MD17G1244800.v1.1

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001016
Physical Location & Seq
Reverse (-)
29320749 .. 29323928
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1244800.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGGACTCCGTCATCAAGAAATCAATGAAGCATCAAAACCAGAGGACTGGTTCGCTTCAGCGGCTGAGATGCTCCGTTCAGAACTACGATTGGGGGAAGAGGGGACAAGATTCCCAAGTGGCCAGGCTCTCTGCTTTCAACTCTGGTTCTGGGATCAACCCAGAAAAATCATATGCGGAATTTTGGATGGGAACCCATGAGTCCGGACCTTCCTTTTTGATTCAGAATTCGGATGAGAATAATGGGTTTTTCCCAATTGGGTCTCAGATTCTATCTCTCAAAGATTGGATTTCCAAAAACCCAAATGTGCTTGGTGAGAAGGTGATCCAAAAATGGGGCTCTGACCTCCCTTTTTTGTTCAAGGTGCTTTCTGTGGGGAAGGCATTGTCGATACAGGCACACCCAGATAAGGATTTGGCCAAGATTTTGCACAAGTTGAAGCCAAATGTTTACAAGGATGCTAATCACAAGCCTGAGATGGCCCTAGCCACAACCCACTTTGAGGCTCTGTGTGGTTTCATCACTCCTCAGGAGCTTAAAGTTGTGCTTGCTAATGTCCCTGAGATTGAAGAACTAATTGGCAGTGAAGAAGCAAAAAAAGTTTCCGGAATCACTGATCAAGACAAGGAGGAGAAAGTAACGTCCGCTCTGCGTTCGGTTTTCACCCACCTCATGTTAGCCAGCAAGGAGATGATAACCACAGTTATTACTAAAATGAAGGATCGTTTACACATCGAAAGTCAGGCGAGGCGTTTGACAGAGAAGGAACAGCTGGTATTGCAGTTGGTAAAGCAATATCCAACTGATGTGGGTGTTATATCTGCCTTCTTCATGAACCATGTGAAACTTAATCCCGGAGAAGCGCTGTACATCGGGGCTAATGAACCCCACGCCTACATATTTGGTGAGTGCGTTGAGTGCATGGCAACCTCAGACAACGTAGTGAGGGCTGGCCTTACTCCAAAGCATATGGATGTTCAAACCCTTTGTTCTATGCTCACATACAAGCAGGGGTATCCTGACATTCTGCAAGGAGTTGCAGTAGGTCCTTATGTGACAAGGTATCTCCCGCCTTTCGATGAGTTTGAGGTTGATCGCTGCCAACTTCCGCAGGGAGAATCAGCAGAATTCCCTGCAGCCCCAGGGCCTTCGATTTTTGTGGTCACGTTCGGGGAGGGAATTATATACGCAAGCGATGTTCAAGGGGATGTAATTACAGAAGGAGACGTTCTTTTTGCACCAGCAAACACTCGGATTCGCATAACAGGCGCATCCGAGTTGCAGATATATCGAGCGGGTGTGAGCAGCCTGTTCTTCCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

442

Amino Acids

48.9

Weight (kDa)

6.04

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 19 - 173 1.7e-45 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 192 - 277 1.7e-12 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 647, 1295
AccIII TCCGGA 2 cut(s) 203, 605
AciI CCGC 6 cut(s) 61, 176, 645, 1070, 1109, 1295
AclWI GGATC 3 cut(s) 161, 319, 729
AcoI YGGCCR 2 cut(s) 120, 417
AcsI RAATTY 3 cut(s) 179, 226, 1127
AcuI CTGAAG 1 cut(s) 41
AfaI GTAC 1 cut(s) 869
AfeI AGCGCT 1 cut(s) 864
AfiI CCNNNNNNNGG 3 cut(s) 334, 409, 502
AgsI TTSAA 6 cut(s) 139, 361, 439, 569, 980, 1202
AjnI CCWGG 2 cut(s) 122, 1141
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 3 cut(s) 535, 772, 1322
AluI AGCT 3 cut(s) 535, 772, 1322
