AT3G54450

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000830
Physical Location & Seq
Forward (+)
20158440 .. 20162005
3566 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54450.1

Sequence Viewer

Length: 1668 bp
ATGGCAGATTCGACCTCCCTCATCAATAAACGAACCAAAGGAGGCTGGAACGCTGCCCTTTTCATCATCGTTGTGGAGATAGCAGAGAGATTTGCGTTCTACGGTCTTGCAAGCAACCTAATAACGTTTCTAACAAACGAACTTGGCCAGTCCACGGCCACCGCAGCCAAAAACATCAACACATGGATCGGTGTTTCTTGCATGTTCCCCATTCTCGGGGCGTTTCTAGCTGACTCCATCCTAGGCCGCTTCAAAACCGTTCTCTTGACCTCTTTCATCTATCTCTTGGGGATTGTCATGTTGCCATTGTCGGTGACGGTGGTGGCGCGGCGGATGAGAGAAAAAGTGTTCTTTATGGCATTGTATGTGATGGCGGTTGGAGAAGGAGGGCATAAACCATGCGTCATGACCTTTGCAGCTGACCAGTTTGGAGAGGCCAATGCGGAAGAGAAAGCAGCAAAGACATCTTTCTTCAATTATTGGTATATGGCCATAGTCCTCGCTTCTTCCATAGCTGTTCTTGCACTCATCTTCATTCAGGAACGAGTGAGTTGGTCACTAGGATTCAGCATAATAGCAGGAAGTGTGGTGATAGCTATAGTGATATTTCTGATAGGAATTCCAAAATATCGAAAACAAGTTCCGGTGGGAAGTCCCTTCACGAGGGTTGCTCAGGTCATGGTGGCTGCTCTGAAAAAATGGCGATTGAGCTCCACGCGCCACCACTACGGCCTCTGCTACGAGGAGGAGGATGAGCACAAGTTGGAGTCAACAAATTCTAATCAAGTATATCTTCTTGCCAGAACAAATCAATTCAGATTTCTTGATAAAGCTACAATTATTGACGAGATTGATCATAACAAAAATAGAAACCCGTGGAGGTTATGTACTGTGAACCAAGTGGAAGAAGTAAAGCTAATATTGAGGCTCATACCAATTTGGATAAGCTTGATAATGTTCTGTGCCACACTCACTCAGCTCAACACATTCTTTTTAAAGCAAGGAAGTATGATGGACAGAACCATTGGGAATCACTTCACCATTCCTCCTGCTGCTTTCCAAAGCATTGTTGGTGTCACTATCCTCATCCTTATTCCTCTTTATGACCGAGTTTTTGTCCCTATGGTTCGTAAGATCACCAATCACCATTCTGGAATCACATCTCTCCAGAGAATCGGTGTTGGCTTATTTGTTGCTACTTTCAATATGGTGATATGTGGGCTTGTGGAGGCAAAGAGACTTAAAGTTGCAAGAGATCATGGATTAATCGACTCTCCAAAAGAAGTTGTTCCAATGAGTAGCTTGTGGTTACTGCCCCAATACATACTAGTTGGTATTGGAGACGTTTTCACTATTGTTGGTATGCAAGAGCTTTTCTATGATCAAATGCCTGAAACTATGAGAAGTATTGGTGCAGCGATCTTTATTAGCGTTGTTGGAGTCGGGAGTTTTGTTAGTACCGGGATTATATCTACCGTTCAAACAATCAGCAAAAGCCACGGTGAAGAATGGCTCGTCAACAACCTTAACAGAGCACATCTTGATTACTACTATTGGATAATCGCATCGCTAAATGCTGTGAGCCTCTGCTTTTATCTGTTTATAGCTAACCATTTTCTCTACAAGAAACTGCAAGACAAAGATGATGATGTTGAAAGCGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

555

Amino Acids

62.3

Weight (kDa)

