Rh5AG527200

Belongs to the mannose-6-phosphate isomerase type 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001016
Physical Location & Seq
Forward (+)
90423895 .. 90430376
6482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG527200.1

Sequence Viewer

Length: 1281 bp
ATGGAGAAGCAGATTCTACAGAGGCTGAGATGCTCGGTCCAGAATTACGACTGGGGTAAGAAAGGCGGAGACTCTGAGGTGGGCAGGCTCTATGCCTACAACTCTGGTTCTGAAATTGACCCAGAAATGCCTTACGCAGAGTTTTGGATGGGAACCCATGACTCTGGACCATCCTTTTTGATTGATAAGGATCTGAATATTAACACGAGGAATGAATCGTTTTTGCTTCTCAAAGACTGGGTTTCTGATAAGCCTCATGACGTGCTGGGTCACAAGGTCGTCGAGAAGTGGGGCTCTGACCTGCCTTTCTTATTTAAGGTGCTTTCGGTGGCAAAGGCTTTGTCAATCCAGGCACATCCAGATCAGGACTTGGCCAAGGCTTTGCACAAGCTGATGCCAAACATTTACAAGGACGACAATCACAAGCCTGAGATGGCGCTTGCTCTAACACATTTCCAGGCTCTTTGTGGTTTTATTACTGTTGAGGAGCTAAAGGGAGTGCTTCATAGTGTCCCAGAGATTGCACAAGTGGTGGGTAGTGAAGTCGCAAGCCAAGTGATTCACAGTACTGCTGAAGATGGGGAGGATAAAGTCAAGTCTGTTCTACGTTCAATTTTCACCCAACTTATGTTAGCTAATAAAGAGGTGATAAGTACAGTAACAACCACATTGAAAAGTCGTCTACTTATGGAAAGTCAGACGAGGCAGTTGACAGAGAAGGAAGAGCTGGTGTTGCAATTGGAAAAGCAATATCCAGATGATGTGGGCGTTATATCTGCCTTCTTTCTCAACCATGTCAACCTTAATCCTGGAGAAGCACTATACCTTGGAGCAAATGAACCCCATGCCTATTTATCCGGCGACTGCATCGAGTGCATGGCAAGCTCGGACAACGTAGTGAGGGCCGGCCTTACTCCCAAGCATCGGGATATTAAAACCCTTTGTTCTATGCTCACATACAAACAGGGCTTTCCTGAAATCCTACAGGGAGATTCTATAAATCCTTATGTAACAAGGTACATCCCACCTTTTGAAGAATTTGAGGTTGATCGATGTCATCTTCCTGAAGGAAAATCCATAGAATTTTCTGCAAGCCCAGGTCCTTCCCTTTTTGTGGTAACTTTTGGAGTGGGAGCAATATATACAGGTGGTACAAAATATGGTATCACCCGAGGAGACGTTCTTTTTGCACCCGCTGACACTGAGATGAGCATAAGAAGTGAATCTGAGTTGCAGATATATAGAGCCGGAGTGAACAGCAAGTTCCTTCGAGCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000032 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004476 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006056 GO:0006057 GO:0006082 GO:0006139 GO:0006464 GO:0006486 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006807 GO:0007275 GO:0007584 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009108 GO:0009110 GO:0009225 GO:0009226 GO:0009298 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010154 GO:0014070 GO:0016043 GO:0016051 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0017085 GO:0018130 GO:0019438 GO:0019538 GO:0019673 GO:0019752 GO:0019852 GO:0019853 GO:0022414 GO:0031505 GO:0031506 GO:0031667 GO:0032025 GO:0032501 GO:0032502 GO:0033273 GO:0033591 GO:0034284 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042221 GO:0042364 GO:0042493 GO:0042546 GO:0043170 GO:0043412 GO:0043413 GO:0043436 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045229 GO:0046364 GO:0046394 GO:0046483 GO:0046680 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0070085 GO:0070589 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0071852 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

426

Amino Acids

47.45

Weight (kDa)