Alw26I GTCTC 2 cut(s) 267, 1218
AlwI GGATC 3 cut(s) 161, 319, 729
AlwNI CAGNNNCTG 1 cut(s) 64
Aor13HI TCCGGA 2 cut(s) 203, 605
Aor51HI AGCGCT 1 cut(s) 864
AoxI GGCC 5 cut(s) 120, 417, 480, 952, 1145
ApeKI GCWGC 3 cut(s) 1098, 1136, 1305
ApoI RAATTY 3 cut(s) 179, 226, 1127
ArsI GACNNNNNNTTYG 2 cut(s) 1217, 1249
AspLEI GCGC 2 cut(s) 865, 1271
AspS9I GGNCC 4 cut(s) 206, 481, 1046, 1145
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 4 cut(s) 326, 334, 655, 917
AvaII GGWCC 2 cut(s) 206, 1046
AxyI CCTNAGG 1 cut(s) 528
BalI TGGCCA 2 cut(s) 122, 419
BanII GRGCYC 1 cut(s) 341
BbvI GCAGC 3 cut(s) 1085, 1148, 1317
BccI CCATC 2 cut(s) 181, 472
BcgI CGANNNNNNTGC 2 cut(s) 1271, 1305
BciT130I CCWGG 2 cut(s) 124, 1143
BciVI GTATCC 1 cut(s) 1026
BclI TGATCA 1 cut(s) 616
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 2 cut(s) 267, 1218
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 1 cut(s) 1134
BfoI RGCGCY 1 cut(s) 866
BfuI GTATCC 1 cut(s) 1026
BisI GCNGC 4 cut(s) 62, 1099, 1137, 1306
BlsI GCNGC 4 cut(s) 63, 1100, 1138, 1307
Bme1390I CCNGG 3 cut(s) 124, 855, 1143
Bme18I GGWCC 2 cut(s) 206, 1046
BmgT120I GGNCC 4 cut(s) 206, 481, 1046, 1145
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 3 cut(s) 124, 855, 1143
BmsI GCATC 4 cut(s) 40, 59, 448, 1280
BpuMI CCSGG 1 cut(s) 855
BsaBI GATNNNNATC 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 1141, 1142
BsaWI WCCGGW 2 cut(s) 203, 605
BsaXI ACNNNNNCTCC 2 cut(s) 1026, 1056
Bsc4I CCNNNNNNNGG 3 cut(s) 334, 409, 502
Bse1I ACTGG 1 cut(s) 52
Bse21I CCTNAGG 1 cut(s) 528
Bse8I GATNNNNATC 1 cut(s) 462
BseAI TCCGGA 2 cut(s) 203, 605
BseBI CCWGG 2 cut(s) 124, 1143
BseDI CCNNGG 2 cut(s) 1141, 1142
BseGI GGATG 6 cut(s) 192, 238, 463, 979, 1213, 1271
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 3 cut(s) 334, 409, 502
BseMII CTCAG 6 cut(s) 56, 278, 465, 542, 552, 945
BseNI ACTGG 1 cut(s) 52
BseRI GAGGAG 2 cut(s) 516, 644
BseXI GCAGC 3 cut(s) 1085, 1148, 1317
BshFI GGCC 5 cut(s) 122, 419, 482, 954, 1147
BsiSI CCGG 3 cut(s) 204, 606, 855
BslFI GGGAC 2 cut(s) 117, 542
BslI CCNNNNNNNGG 3 cut(s) 334, 409, 502
BsmAI GTCTC 2 cut(s) 267, 1218
BsmBI CGTCTC 1 cut(s) 1218
BsmFI GGGAC 2 cut(s) 117, 542
BsnI GGCC 5 cut(s) 122, 419, 482, 954, 1147
Bso31I GGTCTC 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 341
Bsp13I TCCGGA 2 cut(s) 203, 605
Bsp1407I TGTACA 1 cut(s) 867
Bsp143I GATC 5 cut(s) 153, 324, 616, 721, 1093