8.88

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 30 - 486 8.2e-14 Major Facilitator Superfamily
PTR2 PF00854 84 - 499 1.2e-90 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 328, 718
AciI CCGC 6 cut(s) 162, 247, 328, 331, 374, 443
AclI AACGTT 1 cut(s) 125
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 3 cut(s) 145, 156, 489
AcsI RAATTY 2 cut(s) 618, 775
AfaI GTAC 2 cut(s) 889, 1459
AfiI CCNNNNNNNGG 4 cut(s) 154, 215, 216, 663
AgsI TTSAA 5 cut(s) 253, 475, 1204, 1481, 1655
AhlI ACTAGT 1 cut(s) 1327
Alw21I GWGCWC 3 cut(s) 713, 759, 1537
Alw26I GTCTC 2 cut(s) 1231, 1335
AlwI GGATC 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 6 cut(s) 145, 156, 244, 435, 489, 730
ApeKI GCWGC 7 cut(s) 53, 164, 416, 455, 686, 1052, 1415
ApoI RAATTY 2 cut(s) 618, 775
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AseI ATTAAT 1 cut(s) 1265
Asp700I GAANNNNTTC 2 cut(s) 1034, 1287
AspA2I CCTAGG 1 cut(s) 241
AspLEI GCGC 2 cut(s) 328, 720
AsuC2I CCSGG 1 cut(s) 1462
AsuHPI GGTGA 7 cut(s) 325, 601, 1030, 1129, 1136, 1222, 1514
AvaI CYCGRG 1 cut(s) 215
AvrII CCTAGG 1 cut(s) 241
BalI TGGCCA 2 cut(s) 147, 491
BanII GRGCYC 1 cut(s) 713
BauI CACGAG 1 cut(s) 661
Bbv12I GWGCWC 3 cut(s) 713, 759, 1537
BbvI GCAGC 7 cut(s) 40, 176, 428, 467, 673, 1039, 1427
BccI CCATC 3 cut(s) 245, 364, 1006
BceAI ACGGC 2 cut(s) 171, 745
BclI TGATCA 2 cut(s) 853, 1381
BcnI CCSGG 1 cut(s) 1462
BcoDI GTCTC 2 cut(s) 1231, 1335
BcuI ACTAGT 1 cut(s) 1327
BfaI CTAG 4 cut(s) 227, 242, 560, 1328
BfmI CTRYAG 1 cut(s) 597
BisI GCNGC 9 cut(s) 54, 165, 247, 329, 417, 456, 687, 1053, 1416
BlnI CCTAGG 1 cut(s) 241
BlsI GCNGC 9 cut(s) 55, 166, 248, 330, 418, 457, 688, 1054, 1417
Bme1390I CCNGG 1 cut(s) 1462
BmeT110I CYCGRG 1 cut(s) 215
BmrFI CCNGG 1 cut(s) 1462
BmsI GCATC 1 cut(s) 1574
BplI GAGNNNNNCTC 2 cut(s) 655, 687
BpmI CTGGAG 1 cut(s) 1151
Bpu10I CCTNAGC 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 1462
BsaJI CCNNGG 4 cut(s) 153, 241, 875, 1498
BsaWI WCCGGW 1 cut(s) 643
BsaXI ACNNNNNCTCC 4 cut(s) 871, 901, 1030, 1060
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 215, 216, 663
Bse1I ACTGG 2 cut(s) 148, 424
BseDI CCNNGG 4 cut(s) 153, 241, 875, 1498
BseGI GGATG 4 cut(s) 237, 339, 757, 1086
BseLI CCNNNNNNNGG 4 cut(s) 154, 215, 216, 663
BseMII CTCAG 2 cut(s) 686, 989
BseNI ACTGG 2 cut(s) 148, 424
BseRI GAGGAG 2 cut(s) 758, 761
BseXI GCAGC 7 cut(s) 40, 176, 428, 467, 673, 1039, 1427
BsgI GTGCAG 1 cut(s) 1434
Bsh1236I CGCG 2 cut(s) 328, 718
BshFI GGCC 6 cut(s) 147, 158, 246, 437, 491, 732
