MD13G1039700.v1.1

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001016
Physical Location & Seq
Forward (+)
2708485 .. 2711186
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1039700.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGAGCCGCCGTGGGACAAGAGCATGAAGAAGAAGCGGAAGCGGAGCCGCCGCCCCGTCAGGCTGAGATGCTCCGTCAAGAATTACGATTGGGGCATTGTCGGCCGCAATTCCAAGGTCGCCAGGCTTTTCGCGCTCAATTCCGGGTCTGACATCGACCCGGGCAAGCCGTACGCGGAGTTCTGGATCGGAACTCATGAATCTGGGCCGTCGTATGCGGATTCCGGGTCGGAGCCCTTGAGTCTCAAGGCCTGGATCTCGCAGGATCCTAGTGTGCTTGGAGACAAGGTGGTTGAAAAATGGGGTGCTGATCTTCCGTTTTTGTTTAAGGTGCTTTCGGTTGGGAAGGCGTTGTCGATACAGGCTCATCCGGATAAAGAACTGGCTAGGGTGTTGCACAAGTTACACCCCAGTGTTTTCCGAGATGACAATCACAAGCCTGAAATGGCATTGGCATTGACGGAATTCGAGGCTCTTTGTGGGTTTATCAGTGTCAAGGAGCTTAAGGACATGCTTAATAGTGTTCCCGAGATTGCAGAATTGGTTGTGGTTGGAGATGCAGAGCGAATTGTACTTGGTAGTGAGCACCATGAGAATGGGATAGCCAAAACAGATATAGAATCCGTCTTCACTCGACTTATGTTACTAAGTAAAGATACAATTGCTGAGATGATTTCTAGACTGAAAAGACGCCTTAATCTAGAGAAAAAGAAAAGGCATTTGACGGTTAAAGAACAACTGGTACTCAGATTGGAGAGTCAATATCCAGATGATGTGGGTGCAATAGCAGCGTTTTTTCTTAACTATGTGAAGCTGAACCGTGGTGAAGCAATAAGTCTTGGGCCAAATGAACCTCATGCATATATTTCTGGTGAATGCATTGAGTGTATGGCAACTTCTGACAATGTTGTTCGTGCTGGCCTTACCTCAAAACCATTGGATGTCCAAACACTTCTTTCTATGCTCGAATATAGACAGGGTTCTCCTGAAATTATGCAAGGCGTTTCTTTGAATCCATACACAACAAGGTATCTCCCACCTTTTGAAGAATTTGAAGTTGACTGCTGTAACCTTCCTCACTCTGCATCGGTGGTTTTTCCTTCAGTCCTAGGACCATCCCTCTTCCTGTTTACTGCCGGGAAAGGACATTTCGATGCAAGTCTGTCTGAGGATGATATAGTGATGGAGGGTGATGATATAGTTGAAGAGGGTGAGGTTTTCTTTGTGCCTGCCAACACGAAGATTAGCATTACCGCCAAATCAACAGAACTACAATTGTACAGGGTGGGAGTGAACAGCAGAATATTTAGGGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

440

Amino Acids

49.02

Weight (kDa)

5.95

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 21 - 162 6e-45 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 204 - 267 5.6e-09 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 134, 176
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 9 cut(s) 8, 37, 43, 49, 52, 106, 176, 218, 1254
AclWI GGATC 4 cut(s) 194, 260, 263, 273
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 464, 1049
AcuI CTGAAG 1 cut(s) 1086
AcyI GRCGYC 1 cut(s) 691
AfaI GTAC 4 cut(s) 173, 573, 744, 1280
AflII CTTAAG 1 cut(s) 503
AgsI TTSAA 5 cut(s) 296, 1012, 1046, 1055, 1205
AjnI CCWGG 2 cut(s) 122, 251
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 2 cut(s) 502, 814
AluI AGCT 2 cut(s) 502, 814
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 2 cut(s) 248, 276
AlwI GGATC 4 cut(s) 194, 260, 263, 273
Ama87I CYCGRG 2 cut(s) 160, 527