BspACI CCGC 6 cut(s) 61, 176, 645, 1070, 1109, 1295
BspANI GGCC 5 cut(s) 122, 419, 482, 954, 1147
BspCNI CTCAG 6 cut(s) 57, 277, 466, 541, 553, 944
BspEI TCCGGA 2 cut(s) 203, 605
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 1 cut(s) 193
BspMAI CTGCAG 1 cut(s) 1138
BspPI GGATC 3 cut(s) 161, 319, 729
BspTNI GGTCTC 1 cut(s) 267
BsrBI CCGCTC 2 cut(s) 647, 1295
BsrGI TGTACA 1 cut(s) 867
BsrI ACTGG 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 1141, 1142
BssMI GATC 5 cut(s) 153, 324, 616, 721, 1093
Bst2UI CCWGG 2 cut(s) 124, 1143
Bst4CI ACNGT 1 cut(s) 703
Bst6I CTCTTC 1 cut(s) 92
BstAUI TGTACA 1 cut(s) 867
BstC8I GCNNGC 4 cut(s) 549, 682, 952, 1192
BstDEI CTNAG 6 cut(s) 65, 264, 474, 528, 561, 931
BstF5I GGATG 6 cut(s) 192, 238, 463, 979, 1213, 1271
BstH2I RGCGCY 1 cut(s) 866
BstHHI GCGC 2 cut(s) 865, 1271
BstKTI GATC 5 cut(s) 156, 327, 619, 724, 1096
BstMAI GTCTC 2 cut(s) 267, 1218
BstMBI GATC 5 cut(s) 153, 324, 616, 721, 1093
BstMWI GCNNNNNNNGC 4 cut(s) 61, 778, 918, 1266
BstNI CCWGG 2 cut(s) 124, 1143
BstSCI CCNGG 3 cut(s) 122, 853, 1141
BstSFI CTRYAG 1 cut(s) 1134
BstV1I GCAGC 3 cut(s) 1085, 1148, 1317
BstXI CCANNNNNNTGG 1 cut(s) 47
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 1026
BsuRI GGCC 5 cut(s) 122, 419, 482, 954, 1147
BtgZI GCGATG 1 cut(s) 1209
BtsCI GGATG 6 cut(s) 192, 238, 463, 979, 1213, 1271
BtsI GCAGTG 1 cut(s) 589
BtsIMutI CAGTG 2 cut(s) 589, 612
Cac8I GCNNGC 4 cut(s) 549, 682, 952, 1192
CaiI CAGNNNCTG 1 cut(s) 64
CciI TCATGA 1 cut(s) 831
CfoI GCGC 2 cut(s) 865, 1271
Cfr13I GGNCC 4 cut(s) 206, 481, 1046, 1145
Csp6I GTAC 1 cut(s) 868
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 5 cut(s) 197, 673, 832, 839, 922
CviQI GTAC 1 cut(s) 868
DdeI CTNAG 6 cut(s) 65, 264, 474, 528, 561, 931
DpnI GATC 5 cut(s) 155, 326, 618, 723, 1095
DpnII GATC 5 cut(s) 153, 324, 616, 721, 1093
EaeI YGGCCR 2 cut(s) 120, 417
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 267
Eco47I GGWCC 2 cut(s) 206, 1046
Eco47III AGCGCT 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 41
Eco81I CCTNAGG 1 cut(s) 528
EcoO109I RGGNCCY 2 cut(s) 1046, 1145
EcoRI GAATTC 2 cut(s) 226, 1127
EcoRII CCWGG 2 cut(s) 122, 1141
EcoT38I GRGCYC 1 cut(s) 341
Esp3I CGTCTC 1 cut(s) 1218
FaeI CATG 5 cut(s) 200, 676, 835, 842, 925
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FaqI GGGAC 2 cut(s) 117, 542