BsiHKAI GWGCWC 3 cut(s) 713, 759, 1537
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 2 cut(s) 644, 1461
BslFI GGGAC 2 cut(s) 639, 1103
BslI CCNNNNNNNGG 4 cut(s) 154, 215, 216, 663
BsmAI GTCTC 2 cut(s) 1231, 1335
BsmBI CGTCTC 1 cut(s) 1335
BsmFI GGGAC 2 cut(s) 639, 1103
BsnI GGCC 6 cut(s) 147, 158, 246, 437, 491, 732
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 713, 759, 1537
Bsp143I GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
BspACI CCGC 6 cut(s) 162, 247, 328, 331, 374, 443
BspANI GGCC 6 cut(s) 147, 158, 246, 437, 491, 732
BspCNI CTCAG 2 cut(s) 685, 988
BspFNI CGCG 2 cut(s) 328, 718
BspHI TCATGA 1 cut(s) 405
BspPI GGATC 1 cut(s) 194
BsrI ACTGG 2 cut(s) 148, 424
BssECI CCNNGG 4 cut(s) 153, 241, 875, 1498
BssMI GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
BssSI CACGAG 1 cut(s) 661
BssT1I CCWWGG 1 cut(s) 241
Bst2BI CACGAG 1 cut(s) 661
Bst4CI ACNGT 6 cut(s) 104, 259, 319, 892, 1477, 1502
Bst6I CTCTTC 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 2 cut(s) 672, 975
BstDSI CCRYGG 3 cut(s) 153, 875, 1498
BstENI CCTNNNNNAGG 1 cut(s) 661
BstF5I GGATG 4 cut(s) 237, 339, 757, 1086
BstFNI CGCG 2 cut(s) 328, 718
BstHHI GCGC 2 cut(s) 328, 720
BstKTI GATC 6 cut(s) 189, 856, 1137, 1258, 1384, 1422
BstMAI GTCTC 2 cut(s) 1231, 1335
BstMBI GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
BstMWI GCNNNNNNNGC 4 cut(s) 164, 227, 521, 717
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 1460
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 2 cut(s) 328, 718
BstV1I GCAGC 7 cut(s) 40, 176, 428, 467, 673, 1039, 1427
BsuRI GGCC 6 cut(s) 147, 158, 246, 437, 491, 732
BtgI CCRYGG 3 cut(s) 153, 875, 1498
BtgZI GCGATG 1 cut(s) 1551
BtsCI GGATG 4 cut(s) 237, 339, 757, 1086
Cac8I GCNNGC 1 cut(s) 112
CciI TCATGA 1 cut(s) 405
CfoI GCGC 2 cut(s) 328, 720
CseI GACGC 1 cut(s) 391
Csp6I GTAC 2 cut(s) 888, 1458
CviAII CATG 7 cut(s) 183, 202, 298, 399, 406, 679, 1259
CviQI GTAC 2 cut(s) 888, 1458
DdeI CTNAG 2 cut(s) 672, 975
DpnI GATC 6 cut(s) 188, 855, 1136, 1257, 1383, 1421
DpnII GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
DraI TTTAAA 1 cut(s) 996
EaeI YGGCCR 3 cut(s) 145, 156, 489
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
EciI GGCGGA 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 711
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 713
Eco53kI GAGCTC 1 cut(s) 711
Eco88I CYCGRG 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 711
EcoNI CCTNNNNNAGG 1 cut(s) 661
EcoRI GAATTC 1 cut(s) 618
