAGAAGGAATTCAAGAAGGGTGTGATTCAGATCTTCTTTACCCATGACATCTCCAAACCGTTCTTACTTGAGCAAAAAGTTTTTTTGGATGCCGATGTCGAACCTGTGCTACAATTTCAATTTGAGCATCTGAAAGGGAGATGCTCACAGTGTGGACTAGTCACGCATGCTGGGATTCCATGTGAGGAGCCGAACACTACAATTTCGACGCCTAGGGTTTTGAGTTTTCCGGGACCATCAACCTCAAGAGGCTCGTTTTCTTTCTCGACTCAGAACAAGCGTGAGATTTTTACTGCTTCCTCCTCTATCCCCAAGAAGAAGAAGCCAACTATTCATCGAGTTGAGCGTCCTATTCCCCCATGTACTTCATCTGCGGGGTCGCCTAATGGTTCGGACCCGGATGGGGAGAATACTGTTTCGTCAGTGGAACAAGGCATGATGCAGGTTGTGCAACTGGATGTGGTGGTTTCTAAGAAAAGGGATAGGAGTGGTAGTTTCAATCCCTCTCCAAAGAAGTTCAAGACCATTTTGGGTGGTAAGATCCCAACCTTGCAGGCTGATGCTTTGGGACTGATTGAAGATGACAATGATACTCCTCTTGTCACCCTAAAGAAGAAGATGGATAGGCCACTTGGCAGCAACAATAAGAGCCAACGCTCTGGTAAGAAGTCGGGTGCTGCCCCTTTGAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

389

Amino Acids

42.79

Weight (kDa)

9.29

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 174 - 219 1.1e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 907
AciI CCGC 2 cut(s) 60, 848
AclWI GGATC 4 cut(s) 81, 152, 165, 1009
AcsI RAATTY 2 cut(s) 427, 482
AcyI GRCGYC 1 cut(s) 683
AdeI CACNNNGTG 1 cut(s) 626
AfaI GTAC 2 cut(s) 187, 838
AfiI CCNNNNNNNGG 5 cut(s) 55, 76, 77, 877, 1161
AgsI TTSAA 9 cut(s) 172, 254, 326, 367, 487, 593, 973, 994, 1052
AhlI ACTAGT 1 cut(s) 631
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Alw21I GWGCWC 1 cut(s) 313
AlwI GGATC 4 cut(s) 81, 152, 165, 1009
AoxI GGCC 2 cut(s) 3, 1100
ApeKI GCWGC 2 cut(s) 1110, 1151
ApoI RAATTY 2 cut(s) 427, 482
Asp700I GAANNNNTTC 1 cut(s) 482
AspA2I CCTAGG 1 cut(s) 686
AspLEI GCGC 2 cut(s) 68, 105
AspS9I GGNCC 3 cut(s) 297, 707, 868
AsuC2I CCSGG 3 cut(s) 71, 705, 872
AsuHPI GGTGA 1 cut(s) 1069
AvaII GGWCC 3 cut(s) 297, 707, 868
AvrII CCTAGG 1 cut(s) 686
BamHI GGATCC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 217, 313
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 2 cut(s) 1122, 1138
BccI CCATC 6 cut(s) 187, 189, 329, 718, 869, 1087
BcnI CCSGG 3 cut(s) 71, 705, 872
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 2 cut(s) 632, 687
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 907
BglII AGATCT 1 cut(s) 504
BisI GCNGC 2 cut(s) 1111, 1152
BlnI CCTAGG 1 cut(s) 686
BlsI GCNGC 2 cut(s) 1112, 1153
Bme1390I CCNGG 3 cut(s) 71, 705, 872
Bme18I GGWCC 3 cut(s) 297, 707, 868
BmgT120I GGNCC 3 cut(s) 297, 707, 868
BmiI GGNNCC 4 cut(s) 159, 663, 708, 870
BmrFI CCNGG 3 cut(s) 71, 705, 872
BmsI GCATC 6 cut(s) 379, 553, 605, 610, 903, 1024