FatI CATG 5 cut(s) 196, 672, 831, 838, 921
FauI CCCGC 2 cut(s) 1077, 1288
FauNDI CATATG 2 cut(s) 172, 969
FbaI TGATCA 1 cut(s) 616
Fnu4HI GCNGC 4 cut(s) 62, 1099, 1137, 1306
FokI GGATG 6 cut(s) 199, 245, 470, 986, 1220, 1258
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 4 cut(s) 62, 1099, 1137, 1306
FspBI CTAG 1 cut(s) 485
GlaI GCGC 2 cut(s) 864, 1270
GluI GCNGC 4 cut(s) 62, 1099, 1137, 1306
HaeII RGCGCY 1 cut(s) 866
HaeIII GGCC 5 cut(s) 122, 419, 482, 954, 1147
HapII CCGG 3 cut(s) 204, 606, 855
HhaI GCGC 2 cut(s) 865, 1271
Hin1II CATG 5 cut(s) 200, 676, 835, 842, 925
Hin6I GCGC 2 cut(s) 863, 1269
HinP1I GCGC 2 cut(s) 863, 1269
HindIII AAGCTT 1 cut(s) 1320
HinfI GANTC 8 cut(s) 5, 110, 200, 220, 268, 609, 1118, 1255
HpaII CCGG 3 cut(s) 204, 606, 855
HphI GGTGA 4 cut(s) 326, 334, 655, 917
Hpy166II GTNNAC 2 cut(s) 451, 728
Hpy188I TCNGA 8 cut(s) 81, 225, 232, 267, 343, 934, 1254, 1276
Hpy188III TCNNGA 7 cut(s) 16, 204, 530, 606, 620, 832, 1019
Hpy8I GTNNAC 2 cut(s) 451, 728
HpyAV CCTTC 8 cut(s) 219, 313, 373, 712, 757, 835, 1158, 1214
HpyCH4III ACNGT 1 cut(s) 703
HpyCH4IV ACGT 4 cut(s) 641, 939, 1166, 1227
HpyCH4V TGCA 8 cut(s) 430, 781, 921, 1030, 1040, 1136, 1238, 1282
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 778, 918, 1266
HpyF3I CTNAG 6 cut(s) 65, 264, 474, 528, 561, 931
HpySE526I ACGT 4 cut(s) 641, 939, 1166, 1227
Hsp92II CATG 5 cut(s) 200, 676, 835, 842, 925
HspAI GCGC 2 cut(s) 863, 1269
Kpn2I TCCGGA 2 cut(s) 203, 605
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 5 cut(s) 153, 324, 616, 721, 1093
LmnI GCTCC 2 cut(s) 77, 532
Lsp1109I GCAGC 3 cut(s) 1085, 1148, 1317
LweI GCATC 4 cut(s) 40, 59, 448, 1280
MaeI CTAG 1 cut(s) 485
MaeII ACGT 4 cut(s) 641, 939, 1166, 1227
MaeIII GTNAC 3 cut(s) 637, 1054, 1162
MalI GATC 5 cut(s) 155, 326, 618, 723, 1095
MbiI CCGCTC 2 cut(s) 647, 1295
MboI GATC 5 cut(s) 153, 324, 616, 721, 1093
MboII GAAGA 5 cut(s) 109, 581, 599, 820, 1306
MfeI CAATTG 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 341
MlsI TGGCCA 2 cut(s) 122, 419
MluCI AATT 7 cut(s) 179, 226, 255, 576, 1127, 1179, 1212
MluNI TGGCCA 2 cut(s) 122, 419
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 824
Mox20I TGGCCA 2 cut(s) 122, 419
MroI TCCGGA 2 cut(s) 203, 605
MscI TGGCCA 2 cut(s) 122, 419
MseI TTAA 2 cut(s) 537, 849
MslI CAYNNNNRTG 1 cut(s) 972
Msp20I TGGCCA 2 cut(s) 122, 419
MspA1I CMGCKG 2 cut(s) 61, 772
