Rw4G029280

Mannose-6-phosphate isomerase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001016
Physical Location & Seq
Forward (+)
55963514 .. 55966665
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029280.1

Sequence Viewer

Length: 1308 bp
ATGGAGCCGTGGCAGAACACCATGAAGAAGAAGCGGAGGCCCGTCCGGCTCCGCTGCTCCGTCAAGAATTACGACTGGGGCATTCTCGGCCACAATTCCAAGGTCGCCAGGCTCTTCGCTCTCAATTCCGGGTCGGACATCGACCCGGCCAAGCCCTACGCCGAGTTCTGGATCGGGACTCATGAATCCGGGCCGTCCTACGCCGAGTCCGGGTCGGAGCAGCCCCTCAGCCTCAAGTCCTGGATCGCCGAGGATCCGAGTGTTCTCGGCGAGAAGGTTCTGGACAAGTGGGGCGCCGATCTTCCGTTCTTGTGCAAGGTACTTTCGGTTCAGAAGGCTCTGTCGATACAGGCTCATCCGGATAAAGAGATGGCTAGAGTGTTGCACAAGTTGCACCCCAGTGTTTACAAGGATGATAATCACAAGCCTGAAGTGGCATTGGCGCTGACGGAGTTCGAGGCTCTTTGCGGGTTTATTAGTCTCAAGGAGCTAAAAGACATGCTTCGTACTGTTCCTGAGATTGCCGAATTGGTTGGGATGGCAGATGCAGAGCAAATTCTACTTGTTGGTGAGCAACATGAGAATGGGAAACAAAACACAGATTTAGAGTCCATGTTCAGTCGACTCATGTTGTCAAGCAAAGAGACAATTTCTAAGATGATATCCAAACTGAAAAGGCGTCTTAACCTTGAGAAAAAGAAAAGGCAATTGACAGTTAAAGAACAACTGGTACTCAGATTAGAGAATCAATATCCAGCCGATGTGGGTGCTATAGCTGCTTTTTTCCTGAACTATGTGAAGCTGAAACGTGGTGAAGCATTATATTGTGGGCCAAATGAACCCCATGCATATATTTCTGGTGAATGCGTTGAATGTATGGCTACCTCTGACAATGTTGTTCGAGCCGGCCTTACCTCAAAACAAATTGATGTTCAGACACTTCTTTCTATGCTCAAATACAGACAGGGAGCTCCTGAAATTATGCAAGGAGTTTCTATGAACGCATACACAACAAGATTTGTCCCACCTTTTGAAGAATTTGAAGTCGACTGCTGTGATCTTCCTCAGTCTGCTTCGGTAGTTTTTCCCTCCGTCATAGGACCATCCCTTTTCCTAGTCACTGCAGGGAAAGGCAGGTTTGATGCAGGCTTGGCTGAGGATGATGTCGTTACAGAAGATGATGATATAGTTGAGGAGGGCGAGGTTCTATTTGTGCCTGCCCACACCAAGATTAGCATTACTGCTAGATCAACCGAACTACAATTGTATAGGGTGGGGGCGAACAGCACATTTTTTAAGGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 ko01130 map00051 map00520 map01100 map01110 map01130
Pfam Domains
Protein Families
PMI_typeI

Protein Analysis

435

Amino Acids

48.58

Weight (kDa)