EcoT14I CCWWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 713
ErhI CCWWGG 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 1335
FaeI CATG 7 cut(s) 186, 205, 301, 402, 409, 682, 1262
FalI AAGNNNNNCTT 2 cut(s) 777, 809
FaqI GGGAC 2 cut(s) 639, 1103
FatI CATG 7 cut(s) 182, 201, 297, 398, 405, 678, 1258
FbaI TGATCA 2 cut(s) 853, 1381
Fnu4HI GCNGC 9 cut(s) 54, 165, 247, 329, 417, 456, 687, 1053, 1416
FokI GGATG 4 cut(s) 224, 346, 764, 1073
FriOI GRGCYC 1 cut(s) 713
Fsp4HI GCNGC 9 cut(s) 54, 165, 247, 329, 417, 456, 687, 1053, 1416
FspBI CTAG 4 cut(s) 227, 242, 560, 1328
GlaI GCGC 2 cut(s) 327, 719
GluI GCNGC 9 cut(s) 54, 165, 247, 329, 417, 456, 687, 1053, 1416
GsuI CTGGAG 1 cut(s) 1151
HaeIII GGCC 6 cut(s) 147, 158, 246, 437, 491, 732
HapII CCGG 2 cut(s) 644, 1461
HgaI GACGC 1 cut(s) 391
HhaI GCGC 2 cut(s) 328, 720
Hin1II CATG 7 cut(s) 186, 205, 301, 402, 409, 682, 1262
Hin6I GCGC 2 cut(s) 326, 718
HinP1I GCGC 2 cut(s) 326, 718
HincII GTYRAC 2 cut(s) 771, 1519
HindII GTYRAC 2 cut(s) 771, 1519
HindIII AAGCTT 1 cut(s) 946
HinfI GANTC 9 cut(s) 8, 233, 564, 767, 1030, 1155, 1173, 1271, 1440
HpaII CCGG 2 cut(s) 644, 1461
HphI GGTGA 7 cut(s) 325, 601, 1030, 1129, 1136, 1222, 1514
Hpy166II GTNNAC 4 cut(s) 153, 771, 895, 1519
Hpy188I TCNGA 3 cut(s) 612, 693, 818
Hpy188III TCNNGA 9 cut(s) 265, 406, 539, 661, 824, 1152, 1168, 1444, 1541
Hpy8I GTNNAC 4 cut(s) 153, 771, 895, 1519
HpyAV CCTTC 2 cut(s) 377, 667
HpyCH4III ACNGT 6 cut(s) 104, 259, 319, 892, 1477, 1502
HpyCH4IV ACGT 2 cut(s) 125, 1344
HpyCH4V TGCA 8 cut(s) 110, 201, 416, 524, 1250, 1366, 1415, 1633
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 227, 521, 717
HpyF3I CTNAG 2 cut(s) 672, 975
HpySE526I ACGT 2 cut(s) 125, 1344
Hsp92II CATG 7 cut(s) 186, 205, 301, 402, 409, 682, 1262
HspAI GCGC 2 cut(s) 326, 718
Ksp22I TGATCA 2 cut(s) 853, 1381
Kzo9I GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
LmnI GCTCC 1 cut(s) 716
Lsp1109I GCAGC 7 cut(s) 40, 176, 428, 467, 673, 1039, 1427
LweI GCATC 1 cut(s) 1574
MaeI CTAG 4 cut(s) 227, 242, 560, 1328
MaeII ACGT 2 cut(s) 125, 1344
MaeIII GTNAC 4 cut(s) 313, 555, 1075, 1308
MalI GATC 6 cut(s) 188, 855, 1136, 1257, 1383, 1421
MboI GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
MboII GAAGA 7 cut(s) 458, 463, 498, 523, 785, 917, 1517
MhlI GDGCHC 3 cut(s) 713, 759, 1537
MlsI TGGCCA 2 cut(s) 147, 491
MluCI AATT 6 cut(s) 475, 618, 775, 812, 837, 936
MluNI TGGCCA 2 cut(s) 147, 491
MlyI GAGTC 4 cut(s) 227, 776, 1265, 1449
MmeI TCCRAC 3 cut(s) 358, 744, 1417
Mox20I TGGCCA 2 cut(s) 147, 491