MspI CCGG 3 cut(s) 204, 606, 855
MspR9I CCNGG 3 cut(s) 124, 855, 1143
MunI CAATTG 1 cut(s) 255
MvaI CCWGG 2 cut(s) 124, 1143
MwoI GCNNNNNNNGC 4 cut(s) 61, 778, 918, 1266
NciI CCSGG 1 cut(s) 855
NdeI CATATG 2 cut(s) 172, 969
NdeII GATC 5 cut(s) 153, 324, 616, 721, 1093
NlaIII CATG 5 cut(s) 200, 676, 835, 842, 925
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 2 cut(s) 1054, 1162
PagI TCATGA 1 cut(s) 831
PasI CCCWGGG 1 cut(s) 1142
PfeI GAWTC 6 cut(s) 110, 220, 268, 609, 1118, 1255
PflFI GACNNNGTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 853
PkrI GCNGC 4 cut(s) 63, 1100, 1138, 1307
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PpuMI RGGWCCY 1 cut(s) 1046
Psp5II RGGWCCY 1 cut(s) 1046
Psp6I CCWGG 2 cut(s) 122, 1141
PspGI CCWGG 2 cut(s) 122, 1141
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 4 cut(s) 206, 481, 1046, 1145
PspPPI RGGWCCY 1 cut(s) 1046
PstI CTGCAG 1 cut(s) 1138
PstNI CAGNNNCTG 1 cut(s) 64
PsyI GACNNNGTC 1 cut(s) 8
PvuII CAGCTG 1 cut(s) 772
RsaI GTAC 1 cut(s) 869
RsaNI GTAC 1 cut(s) 868
RseI CAYNNNNRTG 1 cut(s) 972
SaqAI TTAA 2 cut(s) 537, 849
SatI GCNGC 4 cut(s) 62, 1099, 1137, 1306
Sau3AI GATC 5 cut(s) 153, 324, 616, 721, 1093
Sau96I GGNCC 4 cut(s) 206, 481, 1046, 1145
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 3 cut(s) 124, 855, 1143
SduI GDGCHC 1 cut(s) 341
SfaNI GCATC 4 cut(s) 40, 59, 448, 1280
SfcI CTRYAG 1 cut(s) 1134
SinI GGWCC 2 cut(s) 206, 1046
SmiMI CAYNNNNRTG 1 cut(s) 972
Sse9I AATT 7 cut(s) 179, 226, 255, 576, 1127, 1179, 1212
SsiI CCGC 6 cut(s) 61, 176, 645, 1070, 1109, 1295
SspMI CTAG 1 cut(s) 485
StyD4I CCNGG 3 cut(s) 122, 853, 1141
TaaI ACNGT 1 cut(s) 703
TaiI ACGT 4 cut(s) 644, 942, 1169, 1230
TaqI TCGA 5 cut(s) 389, 735, 1077, 1151, 1291
TasI AATT 7 cut(s) 179, 226, 255, 576, 1127, 1179, 1212
TatI WGTACW 1 cut(s) 867
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 6 cut(s) 110, 220, 268, 609, 1118, 1255
Tru1I TTAA 2 cut(s) 537, 849
Tru9I TTAA 2 cut(s) 537, 849
TscAI CASTG 2 cut(s) 589, 619
TseFI GTSAC 2 cut(s) 1054, 1162
TseI GCWGC 3 cut(s) 1098, 1136, 1305
Tsp45I GTSAC 2 cut(s) 1054, 1162
TspDTI ATGAA 6 cut(s) 41, 508, 731, 820, 848, 897
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 2 cut(s) 589, 619
Tth111I GACNNNGTC 1 cut(s) 8
VpaK11BI GGWCC 2 cut(s) 206, 1046
XapI RAATTY 3 cut(s) 179, 226, 1127
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.