5.73

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMI_typeI_cat PF20511 16 - 158 2.6e-45 Phosphomannose isomerase type I, catalytic domain
PMI_typeI_hel PF20512 189 - 263 4e-11 Phosphomannose isomerase type I, helical insertion domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1125
AccB1I GGYRCC 1 cut(s) 293
AccI GTMKAC 2 cut(s) 622, 1047
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 3 cut(s) 34, 52, 468
AclWI GGATC 4 cut(s) 179, 248, 251, 261
AcoI YGGCCR 2 cut(s) 88, 147
AcsI RAATTY 2 cut(s) 555, 1037
AcuI CTGAAG 1 cut(s) 450
AcyI GRCGYC 2 cut(s) 294, 679
AfaI GTAC 3 cut(s) 321, 508, 732
AfiI CCNNNNNNNGG 2 cut(s) 168, 210
AgsI TTSAA 3 cut(s) 872, 1034, 1043
AjnI CCWGG 2 cut(s) 107, 239
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 4 cut(s) 490, 776, 802, 971
AluI AGCT 4 cut(s) 490, 776, 802, 971
Alw21I GWGCWC 1 cut(s) 973
Alw26I GTCTC 2 cut(s) 485, 638
AlwI GGATC 4 cut(s) 179, 248, 251, 261
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 6 cut(s) 38, 88, 147, 191, 830, 907
ApeKI GCWGC 3 cut(s) 54, 220, 776
ApoI RAATTY 2 cut(s) 555, 1037
AspLEI GCGC 2 cut(s) 296, 445
AspS9I GGNCC 4 cut(s) 39, 191, 830, 1100
AsuC2I CCSGG 4 cut(s) 130, 146, 190, 211
AsuHPI GGTGA 3 cut(s) 581, 824, 872
AvaII GGWCC 1 cut(s) 1100
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 973
Bbv12I GWGCWC 1 cut(s) 973
BbvCI CCTCAGC 2 cut(s) 227, 1155
BbvI GCAGC 3 cut(s) 41, 232, 763
BccI CCATC 3 cut(s) 364, 532, 1111
BceAI ACGGC 1 cut(s) 178
BcgI CGANNNNNNTGC 2 cut(s) 749, 783
BciT130I CCWGG 2 cut(s) 109, 241
BcnI CCSGG 4 cut(s) 130, 146, 190, 211
BcoDI GTCTC 2 cut(s) 485, 638
BfaI CTAG 3 cut(s) 375, 1115, 1245
BfmI CTRYAG 2 cut(s) 771, 1122
BfoI RGCGCY 2 cut(s) 297, 446
BfuAI ACCTGC 1 cut(s) 1125
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 3 cut(s) 55, 221, 777
BlsI GCNGC 3 cut(s) 56, 222, 778
Bme1390I CCNGG 6 cut(s) 109, 130, 146, 190, 211, 241
Bme18I GGWCC 1 cut(s) 1100
BmgT120I GGNCC 4 cut(s) 39, 191, 830, 1100
BmiI GGNNCC 4 cut(s) 6, 50, 255, 295
BmrFI CCNGG 6 cut(s) 109, 130, 146, 190, 211, 241
BmrI ACTGGG 2 cut(s) 85, 393
BmsI GCATC 2 cut(s) 535, 1132
BmuI ACTGGG 2 cut(s) 85, 393
Bpu10I CCTNAGC 2 cut(s) 227, 1155
BpuEI CTTGAG 3 cut(s) 218, 467, 710
BpuMI CCSGG 4 cut(s) 130, 146, 190, 211
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 2 cut(s) 294, 679
BsaJI CCNNGG 3 cut(s) 8, 99, 249
BsaWI WCCGGW 1 cut(s) 358
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 210
Bse118I RCCGGY 1 cut(s) 905
Bse1I ACTGG 3 cut(s) 80, 399, 732
Bse8I GATNNNNATC 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 2 cut(s) 109, 241
BseDI CCNNGG 3 cut(s) 8, 99, 249
BseGI GGATG 5 cut(s) 355, 418, 543, 1103, 1165
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 168, 210
BseMII CTCAG 5 cut(s) 241, 507, 748, 1079, 1146
BseNI ACTGG 3 cut(s) 80, 399, 732
BseRI GAGGAG 1 cut(s) 1208