Aor13HI TCCGGA 1 cut(s) 370
AoxI GGCC 5 cut(s) 103, 206, 249, 842, 919
ApeKI GCWGC 1 cut(s) 788
ApoI RAATTY 2 cut(s) 464, 1049
AspA2I CCTAGG 1 cut(s) 1108
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 3 cut(s) 206, 842, 1112
AsuC2I CCSGG 5 cut(s) 145, 161, 162, 226, 1138
AsuHPI GGTGA 4 cut(s) 836, 884, 1202, 1223
AvaI CYCGRG 2 cut(s) 160, 527
AvaII GGWCC 1 cut(s) 1112
AvrII CCTAGG 1 cut(s) 1108
BamHI GGATCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 880, 912
BbsI GAAGAC 1 cut(s) 619
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 800
BccI CCATC 2 cut(s) 1123, 1177
BceAI ACGGC 2 cut(s) 154, 193
BciT130I CCWGG 2 cut(s) 124, 253
BcnI CCSGG 5 cut(s) 145, 161, 162, 226, 1138
BcoDI GTCTC 2 cut(s) 248, 276
BfaI CTAG 5 cut(s) 270, 387, 678, 701, 1109
BfrI CTTAAG 1 cut(s) 503
BisI GCNGC 5 cut(s) 8, 49, 52, 106, 789
BlnI CCTAGG 1 cut(s) 1108
BlsI GCNGC 5 cut(s) 9, 50, 53, 107, 790
Bme1390I CCNGG 7 cut(s) 124, 145, 161, 162, 226, 253, 1138
Bme18I GGWCC 1 cut(s) 1112
BmeT110I CYCGRG 2 cut(s) 160, 527
BmgT120I GGNCC 3 cut(s) 206, 842, 1112
BmiI GGNNCC 4 cut(s) 6, 47, 234, 267
BmrFI CCNGG 7 cut(s) 124, 145, 161, 162, 226, 253, 1138
BmrI ACTGGG 1 cut(s) 405
BmsI GCATC 4 cut(s) 59, 547, 1094, 1144
BmuI ACTGGG 1 cut(s) 405
BpiI GAAGAC 1 cut(s) 619
BpuEI CTTGAG 2 cut(s) 230, 259
BpuMI CCSGG 5 cut(s) 145, 161, 162, 226, 1138
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 691
BsaJI CCNNGG 5 cut(s) 11, 114, 160, 820, 1108
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 3 cut(s) 26, 273, 303
Bse1I ACTGG 3 cut(s) 387, 411, 744
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 2 cut(s) 124, 253
BseDI CCNNGG 5 cut(s) 11, 114, 160, 820, 1108
BseGI GGATG 4 cut(s) 367, 946, 1115, 1177
BseJI GATNNNNATC 1 cut(s) 429
BseMII CTCAG 4 cut(s) 56, 657, 760, 1158
BseNI ACTGG 3 cut(s) 387, 411, 744
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 1 cut(s) 800
Bsh1236I CGCG 2 cut(s) 134, 176
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 5 cut(s) 105, 208, 251, 844, 921
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 2 cut(s) 160, 527
BsiSI CCGG 5 cut(s) 144, 161, 225, 371, 1137
BsiWI CGTACG 1 cut(s) 171
BslFI GGGAC 1 cut(s) 29
BsmAI GTCTC 2 cut(s) 248, 276
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 881
BsnI GGCC 5 cut(s) 105, 208, 251, 844, 921
BsoBI CYCGRG 2 cut(s) 160, 527
Bsp1286I GDGCHC 2 cut(s) 237, 588
Bsp13I TCCGGA 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 1278
Bsp143I GATC 4 cut(s) 186, 255, 265, 310