BpmI CTGGAG 2 cut(s) 249, 297
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 2 cut(s) 563, 703
BpuMI CCSGG 3 cut(s) 71, 705, 872
BsaBI GATNNNNATC 2 cut(s) 180, 503
BsaHI GRCGYC 1 cut(s) 683
BsaJI CCNNGG 2 cut(s) 69, 686
BsaXI ACNNNNNCTCC 2 cut(s) 952, 982
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 76, 77, 877, 1161
Bse1I ACTGG 3 cut(s) 152, 314, 933
Bse8I GATNNNNATC 2 cut(s) 180, 503
BseDI CCNNGG 2 cut(s) 69, 686
BseGI GGATG 4 cut(s) 198, 568, 880, 937
BseJI GATNNNNATC 2 cut(s) 180, 503
BseLI CCNNNNNNNGG 5 cut(s) 55, 76, 77, 877, 1161
BseMII CTCAG 3 cut(s) 230, 414, 758
BseNI ACTGG 3 cut(s) 152, 314, 933
BseRI GAGGAG 4 cut(s) 300, 674, 766, 1059
BseXI GCAGC 2 cut(s) 1122, 1138
BseYI CCCAGC 1 cut(s) 644
BshFI GGCC 2 cut(s) 5, 1102
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 3 cut(s) 71, 704, 872
BslFI GGGAC 2 cut(s) 720, 1056
BslI CCNNNNNNNGG 5 cut(s) 55, 76, 77, 877, 1161
BsmFI GGGAC 2 cut(s) 720, 1056
BsnI GGCC 2 cut(s) 5, 1102
Bsp1286I GDGCHC 2 cut(s) 217, 313
Bsp143I GATC 5 cut(s) 73, 157, 304, 504, 1014
BspACI CCGC 2 cut(s) 60, 848
BspANI GGCC 2 cut(s) 5, 1102
BspCNI CTCAG 3 cut(s) 229, 415, 757
BspLI GGNNCC 4 cut(s) 159, 663, 708, 870
BspMI ACCTGC 1 cut(s) 907
BspPI GGATC 4 cut(s) 81, 152, 165, 1009
BsrI ACTGG 3 cut(s) 152, 314, 933
BssECI CCNNGG 2 cut(s) 69, 686
BssMI GATC 5 cut(s) 73, 157, 304, 504, 1014
BssNI GRCGYC 1 cut(s) 683
BssT1I CCWWGG 1 cut(s) 686
Bst4CI ACNGT 3 cut(s) 534, 624, 889
BstACI GRCGYC 1 cut(s) 683
BstAPI GCANNNNNTGC 2 cut(s) 32, 922
BstC8I GCNNGC 3 cut(s) 149, 642, 1029
BstDEI CTNAG 5 cut(s) 216, 423, 463, 744, 945
BstF5I GGATG 4 cut(s) 198, 568, 880, 937
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 105
BstKTI GATC 5 cut(s) 76, 160, 307, 507, 1017
BstMBI GATC 5 cut(s) 73, 157, 304, 504, 1014
BstMWI GCNNNNNNNGC 3 cut(s) 23, 32, 922
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 3 cut(s) 69, 703, 870
BstV1I GCAGC 2 cut(s) 1122, 1138
BstX2I RGATCY 4 cut(s) 73, 157, 504, 1014
BstXI CCANNNNNNTGG 1 cut(s) 1133
BstYI RGATCY 4 cut(s) 73, 157, 504, 1014
BsuRI GGCC 2 cut(s) 5, 1102
BtsCI GGATG 4 cut(s) 198, 568, 880, 937
BtsIMutI CAGTG 3 cut(s) 159, 629, 903
BveI ACCTGC 1 cut(s) 907
Cac8I GCNNGC 3 cut(s) 149, 642, 1029
CfoI GCGC 2 cut(s) 68, 105
Cfr13I GGNCC 3 cut(s) 297, 707, 868
CseI GACGC 3 cut(s) 23, 691, 809
Csp6I GTAC 2 cut(s) 186, 837