BseXI GCAGC 3 cut(s) 41, 232, 763
BshFI GGCC 6 cut(s) 40, 90, 149, 193, 832, 909
BshNI GGYRCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 973
BsiSI CCGG 7 cut(s) 46, 129, 146, 189, 210, 359, 906
BslFI GGGAC 2 cut(s) 190, 1007
BslI CCNNNNNNNGG 2 cut(s) 168, 210
BsmAI GTCTC 2 cut(s) 485, 638
BsmFI GGGAC 2 cut(s) 190, 1007
BsmI GAATGC 2 cut(s) 81, 869
BsnI GGCC 6 cut(s) 40, 90, 149, 193, 832, 909
Bsp1286I GDGCHC 1 cut(s) 973
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
BspACI CCGC 3 cut(s) 34, 52, 468
BspANI GGCC 6 cut(s) 40, 90, 149, 193, 832, 909
BspCNI CTCAG 5 cut(s) 240, 508, 747, 1078, 1147
BspEI TCCGGA 1 cut(s) 358
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 4 cut(s) 6, 50, 255, 295
BspMAI CTGCAG 1 cut(s) 1126
BspMI ACCTGC 1 cut(s) 1125
BspPI GGATC 4 cut(s) 179, 248, 251, 261
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 905
BsrI ACTGG 3 cut(s) 80, 399, 732
BssAI RCCGGY 1 cut(s) 905
BssECI CCNNGG 3 cut(s) 8, 99, 249
BssMI GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
BssNI GRCGYC 2 cut(s) 294, 679
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 109, 241
Bst4CI ACNGT 2 cut(s) 511, 715
Bst6I CTCTTC 1 cut(s) 119
BstACI GRCGYC 2 cut(s) 294, 679
BstAPI GCANNNNNTGC 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 907, 1147, 1218
BstDEI CTNAG 6 cut(s) 227, 516, 654, 734, 1065, 1155
BstDSI CCRYGG 1 cut(s) 8
BstF5I GGATG 5 cut(s) 355, 418, 543, 1103, 1165
BstH2I RGCGCY 2 cut(s) 297, 446
BstHHI GCGC 2 cut(s) 296, 445
BstKTI GATC 6 cut(s) 174, 246, 256, 301, 1060, 1250
BstMAI GTCTC 2 cut(s) 485, 638
BstMBI GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
BstMWI GCNNNNNNNGC 5 cut(s) 46, 87, 391, 776, 1151
BstNI CCWGG 2 cut(s) 109, 241
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 6 cut(s) 107, 128, 144, 188, 209, 239
BstSFI CTRYAG 2 cut(s) 771, 1122
BstV1I GCAGC 3 cut(s) 41, 232, 763
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 6 cut(s) 40, 90, 149, 193, 832, 909
BtgI CCRYGG 1 cut(s) 8
BtsCI GGATG 5 cut(s) 355, 418, 543, 1103, 1165
BtsI GCAGTG 1 cut(s) 1119
BtsIMutI CAGTG 2 cut(s) 406, 1119
BveI ACCTGC 1 cut(s) 1125
Cac8I GCNNGC 3 cut(s) 907, 1147, 1218
CciI TCATGA 1 cut(s) 181
CfoI GCGC 2 cut(s) 296, 445
Cfr10I RCCGGY 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 39, 191, 830, 1100
CseI GACGC 1 cut(s) 668
Csp6I GTAC 3 cut(s) 320, 507, 731
CviAII CATG 7 cut(s) 22, 182, 499, 578, 613, 628, 845
CviQI GTAC 3 cut(s) 320, 507, 731
DdeI CTNAG 6 cut(s) 227, 516, 654, 734, 1065, 1155
DinI GGCGCC 1 cut(s) 295
DpnI GATC 6 cut(s) 173, 245, 255, 300, 1059, 1249
DpnII GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
EaeI YGGCCR 2 cut(s) 88, 147
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 971
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 973
Eco32I GATATC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 1100