BspACI CCGC 9 cut(s) 8, 37, 43, 49, 52, 106, 176, 218, 1254
BspANI GGCC 5 cut(s) 105, 208, 251, 844, 921
BspCNI CTCAG 4 cut(s) 57, 658, 759, 1159
BspEI TCCGGA 1 cut(s) 370
BspFNI CGCG 2 cut(s) 134, 176
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 4 cut(s) 6, 47, 234, 267
BspPI GGATC 4 cut(s) 194, 260, 263, 273
BspTI CTTAAG 1 cut(s) 503
BsrGI TGTACA 1 cut(s) 1278
BsrI ACTGG 3 cut(s) 387, 411, 744
BssECI CCNNGG 5 cut(s) 11, 114, 160, 820, 1108
BssMI GATC 4 cut(s) 186, 255, 265, 310
BssNI GRCGYC 1 cut(s) 691
BssT1I CCWWGG 2 cut(s) 114, 1108
Bst2UI CCWGG 2 cut(s) 124, 253
Bst4CI ACNGT 2 cut(s) 727, 821
Bst6I CTCTTC 2 cut(s) 1127, 1200
BstACI GRCGYC 1 cut(s) 691
BstAFI CTTAAG 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 1278
BstC8I GCNNGC 3 cut(s) 167, 919, 1230
BstDEI CTNAG 5 cut(s) 65, 647, 666, 746, 1167
BstDSI CCRYGG 2 cut(s) 11, 820
BstF5I GGATG 4 cut(s) 367, 946, 1115, 1177
BstFNI CGCG 2 cut(s) 134, 176
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 4 cut(s) 189, 258, 268, 313
BstMAI GTCTC 2 cut(s) 248, 276
BstMBI GATC 4 cut(s) 186, 255, 265, 310
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 102, 133, 788
BstNI CCWGG 2 cut(s) 124, 253
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 7 cut(s) 122, 143, 159, 160, 224, 251, 1136
BstUI CGCG 2 cut(s) 134, 176
BstV1I GCAGC 1 cut(s) 800
BstV2I GAAGAC 1 cut(s) 619
BstX2I RGATCY 2 cut(s) 255, 265
BstXI CCANNNNNNTGG 1 cut(s) 596
BstYI RGATCY 2 cut(s) 255, 265
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 5 cut(s) 105, 208, 251, 844, 921
BtgI CCRYGG 2 cut(s) 11, 820
BtsCI GGATG 4 cut(s) 367, 946, 1115, 1177
BtsIMutI CAGTG 2 cut(s) 418, 496
Cac8I GCNNGC 3 cut(s) 167, 919, 1230
CciI TCATGA 1 cut(s) 196
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 3 cut(s) 206, 842, 1112
Cfr9I CCCGGG 1 cut(s) 160
CseI GACGC 1 cut(s) 699
Csp6I GTAC 4 cut(s) 172, 572, 743, 1279
CviAII CATG 5 cut(s) 25, 197, 511, 590, 857
CviQI GTAC 4 cut(s) 172, 572, 743, 1279
DdeI CTNAG 5 cut(s) 65, 647, 666, 746, 1167
DpnI GATC 4 cut(s) 188, 257, 267, 312
DpnII GATC 4 cut(s) 186, 255, 265, 310
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 1127, 1200
EarI CTCTTC 2 cut(s) 1127, 1200
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 114, 1108
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 1112
Eco52I CGGCCG 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 1086
Eco88I CYCGRG 2 cut(s) 160, 527
EcoRI GAATTC 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 122, 251
EcoT14I CCWWGG 2 cut(s) 114, 1108