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
CviAII CATG 5 cut(s) 518, 641, 654, 834, 910
CviQI GTAC 2 cut(s) 186, 837
DdeI CTNAG 5 cut(s) 216, 423, 463, 744, 945
DpnI GATC 5 cut(s) 75, 159, 306, 506, 1016
DpnII GATC 5 cut(s) 73, 157, 304, 504, 1014
DraIII CACNNNGTG 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 686
Eco24I GRGCYC 2 cut(s) 217, 313
Eco47I GGWCC 3 cut(s) 297, 707, 868
Eco53kI GAGCTC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 482
EcoT14I CCWWGG 1 cut(s) 686
EcoT38I GRGCYC 2 cut(s) 217, 313
ErhI CCWWGG 1 cut(s) 686
FaeI CATG 5 cut(s) 521, 644, 657, 837, 913
FaqI GGGAC 2 cut(s) 720, 1056
FatI CATG 5 cut(s) 517, 640, 653, 833, 909
FauI CCCGC 1 cut(s) 841
Fnu4HI GCNGC 2 cut(s) 1111, 1152
FokI GGATG 4 cut(s) 205, 575, 887, 944
FriOI GRGCYC 2 cut(s) 217, 313
Fsp4HI GCNGC 2 cut(s) 1111, 1152
FspBI CTAG 2 cut(s) 632, 687
FspI TGCGCA 1 cut(s) 104
GlaI GCGC 2 cut(s) 67, 104
GluI GCNGC 2 cut(s) 1111, 1152
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 2 cut(s) 249, 297
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 2 cut(s) 5, 1102
HapII CCGG 3 cut(s) 71, 704, 872
HgaI GACGC 3 cut(s) 23, 691, 809
HhaI GCGC 2 cut(s) 68, 105
Hin1I GRCGYC 1 cut(s) 683
Hin1II CATG 5 cut(s) 521, 644, 657, 837, 913
Hin6I GCGC 2 cut(s) 66, 103
HinP1I GCGC 2 cut(s) 66, 103
HinfI GANTC 6 cut(s) 142, 254, 400, 499, 649, 742
HpaII CCGG 3 cut(s) 71, 704, 872
HphI GGTGA 1 cut(s) 1069
Hpy166II GTNNAC 1 cut(s) 629
Hpy188I TCNGA 5 cut(s) 141, 504, 606, 747, 868
Hpy188III TCNNGA 5 cut(s) 218, 487, 720, 739, 994
Hpy8I GTNNAC 1 cut(s) 629
Hpy99I CGWCG 1 cut(s) 685
HpyAV CCTTC 2 cut(s) 472, 484
HpyCH4III ACNGT 3 cut(s) 534, 624, 889
HpyCH4V TGCA 3 cut(s) 916, 925, 1027
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 32, 922
HpyF3I CTNAG 5 cut(s) 216, 423, 463, 744, 945
Hsp92I GRCGYC 1 cut(s) 683
Hsp92II CATG 5 cut(s) 521, 644, 657, 837, 913
HspAI GCGC 2 cut(s) 66, 103
Kzo9I GATC 5 cut(s) 73, 157, 304, 504, 1014
LmnI GCTCC 2 cut(s) 316, 661
Lsp1109I GCAGC 2 cut(s) 1122, 1138
LweI GCATC 6 cut(s) 379, 553, 605, 610, 903, 1024
MaeI CTAG 2 cut(s) 632, 687
MaeIII GTNAC 3 cut(s) 269, 634, 1075
MalI GATC 5 cut(s) 75, 159, 306, 506, 1016
MboI GATC 5 cut(s) 73, 157, 304, 504, 1014
MflI RGATCY 4 cut(s) 73, 157, 504, 1014
MhlI GDGCHC 2 cut(s) 217, 313
MluCI AATT 5 cut(s) 427, 482, 587, 593, 675
MlyI GAGTC 2 cut(s) 136, 736