Eco53kI GAGCTC 1 cut(s) 971
Eco57I CTGAAG 1 cut(s) 450
EcoICRI GAGCTC 1 cut(s) 971
EcoRII CCWGG 2 cut(s) 107, 239
EcoRV GATATC 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 99
EcoT22I ATGCAT 1 cut(s) 850
EcoT38I GRGCYC 1 cut(s) 973
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 7 cut(s) 25, 185, 502, 581, 616, 631, 848
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 2 cut(s) 190, 1007
FatI CATG 7 cut(s) 21, 181, 498, 577, 612, 627, 844
FauI CCCGC 1 cut(s) 461
FblI GTMKAC 2 cut(s) 622, 1047
Fnu4HI GCNGC 3 cut(s) 55, 221, 777
FokI GGATG 5 cut(s) 342, 425, 550, 1090, 1172
FriOI GRGCYC 1 cut(s) 973
Fsp4HI GCNGC 3 cut(s) 55, 221, 777
FspBI CTAG 3 cut(s) 375, 1115, 1245
GlaI GCGC 2 cut(s) 295, 444
GluI GCNGC 3 cut(s) 55, 221, 777
HaeII RGCGCY 2 cut(s) 297, 446
HaeIII GGCC 6 cut(s) 40, 90, 149, 193, 832, 909
HapII CCGG 7 cut(s) 46, 129, 146, 189, 210, 359, 906
HgaI GACGC 1 cut(s) 668
HhaI GCGC 2 cut(s) 296, 445
Hin1I GRCGYC 2 cut(s) 294, 679
Hin1II CATG 7 cut(s) 25, 185, 502, 581, 616, 631, 848
Hin6I GCGC 2 cut(s) 294, 443
HinP1I GCGC 2 cut(s) 294, 443
HincII GTYRAC 2 cut(s) 623, 1048
HindII GTYRAC 2 cut(s) 623, 1048
HinfI GANTC 6 cut(s) 178, 185, 206, 608, 624, 745
HpaII CCGG 7 cut(s) 46, 129, 146, 189, 210, 359, 906
HphI GGTGA 3 cut(s) 581, 824, 872
Hpy166II GTNNAC 3 cut(s) 406, 623, 1048
Hpy188I TCNGA 7 cut(s) 136, 217, 258, 333, 737, 889, 936
Hpy188III TCNNGA 9 cut(s) 64, 169, 175, 182, 281, 359, 515, 787, 974
Hpy8I GTNNAC 3 cut(s) 406, 623, 1048
HpyAV CCTTC 2 cut(s) 268, 328
HpyCH4III ACNGT 2 cut(s) 511, 715
HpyCH4IV ACGT 1 cut(s) 808
HpyCH4V TGCA 8 cut(s) 315, 385, 394, 548, 848, 985, 1124, 1145
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 87, 391, 776, 1151
HpyF3I CTNAG 6 cut(s) 227, 516, 654, 734, 1065, 1155
HpySE526I ACGT 1 cut(s) 808
Hsp92I GRCGYC 2 cut(s) 294, 679
Hsp92II CATG 7 cut(s) 25, 185, 502, 581, 616, 631, 848
HspAI GCGC 2 cut(s) 294, 443
KasI GGCGCC 1 cut(s) 293
Kpn2I TCCGGA 1 cut(s) 358
KroI GCCGGC 1 cut(s) 905
KroNI GCCGGC 1 cut(s) 907
Kzo9I GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 7 cut(s) 4, 54, 62, 217, 487, 968, 976
Lsp1109I GCAGC 3 cut(s) 41, 232, 763
LweI GCATC 2 cut(s) 535, 1132
MaeI CTAG 3 cut(s) 375, 1115, 1245
MaeII ACGT 1 cut(s) 808
MaeIII GTNAC 2 cut(s) 1117, 1168
MalI GATC 6 cut(s) 173, 245, 255, 300, 1059, 1249
MboI GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
MboII GAAGA 7 cut(s) 37, 40, 106, 293, 1046, 1052, 1187
MfeI CAATTG 2 cut(s) 707, 1262
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 1 cut(s) 973
Mly113I GGCGCC 1 cut(s) 294
MlyI GAGTC 4 cut(s) 172, 215, 617, 618
MmeI TCCRAC 2 cut(s) 114, 195
Mph1103I ATGCAT 1 cut(s) 850
MroI TCCGGA 1 cut(s) 358
MroNI GCCGGC 1 cut(s) 905
MseI TTAA 3 cut(s) 684, 717, 1296
MslI CAYNNNNRTG 2 cut(s) 399, 582
MspA1I CMGCKG 1 cut(s) 54