EcoT22I ATGCAT 2 cut(s) 862, 881
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 2 cut(s) 114, 1108
FaeI CATG 5 cut(s) 28, 200, 514, 593, 860
FaqI GGGAC 1 cut(s) 29
FatI CATG 5 cut(s) 24, 196, 510, 589, 856
Fnu4HI GCNGC 5 cut(s) 8, 49, 52, 106, 789
FokI GGATG 4 cut(s) 354, 953, 1102, 1184
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 8, 49, 52, 106, 789
FspBI CTAG 5 cut(s) 270, 387, 678, 701, 1109
GlaI GCGC 1 cut(s) 135
GluI GCNGC 5 cut(s) 8, 49, 52, 106, 789
HaeIII GGCC 5 cut(s) 105, 208, 251, 844, 921
HapII CCGG 5 cut(s) 144, 161, 225, 371, 1137
HgaI GACGC 1 cut(s) 699
HhaI GCGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 691
Hin1II CATG 5 cut(s) 28, 200, 514, 593, 860
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HinfI GANTC 6 cut(s) 200, 221, 241, 620, 757, 1012
HpaII CCGG 5 cut(s) 144, 161, 225, 371, 1137
HphI GGTGA 4 cut(s) 836, 884, 1202, 1223
Hpy166II GTNNAC 3 cut(s) 1060, 1131, 1294
Hpy188I TCNGA 7 cut(s) 151, 191, 232, 422, 749, 901, 1168
Hpy188III TCNNGA 9 cut(s) 79, 184, 197, 371, 527, 678, 701, 767, 986
Hpy8I GTNNAC 3 cut(s) 1060, 1131, 1294
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 3 cut(s) 340, 1082, 1110
HpyCH4III ACNGT 2 cut(s) 727, 821
HpyCH4V TGCA 9 cut(s) 397, 536, 560, 782, 860, 879, 997, 1085, 1157
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 133, 788
HpyF3I CTNAG 5 cut(s) 65, 647, 666, 746, 1167
Hsp92I GRCGYC 1 cut(s) 691
Hsp92II CATG 5 cut(s) 28, 200, 514, 593, 860
HspAI GCGC 1 cut(s) 134
Kpn2I TCCGGA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 186, 255, 265, 310
LmnI GCTCC 5 cut(s) 4, 45, 77, 232, 499
Lsp1109I GCAGC 1 cut(s) 800
LweI GCATC 4 cut(s) 59, 547, 1094, 1144
MaeI CTAG 5 cut(s) 270, 387, 678, 701, 1109
MaeIII GTNAC 3 cut(s) 402, 642, 1067
MalI GATC 4 cut(s) 188, 257, 267, 312
MboI GATC 4 cut(s) 186, 255, 265, 310
MboII GAAGA 8 cut(s) 40, 43, 305, 619, 1058, 1114, 1217, 1252
MfeI CAATTG 2 cut(s) 660, 1274
MflI RGATCY 2 cut(s) 255, 265
MhlI GDGCHC 2 cut(s) 237, 588
MlyI GAGTC 2 cut(s) 250, 766
MmeI TCCRAC 2 cut(s) 210, 532
MnlI CCTC 9 cut(s) 463, 864, 937, 1086, 1130, 1162, 1180, 1201, 1207
Mph1103I ATGCAT 2 cut(s) 862, 881
MroI TCCGGA 1 cut(s) 370
MseI TTAA 6 cut(s) 327, 504, 516, 696, 729, 801
MslI CAYNNNNRTG 3 cut(s) 411, 594, 1152
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 5 cut(s) 144, 161, 225, 371, 1137
MspR9I CCNGG 7 cut(s) 124, 145, 161, 162, 226, 253, 1138
MunI CAATTG 2 cut(s) 660, 1274
Mva1269I GAATGC 1 cut(s) 881
MvaI CCWGG 2 cut(s) 124, 253
MvnI CGCG 2 cut(s) 134, 176