5.37

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 6 - 158 3.4e-47 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 178 - 263 2.1e-15 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 309
AccI GTMKAC 1 cut(s) 682
AciI CCGC 2 cut(s) 66, 1194
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 2 cut(s) 1037, 1082
AcuI CTGAAG 2 cut(s) 594, 1086
AfaI GTAC 4 cut(s) 568, 655, 1019, 1153
AfiI CCNNNNNNNGG 2 cut(s) 924, 1114
AgsI TTSAA 3 cut(s) 612, 673, 1034
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 4 cut(s) 348, 456, 808, 1096
AluBI AGCT 5 cut(s) 391, 490, 635, 727, 885
AluI AGCT 5 cut(s) 391, 490, 635, 727, 885
Alw26I GTCTC 2 cut(s) 63, 1170
AlwI GGATC 1 cut(s) 198
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 1170
AoxI GGCC 3 cut(s) 372, 903, 907
ApoI RAATTY 2 cut(s) 1037, 1082
ArsI GACNNNNNNTTYG 4 cut(s) 326, 358, 1169, 1201
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 4 cut(s) 37, 167, 903, 1100
AsuHPI GGTGA 3 cut(s) 610, 658, 1159
AvaI CYCGRG 1 cut(s) 1170
AvaII GGWCC 3 cut(s) 37, 167, 1100
BalI TGGCCA 1 cut(s) 374
BanII GRGCYC 1 cut(s) 296
BauI CACGAG 1 cut(s) 205
BccI CCATC 4 cut(s) 142, 178, 427, 572
BciT130I CCWGG 4 cut(s) 350, 458, 810, 1098
BcoDI GTCTC 2 cut(s) 63, 1170
BfmI CTRYAG 2 cut(s) 17, 983
BfoI RGCGCY 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 309
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 4 cut(s) 350, 458, 810, 1098
Bme18I GGWCC 3 cut(s) 37, 167, 1100
BmeT110I CYCGRG 1 cut(s) 1170
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 4 cut(s) 37, 167, 903, 1100
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 4 cut(s) 350, 458, 810, 1098
BmrI ACTGGG 2 cut(s) 61, 247
BmsI GCATC 4 cut(s) 20, 384, 876, 931
BmuI ACTGGG 2 cut(s) 61, 247
BpmI CTGGAG 1 cut(s) 831
Bsa29I ATCGAT 1 cut(s) 1051
BsaBI GATNNNNATC 1 cut(s) 189
BsaJI CCNNGG 4 cut(s) 375, 826, 1096, 1171
Bsc4I CCNNNNNNNGG 2 cut(s) 924, 1114
Bse118I RCCGGY 1 cut(s) 905
Bse1I ACTGG 2 cut(s) 56, 242
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 4 cut(s) 350, 458, 810, 1098
BseCI ATCGAT 1 cut(s) 1051
BseDI CCNNGG 4 cut(s) 375, 826, 1096, 1171
BseGI GGATG 4 cut(s) 153, 170, 355, 1020
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 924, 1114
BseMII CTCAG 5 cut(s) 17, 66, 420, 1194, 1218
BseNI ACTGG 2 cut(s) 56, 242
BseRI GAGGAG 2 cut(s) 500, 1188
BseYI CCCAGC 1 cut(s) 265
BshFI GGCC 3 cut(s) 374, 905, 909
BshVI ATCGAT 1 cut(s) 1051
BsiHKCI CYCGRG 1 cut(s) 1170
BsiSI CCGG 3 cut(s) 858, 906, 1248
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 2 cut(s) 924, 1114
BsmAI GTCTC 2 cut(s) 63, 1170
BsmBI CGTCTC 1 cut(s) 1170
BsmFI GGGAC 1 cut(s) 497
BsnI GGCC 3 cut(s) 374, 905, 909
BsoBI CYCGRG 1 cut(s) 1170
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 3 cut(s) 190, 361, 1048
BspACI CCGC 2 cut(s) 66, 1194
BspANI GGCC 3 cut(s) 374, 905, 909
BspCNI CTCAG 5 cut(s) 18, 67, 421, 1195, 1219
BspDI ATCGAT 1 cut(s) 1051
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 309
BspPI GGATC 1 cut(s) 198
BspQI GCTCTTC 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 905
BsrI ACTGG 2 cut(s) 56, 242
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 4 cut(s) 375, 826, 1096, 1171