MspI CCGG 7 cut(s) 46, 129, 146, 189, 210, 359, 906
MspR9I CCNGG 6 cut(s) 109, 130, 146, 190, 211, 241
MunI CAATTG 2 cut(s) 707, 1262
Mva1269I GAATGC 2 cut(s) 81, 869
MvaI CCWGG 2 cut(s) 109, 241
MwoI GCNNNNNNNGC 5 cut(s) 46, 87, 391, 776, 1151
NaeI GCCGGC 1 cut(s) 907
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 4 cut(s) 130, 146, 190, 211
NdeII GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
NgoMIV GCCGGC 1 cut(s) 905
NlaIII CATG 7 cut(s) 25, 185, 502, 581, 616, 631, 848
NlaIV GGNNCC 4 cut(s) 6, 50, 255, 295
NmeAIII GCCGAG 5 cut(s) 66, 187, 229, 246, 274
NmuCI GTSAC 1 cut(s) 1117
NsiI ATGCAT 1 cut(s) 850
NspI RCATGY 1 cut(s) 502
OliI CACNNNNGTG 1 cut(s) 399
PagI TCATGA 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 119
PctI GAATGC 2 cut(s) 81, 869
PdiI GCCGGC 1 cut(s) 907
PfeI GAWTC 2 cut(s) 185, 745
PfoI TCCNGGA 1 cut(s) 239
PkrI GCNGC 3 cut(s) 56, 222, 778
PleI GAGTC 4 cut(s) 172, 214, 616, 618
PluTI GGCGCC 1 cut(s) 297
PpsI GAGTC 4 cut(s) 172, 214, 616, 618
Psp124BI GAGCTC 1 cut(s) 973
Psp6I CCWGG 2 cut(s) 107, 239
PspGI CCWGG 2 cut(s) 107, 239
PspN4I GGNNCC 4 cut(s) 6, 50, 255, 295
PspPI GGNCC 4 cut(s) 39, 191, 830, 1100
PsrI GAACNNNNNNTAC 4 cut(s) 312, 344, 714, 746
PstI CTGCAG 1 cut(s) 1126
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 3 cut(s) 321, 508, 732
RsaNI GTAC 3 cut(s) 320, 507, 731
RseI CAYNNNNRTG 2 cut(s) 399, 582
SacI GAGCTC 1 cut(s) 973
SalI GTCGAC 2 cut(s) 621, 1046
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 3 cut(s) 684, 717, 1296
SatI GCNGC 3 cut(s) 55, 221, 777
Sau3AI GATC 6 cut(s) 171, 243, 253, 298, 1057, 1247
Sau96I GGNCC 4 cut(s) 39, 191, 830, 1100
SchI GAGTC 4 cut(s) 172, 215, 617, 618
ScrFI CCNGG 6 cut(s) 109, 130, 146, 190, 211, 241
SduI GDGCHC 1 cut(s) 973
SfaNI GCATC 2 cut(s) 535, 1132
SfcI CTRYAG 2 cut(s) 771, 1122
SfoI GGCGCC 1 cut(s) 295
SinI GGWCC 1 cut(s) 1100
SmiMI CAYNNNNRTG 2 cut(s) 399, 582
SmlI CTYRAG 3 cut(s) 233, 482, 689
SmoI CTYRAG 3 cut(s) 233, 482, 689
SsiI CCGC 3 cut(s) 34, 52, 468
SspDI GGCGCC 1 cut(s) 293
SspMI CTAG 3 cut(s) 375, 1115, 1245
SstI GAGCTC 1 cut(s) 973
StyD4I CCNGG 6 cut(s) 107, 128, 144, 188, 209, 239
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 511, 715
TaiI ACGT 1 cut(s) 811
TaqI TCGA 6 cut(s) 141, 344, 456, 622, 901, 1047
TfiI GAWTC 2 cut(s) 185, 745
Tru1I TTAA 3 cut(s) 684, 717, 1296
Tru9I TTAA 3 cut(s) 684, 717, 1296
TscAI CASTG 2 cut(s) 406, 1126
TseFI GTSAC 1 cut(s) 1117
TseI GCWGC 3 cut(s) 54, 220, 776
Tsp45I GTSAC 1 cut(s) 1117
TspDTI ATGAA 4 cut(s) 38, 198, 852, 1013
TspGWI ACGGA 4 cut(s) 49, 294, 464, 1081
TspRI CASTG 2 cut(s) 406, 1126
VpaK11BI GGWCC 1 cut(s) 1100
XapI RAATTY 2 cut(s) 555, 1037
XceI RCATGY 1 cut(s) 502
XmiI GTMKAC 2 cut(s) 622, 1047
XspI CTAG 3 cut(s) 375, 1115, 1245
Zsp2I ATGCAT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.