MroXI GAANNNNTTC 2 cut(s) 1034, 1287
MscI TGGCCA 2 cut(s) 147, 491
MseI TTAA 4 cut(s) 995, 1242, 1265, 1527
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 2 cut(s) 147, 491
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 2 cut(s) 644, 1461
MspR9I CCNGG 1 cut(s) 1462
MvnI CGCG 2 cut(s) 328, 718
MwoI GCNNNNNNNGC 4 cut(s) 164, 227, 521, 717
NciI CCSGG 1 cut(s) 1462
NdeII GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
NlaIII CATG 7 cut(s) 186, 205, 301, 402, 409, 682, 1262
NmuCI GTSAC 3 cut(s) 313, 555, 1075
NspI RCATGY 1 cut(s) 205
PagI TCATGA 1 cut(s) 405
PdmI GAANNNNTTC 2 cut(s) 1034, 1287
PfeI GAWTC 5 cut(s) 8, 564, 1030, 1155, 1173
PkrI GCNGC 9 cut(s) 55, 166, 248, 330, 418, 457, 688, 1054, 1417
PleI GAGTC 4 cut(s) 227, 775, 1265, 1448
PpsI GAGTC 4 cut(s) 227, 775, 1265, 1448
PshBI ATTAAT 1 cut(s) 1265
Psp124BI GAGCTC 1 cut(s) 713
Psp1406I AACGTT 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 889, 1459
RsaNI GTAC 2 cut(s) 888, 1458
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 713
SaqAI TTAA 4 cut(s) 995, 1242, 1265, 1527
SatI GCNGC 9 cut(s) 54, 165, 247, 329, 417, 456, 687, 1053, 1416
Sau3AI GATC 6 cut(s) 186, 853, 1134, 1255, 1381, 1419
SchI GAGTC 4 cut(s) 227, 776, 1265, 1449
ScrFI CCNGG 1 cut(s) 1462
SduI GDGCHC 3 cut(s) 713, 759, 1537
SfaNI GCATC 1 cut(s) 1574
SfcI CTRYAG 1 cut(s) 597
SmiMI CAYNNNNRTG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 1327
Sse9I AATT 6 cut(s) 475, 618, 775, 812, 837, 936
SsiI CCGC 6 cut(s) 162, 247, 328, 331, 374, 443
SspI AATATT 1 cut(s) 921
SspMI CTAG 4 cut(s) 227, 242, 560, 1328
SstI GAGCTC 1 cut(s) 713
StyD4I CCNGG 1 cut(s) 1460
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 6 cut(s) 104, 259, 319, 892, 1477, 1502
TaiI ACGT 2 cut(s) 128, 1347
TaqI TCGA 3 cut(s) 11, 631, 1269
TaqII GACCGA 1 cut(s) 1122
TasI AATT 6 cut(s) 475, 618, 775, 812, 837, 936
TatI WGTACW 1 cut(s) 887
TauI GCSGC 2 cut(s) 249, 331
TfiI GAWTC 5 cut(s) 8, 564, 1030, 1155, 1173
Tru1I TTAA 4 cut(s) 995, 1242, 1265, 1527
Tru9I TTAA 4 cut(s) 995, 1242, 1265, 1527
TseFI GTSAC 3 cut(s) 313, 555, 1075
TseI GCWGC 7 cut(s) 53, 164, 416, 455, 686, 1052, 1415
Tsp45I GTSAC 3 cut(s) 313, 555, 1075
TspDTI ATGAA 3 cut(s) 52, 265, 523
VspI ATTAAT 1 cut(s) 1265
XagI CCTNNNNNAGG 1 cut(s) 661
XapI RAATTY 2 cut(s) 618, 775
XceI RCATGY 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 1067
XmaJI CCTAGG 1 cut(s) 241
XmnI GAANNNNTTC 2 cut(s) 1034, 1287
XspI CTAG 4 cut(s) 227, 242, 560, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.