MwoI GCNNNNNNNGC 3 cut(s) 102, 133, 788
NciI CCSGG 5 cut(s) 145, 161, 162, 226, 1138
NdeII GATC 4 cut(s) 186, 255, 265, 310
NlaIII CATG 5 cut(s) 28, 200, 514, 593, 860
NlaIV GGNNCC 4 cut(s) 6, 47, 234, 267
NsiI ATGCAT 2 cut(s) 862, 881
NspI RCATGY 1 cut(s) 514
OliI CACNNNNGTG 1 cut(s) 411
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 251
PctI GAATGC 1 cut(s) 881
PfeI GAWTC 4 cut(s) 200, 221, 620, 1012
Pfl23II CGTACG 1 cut(s) 171
PkrI GCNGC 5 cut(s) 9, 50, 53, 107, 790
PleI GAGTC 2 cut(s) 249, 765
PpsI GAGTC 2 cut(s) 249, 765
Psp6I CCWGG 2 cut(s) 122, 251
PspGI CCWGG 2 cut(s) 122, 251
PspLI CGTACG 1 cut(s) 171
PspN4I GGNNCC 4 cut(s) 6, 47, 234, 267
PspPI GGNCC 3 cut(s) 206, 842, 1112
PsrI GAACNNNNNNTAC 4 cut(s) 164, 196, 726, 758
PsuI RGATCY 2 cut(s) 255, 265
RsaI GTAC 4 cut(s) 173, 573, 744, 1280
RsaNI GTAC 4 cut(s) 172, 572, 743, 1279
RseI CAYNNNNRTG 3 cut(s) 411, 594, 1152
SaqAI TTAA 6 cut(s) 327, 504, 516, 696, 729, 801
SatI GCNGC 5 cut(s) 8, 49, 52, 106, 789
Sau3AI GATC 4 cut(s) 186, 255, 265, 310
Sau96I GGNCC 3 cut(s) 206, 842, 1112
SchI GAGTC 2 cut(s) 250, 766
ScrFI CCNGG 7 cut(s) 124, 145, 161, 162, 226, 253, 1138
SduI GDGCHC 2 cut(s) 237, 588
SfaNI GCATC 4 cut(s) 59, 547, 1094, 1144
SinI GGWCC 1 cut(s) 1112
SmaI CCCGGG 1 cut(s) 162
SmiMI CAYNNNNRTG 3 cut(s) 411, 594, 1152
SmlI CTYRAG 3 cut(s) 238, 245, 503
SmoI CTYRAG 3 cut(s) 238, 245, 503
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 9 cut(s) 8, 37, 43, 49, 52, 106, 176, 218, 1254
SspI AATATT 1 cut(s) 1305
SspMI CTAG 5 cut(s) 270, 387, 678, 701, 1109
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 7 cut(s) 122, 143, 159, 160, 224, 251, 1136
StyI CCWWGG 2 cut(s) 114, 1108
TaaI ACNGT 2 cut(s) 727, 821
TaqI TCGA 6 cut(s) 156, 356, 468, 634, 966, 1152
TatI WGTACW 2 cut(s) 571, 1278
TauI GCSGC 4 cut(s) 10, 51, 54, 108
TfiI GAWTC 4 cut(s) 200, 221, 620, 1012
Tru1I TTAA 6 cut(s) 327, 504, 516, 696, 729, 801
Tru9I TTAA 6 cut(s) 327, 504, 516, 696, 729, 801
TscAI CASTG 2 cut(s) 418, 496
TseI GCWGC 1 cut(s) 788
TspDTI ATGAA 3 cut(s) 41, 213, 864
TspGWI ACGGA 4 cut(s) 64, 306, 476, 613
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 2 cut(s) 418, 496
Vha464I CTTAAG 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 1112
XapI RAATTY 2 cut(s) 464, 1049
XbaI TCTAGA 2 cut(s) 677, 700
XceI RCATGY 1 cut(s) 514
XmaI CCCGGG 1 cut(s) 160
XmaJI CCTAGG 1 cut(s) 1108
XspI CTAG 5 cut(s) 270, 387, 678, 701, 1109
Zsp2I ATGCAT 2 cut(s) 862, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.