BssMI GATC 3 cut(s) 190, 361, 1048
BssSI CACGAG 1 cut(s) 205
BssT1I CCWWGG 2 cut(s) 375, 826
Bst2BI CACGAG 1 cut(s) 205
Bst2UI CCWGG 4 cut(s) 350, 458, 810, 1098
Bst4CI ACNGT 3 cut(s) 481, 566, 658
Bst6I CTCTTC 1 cut(s) 717
BstAPI GCANNNNNTGC 1 cut(s) 873
BstC8I GCNNGC 6 cut(s) 86, 441, 550, 883, 907, 1093
BstDEI CTNAG 5 cut(s) 26, 75, 429, 1203, 1227
BstF5I GGATG 4 cut(s) 153, 170, 355, 1020
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 3 cut(s) 193, 364, 1051
BstMAI GTCTC 2 cut(s) 63, 1170
BstMBI GATC 3 cut(s) 190, 361, 1048
BstMWI GCNNNNNNNGC 3 cut(s) 733, 873, 882
BstNI CCWGG 4 cut(s) 350, 458, 810, 1098
BstSCI CCNGG 4 cut(s) 348, 456, 808, 1096
BstSFI CTRYAG 2 cut(s) 17, 983
BstX2I RGATCY 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 164
BstYI RGATCY 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 1051
BsuRI GGCC 3 cut(s) 374, 905, 909
BsuTUI ATCGAT 1 cut(s) 1051
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 153, 170, 355, 1020
BtsIMutI CAGTG 1 cut(s) 1200
BveI ACCTGC 1 cut(s) 309
Cac8I GCNNGC 6 cut(s) 86, 441, 550, 883, 907, 1093
CaiI CAGNNNCTG 1 cut(s) 25
CciI TCATGA 1 cut(s) 256
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 37, 167, 903, 1100
ClaI ATCGAT 1 cut(s) 1051
Csp6I GTAC 4 cut(s) 567, 654, 1018, 1152
CviAII CATG 5 cut(s) 158, 257, 794, 845, 877
CviQI GTAC 4 cut(s) 567, 654, 1018, 1152
DdeI CTNAG 5 cut(s) 26, 75, 429, 1203, 1227
DpnI GATC 3 cut(s) 192, 363, 1050
DpnII GATC 3 cut(s) 190, 361, 1048
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 375, 826
Eco24I GRGCYC 1 cut(s) 296
Eco47I GGWCC 3 cut(s) 37, 167, 1100
Eco57I CTGAAG 2 cut(s) 594, 1086
Eco88I CYCGRG 1 cut(s) 1170
EcoO109I RGGNCCY 1 cut(s) 1100
EcoRII CCWGG 4 cut(s) 348, 456, 808, 1096
EcoT14I CCWWGG 2 cut(s) 375, 826
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 2 cut(s) 375, 826
Esp3I CGTCTC 1 cut(s) 1170
FaeI CATG 5 cut(s) 161, 260, 797, 848, 880
FaqI GGGAC 1 cut(s) 497
FatI CATG 5 cut(s) 157, 256, 793, 844, 876
FauI CCCGC 1 cut(s) 1201
FblI GTMKAC 1 cut(s) 682
FokI GGATG 4 cut(s) 157, 160, 342, 1007
FriOI GRGCYC 1 cut(s) 296
FseI GGCCGGCC 1 cut(s) 909
GlaI GCGC 1 cut(s) 438
GsaI CCCAGC 1 cut(s) 269
GsuI CTGGAG 1 cut(s) 831
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 3 cut(s) 374, 905, 909
HapII CCGG 3 cut(s) 858, 906, 1248
HhaI GCGC 1 cut(s) 439
Hin1II CATG 5 cut(s) 161, 260, 797, 848, 880
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HincII GTYRAC 2 cut(s) 711, 799
HindII GTYRAC 2 cut(s) 711, 799
HinfI GANTC 7 cut(s) 13, 71, 161, 215, 559, 992, 1223
HpaII CCGG 3 cut(s) 858, 906, 1248
HphI GGTGA 3 cut(s) 610, 658, 1159
Hpy166II GTNNAC 4 cut(s) 683, 711, 799, 1255
Hpy188I TCNGA 8 cut(s) 76, 112, 195, 247, 298, 699, 889, 1228
Hpy8I GTNNAC 4 cut(s) 683, 711, 799, 1255
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 5 cut(s) 712, 790, 1061, 1113, 1277
HpyCH4III ACNGT 3 cut(s) 481, 566, 658
HpyCH4IV ACGT 4 cut(s) 261, 607, 894, 1179
HpyCH4V TGCA 8 cut(s) 385, 524, 736, 867, 876, 1091, 1190, 1234
HpyF10VI GCNNNNNNNGC 3 cut(s) 733, 873, 882
HpyF3I CTNAG 5 cut(s) 26, 75, 429, 1203, 1227
HpySE526I ACGT 4 cut(s) 261, 607, 894, 1179
Hsp92II CATG 5 cut(s) 161, 260, 797, 848, 880
HspAI GCGC 1 cut(s) 437