MroXI GAANNNNTTC 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 375
MspI CCGG 3 cut(s) 71, 704, 872
MspR9I CCNGG 3 cut(s) 71, 705, 872
MwoI GCNNNNNNNGC 3 cut(s) 23, 32, 922
NciI CCSGG 3 cut(s) 71, 705, 872
NdeII GATC 5 cut(s) 73, 157, 304, 504, 1014
NlaIII CATG 5 cut(s) 521, 644, 657, 837, 913
NlaIV GGNNCC 4 cut(s) 159, 663, 708, 870
NmuCI GTSAC 2 cut(s) 634, 1075
NsbI TGCGCA 1 cut(s) 104
NspI RCATGY 1 cut(s) 644
PaeI GCATGC 1 cut(s) 644
PcsI WCGNNNNNNNCGW 1 cut(s) 817
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 4 cut(s) 254, 400, 499, 649
PfoI TCCNGGA 1 cut(s) 703
PkrI GCNGC 2 cut(s) 1112, 1153
PleI GAGTC 2 cut(s) 136, 736
PpsI GAGTC 2 cut(s) 136, 736
Psp124BI GAGCTC 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 644
PspN4I GGNNCC 4 cut(s) 159, 663, 708, 870
PspPI GGNCC 3 cut(s) 297, 707, 868
PsrI GAACNNNNNNTAC 2 cut(s) 567, 599
PsuI RGATCY 4 cut(s) 73, 157, 504, 1014
RsaI GTAC 2 cut(s) 187, 838
RsaNI GTAC 2 cut(s) 186, 837
RseI CAYNNNNRTG 1 cut(s) 375
SacI GAGCTC 1 cut(s) 313
SatI GCNGC 2 cut(s) 1111, 1152
Sau3AI GATC 5 cut(s) 73, 157, 304, 504, 1014
Sau96I GGNCC 3 cut(s) 297, 707, 868
SchI GAGTC 2 cut(s) 136, 736
ScrFI CCNGG 3 cut(s) 71, 705, 872
SduI GDGCHC 2 cut(s) 217, 313
SetI ASST 7 cut(s) 139, 313, 424, 580, 719, 921, 1025
SfaNI GCATC 6 cut(s) 379, 553, 605, 610, 903, 1024
SinI GGWCC 3 cut(s) 297, 707, 868
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 2 cut(s) 542, 718
SmoI CTYRAG 2 cut(s) 542, 718
SpeI ACTAGT 1 cut(s) 631
SphI GCATGC 1 cut(s) 644
Sse9I AATT 5 cut(s) 427, 482, 587, 593, 675
SsiI CCGC 2 cut(s) 60, 848
SspI AATATT 1 cut(s) 277
SspMI CTAG 2 cut(s) 632, 687
SstI GAGCTC 1 cut(s) 313
StyD4I CCNGG 3 cut(s) 69, 703, 870
StyI CCWWGG 1 cut(s) 686
TaaI ACNGT 3 cut(s) 534, 624, 889
TaqI TCGA 6 cut(s) 283, 307, 573, 680, 740, 811
TasI AATT 5 cut(s) 427, 482, 587, 593, 675
TatI WGTACW 1 cut(s) 836
TfiI GAWTC 4 cut(s) 254, 400, 499, 649
TscAI CASTG 3 cut(s) 159, 629, 903
TseFI GTSAC 2 cut(s) 634, 1075
TseI GCWGC 2 cut(s) 1110, 1151
Tsp45I GTSAC 2 cut(s) 634, 1075
TspDTI ATGAA 3 cut(s) 429, 797, 831
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 3 cut(s) 159, 629, 903
VpaK11BI GGWCC 3 cut(s) 297, 707, 868
XapI RAATTY 2 cut(s) 427, 482
XceI RCATGY 1 cut(s) 644
XmaJI CCTAGG 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 2 cut(s) 632, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.