KroI GCCGGC 1 cut(s) 905
KroNI GCCGGC 1 cut(s) 907
Kzo9I GATC 3 cut(s) 190, 361, 1048
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 3 cut(s) 487, 830, 1133
LweI GCATC 4 cut(s) 20, 384, 876, 931
MaeII ACGT 4 cut(s) 261, 607, 894, 1179
MaeIII GTNAC 4 cut(s) 269, 658, 1009, 1117
MalI GATC 3 cut(s) 192, 363, 1050
MboI GATC 3 cut(s) 190, 361, 1048
MboII GAAGA 4 cut(s) 587, 734, 1046, 1052
MfeI CAATTG 1 cut(s) 737
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 296
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 6 cut(s) 43, 114, 612, 737, 1037, 1082
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 2 cut(s) 65, 155
Mox20I TGGCCA 1 cut(s) 374
MroNI GCCGGC 1 cut(s) 905
MscI TGGCCA 1 cut(s) 374
MseI TTAA 4 cut(s) 201, 315, 804, 933
MslI CAYNNNNRTG 1 cut(s) 1205
Msp20I TGGCCA 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 1196
MspI CCGG 3 cut(s) 858, 906, 1248
MspR9I CCNGG 4 cut(s) 350, 458, 810, 1098
MunI CAATTG 1 cut(s) 737
MvaI CCWGG 4 cut(s) 350, 458, 810, 1098
MwoI GCNNNNNNNGC 3 cut(s) 733, 873, 882
NaeI GCCGGC 1 cut(s) 907
NdeII GATC 3 cut(s) 190, 361, 1048
NgoMIV GCCGGC 1 cut(s) 905
NlaIII CATG 5 cut(s) 161, 260, 797, 848, 880
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 269
PagI TCATGA 1 cut(s) 256
PciSI GCTCTTC 1 cut(s) 717
PdiI GCCGGC 1 cut(s) 907
PfeI GAWTC 5 cut(s) 13, 215, 559, 992, 1223
PfoI TCCNGGA 1 cut(s) 808
PleI GAGTC 2 cut(s) 65, 155
PpsI GAGTC 2 cut(s) 65, 155
PpuMI RGGWCCY 1 cut(s) 1100
Psp5II RGGWCCY 1 cut(s) 1100
Psp6I CCWGG 4 cut(s) 348, 456, 808, 1096
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 4 cut(s) 348, 456, 808, 1096
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 4 cut(s) 37, 167, 903, 1100
PspPPI RGGWCCY 1 cut(s) 1100
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 190
RigI GGCCGGCC 1 cut(s) 909
RsaI GTAC 4 cut(s) 568, 655, 1019, 1153
RsaNI GTAC 4 cut(s) 567, 654, 1018, 1152
RseI CAYNNNNRTG 1 cut(s) 1205
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 4 cut(s) 201, 315, 804, 933
Sau3AI GATC 3 cut(s) 190, 361, 1048
Sau96I GGNCC 4 cut(s) 37, 167, 903, 1100
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 2 cut(s) 65, 155
ScrFI CCNGG 4 cut(s) 350, 458, 810, 1098
SduI GDGCHC 1 cut(s) 296
SfaNI GCATC 4 cut(s) 20, 384, 876, 931
SfcI CTRYAG 2 cut(s) 17, 983
SinI GGWCC 3 cut(s) 37, 167, 1100
SmiMI CAYNNNNRTG 1 cut(s) 1205
Sse9I AATT 6 cut(s) 43, 114, 612, 737, 1037, 1082
SsiI CCGC 2 cut(s) 66, 1194
SspI AATATT 1 cut(s) 199
StyD4I CCNGG 4 cut(s) 348, 456, 808, 1096
StyI CCWWGG 2 cut(s) 375, 826
TaaI ACNGT 3 cut(s) 481, 566, 658
TaiI ACGT 4 cut(s) 264, 610, 897, 1182
TaqI TCGA 4 cut(s) 282, 870, 1051, 1270
TaqII GACCGA 1 cut(s) 25
TasI AATT 6 cut(s) 43, 114, 612, 737, 1037, 1082
TatI WGTACW 2 cut(s) 566, 653
TfiI GAWTC 5 cut(s) 13, 215, 559, 992, 1223
Tru1I TTAA 4 cut(s) 201, 315, 804, 933
Tru9I TTAA 4 cut(s) 201, 315, 804, 933
TscAI CASTG 1 cut(s) 1207
TseFI GTSAC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 3 cut(s) 228, 494, 852
TspRI CASTG 1 cut(s) 1207
VpaK11BI GGWCC 3 cut(s) 37, 167, 1100
XapI RAATTY 2 cut(s) 1037, 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 464
XmiI GTMKAC 1 cut(s) 682
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.